[English] 日本語
EMN Papers
- Database of articles cited by 3DEM data entries -

+
Search query

Keywords
Author
Journal
IF

-
Structure paper

TitleA human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.
Journal, issue, pagesScience, Vol. 363, Issue 6428, Page 710-714, Year 2019
Publish dateFeb 15, 2019
AuthorsSebastian M Fica / Chris Oubridge / Max E Wilkinson / Andrew J Newman / Kiyoshi Nagai /
PubMed AbstractDuring exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a ...During exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a conformation stabilized by Prp18 and Prp8. Here we present the 3.3-angstrom cryo-electron microscopy structure of a human postcatalytic spliceosome just after exon ligation. The 3'SS docks at the active site through conserved RNA interactions in the absence of Prp18. Unexpectedly, the metazoan-specific FAM32A directly bridges the 5'-exon and intron 3'SS of pre-mRNA and promotes exon ligation, as shown by functional assays. CACTIN, SDE2, and NKAP-factors implicated in alternative splicing-further stabilize the catalytic conformation of the spliceosome during exon ligation. Together these four proteins act as exon ligation factors. Our study reveals how the human spliceosome has co-opted additional proteins to modulate a conserved RNA-based mechanism for 3'SS selection and to potentially fine-tune alternative splicing at the exon ligation stage.
External linksScience / PubMed:30705154 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 5.9 Å
Structure data

4525

6qdv

EMDB-4525: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
PDB-6qdv: Human post-catalytic P complex spliceosome
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-4526:
Human post-catalytic spliceosome (P complex) stalled with DHX8 S717A mutant, overall map
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-4527:
Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, focused refinement on core
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-4528:
Human post-catalytic spliceosome (P complex) stalled with DHX8 S717A mutant, focused refinement on core
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-4529:
Human post-catalytic spliceosome (P complex), focused refinement on Aquarius and SYF1
Method: EM (single particle) / Resolution: 5.9 Å

EMDB-4530:
Human post-catalytic spliceosome (P complex), focused refinement on Brr2
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-4532:
Human post-catalytic spliceosome (P complex), focused refinement on DHX8
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-4533:
Human post-catalytic spliceosome (P complex), focused refinement on Prp19
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-4534:
Human post-catalytic spliceosome (P complex), focused refinement on U2 snRNP
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-4535:
Human post-catalytic spliceosome (P complex), focused refinement on U5 Sm
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-ZN:
Unknown entry

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Source
  • homo sapiens (human)
  • human adenovirus 2
KeywordsSPLICING / spliceosome / RNA / complex

+
About EMN Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Oct 5, 2021. Nobel Prize for mechanically activated and temperature-gated ion channels

Nobel Prize for mechanically activated and temperature-gated ion channels

  • The Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian "for their discoveries of receptors for temperature and touch."
  • EM Navigator can help to find cryo-EM structure data by both pioneers.

External links:The Nobel Prize in Physiology or Medicine 2021 - NobelPrize.org / Structure data by Ardem Patapoutian / Structure data by David Julius

+
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jul 5, 2019. Downlodablable text data

Downlodablable text data

Some data of EM Navigator services can be downloaded as text file. Software such as Excel can load the data files.

PageDataFormat
EMN Searchsearch resultCSV, TSV, or JSON
EMN statisticsdata tableCSV or TSV

Related info.:EMN Search / EMN Statistics

-
EMN Papers

Database of articles cited by 3DEM data entries

  • Database of articles cited by 3DEM data entries in EMDB and PDB
  • Using PubMed data

Related info.:EMDB / PDB / Q: What are the data sources of EM Navigator? / EM Navigator / Yorodumi Papers / Changes in new EM Navigator and Yorodumi

Read more