EMDB-23255: Structure of the human Mediator-bound transcription pre-initiatio...

Basic information

• Entry: Database: EMDB / ID: EMD-23255
• Title: Structure of the human Mediator-bound transcription pre-initiation complex

Sample

• Complex: Mediator-bound pre-initiation complex
  • Complex: RNA Polymerase II
    • Protein or peptide: x 12 types
  • Complex: Mediator
    • Protein or peptide: x 24 types
  • Complex: TFIIH
    • Protein or peptide: x 7 types
  • Complex: General transcription factors
    • Protein or peptide: x 8 types
  • Complex: promoter DNA
    • DNA: x 2 types
  • Complex: cyclin-H and associated factors
    • Protein or peptide: x 3 types
• Ligand: x 3 types

• Keywords: pre-initiation complex / Mediator / initiation / complex / transcription / TRANSCRIPTION-DNA complex

Function / homology

Function:

positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / microfibril binding / MMXD complex / core TFIIH complex portion of holo TFIIH complex / Cytosolic iron-sulfur cluster assembly / central nervous system myelin formation / RNA Polymerase III Chain Elongation / positive regulation of core promoter binding / RNA polymerase II core complex assembly / RNA polymerase II CTD heptapeptide repeat S5 kinase activity / positive regulation of mitotic recombination / RNA Polymerase III Transcription Termination / hair cell differentiation / hair follicle maturation / transcription factor TFIIE complex / RNA polymerase transcription factor SL1 complex / core mediator complex / nucleotide-excision repair factor 3 complex / meiotic sister chromatid cohesion / phosphatase activator activity / nucleotide-excision repair, preincision complex assembly / ventricular system development / snRNA transcription by RNA polymerase II / RNA polymerase III general transcription initiation factor activity / TFIIF-class transcription factor complex binding / regulation of transcription by RNA polymerase I / transcription factor TFIIK complex / CAK-ERCC2 complex / transcriptional start site selection at RNA polymerase II promoter / RNA polymerase I core promoter sequence-specific DNA binding / RPAP3/R2TP/prefoldin-like complex / transcription factor TFIIF complex / nuclear retinoic acid receptor binding / embryonic cleavage / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / mediator complex / DNA 5'-3' helicase / female germ cell nucleus / transcription factor TFIIA complex / Generic Transcription Pathway / UV protection / regulation of cyclin-dependent protein serine/threonine kinase activity / RNA Polymerase III Abortive And Retractive Initiation / adult heart development / Cytosolic sensors of pathogen-associated DNA / male pronucleus / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / female pronucleus / positive regulation of chromatin binding / cyclin-dependent protein serine/threonine kinase activator activity / G protein-coupled receptor internalization / germinal vesicle / RNA polymerase II general transcription initiation factor binding / nuclear vitamin D receptor binding / nuclear thyroid hormone receptor binding / [RNA-polymerase]-subunit kinase / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / transcription preinitiation complex / RNA Polymerase I Transcription Termination / cyclin-dependent protein serine/threonine kinase regulator activity / MicroRNA (miRNA) biogenesis / Viral Messenger RNA Synthesis / Signaling by FGFR2 IIIa TM / LRR domain binding / protein acetylation / RNA polymerase II general transcription initiation factor activity / cell division site / transcription factor TFIID complex / regulation of mitotic cell cycle phase transition / limb development / erythrocyte maturation / cortical actin cytoskeleton / acetyltransferase activity / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / hematopoietic stem cell proliferation / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / spinal cord development / 3'-5' DNA helicase activity / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / RSV-host interactions / bone mineralization / mRNA Capping / DNA 3'-5' helicase / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / mRNA Splicing - Minor Pathway / PIWI-interacting RNA (piRNA) biogenesis

Domain/homology:

Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med19, metazoa / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / : / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator complex subunit 16, central helical bridge / Mediator of RNA polymerase II transcription subunit 15 / : / : / ARC105/Med15 mediator subunit N-terminal KIX domain / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / : / : / : / : / : / : / Mediator complex subunit 30 / Mediator complex subunit 28 / Mediator of RNA polymerase II transcription subunit 14, RM2 / Mediator of RNA polymerase II transcription subunit 14, RM8 / Mediator of RNA polymerase II transcription subunit 14, RM6 / Med14 RM domain / Med14 RM domain / Med14 C-terminal domain / Mediator complex, subunit Med23 / Mediator complex subunit 23 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / : / Mediator complex subunit 16, N-terminal / : / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription factor TFIIE alpha subunit, C-terminal / C-terminal general transcription factor TFIIE alpha / CyclinH/Ccl1 / TFIIH subunit Tfb4/GTF2H3 / Transcription factor Tfb4 / TFIIH C1-like domain / Ssl1-like / TFIIH subunit Ssl1/p44 / Ssl1-like / TFIIH C1-like domain / TFIIH C1-like domain / TFIIH p62 subunit, N-terminal / TFIIH subunit Tfb1/GTF2H1 / TFIIH p62 subunit, N-terminal domain / BSD domain / BSD domain superfamily / BSD domain

Component:

Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB4 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 / General transcription factor IIF subunit 2 / General transcription and DNA repair factor IIH helicase subunit XPD / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / General transcription and DNA repair factor IIH helicase/translocase subunit XPB / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB1 / General transcription factor IIE subunit 1 / Transcription initiation factor IIE subunit beta / DNA-directed RNA polymerase II subunit RPB2 / General transcription factor IIH subunit 1 / General transcription factor IIF subunit 1 / DNA-directed RNA polymerase II subunit RPB9 / Cyclin-dependent kinase 7 / Cyclin-H / CDK-activating kinase assembly factor MAT1 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB11-a / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB7 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 21 / General transcription factor IIH subunit 2 / General transcription factor IIH subunit 3 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 27 / General transcription factor IIH subunit 5 / Mediator of RNA polymerase II transcription subunit 25 / General transcription factor IIH subunit 4 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 31

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 4.8 Å
• Authors: Abdella R / Talyzina A

Funding support

United States, 5 items

Organization	Grant number	Country
Howard Hughes Medical Institute (HHMI)		United States
American Cancer Society	IRG-15-173-21	United States
National Institutes of Health/National Cancer Institute (NIH/NCI)	P01-CA092584	United States
National Institutes of Health/National Cancer Institute (NIH/NCI)	U54-CA193419	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	T32-GM008382	United States

• Citation: Journal: Science / Year: 2021; Title: Structure of the human Mediator-bound transcription preinitiation complex.; Authors: R Abdella / A Talyzina / S Chen / C J Inouye / R Tjian / Y He / ; Abstract: Eukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC) composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator Mediator is recruited by transcription factors, facilitates the assembly of the PIC, and stimulates phosphorylation of the Pol II C-terminal domain (CTD) by the TFIIH subunit CDK7. Here, we present the cryo-electron microscopy structure of the human Mediator-bound PIC at a resolution below 4 angstroms. Transcription factor binding sites within Mediator are primarily flexibly tethered to the tail module. CDK7 is stabilized by multiple contacts with Mediator. Two binding sites exist for the Pol II CTD, one between the head and middle modules of Mediator and the other in the active site of CDK7, providing structural evidence for Pol II CTD phosphorylation within the Mediator-bound PIC.

