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TitleCryo-EM structures of fungal and metazoan mitochondrial calcium uniporters.
Journal, issue, pagesNature, Vol. 559, Issue 7715, Page 580-584, Year 2018
Publish dateJul 11, 2018
AuthorsRozbeh Baradaran / Chongyuan Wang / Andrew Francis Siliciano / Stephen Barstow Long /
PubMed AbstractThe mitochondrial calcium uniporter (MCU) is a highly selective calcium channel and a major route of calcium entry into mitochondria. How the channel catalyses ion permeation and achieves ...The mitochondrial calcium uniporter (MCU) is a highly selective calcium channel and a major route of calcium entry into mitochondria. How the channel catalyses ion permeation and achieves ion selectivity are not well understood, partly because MCU is thought to have a distinct architecture in comparison to other cellular channels. Here we report cryo-electron microscopy reconstructions of MCU channels from zebrafish and Cyphellophora europaea at 8.5 Å and 3.2 Å resolutions, respectively. In contrast to a previous report of pentameric stoichiometry for MCU, both channels are tetramers. The atomic model of C. europaea MCU shows that a conserved WDXXEP signature sequence forms the selectivity filter, in which calcium ions are arranged in single file. Coiled-coil legs connect the pore to N-terminal domains in the mitochondrial matrix. In C. europaea MCU, the N-terminal domains assemble as a dimer of dimers; in zebrafish MCU, they form an asymmetric crescent. The structures define principles that underlie ion permeation and calcium selectivity in this unusual channel.
External linksNature / PubMed:29995857 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 8.5 Å
Structure data

7971

6dnf

EMDB-7971: cryo-EM structure of the mitochondrial calcium uniporter MCU from the fungus Cyphellophora europaea
PDB-6dnf: Cryo-EM structure of the mitochondrial calcium uniporter MCU from the fungus Cyphellophora europaea
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-7972:
cryo-EM reconstruction of the mitochondrial calcium uniporter (MCU) from Zebrafish
Method: EM (single particle) / Resolution: 8.5 Å

Chemicals

ChemComp-DGG:
1-[GLYCEROLYLPHOSPHONYL]-2-[8-(2-HEXYL-CYCLOPROPYL)-OCTANAL-1-YL]-3-[HEXADECANAL-1-YL]-GLYCEROL

ChemComp-CA:
Unknown entry

Source
  • Cyphellophora europaea (fungus)
  • cyphellophora europaea cbs 101466 (fungus)
  • Danio rerio (zebrafish)
KeywordsMEMBRANE PROTEIN / mitochondria / calcium / ion channel / eukaryotic

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