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Structure paper

TitleThe Hantavirus Surface Glycoprotein Lattice and Its Fusion Control Mechanism.
Journal, issue, pagesCell, Vol. 183, Issue 2, Page 442-456.e16, Year 2020
Publish dateOct 15, 2020
AuthorsAlexandra Serris / Robert Stass / Eduardo A Bignon / Nicolás A Muena / Jean-Claude Manuguerra / Rohit K Jangra / Sai Li / Kartik Chandran / Nicole D Tischler / Juha T Huiskonen / Felix A Rey / Pablo Guardado-Calvo /
PubMed AbstractHantaviruses are rodent-borne viruses causing serious zoonotic outbreaks worldwide for which no treatment is available. Hantavirus particles are pleomorphic and display a characteristic square ...Hantaviruses are rodent-borne viruses causing serious zoonotic outbreaks worldwide for which no treatment is available. Hantavirus particles are pleomorphic and display a characteristic square surface lattice. The envelope glycoproteins Gn and Gc form heterodimers that further assemble into tetrameric spikes, the lattice building blocks. The glycoproteins, which are the sole targets of neutralizing antibodies, drive virus entry via receptor-mediated endocytosis and endosomal membrane fusion. Here we describe the high-resolution X-ray structures of the heterodimer of Gc and the Gn head and of the homotetrameric Gn base. Docking them into an 11.4-Å-resolution cryoelectron tomography map of the hantavirus surface accounted for the complete extramembrane portion of the viral glycoprotein shell and allowed a detailed description of the surface organization of these pleomorphic virions. Our results, which further revealed a built-in mechanism controlling Gc membrane insertion for fusion, pave the way for immunogen design to protect against pathogenic hantaviruses.
External linksCell / PubMed:32937107 / PubMed Central
MethodsEM (subtomogram averaging) / X-ray diffraction
Resolution1.902 - 15.6 Å
Structure data

11236

6zjm

EMDB-11236: Reconstruction of Tula virus surface glycoprotein lattice
PDB-6zjm: Atomic model of Andes virus glycoprotein spike tetramer generated by fitting into a Tula virus reconstruction
Method: EM (subtomogram averaging) / Resolution: 11.4 Å

EMDB-4867:
Sub-tomogram averaging of Tula virus glycoprotein spike
Method: EM (subtomogram averaging) / Resolution: 15.6 Å

PDB-6y5f:
Crystal structure of the envelope glycoprotein prefusion complex of Andes virus - Mutant H953F
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-6y5w:
Crystal structure of the envelope glycoprotein complex of Andes virus in a near postfusion conformation
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-6y62:
Crystal structure of the envelope glycoprotein complex of Maporal virus in a prefusion conformation
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6y68:
Structure of Maporal virus envelope glycoprotein Gc in postfusion conformation
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-6y6p:
Structure of Hantaan virus envelope glycoprotein Gn
Method: X-RAY DIFFRACTION / Resolution: 1.938 Å

PDB-6y6q:
Structure of Andes virus envelope glycoprotein Gc in postfusion conformation
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-6yrb:
Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 7.5
Method: X-RAY DIFFRACTION / Resolution: 2.351 Å

PDB-6yrq:
Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 4.6
Method: X-RAY DIFFRACTION / Resolution: 1.902 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-HOH:
WATER / Water

ChemComp-CD:
Unknown entry

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-IOD:
IODIDE ION / Iodide

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

Source
  • tula orthohantavirus
  • andes orthohantavirus
  • maporal virus
  • hantaan orthohantavirus
  • drosophila melanogaster (fruit fly)
KeywordsVIRAL PROTEIN / class-II fusion protein hantavirus bunyavirus prefusion complex / class-II fusion protein hantavirus bunyavirus / prefusion complex / postfusion conformation / Hantavirus / Tula / Andes / TULV / ANDV / glycoprotein / lattice / spike / fusion protein

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