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Structure paper

TitleStructure of SWI/SNF chromatin remodeller RSC bound to a nucleosome.
Journal, issue, pagesNature, Vol. 579, Issue 7799, Page 448-451, Year 2020
Publish dateMar 11, 2020
AuthorsFelix R Wagner / Christian Dienemann / Haibo Wang / Alexandra Stützer / Dimitry Tegunov / Henning Urlaub / Patrick Cramer /
PubMed AbstractChromatin-remodelling complexes of the SWI/SNF family function in the formation of nucleosome-depleted, transcriptionally active promoter regions (NDRs). In the yeast Saccharomyces cerevisiae, the ...Chromatin-remodelling complexes of the SWI/SNF family function in the formation of nucleosome-depleted, transcriptionally active promoter regions (NDRs). In the yeast Saccharomyces cerevisiae, the essential SWI/SNF complex RSC contains 16 subunits, including the ATP-dependent DNA translocase Sth1. RSC removes nucleosomes from promoter regions and positions the specialized +1 and -1 nucleosomes that flank NDRs. Here we present the cryo-electron microscopy structure of RSC in complex with a nucleosome substrate. The structure reveals that RSC forms five protein modules and suggests key features of the remodelling mechanism. The body module serves as a scaffold for the four flexible modules that we call DNA-interacting, ATPase, arm and actin-related protein (ARP) modules. The DNA-interacting module binds extra-nucleosomal DNA and is involved in the recognition of promoter DNA elements that influence RSC functionality. The ATPase and arm modules sandwich the nucleosome disc with the Snf2 ATP-coupling (SnAC) domain and the finger helix, respectively. The translocase motor of the ATPase module engages with the edge of the nucleosome at superhelical location +2. The mobile ARP module may modulate translocase-nucleosome interactions to regulate RSC activity. The RSC-nucleosome structure provides a basis for understanding NDR formation and the structure and function of human SWI/SNF complexes that are frequently mutated in cancer.
External linksNature / PubMed:32188943 / PubMed Central
MethodsEM (single particle)
Resolution15.0 Å
Structure data

10465

6tda

EMDB-10465, PDB-6tda:
Structure of SWI/SNF chromatin remodeler RSC bound to a nucleosome
Method: EM (single particle) / Resolution: 15.0 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
  • xenopus laevis (African clawed frog)
  • synthetic construct (others)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsDNA BINDING PROTEIN / Chromatin remodeler DNA binding Nucleosome binding ATPase Transcription

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