History

Deposition	Jan 8, 2021	-
Header (metadata) release	Mar 24, 2021	-
Map release	Mar 24, 2021	-
Update	Oct 16, 2024	-
Current status	Oct 16, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_23255.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.059 Å

Density

Contour Level	By AUTHOR: 0.05 / Movie #1: 0.05
Minimum - Maximum	-0.0391683 - 2.1161554
Average (Standard dev.)	0.0030885686 (±0.035189796)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 100 100 100 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 444.78 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.059	1.059	1.059
M x/y/z	420	420	420
origin x/y/z	0.000	0.000	0.000
length x/y/z	444.780	444.780	444.780
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	100	100	100
NC/NR/NS	420	420	420
D min/max/mean	-0.039	2.116	0.003

Supplemental data

Sample components

Entire : Mediator-bound pre-initiation complex

• Entire: Name: Mediator-bound pre-initiation complex

Components

• Complex: Mediator-bound pre-initiation complex
  • Complex: RNA Polymerase II
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB1
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB4
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB5
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB11-a
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC4
  • Complex: Mediator
    • Protein or peptide: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 23
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 24
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 25
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 27
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 28
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 29
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 30
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
  • Complex: TFIIH
    • Protein or peptide: TFIIH basal transcription factor complex helicase XPB subunit
    • Protein or peptide: TFIIH basal transcription factor complex helicase XPD subunit
    • Protein or peptide: General transcription factor IIH subunit 1
    • Protein or peptide: General transcription factor IIH subunit 4, p52
    • Protein or peptide: General transcription factor IIH subunit 2
    • Protein or peptide: General transcription factor IIH subunit 3
    • Protein or peptide: General transcription factor IIH subunit 5
  • Complex: General transcription factors
    • Protein or peptide: Transcription initiation factor IIA subunit 1
    • Protein or peptide: Transcription initiation factor IIA subunit 2
    • Protein or peptide: Transcription initiation factor IIB
    • Protein or peptide: TATA-box-binding protein
    • Protein or peptide: General transcription factor IIE subunit 1
    • Protein or peptide: Transcription initiation factor IIE subunit beta
    • Protein or peptide: General transcription factor IIF subunit 1
    • Protein or peptide: General transcription factor IIF subunit 2
  • Complex: promoter DNA
    • DNA: super core promoter sense strand
    • DNA: super core promoter antisense strand
  • Complex: cyclin-H and associated factors
    • Protein or peptide: CDK-activating kinase assembly factor MAT1
    • Protein or peptide: Cyclin-dependent kinase 7
    • Protein or peptide: Cyclin-H
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: IRON/SULFUR CLUSTER

Supramolecule #1: Mediator-bound pre-initiation complex

• Supramolecule: Name: Mediator-bound pre-initiation complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
• Molecular weight: Theoretical: 2.7 MDa

Supramolecule #2: RNA Polymerase II

• Supramolecule: Name: RNA Polymerase II / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #5-#16
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 520 KDa

Supramolecule #3: Mediator

• Supramolecule: Name: Mediator / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1-#4, #37-#56
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.4 MDa

Supramolecule #4: TFIIH

• Supramolecule: Name: TFIIH / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #27-#33
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 500 KDa

Supramolecule #5: General transcription factors

• Supramolecule: Name: General transcription factors / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #17-#24
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 300 KDa

Supramolecule #6: promoter DNA

• Supramolecule: Name: promoter DNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #25-#26
• Source (natural): Organism: Homo sapiens (human) / Synthetically produced: Yes

Supramolecule #7: cyclin-H and associated factors

• Supramolecule: Name: cyclin-H and associated factors / type: complex / ID: 7 / Parent: 1 / Macromolecule list: #34-#36
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16

• Macromolecule: Name: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16; type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 93.136039 KDa

Sequence

String:

MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR MIHILDTEHP WDLHSIPSEH HEAITCLEW DQSGSRLLSA DADGQIKCWS MADHLANSWE SSVGSLVEGD PIVALSWLHN GVKLALHVEK SGASSFGEKF S RVKFSPSL TLFGGKPMEG WIAVTVSGLV TVSLLKPSGQ VLTSTESLCR LRGRVALADI AFTGGGNIVV ATADGSSASP VQ FYKVCVS VVSEKCRIDT EILPSLFMRC TTDLNRKDKF PAITHLKFLA RDMSEQVLLC ASSQTSSIVE CWSLRKEGLP VNN IFQQIS PVVGDKQPTI LKWRILSATN DLDRVSAVAL PKLPISLTNT DLKVASDTQF YPGLGLALAF HDGSVHIVHR LSLQ TMAVF YSSAAPRPVD EPAMKRPRTA GPAVHLKAMQ LSWTSLALVG IDSHGKLSVL RLSPSMGHPL EVGLALRHLL FLLEY CMVT GYDWWDILLH VQPSMVQSLV EKLHEEYTRQ TAALQQVLST RILAMKASLC KLSPCTVTRV CDYHTKLFLI AISSTL KSL LRPHFLNTPD KSPGDRLTEI CTKITDVDID KVMINLKTEE FVLDMNTLQA LQQLLQWVGD FVLYLLASLP NQGSLLR PG HSFLRDGTSL GMLRELMVVI RIWGLLKPSC LPVYTATSDT QDSMSLLFRL LTKLWICCRD EGPASEPDEA LVDECCLL P SQLLIPSLDW LPASDGLVSR LQPKQPLRLQ FGRAPTLPGS AATLQLDGLA RAPGQPKIDH LRRLHLGACP TEECKACTR CGCVTMLKSP NRTTAVKQWE QRWIKNCLCG GLWWRVPLSY P

UniProtKB: Mediator of RNA polymerase II transcription subunit 16

Macromolecule #2: Mediator of RNA polymerase II transcription subunit 23

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 23; type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 156.652156 KDa

Sequence

String:

METQLQSIFE EVVKTEVIEE AFPGMFMDTP EDEKTKLISC LGAFRQFWGG LSQESHEQCI QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRLVCE SLINSDTLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK VILEKILTIP NTVSSAVVQQ L LAAREVIA YILERNACLL PAYFAVTEIR KLYPEGKLPH WLLGNLVSDF VDTFRPTARI NSICGRCSLL PVVNNSGAIC NS WKLDPAT LRFPLKGLLP YDKDLFEPQT ALLRYVLEQP YSRDMVCNML GLNKQHKQRC PVLEDQLVDL VVYAMERSET EEK FDDGGT SQLLWQHLSS QLIFFVLFQF ASFPHMVLSL HQKLAGRGLI KGRDHLMWVL LQFISGSIQK NALADFLPVM KLFD LLYPE KEYIPVPDIN KPQSTHAFAM TCIWIHLNRK AQNDNSKLQI PIPHSLRLHH EFLQQSLRNK SLQMNDYKIA LLCNA YSTN SECFTLPMGA LVETIYGNGI MRIPLPGTNC MASGSITPLP MNLLDSLTVH AKMSLIHSIA TRVIKLAHAK SSVALA PAL VETYSRLLVY MEIESLGIKG FISQLLPTVF KSHAWGILHT LLEMFSYRMH HIQPHYRVQL LSHLHTLAAV AQTNQNQ LH LCVESTALRL ITALGSSEVQ PQFTRFLSDP KTVLSAESEE LNRALILTLA RATHVTDFFT GSDSIQGTWC KDILQTIM S FTPHNWASHT LSCFPGPLQA FFKQNNVPQE SRFNLKKNVE EEYRKWKSMS NENDIITHFS MQGSPPLFLC LLWKMLLET DHINQIGYRV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLL LLKPNDFRNR VSDFVKENSP EHWLQNDWHT KHMNYHKKYP EKLYFEGLAE QVDPPVQIQS PYLPIYFGNV C LRFLPVFD IVIHRFLELL PVSKSLETLL DHLGGLYKFH DRPVTYLYNT LHYYEMHLRD RAFLKRKLVH AIIGSLKDNR PQ GWCLSDT YLKCAMNARE ENPWVPDDTY YCRLIGRLVD TMAGKSPGPF PNCDWRFNEF PNPAAHALHV TCVELMALAV SGK EVGNAL LNVVLKSQPL VPRENITAWM NAIGLIITAL PEPYWIVLHD RIVSVISSPS LTSETEWVGY PFRLFDFTAC HQSY SEMSC SYTLALAHAV WHHSSIGQLS LIPKFLTEVL LPIVKTEFQL LYVYHLVGPF LQRFQQERTR CMIEIGVAFY DMLLN VDQC STHLNYMDPI CDFLYHMKYM FTGDSVKEQV EKIICNLKPA LKLRLRFITH ISKMEPAAVP PQAMNSGSPA PQSNQV PVS LPVTQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 23

