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TitleSupercharging enables organized assembly of synthetic biomolecules.
Journal, issue, pagesNat Chem, Vol. 11, Issue 3, Page 204-212, Year 2019
Publish dateJan 14, 2019
AuthorsAnna J Simon / Yi Zhou / Vyas Ramasubramani / Jens Glaser / Arti Pothukuchy / Jimmy Gollihar / Jillian C Gerberich / Janelle C Leggere / Barrett R Morrow / Cheulhee Jung / Sharon C Glotzer / David W Taylor / Andrew D Ellington /
PubMed AbstractSymmetrical protein oligomers are ubiquitous in biological systems and perform key structural and regulatory functions. However, there are few methods for constructing such oligomers. Here we have ...Symmetrical protein oligomers are ubiquitous in biological systems and perform key structural and regulatory functions. However, there are few methods for constructing such oligomers. Here we have engineered completely synthetic, symmetrical oligomers by combining pairs of oppositely supercharged variants of a normally monomeric model protein through a strategy we term 'supercharged protein assembly' (SuPrA). We show that supercharged variants of green fluorescent protein can assemble into a variety of architectures including a well-defined symmetrical 16-mer structure that we solved using cryo-electron microscopy at 3.47 Å resolution. The 16-mer is composed of two stacked rings of octamers, in which the octamers contain supercharged proteins of alternating charges, and interactions within and between the rings are mediated by a variety of specific electrostatic contacts. The ready assembly of this structure suggests that combining oppositely supercharged pairs of protein variants may provide broad opportunities for generating novel architectures via otherwise unprogrammed interactions.
External linksNat Chem / PubMed:30643229
MethodsEM (single particle)
Resolution3.47 Å
Structure data

9104

6mdr

EMDB-9104, PDB-6mdr:
Cryo-EM structure of the Ceru+32/GFP-17 protomer
Method: EM (single particle) / Resolution: 3.47 Å

Source
  • aequorea victoria (jellyfish)
KeywordsLUMINESCENT PROTEIN / Supercharged protein assembly / electrostatic interactions / 16-mer / D4 symmetry

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