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| Title | Suppressor mutations in Rpf2-Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation. |
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| Journal, issue, pages | Nat Commun, Vol. 9, Issue 1, Page 4094, Year 2018 |
| Publish date | Oct 5, 2018 |
 Authors | Matthias Thoms / Valentin Mitterer / Lukas Kater / Laurent Falquet / Roland Beckmann / Dieter Kressler / Ed Hurt /   |
| PubMed Abstract | During eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in ...During eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in which a gently undulating α-helix corresponding to Cgr1 becomes wedged between Rsa4 and the relocated 5S RNP, but the purpose of this insertion was unknown. Here, we show that cgr1 deletion in yeast causes a slow-growth phenotype and reversion of the pre-60S particle to the pre-rotation stage. However, spontaneous extragenic suppressors could be isolated, which restore growth and pre-60S biogenesis in the absence of Cgr1. Whole-genome sequencing reveals that the suppressor mutations map in the Rpf2-Rrs1 module and Rpl5, which together stabilize the unrotated stage of the 5S RNP. Thus, mutations in factors stabilizing the pre-rotation stage facilitate 5S RNP relocation upon deletion of Cgr1, but Cgr1 itself could stabilize the post-rotation stage. |
 External links | Nat Commun / PubMed:30291245 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 11.0 - 16.0 Å |
| Structure data |  EMDB-0218:  EMDB-0219:  EMDB-0220:  EMDB-0221:  EMDB-0222:  EMDB-0223:  EMDB-0224: |
| Source |
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Saccharomyces cerevisiae (brewer's yeast)