Macromolecule #3: Mediator of RNA polymerase II transcription subunit 24

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 24; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 110.430164 KDa

Sequence

String:

MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA MIGPSPNPLI LSYLKYAISS QMVSYSSVLT AISKFDDFS RDLCVQALLD IMDMFCDRLS CHGKAEECIG LCRALLSALH WLLRCTAASA ERLREGLEAG TPAAGEKQLA M CLQRLEKT LSSTKNRALL HIAKLEEASS WTAIEHSLLK LGEILANLSN PQLRSQAEQC GTLIRSIPTM LSVHAEQMHK TG FPTVHAV ILLEGTMNLT GETQSLVEQL TMVKRMQHIP TPLFVLEIWK ACFVGLIESP EGTEELKWTA FTFLKIPQVL VKL KKYSHG DKDFTEDVNC AFEFLLKLTP LLDKADQRCN CDCTNFLLQE CGKQGLLSEA SVNNLMAKRK ADREHAPQQK SGEN ANIQP NIQLILRAEP TVTNILKTMD ADHSKSPEGL LGVLGHMLSG KSLDLLLAAA AATGKLKSFA RKFINLNEFT TYGSE ESTK PASVRALLFD ISFLMLCHVA QTYGSEVILS ESRTGAEVPF FETWMQTCMP EEGKILNPDH PCFRPDSTKV ESLVAL LNN SSEMKLVQMK WHEACLSISA AILEILNAWE NGVLAFESIQ KITDNIKGKV CSLAVCAVAW LVAHVRMLGL DEREKSL QM IRQLAGPLFS ENTLQFYNER VVIMNSILER MCADVLQQTA TQIKFPSTGV DTMPYWNLLP PKRPIKEVLT DIFAKVLE K GWVDSRSIHI FDTLLHMGGV YWFCNNLIKE LLKETRKEHT LRAVELLYSI FCLDMQQVTL VLLGHILPGL LTDSSKWHS LMDPPGTALA KLAVWCALSS YSSHKGQAST RQKKRHREDI EDYISLFPLD DVQPSKLMRL LSSNEDDANI LSSPTDRSMS SSLSASQLH TVNMRDPLNR VLANLFLLIS SILGSRTAGP HTQFVQWFME ECVDCLEQGG RGSVLQFMPF TTVSELVKVS A MSSPKVVL AITDLSLPLG RQVAAKAIAA L

UniProtKB: Mediator of RNA polymerase II transcription subunit 24

Macromolecule #4: Mediator of RNA polymerase II transcription subunit 25

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 25; type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 78.230344 KDa

Sequence

String:

MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTS SAYEFVTWLD GIKFMGGGGE SCSLIAEGLS TALQLFDDFK KMREQIGQTH RVCLLICNSP PYLLPAVEST T YSGCTTEN LVQQIGERGI HFSIVSPRKL PALRLLFEKA APPALLEPLQ PPTDVSQDPR HMVLVRGLVL PVGGGSAPGP LQ SKQPVPL PPAAPSGATL SAAPQQPLPP VPPQYQVPGN LSAAQVAAQN AVEAAKNQKA GLGPRFSPIT PLQQAAPGVG PPF SQAPAP QLPPGPPGAP KPPPASQPSL VSTVAPGSGL APTAQPGAPS MAGTVAPGGV SGPSPAQLGA PALGGQQSVS NKLL AWSGV LEWQEKPKPA SVDANTKLTR SLPCQVYVNH GENLKTEQWP QKLIMQLIPQ QLLTTLGPLF RNSRMVQFHF TNKDL ESLK GLYRIMGNGF AGCVHFPHTA PCEVRVLMLL YSSKKKIFMG LIPYDQSGFV NGIRQVITNH KQVQQQKLEQ QQRGMG GQQ APPGLGPILE DQARPSQNLL QLRPPQPQPQ GTVGASGATG QPQPQGTAQP PPGAPQGPPG AASGPPPPGP ILRPQNP GA NPQLRSLLLN PPPPQTGVPP PQASLHHLQP PGAPALLPPP HQGLGQPQLG PPLLHPPPAQ SWPAQLPPRA PLPGQMLL S GGPRGPVPQP GLQPSVMEDD ILMDLI

UniProtKB: Mediator of RNA polymerase II transcription subunit 25

Macromolecule #5: DNA-directed RNA polymerase II subunit RPB1

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 217.420047 KDa

Sequence

String:

MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ PEGDEDLTKE KGHGGCGRYQ PRIRRSGLEL YAEWKHVNED SQEKKILLSP ERVHEIFKRI SDEECFVLGM EP RYARPEW MIVTVLPVPP LSVRPAVVMQ GSARNQDDLT HKLADIVKIN NQLRRNEQNG AAAHVIAEDV KLLQFHVATM VDN ELPGLP RAMQKSGRPL KSLKQRLKGK EGRVRGNLMG KRVDFSARTV ITPDPNLSID QVGVPRSIAA NMTFAEIVTP FNID RLQEL VRRGNSQYPG AKYIIRDNGD RIDLRFHPKP SDLHLQTGYK VERHMCDGDI VIFNRQPTLH KMSMMGHRVR ILPWS TFRL NLSVTTPYNA DFDGDEMNLH LPQSLETRAE IQELAMVPRM IVTPQSNRPV MGIVQDTLTA VRKFTKRDVF LERGEV MNL LMFLSTWDGK VPQPAILKPR PLWTGKQIFS LIIPGHINCI RTHSTHPDDE DSGPYKHISP GDTKVVVENG ELIMGIL CK KSLGTSAGSL VHISYLEMGH DITRLFYSNI QTVINNWLLI EGHTIGIGDS IADSKTYQDI QNTIKKAKQD VIEVIEKA H NNELEPTPGN TLRQTFENQV NRILNDARDK TGSSAQKSLS EYNNFKSMVV SGAKGSKINI SQVIAVVGQQ NVEGKRIPF GFKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQL RYGEDGLAGE SVEFQNLATL KPSNKAFEKK FRFDYTNERA LRRTLQEDLV KDVLSNAHIQ NELEREFERM R EDREVLRV IFPTGDSKVV LPCNLLRMIW NAQKIFHINP RLPSDLHPIK VVEGVKELSK KLVIVNGDDP LSRQAQENAT LL FNIHLRS TLCSRRMAEE FRLSGEAFDW LLGEIESKFN QAIAHPGEMV GALAAQSLGE PATQMTLNTF HYAGVSAKNV TLG VPRLKE LINISKKPKT PSLTVFLLGQ SARDAERAKD ILCRLEHTTL RKVTANTAIY YDPNPQSTVV AEDQEWVNVY YEMP DFDVA RISPWLLRVE LDRKHMTDRK LTMEQIAEKI NAGFGDDLNC IFNDDNAEKL VLRIRIMNSD ENKMQEEEEV VDKMD DDVF LRCIESNMLT DMTLQGIEQI SKVYMHLPQT DNKKKIIITE DGEFKALQEW ILETDGVSLM RVLSEKDVDP VRTTSN DIV EIFTVLGIEA VRKALERELY HVISFDGSYV NYRHLALLCD TMTCRGHLMA ITRHGVNRQD TGPLMKCSFE ETVDVLM EA AAHGESDPMK GVSENIMLGQ LAPAGTGCFD LLLDAEKCKY GMEIPTNIPG LGAAGPTGMF FGSAPSPMGG ISPAMTPW N QGATPAYGAW SPSVGSGMTP GAAGFSPSAA SDASGFSPGY SPAWSPTPGS PGSPGPSSPY IPSPGGAMSP SYSPTSPAY EPRSPGGYTP QSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPNYS PTSPNYTPTS P SYSPTSPS YSPTSPNYTP TSPNYSPTSP SYSPTSPSYS PTSPSYSPSS PRYTPQSPTY TPSSPSYSPS SPSYSPASPK YT PTSPSYS PSSPEYTPTS PKYSPTSPKY SPTSPKYSPT SPTYSPTTPK YSPTSPTYSP TSPVYTPTSP KYSPTSPTYS PTS PKYSPT SPTYSPTSPK GSTYSPTSPG YSPTSPTYSL TSPAISPDDS DEEN

