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Structure paper

TitleStructure and Dynamics of a 197 bp Nucleosome in Complex with Linker Histone H1.
Journal, issue, pagesMol Cell, Vol. 66, Issue 3, Page 384-397.e8, Year 2017
Publish dateMay 4, 2017
AuthorsJan Bednar / Isabel Garcia-Saez / Ramachandran Boopathi / Amber R Cutter / Gabor Papai / Anna Reymer / Sajad H Syed / Imtiaz Nisar Lone / Ognyan Tonchev / Corinne Crucifix / Hervé Menoni / Christophe Papin / Dimitrios A Skoufias / Hitoshi Kurumizaka / Richard Lavery / Ali Hamiche / Jeffrey J Hayes / Patrick Schultz / Dimitar Angelov / Carlo Petosa / Stefan Dimitrov /
PubMed AbstractLinker histones associate with nucleosomes to promote the formation of higher-order chromatin structure, but the underlying molecular details are unclear. We investigated the structure of a 197 bp ...Linker histones associate with nucleosomes to promote the formation of higher-order chromatin structure, but the underlying molecular details are unclear. We investigated the structure of a 197 bp nucleosome bearing symmetric 25 bp linker DNA arms in complex with vertebrate linker histone H1. We determined electron cryo-microscopy (cryo-EM) and crystal structures of unbound and H1-bound nucleosomes and validated these structures by site-directed protein cross-linking and hydroxyl radical footprinting experiments. Histone H1 shifts the conformational landscape of the nucleosome by drawing the two linkers together and reducing their flexibility. The H1 C-terminal domain (CTD) localizes primarily to a single linker, while the H1 globular domain contacts the nucleosome dyad and both linkers, associating more closely with the CTD-distal linker. These findings reveal that H1 imparts a strong degree of asymmetry to the nucleosome, which is likely to influence the assembly and architecture of higher-order structures.
External linksMol Cell / PubMed:28475873 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution5.4 - 11.4 Å
Structure data

EMDB-3657:
Nucleosome in complex with human histone H1.5dC50
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-3659:
601 nucleosome in complex with X. laevis histone H1.0b
Method: EM (single particle) / Resolution: 11.4 Å

EMDB-3660:
601 nucleosome
Method: EM (single particle) / Resolution: 11.4 Å

PDB-5nl0:
Crystal structure of a 197-bp palindromic 601L nucleosome in complex with linker histone H1
Method: X-RAY DIFFRACTION / Resolution: 5.4 Å

Source
  • Homo sapiens (human)
  • xenopus laevis (African clawed frog)
  • synthetic construct (others)
KeywordsCHROMATIN BINDING PROTEIN / DNA / nucleosome / chromatin / linker histones / histone H1 / CHROMATIN BINDING PROTEIN - DNA complex

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