[English] 日本語
EMN Papers
- Database of articles cited by 3DEM data entries -

+
Search query

Keywords
Author
Journal
IF

-
Structure paper

TitleVisualizing late states of human 40S ribosomal subunit maturation.
Journal, issue, pagesNature, Vol. 558, Issue 7709, Page 249-253, Year 2018
Publish dateJun 6, 2018
AuthorsMichael Ameismeier / Jingdong Cheng / Otto Berninghausen / Roland Beckmann /
PubMed AbstractThe formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high- ...The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage.
External linksNature / PubMed:29875412
MethodsEM (single particle)
Resolution3.5 - 4.5 Å
Structure data

4337

6g18

EMDB-4337, PDB-6g18:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State C
Method: EM (single particle) / Resolution: 3.6 Å

4348

6g4s

EMDB-4348, PDB-6g4s:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State B
Method: EM (single particle) / Resolution: 4.0 Å

4349

6g4w

EMDB-4349, PDB-6g4w:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State A
Method: EM (single particle) / Resolution: 4.5 Å

4350

6g51

EMDB-4350, PDB-6g51:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State D
Method: EM (single particle) / Resolution: 4.1 Å

4351

6g53

EMDB-4351, PDB-6g53:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State E
Method: EM (single particle) / Resolution: 4.5 Å

4352

6g5h

EMDB-4352, PDB-6g5h:
Cryo-EM structure of a late human pre-40S ribosomal subunit - Mature
Method: EM (single particle) / Resolution: 3.6 Å

4353

6g5i

EMDB-4353, PDB-6g5i:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State R
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • Human (human)
KeywordsRIBOSOME / 40S / pre-40S / ribosome biogenesis

+
About EMN Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Oct 5, 2021. Nobel Prize for mechanically activated and temperature-gated ion channels

Nobel Prize for mechanically activated and temperature-gated ion channels

  • The Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian "for their discoveries of receptors for temperature and touch."
  • EM Navigator can help to find cryo-EM structure data by both pioneers.

External links:The Nobel Prize in Physiology or Medicine 2021 - NobelPrize.org / Structure data by Ardem Patapoutian / Structure data by David Julius

+
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jul 5, 2019. Downlodablable text data

Downlodablable text data

Some data of EM Navigator services can be downloaded as text file. Software such as Excel can load the data files.

PageDataFormat
EMN Searchsearch resultCSV, TSV, or JSON
EMN statisticsdata tableCSV or TSV

Related info.:EMN Search / EMN Statistics

-
EMN Papers

Database of articles cited by 3DEM data entries

  • Database of articles cited by 3DEM data entries in EMDB and PDB
  • Using PubMed data

Related info.:EMDB / PDB / Q: What are the data sources of EM Navigator? / EM Navigator / Yorodumi Papers / Changes in new EM Navigator and Yorodumi

Read more