UniProtKB: DNA-directed RNA polymerase II subunit RPB1

Macromolecule #6: DNA-directed RNA polymerase II subunit RPB2

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 134.071453 KDa

Sequence

String:

MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD RDLCELNECP LDPGGYFIIN GSEKVLIAQE KMATNTVYVF AKKDSKYAYT GECRSCLENS SRPTSTIWVS ML ARGGQGA KKSAIGQRIV ATLPYIKQEV PIIIVFRALG FVSDRDILEH IIYDFEDPEM MEMVKPSLDE AFVIQEQNVA LNF IGSRGA KPGVTKEKRI KYAKEVLQKE MLPHVGVSDF CETKKAYFLG YMVHRLLLAA LGRRELDDRD HYGNKRLDLA GPLL AFLFR GMFKNLLKEV RIYAQKFIDR GKDFNLELAI KTRIISDGLK YSLATGNWGD QKKAHQARAG VSQVLNRLTF ASTLS HLRR LNSPIGRDGK LAKPRQLHNT LWGMVCPAET PEGHAVGLVK NLALMAYISV GSQPSPILEF LEEWSMENLE EISPAA IAD ATKIFVNGCW VGIHKDPEQL MNTLRKLRRQ MDIIVSEVSM IRDIREREIR IYTDAGRICR PLLIVEKQKL LLKKRHI DQ LKEREYNNYS WQDLVASGVV EYIDTLEEET VMLAMTPDDL QEKEVAYCST YTHCEIHPSM ILGVCASIIP FPDHNQSP R NTYQSAMGKQ AMGVYITNFH VRMDTLAHVL YYPQKPLVTT RSMEYLRFRE LPAGINSIVA IASYTGYNQE DSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL ESTNRRYTK RDCSTFLRTS ETGIVDQVMV TLNQEGYKFC KIRVRSVRIP QIGDKFASRH GQKGTCGIQY RQEDMPFTCE G ITPDIIIN PHAIPSRMTI GHLIECLQGK VSANKGEIGD ATPFNDAVNV QKISNLLSDY GYHLRGNEVL YNGFTGRKIT SQ IFIGPTY YQRLKHMVDD KIHSRARGPI QILNRQPMEG RSRDGGLRFG EMERDCQIAH GAAQFLRERL FEASDPYQVH VCN LCGIMA IANTRTHTYE CRGCRNKTQI SLVRMPYACK LLFQELMSMS IAPRMMSV

UniProtKB: DNA-directed RNA polymerase II subunit RPB2

Macromolecule #7: DNA-directed RNA polymerase II subunit RPB3

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 31.478148 KDa

Sequence

String:

MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP NDYVEQDDIL IVKLRKGQEL R LRAYAKKG FGKEHAKWNP TAGVAFEYDP DNALRHTVYP KPEEWPKSEY SELDEDESQA PYDPNGKPER FYYNVESCGS LR PETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

Macromolecule #8: DNA-directed RNA polymerase II subunit RPB4

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.331255 KDa

Sequence

String:

MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQ KKLHKFELAC LANLCPETAE ESKALIPSLE GRFEDEELQQ ILDDIQTKRS FQY

UniProtKB: DNA-directed RNA polymerase II subunit RPB4

Macromolecule #9: DNA-directed RNA polymerase II subunit RPB5

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.584223 KDa

Sequence

String:

MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Macromolecule #10: DNA-directed RNA polymerases I, II, and III subunit RPABC2

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC2; type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.491026 KDa

Sequence

String:

MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIITD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

Macromolecule #11: DNA-directed RNA polymerase II subunit RPB7

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.314283 KDa

Sequence

String:

MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVV TQVNKVGLFT EIGPMSCFIS RHSIPSEMEF DPNSNPPCYK TMDEDIVIQQ DDEIRLKIVG TRVDKNDIFA I GSLMDDYL GLVS

UniProtKB: DNA-directed RNA polymerase II subunit RPB7

Macromolecule #12: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.162273 KDa

Sequence

String:

MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #13: DNA-directed RNA polymerase II subunit RPB9

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.541221 KDa

Sequence

String:

MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKC GHKEAVFFQS HSARAEDAMR LYYVCTAPHC GHRWTE

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

Macromolecule #14: DNA-directed RNA polymerases I, II, and III subunit RPABC5

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5; type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.655123 KDa

Sequence

String:

MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #15: DNA-directed RNA polymerase II subunit RPB11-a

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB11-a / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.310284 KDa

Sequence

String:

MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTN AITDLISELS LLEERFRVAI KDKQEGIE

UniProtKB: DNA-directed RNA polymerase II subunit RPB11-a

Macromolecule #16: DNA-directed RNA polymerases I, II, and III subunit RPABC4

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC4; type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.018244 KDa

Sequence

String:

MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

Macromolecule #17: Transcription initiation factor IIA subunit 1

• Macromolecule: Name: Transcription initiation factor IIA subunit 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 41.544551 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQ AQPQQTVPQQ AQTQQVLIPA SQQATAPQVI VPDSKLIQHM NASNMSAAAT AATLALPAGV TPVQQILTNS G QLLQVVRA ANGAQYIFQP QQSVVLQQQV IPQMQPGGVQ APVIQQVLAP LPGGISPQTG VIIQPQQILF TGNKTQVIPT TV AAPTPAQ AQITATGQQQ PQAQPAQTQA PLVLQVDGTG DTSSEEDEDE EEDYDDDEEE DKEKDGAEDG QVEEEPLNSE DDV SDEEGQ ELFDTENVVV CQYDKIHRSK NKWKFHLKDG IMNLNGRDYI FSKAIGDAEW

UniProtKB: Transcription initiation factor IIA subunit 1

Macromolecule #18: Transcription initiation factor IIA subunit 2

• Macromolecule: Name: Transcription initiation factor IIA subunit 2 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.469091 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG SLNTYRFCDN VWTFVLNDVE FREVTELIK VDKVKIVACD GKNTGSNTTE

UniProtKB: Transcription initiation factor IIA subunit 2

Macromolecule #19: Transcription initiation factor IIB

• Macromolecule: Name: Transcription initiation factor IIB / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.877949 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTG AASFDEFGNS KYQNRRTMSS SDRAMMNAFK EITTMADRIN LPRNIVDRTN NLFKQVYEQK SLKGRANDAI A SACLYIAC RQEGVPRTFK EICAVSRISK KEIGRCFKLI LKALETSVDL ITTGDFMSRF CSNLCLPKQV QMAATHIARK AV ELDLVPG RSPISVAAAA IYMASQASAE KRTQKEIGDI AGVADVTIRQ SYRLIYPRAP DLFPTDFKFD TPVDKLPQL

UniProtKB: Transcription initiation factor IIB

Macromolecule #20: TATA-box-binding protein

• Macromolecule: Name: TATA-box-binding protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.729938 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQ QQQQQQAVAA AAVQQSTSQQ ATQGTSGQAP QLFHSQTLTT APLPGTTPLY PSPMTPMTPI TPATPASESS G IVPQLQNI VSTVNLGCKL DLKTIALRAR NAEYNPKRFA AVIMRIREPR TTALIFSSGK MVCTGAKSEE QSRLAARKYA RV VQKLGFP AKFLDFKIQN MVGSCDVKFP IRLEGLVLTH QQFSSYEPEL FPGLIYRMIK PRIVLLIFVS GKVVLTGAKV RAE IYEAFE NIYPILKGFR KTT

