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-Structure paper
| Title | Architecture of a transcribing-translating expressome. |
|---|---|
| Journal, issue, pages | Science, Vol. 356, Issue 6334, Page 194-197, Year 2017 |
| Publish date | Apr 14, 2017 |
 Authors | R Kohler / R A Mooney / D J Mills / R Landick / P Cramer /   |
| PubMed Abstract | DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of can ...DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of can form a defined transcribing and translating "expressome" complex. The cryo-electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit α carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination. |
 External links | Science / PubMed:28408604 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 6.4 - 7.6 Å |
| Structure data |  EMDB-3579: |
| Chemicals |  ChemComp-ZN: |
| Source |
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 Keywords |  RIBOSOME / expressome ribosome RNA polymerase |
