EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM Structure of the relaxosome, a complex essential for bacterial mating and the spread of antibiotic resistance genes.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 4906, Year 2025
掲載日	2025年5月27日
著者	Sunanda M Williams / Sandra Raffl / Sabine Kienesberger / Aravindan Ilangovan / Ellen L Zechner / Gabriel Waksman /
PubMed 要旨	Bacterial mating, or conjugation, was discovered nearly 80 years ago as a process transferring genes from one bacterial cell (the donor) to another (the recipient). It requires three key multiprotein ...Bacterial mating, or conjugation, was discovered nearly 80 years ago as a process transferring genes from one bacterial cell (the donor) to another (the recipient). It requires three key multiprotein complexes in the donor cell: a DNA-processing machinery called the relaxosome, a double-membrane spanning type 4 secretion system (T4SS), and an extracellular appendage termed pilus. While the near-atomic resolution structures of the T4SS and pilus are already known, that of the relaxosome has not been reported to date. Here, we describe the cryo-EM structure of the fully assembled relaxosome encoded by the paradigm F plasmid in two different states corresponding to distinct functional steps along the DNA processing reaction. By varying the structures of model DNAs we delineate conformational changes required to initiate conjugation. Mutational studies of the various protein-protein and protein-DNA interaction hubs suggest a complex sensitive to trigger signals, that could arise from cell-to-cell contacts with recipient cells.
リンク	Nat Commun / PubMed:40425557 / PubMed Central
手法	EM (単粒子)
解像度	2.94 - 7.7 Å
構造データ	EMDB-50098: Initial 3D Map of relaxosome complex with oriT DNA ds-27_+143 手法: EM (単粒子) / 解像度: 6.34 Å EMDB-50099: Initial 3D Map of relaxosome complex with oriT DNA ss-27_+8ds+9_+143 手法: EM (単粒子) / 解像度: 3.81 Å EMDB-50102: Initial 3D Map of relaxosome complex with oriT DNA ss-27_-8ds-7_+143 手法: EM (単粒子) / 解像度: 7.7 Å EMDB-50103: Initial 3D Map of relaxosome complex with oriT DNA ss-27_-13ds-12_+143 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-50104: Initial 3D Map of relaxosome complex with oriT DNA ds-2_+113deltaTraM 手法: EM (単粒子) / 解像度: 6.14 Å EMDB-50105: Initial 3D Map of relaxosome complex with oriT DNA ds-67_+113(poly-dT15-17_-3)deltaTraM 手法: EM (単粒子) / 解像度: 4.43 Å 50117 9f0x EMDB-50117: CryoEM map of the F plasmid relaxosome in its pre-initiation state. ds-27_+143-R Locally-refined Map 3.76 A PDB-9f0x: CryoEM structure of the F plasmid relaxosome in its pre-initiation state, derived from the ds-27_+143-R Locally-refined Map 3.76 A 手法: EM (単粒子) / 解像度: 3.78 Å 50118 9f0y EMDB-50118, PDB-9f0y: CryoEM map of the F plasmid relaxosome with TraI in its TE mode. ss-27_+8ds+9_+143-R Locally-refined 3.45 A Map 手法: EM (単粒子) / 解像度: 3.45 Å 50119 9f0z EMDB-50119: CryoEM map of the F plasmid relaxosome with truncated TraI1-863 in its TE mode. ss-27_+8ds+9_+143-R_deltaAH+CTD Locally-refined 3.42 A Map PDB-9f0z: CryoEM structure of the F plasmid relaxosome with truncated TraI1-863 in its TE mode, derived from the ss-27_+8ds+9_+143-R_deltaAH+CTD Locally-refined 3.42 A Map 手法: EM (単粒子) / 解像度: 3.42 Å 50120 9f10 EMDB-50120: CryoEM map of the F plasmid relaxosome with TraI in its TE mode, without accessory protein TraM. ss-27_+8ds+9_+143-R_deltaTraM Locally-refined 2.94 A Map. PDB-9f10: CryoEM structure of the F plasmid relaxosome with TraI in its TE mode, without accessory protein TraM. Derived from the ss-27_+8ds+9_+143-R_deltaTraM Locally-refined 2.94 A Map. 手法: EM (単粒子) / 解像度: 2.94 Å 50121 9f11 EMDB-50121: CryoEM map of the F plasmid relaxosome with oriT DNA ss-27_+3ds+4_+143 and TraI in its TE mode. ss-27_+3ds+4_+143-R Locally-refined 3.68 A Map. PDB-9f11: CryoEM structure of the F plasmid relaxosome with oriT DNA ss-27_+3ds+4_+143 and TraI its TE mode, derived from ss-27_+3ds+4_+143-R Locally-refined 3.68 A Map. 手法: EM (単粒子) / 解像度: 3.68 Å 50122 9f12 EMDB-50122: CryoEM map of the F plasmid relaxosome with oriT DNA ss-27_-3ds-2_+143 and TraI in its TE mode. ss-27_-3ds-2_+143-R Locally-refined 3.42 A Map. PDB-9f12: CryoEM structure of the F plasmid relaxosome with oriT DNA ss-27_-3ds-2_+143 and TraI its TE mode, derived from ss-27_-3ds-2_+143-R Locally-refined 3.42 A Map. 手法: EM (単粒子) / 解像度: 3.42 Å EMDB-50131: CryoEM map of the F plasmid relaxosome in its pre-initiation state. ds-27_+143-R Global Map 4.31 A. 手法: EM (単粒子) / 解像度: 4.31 Å EMDB-50132: CryoEM map of the F plasmid relaxosome with truncated TraI1-863 in its TE mode. ss-27_+8ds+9_+143-R_deltaAH+CTD Global 3.93 A Map. 手法: EM (単粒子) / 解像度: 3.93 Å EMDB-50133: CryoEM map of the F plasmid relaxosome with TraI in its TE mode, without the accessory protein TraM. ss-27_+8ds+9_+143-R_deltaTraM Global 3.11 A Map. 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-53548: CryoEM map of the F plasmid relaxosome with TraI in its TE mode. ss-27_+8ds+9_+143-R Global 3.77 A Map. 手法: EM (単粒子) / 解像度: 3.77 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	escherichia coli k-12 (大腸菌)
キーワード	DNA BINDING PROTEIN / Relaxosome / Bacterial Conjugation / DNA processing / Relaxase / DNA binding proteins