UniProtKB: TATA-box-binding protein

Macromolecule #21: General transcription factor IIE subunit 1

• Macromolecule: Name: General transcription factor IIE subunit 1 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.516094 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRH NYYFINYRTL VNVVKYKLDH MRRRIETDER DSTNRASFKC PVCSSTFTDL EANQLFDPMT GTFRCTFCHT E VEEDESAM PKKDARTLLA RFNEQIEPIY ALLRETEDVN LAYEILEPEP TEIPALKQSK DHAATTAGAA SLAGGHHREA WA TKGPSYE DLYTQNVVIN MDDQEDLHRA SLEGKSAKER PIWLRESTVQ GAYGSEDMKE GGIDMDAFQE REEGHAGPDD NEE VMRALL IHEKKTSSAM AGSVGAAAPV TAANGSDSES ETSESDDDSP PRPAAVAVHK REEDEEEDDE FEEVADDPIV MVAG RPFSY SEVSQRPELV AQMTPEEKEA YIAMGQRMFE DLFE

UniProtKB: General transcription factor IIE subunit 1

Macromolecule #22: Transcription initiation factor IIE subunit beta

• Macromolecule: Name: Transcription initiation factor IIE subunit beta / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.106824 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRH QRGDTHPLTL DEILDETQHL DIGLKQKQWL MTEALVNNPK IEVIDGKYAF KPKYNVRDKK ALLRLLDQHD Q RGLGGILL EDIEEALPNS QKAVKALGDQ ILFVNRPDKK KILFFNDKSC QFSVDEEFQK LWRSVTVDSM DEEKIEEYLK RQ GISSMQE SGPKKVAPIQ RRKKPASQKK RRFKTHNEHL AGVLKDYSDI TSSK

UniProtKB: Transcription initiation factor IIE subunit beta

Macromolecule #23: General transcription factor IIF subunit 1

• Macromolecule: Name: General transcription factor IIF subunit 1 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 58.343578 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLK EFRPEDQPWL LRVNGKSGRK FKGIKKGGVT ENTSYYIFTQ CPDGAFEAFP VHNWYNFTPL ARHRTLTAEE A EEEWERRN KVLNHFSIMQ QRRLKDQDQD EDEEEKEKRG RRKASELRIH DLEDDLEMSS DASDASGEEG GRVPKAKKKA PL AKGGRKK KKKKGSDDEA FEDSDDGDFE GQEVDYMSDG SSSSQEEPES KAKAPQQEEG PKGVDEQSDS SEESEEEKPP EED KEEEEE KKAPTPQEKK RRKDSSEESD SSEESDIDSE ASSALFMAKK KTPPKRERKP SGGSSRGNSR PGTPSAEGGS TSST LRAAA SKLEQGKRVS EMPAAKRLRL DTGPQSLSGK STPQPPSGKT TPNSGDVQVT EDAVRRYLTR KPMTTKDLLK KFQTK KTGL SSEQTVNVLA QILKRLNPER KMINDKMHFS LKE

UniProtKB: General transcription factor IIF subunit 1

Macromolecule #24: General transcription factor IIF subunit 2

• Macromolecule: Name: General transcription factor IIF subunit 2 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.427309 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVG GQTLTVFTES SSDKLSLEGI VVQRAECRPA ASENYMRLKR LQIEESSKPV RLSQQLDKVV TTNYKPVANH Q YNIEYERK KKEDGKRARA DKQHVLDMLF SAFEKHQYYN LKDLVDITKQ PVVYLKEILK EIGVQNVKGI HKNTWELKPE YR HYQGEEK SD

UniProtKB: General transcription factor IIF subunit 2

Macromolecule #27: TFIIH basal transcription factor complex helicase XPB subunit

• Macromolecule: Name: TFIIH basal transcription factor complex helicase XPB subunit; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 89.404734 KDa

Sequence

String:

MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG TKVDEYGAKD YRLQMPLKDD HTSRPLWVAP DGHIFLEAF SPVYKYAQDF LVAIAEPVCR PTHVHEYKLT AYSLYAAVSV GLQTSDITEY LRKLSKTGVP DGIMQFIKLC T VSYGKVKL VLKHNRYFVE SCHPDVIQHL LQDPVIRECR LRNSEGEATE LITETFTSKS AISKTAESSG GPSTSRVTDP QG KSDIPMD LFDFYEQMDK DEEEEEETQT VSFEVKQEMI EELQKRCIHL EYPLLAEYDF RNDSVNPDIN IDLKPTAVLR PYQ EKSLRK MFGNGRARSG VIVLPCGAGK SLVGVTAACT VRKRCLVLGN SAVSVEQWKA QFKMWSTIDD SQICRFTSDA KDKP IGCSV AISTYSMLGH TTKRSWEAER VMEWLKTQEW GLMILDEVHT IPAKMFRRVL TIVQAHCKLG LTATLVREDD KIVDL NFLI GPKLYEANWM ELQNNGYIAK VQCAEVWCPM SPEFYREYVA IKTKKRILLY TMNPNKFRAC QFLIKFHERR NDKIIV FAD NVFALKEYAI RLNKPYIYGP TSQGERMQIL QNFKHNPKIN TIFISKVGDT SFDLPEANVL IQISSHGGSR RQEAQRL GR VLRAKKGMVA EEYNAFFYSL VSQDTQEMAY STKRQRFLVD QGYSFKVITK LAGMEEEDLA FSTKEEQQQL LQKVLAAT D LDAEEEVVAG EFGSRSSQAS RRFGTMSSMS GADDTVYMEY HSSRSKAPSK HVHPLFKRFR K

UniProtKB: General transcription and DNA repair factor IIH helicase/translocase subunit XPB

Macromolecule #28: TFIIH basal transcription factor complex helicase XPD subunit

• Macromolecule: Name: TFIIH basal transcription factor complex helicase XPD subunit; type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 87.021078 KDa

Sequence

String:

MKLNVDGLLV YFPYDYIYPE QFSYMRELKR TLDAKGHGVL EMPSGTGKTV SLLALIMAYQ RAYPLEVTKL IYCSRTVPEI EKVIEELRK LLNFYEKQEG EKLPFLGLAL SSRKNLCIHP EVTPLRFGKD VDGKCHSLTA SYVRAQYQHD TSLPHCRFYE E FDAHGREV PLPAGIYNLD DLKALGRRQG WCPYFLARYS ILHANVVVYS YHYLLDPKIA DLVSKELARK AVVVFDEAHN ID NVCIDSM SVNLTRRTLD RCQGNLETLQ KTVLRIKETD EQRLRDEYRR LVEGLREASA ARETDAHLAN PVLPDEVLQE AVP GSIRTA EHFLGFLRRL LEYVKWRLRV QHVVQESPPA FLSGLAQRVC IQRKPLRFCA ERLRSLLHTL EITDLADFSP LTLL ANFAT LVSTYAKGFT IIIEPFDDRT PTIANPILHF SCMDASLAIK PVFERFQSVI ITSGTLSPLD IYPKILDFHP VTMAT FTMT LARVCLCPMI IGRGNDQVAI SSKFETREDI AVIRNYGNLL LEMSAVVPDG IVAFFTSYQY MESTVASWYE QGILEN IQR NKLLFIETQD GAETSVALEK YQEACENGRG AILLSVARGK VSEGIDFVHH YGRAVIMFGV PYVYTQSRIL KARLEYL RD QFQIRENDFL TFDAMRHAAQ CVGRAIRGKT DYGLMVFADK RFARGDKRGK LPRWIQEHLT DANLNLTVDE GVQVAKYF L RQMAQPFHRE DQLGLSLLSL EQLESEETLK RIEQIAQQL

