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Structure paper

TitleStructural basis of energy transfer in Porphyridium purpureum phycobilisome.
Journal, issue, pagesNature, Vol. 579, Issue 7797, Page 146-151, Year 2020
Publish dateFeb 19, 2020
AuthorsJianfei Ma / Xin You / Shan Sun / Xiaoxiao Wang / Song Qin / Sen-Fang Sui /
PubMed AbstractPhotosynthetic organisms have developed various light-harvesting systems to adapt to their environments. Phycobilisomes are large light-harvesting protein complexes found in cyanobacteria and red ...Photosynthetic organisms have developed various light-harvesting systems to adapt to their environments. Phycobilisomes are large light-harvesting protein complexes found in cyanobacteria and red algae, although how the energies of the chromophores within these complexes are modulated by their environment is unclear. Here we report the cryo-electron microscopy structure of a 14.7-megadalton phycobilisome with a hemiellipsoidal shape from the red alga Porphyridium purpureum. Within this complex we determine the structures of 706 protein subunits, including 528 phycoerythrin, 72 phycocyanin, 46 allophycocyanin and 60 linker proteins. In addition, 1,598 chromophores are resolved comprising 1,430 phycoerythrobilin, 48 phycourobilin and 120 phycocyanobilin molecules. The markedly improved resolution of our structure compared with that of the phycobilisome of Griffithsia pacifica enabled us to build an accurate atomic model of the P. purpureum phycobilisome system. The model reveals how the linker proteins affect the microenvironment of the chromophores, and suggests that interactions of the aromatic amino acids of the linker proteins with the chromophores may be a key factor in fine-tuning the energy states of the chromophores to ensure the efficient unidirectional transfer of energy.
External linksNature / PubMed:32076272
MethodsEM (single particle)
Resolution2.67 - 3.74 Å
Structure data

EMDB-34529: Un-sharpened map of phycobilisome from Porphyridium purpureum under low light
Method: EM (single particle) / Resolution: 3.74 Å

9976

6kgx

EMDB-9976, PDB-6kgx:
Structure of the phycobilisome from the red alga Porphyridium purpureum
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-9977:
Local map of the core region of the phycobilisome from P. purpureum
Method: EM (single particle) / Resolution: 2.67 Å

EMDB-9978:
Local map of the Ra region of the phycobilisome from P. purpureum
Method: EM (single particle) / Resolution: 2.92 Å

EMDB-9979:
Local map of the Rb region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 2.77 Å

EMDB-9980:
Local map of the Rc region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 2.91 Å

EMDB-9981:
Local map of the Rd region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 2.82 Å

EMDB-9982:
Local map of the Re region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 3.04 Å

EMDB-9983:
Local map of the Rf region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-9984:
Local map of the Rg region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-9985:
Local map of the Ha region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 2.85 Å

EMDB-9986:
Local map of the Hb region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 3.56 Å

EMDB-9987:
Local map of the Hc region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 3.54 Å

EMDB-9988:
Local map of the Hd region of the phycobilisome from the red alga P. purpureum
Method: EM (single particle) / Resolution: 2.77 Å

Chemicals

ChemComp-PEB:
PHYCOERYTHROBILIN

ChemComp-PUB:
PHYCOUROBILIN

ChemComp-CYC:
PHYCOCYANOBILIN

Source
  • porphyridium purpureum (eukaryote)
  • Red alga (eukaryote)
KeywordsPHOTOSYNTHESIS / phycobilisome / Complex

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