UniProtKB: General transcription and DNA repair factor IIH helicase subunit XPD

Macromolecule #29: General transcription factor IIH subunit 1

• Macromolecule: Name: General transcription factor IIH subunit 1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 62.116492 KDa

Sequence

String:

MATSSEEVLL IVKKVRQKKQ DGALYLMAER IAWAPEGKDR FTISHMYADI KCQKISPEGK AKIQLQLVLH AGDTTNFHFS NESTAVKER DAVKDLLQQL LPKFKRKANK ELEEKNRMLQ EDPVLFQLYK DLVVSQVISA EEFWANRLNV NATDSSSTSN H KQDVGISA AFLADVRPQT DGCNGLRYNL TSDIIESIFR TYPAVKMKYA ENVPHNMTEK EFWTRFFQSH YFHRDRLNTG SK DLFAECA KIDEKGLKTM VSLGVKNPLL DLTALEDKPL DEGYGISSVP SASNSKSIKE NSNAAIIKRF NHHSAMVLAA GLR KQEAQN EQTSEPSNMD GNSGDADCFQ PAVKRAKLQE SIEYEDLGKN NSVKTIALNL KKSDRYYHGP TPIQSLQYAT SQDI INSFQ SIRQEMEAYT PKLTQVLSSS AASSTITALS PGGALMQGGT QQAINQMVPN DIQSELKHLY VAVGELLRHF WSCFP VNTP FLEEKVVKMK SNLERFQVTK LCPFQEKIRR QYLSTNLVSH IEEMLQTAYN KLHTWQSRRL MKKT

UniProtKB: General transcription factor IIH subunit 1

Macromolecule #30: General transcription factor IIH subunit 4, p52

• Macromolecule: Name: General transcription factor IIH subunit 4, p52 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.245156 KDa

Sequence

String:

MESTPSRGLN RVHLQCRNLQ EFLGGLSPGV LDRLYGHPAT CLAVFRELPS LAKNWVMRML FLEQPLPQAA VALWVKKEFS KAQEESTGL LSGLRIWHTQ LLPGGLQGLI LNPIFRQNLR IALLGGGKAW SDDTSQLGPD KHARDVPSLD KYAEERWEVV L HFMVGSPS AAVSQDLAQL LSQAGLMKST EPGEPPCITS AGFQFLLLDT PAQLWYFMLQ YLQTAQSRGM DLVEILSFLF QL SFSTLGK DYSVEGMSDS LLNFLQHLRE FGLVFQRKRK SRRYYPTRLA INLSSGVSGA GGTVHQPGFI VVETNYRLYA YTE SELQIA LIALFSEMLY RFPNMVVAQV TRESVQQAIA SGITAQQIIH FLRTRAHPVM LKQTPVLPPT ITDQIRLWEL ERDR LRFTE GVLYNQFLSQ VDFELLLAHA RELGVLVFEN SAKRLMVVTP AGHSDVKRFW KRQKHSS

UniProtKB: General transcription factor IIH subunit 4

Macromolecule #31: General transcription factor IIH subunit 2

• Macromolecule: Name: General transcription factor IIH subunit 2 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.481996 KDa

Sequence

String:

MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLE YFVEEYFDQN PISQIGIIVT KSKRAEKLTE LSGNPRKHIT SLKKAVDMTC HGEPSLYNSL SIAMQTLKHM P GHTSREVL IIFSSLTTCD PSNIYDLIKT LKAAKIRVSV IGLSAEVRVC TVLARETGGT YHVILDESHY KELLTHHVSP PP ASSSSEC SLIRMGFPQH TIASLSDQDA KPSFSMAHLD GNTEPGLTLG GYFCPQCRAK YCELPVECKI CGLTLVSAPH LAR SYHHLF PLDAFQEIPL EEYNGERFCY GCQGELKDQH VYVCAVCQNV FCVDCDVFVH DSLHCCPGCI HKIPAPSGV

UniProtKB: General transcription factor IIH subunit 2

Macromolecule #32: General transcription factor IIH subunit 3

• Macromolecule: Name: General transcription factor IIH subunit 3 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.416008 KDa

Sequence

String:

MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF MNRSNKLAVI ASHIQESRFL YPGKNGRLGD FFGDPGNPP EFNPSGSKDG KYELLTSANE VIVEEIKDLM TKSDIKGQHT ETLLAGSLAK ALCYIHRMNK EVKDNQEMKS R ILVIKAAE DSALQYMNFM NVIFAAQKQN ILIDACVLDS DSGLLQQACD ITGGLYLKVP QMPSLLQYLL WVFLPDQDQR SQ LILPPPV HVDYRAACFC HRNLIEIGYV CSVCLSIFCN FSPICTTCET AFKISLPPVL KAKKKKLKVS A

UniProtKB: General transcription factor IIH subunit 3

Macromolecule #33: General transcription factor IIH subunit 5

• Macromolecule: Name: General transcription factor IIH subunit 5 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.060362 KDa

Sequence

String:

MVNVLKGVLI ECDPAMKQFL LYLDESNALG KKFIIQDIDD THVFVIAELV NVLQERVGEL MDQNAFSLTQ K

UniProtKB: General transcription factor IIH subunit 5

Macromolecule #34: CDK-activating kinase assembly factor MAT1

• Macromolecule: Name: CDK-activating kinase assembly factor MAT1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.873965 KDa

Sequence

String:

MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDF PSLREYNDFL EEVEEIVFNL TNNVDLDNTK KKMEIYQKEN KDVIQKNKLK LTREQEELEE ALEVERQENE Q RRLFIQKE EQLQQILKRK NKQAFLDELE SSDLPVALLL AQHKDRSTQL EMQLEKPKPV KPVTFSTGIK MGQHISLAPI HK LEEALYE YQPLQIETYG PHVPELEMLG RLGYLNHVRA ASPQDLAGGY TSSLACHRAL QDAFSGLFWQ PS

UniProtKB: CDK-activating kinase assembly factor MAT1

Macromolecule #35: Cyclin-dependent kinase 7

• Macromolecule: Name: Cyclin-dependent kinase 7 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: cyclin-dependent kinase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.090344 KDa

Sequence

String:

MALDVKSRAK RYEKLDFLGE GQFATVYKAR DKNTNQIVAI KKIKLGHRSE AKDGINRTAL REIKLLQELS HPNIIGLLDA FGHKSNISL VFDFMETDLE VIIKDNSLVL TPSHIKAYML MTLQGLEYLH QHWILHRDLK PNNLLLDENG VLKLADFGLA K SFGSPNRA YTHQVVTRWY RAPELLFGAR MYGVGVDMWA VGCILAELLL RVPFLPGDSD LDQLTRIFET LGTPTEEQWP DM CSLPDYV TFKSFPGIPL HHIFSAAGDD LLDLIQGLFL FNPCARITAT QALKMKYFSN RPGPTPGCQL PRPNCPVETL KEQ SNPALA IKRKRTEALE QGGLPKKLIF

UniProtKB: Cyclin-dependent kinase 7

Macromolecule #36: Cyclin-H

• Macromolecule: Name: Cyclin-H / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.695473 KDa

Sequence

String:

MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKR FYLNNSVMEY HPRIIMLTCA FLACKVDEFN VSSPQFVGNL RESPLGQEKA LEQILEYELL LIQQLNFHLI V HNPYRPFE GFLIDLKTRY PILENPEILR KTADDFLNRI ALTDAYLLYT PSQIALTAIL SSASRAGITM ESYLSESLML KE NRTCLSQ LLDIMKSMRN LVKKYEPPRS EEVAVLKQKL ERCHSAELAL NVITKKRKGY EDDDYVSKKS KHEEEEWTDD DLV ESL

UniProtKB: Cyclin-H

Macromolecule #37: Mediator of RNA polymerase II transcription subunit 6

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.464627 KDa

Sequence

String:

MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQDKVR P KAKRKEEP SSIFQRQRVD ALLLDLRQKF PPKFVQLKPG EKPVPVDQTK KEAEPIPETV KPEEKETTKN VQQTVSAKGP PE KRMRLQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

Macromolecule #38: Mediator of RNA polymerase II transcription subunit 8

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.112906 KDa

Sequence

String:

MQREEKQLEA SLDALLSQVA DLKNSLGSFI CKLENEYGRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP NKQTFNPTDT NALVAAVAFG KGLSNWRPSG SSGPGQAGQP GAGTILAGTS GLQQVQMAGA PSQQQPMLSG VQ MAQAGQP GKMPSGIKTN IKSASMHPYQ R

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

Macromolecule #39: Mediator of RNA polymerase II transcription subunit 11

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 11; type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.145829 KDa

Sequence

String:

MATYSLANER LRALEDIERE IGAILQNAGT VILELSKEKT NERLLDRQAA AFTASVQHVE AELSAQIRYL TQVATGQPHE GSSYSSRKD CQMALKRVDY ARLKLSDVAR TCEQMLEN

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

Macromolecule #40: Mediator of RNA polymerase II transcription subunit 17

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 17; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 72.984047 KDa

Sequence

String:

MSGVRAVRIS IESACEKQVH EVGLDGTETY LPPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGTEGDAQEW PGAGSSADQD DEEGVVKFQ PSLWPWDSVR NNLRSALTEM CVLYDVLSIV RDKKFMTLDP VSQDALPPKQ NPQTLQLISK KKSLAGAAQI L LKGAERLT KSVTENQENK LQRDFNSELL RLRQHWKLRK VGDKILGDLS YRSAGSLFPH HGTFEVIKNT DLDLDKKIPE DY CPLDVQI PSDLEGSAYI KVSIQKQAPD IGDLGTVNLF KRPLPKSKPG SPHWQTKLEA AQNVLLCKEI FAQLSREAVQ IKS QVPHIV VKNQIISQPF PSLQLSISLC HSSNDKKSQK FATEKQCPED HLYVLEHNLH LLIREFHKQT LSSIMMPHPA SAPF GHKRM RLSGPQAFDK NEINSLQSSE GLLEKIIKQA KHIFLRSRAA ATIDSLASRI EDPQIQAHWS NINDVYESSV KVLIT SQGY EQICKSIQLQ LNIGVEQIRV VHRDGRVITL SYQEQELQDF LLSQMSQHQV HAVQQLAKVM GWQVLSFSNH VGLGPI ESI GNASAITVAS PSGDYAISVR NGPESGSKIM VQFPRNQCKD LPKSDVLQDN KWSHLRGPFK EVQWNKMEGR NFVYKME LL MSALSPCLL

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

Macromolecule #41: Mediator of RNA polymerase II transcription subunit 18

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 18; type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.69373 KDa

Sequence

String:

MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKIMVYKIF RILVPGNTDS T EALSLSYL VELSVVAPAG QDMVSDDMKN FAEQLKPLVH LEKIDPKRLM

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

Macromolecule #42: Mediator of RNA polymerase II transcription subunit 20

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 20; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.243762 KDa

Sequence

String:

MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH TPGAPAVFGN RHDAVYGPAD TMVQYMELFN KIRKQQQVPV AGIR

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

Macromolecule #43: Mediator of RNA polymerase II transcription subunit 22

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 22; type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.243713 KDa

Sequence

String:

MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRTLQ EECDRKLITL RDEISIDLYE LEEEYYSSSS SLCEANDLPL CEAYGRLDLD T DSADGLSA PLLASPEPSA GPLQVAAPAH SHAGGPGPTE HA

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

Macromolecule #44: Mediator of RNA polymerase II transcription subunit 27

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 27; type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.478742 KDa

Sequence

String:

MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR IDRMFPEMSI HLSRPNGTSA MLLVTLGKVL KVIVVMRSLF IDRTIVKGYN ENVYTEDGKL DIWSKSNYQV FQ KVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 27

Macromolecule #45: Mediator of RNA polymerase II transcription subunit 28

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 28; type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.540107 KDa

Sequence

String:

MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIAR QTECFFLQKR LQLSVQKPEQ VIKEDVSELR NELQRKDALV QKHLTKLRHW QQVLEDINVQ HKKPADIPQG S LAYLEQAS ANIPAPLKPT

UniProtKB: Mediator of RNA polymerase II transcription subunit 28

Macromolecule #46: Mediator of RNA polymerase II transcription subunit 29

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 29; type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.089801 KDa

Sequence

String:

MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNID NGQKSSDGPI QRFDKCLEEF YALCDQLELC LRLAHECLSQ SCDSAKHSPT LVPTATKPDA VQPDSLPYPQ Y LAVIKAQI SCAKDIHTAL LDCANKVTGK TPAPPAGPGG TL

UniProtKB: Mediator of RNA polymerase II transcription subunit 29

Macromolecule #47: Mediator of RNA polymerase II transcription subunit 30

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 30; type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.301229 KDa

Sequence

String:

MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLF RKLRLVYDKC NENCGGMDPI PVEQLIPYVE EDGSKNDDRA GPPRFASEER REIAEVNKKL KQKNQQLKQI M DQLRNLIW DINAMLAMRN

UniProtKB: Mediator of RNA polymerase II transcription subunit 30

Macromolecule #48: Mediator of RNA polymerase II transcription subunit 14

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 14; type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 160.787594 KDa

Sequence

String:

MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR LSTLIEFLLH RAYSELMVLT DLLPRKSDVE RKIEIVQFA SRTRQLFVRL LALVKWANNA GKVEKCAMIS SFLDQQAILF VDTADRLASL ARDALVHARL PSFAIPYAID V LTTGSYPR LPTCIRDKII PPDPITKIEK QATLHQLNQI LRHRLVTTDL PPQLANLTVA NGRVKFRVEG EFEATLTVMG DD PDVPWRL LKLEILVEDK ETGDGRALVH SMQISFIHQL VQSRLFADEK PLQDMYNCLH SFCLSLQLEV LHSQTLMLIR ERW GDLVQV ERYHAGKCLS LSVWNQQVLG RKTGTASVHK VTIKIDENDV SKPLQIFHDP PLPASDSKLV ERAMKIDHLS IEKL LIDSV HARAHQKLQE LKAILRGFNA NENSSIETAL PALVVPILEP CGNSECLHIF VDLHSGMFQL MLYGLDQATL DDMEK SVND DMKRIIPWIQ QLKFWLGQQR CKQSIKHLPT ISSETLQLSN YSTHPIGNLS KNKLFIKLTR LPQYYIVVEM LEVPNK PTQ LSYKYYFMSV NAADREDSPA MALLLQQFKE NIQDLVFRTK TGKQTRTNAK RKLSDDPCPV ESKKTKRAGE MCAFNKV LA HFVAMCDTNM PFVGLRLELS NLEIPHQGVQ VEGDGFSHAI RLLKIPPCKG ITEETQKALD RSLLDCTFRL QGRNNRTW V AELVFANCPL NGTSTREQGP SRHVYLTYEN LLSEPVGGRK VVEMFLNDWN SIARLYECVL EFARSLPDIP AHLNIFSEV RVYNYRKLIL CYGTTKGSSI SIQWNSIHQK FHISLGTVGP NSGCSNCHNT ILHQLQEMFN KTPNVVQLLQ VLFDTQAPLN AINKLPTVP MLGLTQRTNT AYQCFSILPQ SSTHIRLAFR NMYCIDIYCR SRGVVAIRDG AYSLFDNSKL VEGFYPAPGL K TFLNMFVD SNQDARRRSV NEDDNPPSPI GGDMMDSLIS QLQPPPQQQP FPKQPGTSGA YPLTSPPTSY HSTVNQSPSM MH TQSPGNL HAASSPSGAL RAPSPASFVP TPPPSSHGIS IGPGASFASP HGTLDPSSPY TMVSPSGRAG NWPGSPQVSG PSP AARMPG MSPANPSLHS PVPDASHSPR AGTSSQTMPT NMPPPRKLPQ RSWAASIPTI LTHSALNILL LPSPTPGLVP GLAG SYLCS PLERFLGSVI MRRHLQRIIQ QETLQLINSN EPGVIMFKTD ALKCRVALSP KTNQTLQLKV TPENAGQWKP DELQV LEKF FETRVAGPPF KANTLIAFTK LLGAPTHILR DCVHIMKLEL FPDQATQLKW NVQFCLTIPP SAPPIAPPGT PAVVLK SKM LFFLQLTQKT SVPPQEPVSI IVPIIYDMAS GTTQQADIPR QQNSSVAAPM MVSNILKRFA EMNPPRQGEC TIFAAVR DL MANLTLPPGG RP

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

Macromolecule #49: Mediator of RNA polymerase II transcription subunit 4

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.781424 KDa

Sequence

String:

MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKI HHEMQVLEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV PGDPRRPYPT DLEMRSGLLG QMNNPSTNGV NGHLPGDALA AGRLPDVLAP QYPWQSNDMS MNMLPPNHSS DF LLEPPGH NKENEDDVEI MSTDSSSSSS ESD

UniProtKB: Mediator of RNA polymerase II transcription subunit 4

Macromolecule #50: Mediator of RNA polymerase II transcription subunit 7

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.285281 KDa

Sequence

String:

MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ASLPDDLPHS EAGMRVKTEP MDADDSNNCT GQNEHQRENS GHRRDQIIEK DAALCVLIDE MNERP

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

Macromolecule #51: Mediator of RNA polymerase II transcription subunit 9

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.42275 KDa

Sequence

String:

MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNA LKSKFQEMRK LISTMPGIHL SPEQQQQQLQ SLREQVRTKN ELLQKYKSLC MFEIPKE

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

Macromolecule #52: Mediator of RNA polymerase II transcription subunit 10

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 10; type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.710969 KDa

Sequence

String:

MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLNQK LNFIVTGLQD IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERAL AKNEQVKGKI DTMKKFKSLL IQELSKVFPE DMAKYRSIRG EDHPPS

UniProtKB: Mediator of RNA polymerase II transcription subunit 10

Macromolecule #53: Mediator of RNA polymerase II transcription subunit 19

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 19; type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.321146 KDa

Sequence

String:

MENFTALFGA QADPPPPPTA LGFGPGKPPP PPPPPAGGGP GTAPPPTAAT APPGADKSGA GCGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILSS SFNPITGTML AGFRLHTGPL P EQCRLMHI QPPKKKNKHK HKQSRTQDPV PPETPSDSDH KKKKKKKEED PDRKRKKKEK KKKKNRHSPD HPGMGSSQAS SS SSLR

UniProtKB: Mediator of RNA polymerase II transcription subunit 19

Macromolecule #54: Mediator of RNA polymerase II transcription subunit 21

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 21; type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.575234 KDa

Sequence

String:

MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAAS LYKLEEENHE AATCLEDVVY RGDMLLEKIQ SALADIAQSQ LKTRSGTHSQ SLPDS

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

Macromolecule #55: Mediator of RNA polymerase II transcription subunit 31

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 31; type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.826116 KDa

Sequence

String:

MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKD PEYAKYLKYP QCLHMLELLQ YEHFRKELV NAQCAKFIDE QQILHWQHYS RKRMRLQQAL AEQQQQNNTS GK

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

Macromolecule #56: Mediator of RNA polymerase II transcription subunit 15

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 15; type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 86.830102 KDa

Sequence

String:

MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLT GGPAAGAAGI GMPPRGPGQS LGGMGSLGAM GQPMSLSGQP PPGTSGMAPH SMAVVSTATP QTQLQLQQVA L QQQQQQQQ FQQQQQAALQ QQQQQQQQQQ FQAQQSAMQQ QFQAVVQQQQ QLQQQQQQQQ HLIKLHHQNQ QQIQQQQQQL QR IAQLQLQ QQQQQQQQQQ QQQQQALQAQ PPIQQPPMQQ PQPPPSQALP QQLQQMHHTQ HHQPPPQPQQ PPVAQNQPSQ LPP QSQTQP LVSQAQALPG QMLYTQPPLK FVRAPMVVQQ PPVQPQVQQQ QTAVQTAQAA QMVAPGVQMI TEALAQGGMH IRAR FPPTT AVSAIPSSSI PLGRQPMAQV SQSSLPMLSS PSPGQQVQTP QSMPPPPQPS PQPGQPSSQP NSNVSSGPAP SPSSF LPSP SPQPSQSPVT ARTPQNFSVP SPGPLNTPVN PSSVMSPAGS SQAEEQQYLD KLKQLSKYIE PLRRMINKID KNEDRK KDL SKMKSLLDIL TDPSKRCPLK TLQKCEIALE KLKNDMAVPT PPPPPVPPTK QQYLCQPLLD AVLANIRSPV FNHSLYR TF VPAMTAIHGP PITAPVVCTR KRRLEDDERQ SIPSVLQGEV ARLDPKFLVN LDPSHCSNNG TVHLICKLDD KDLPSVPP L ELSVPADYPA QSPLWIDRQW QYDANPFLQS VHRCMTSRLL QLPDKHSVTA LLNTWAQSVH QACLSAA

UniProtKB: Mediator of RNA polymerase II transcription subunit 15

Macromolecule #25: super core promoter sense strand

• Macromolecule: Name: super core promoter sense strand / type: dna / ID: 25 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 31.00277 KDa

Sequence

String:

(DG)(DA)(DA)(DG)(DG)(DG)(DC)(DG)(DC)(DC) (DT)(DA)(DT)(DA)(DA)(DA)(DA)(DG)(DG)(DG) (DG)(DG)(DT)(DG)(DG)(DG)(DG)(DG)(DC) (DG)(DC)(DG)(DT)(DT)(DC)(DG)(DT)(DC)(DC) (DT) (DC)(DA)(DG)(DT)(DC)(DG)(DC)(DG) (DA)(DT)(DC)(DG)(DA)(DA)(DC)(DA)(DC)(DT) (DC)(DG) (DA)(DG)(DC)(DC)(DG)(DA)(DG) (DC)(DA)(DG)(DA)(DC)(DG)(DT)(DG)(DC)(DC) (DT)(DA)(DC) (DG)(DG)(DA)(DC)(DC)(DA) (DT)(DG)(DG)(DA)(DA)(DT)(DT)(DC)(DC)(DC) (DC)(DA)(DG)(DT)

Macromolecule #26: super core promoter antisense strand

• Macromolecule: Name: super core promoter antisense strand / type: dna / ID: 26 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.708537 KDa

Sequence

String:

(DA)(DC)(DT)(DG)(DG)(DG)(DG)(DA)(DA)(DT) (DT)(DC)(DC)(DA)(DT)(DG)(DG)(DT)(DC)(DC) (DG)(DT)(DA)(DG)(DG)(DC)(DA)(DC)(DG) (DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG)(DC) (DT) (DC)(DG)(DA)(DG)(DT)(DG)(DT)(DT) (DC)(DG)(DA)(DT)(DC)(DG)(DC)(DG)(DA)(DC) (DT)(DG) (DA)(DG)(DG)(DA)(DC)(DG)(DA) (DA)(DC)(DG)(DC)(DG)(DC)(DC)(DC)(DC)(DC) (DA)(DC)(DC) (DC)(DC)(DC)(DT)(DT)(DT) (DT)(DA)(DT)(DA)(DG)(DG)(DC)(DG)(DC)(DC) (DC)(DT)(DT)(DC)

Macromolecule #57: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 57 / Number of copies: 2 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #58: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 58 / Number of copies: 16 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #59: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 59 / Number of copies: 1 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 31.5 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: OTHER
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 156383
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD