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- EMDB-42441: Major interface of Streptococcal surface enolase dimer from AP53 ... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Major interface of Streptococcal surface enolase dimer from AP53 group A streptococcus bound to a lipid vesicle | |||||||||
![]() | Minor interface of streptococcal surface enolase bound to a lipid main map | |||||||||
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![]() | Glycolysis / plasminogen binder / membrane protein / LYASE | |||||||||
Function / homology | ![]() phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / peptidoglycan-based cell wall / glycolytic process / magnesium ion binding / cell surface / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
![]() | Tjia-Fleck S / Readnour BM / Castellino FJ | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Streptococcus surface alpha enolase exposed dimers were found to be the active form on lipid surface that binds to human plasminogen Authors: Tjia-Fleck S / Readnour BM / Castellino FJ | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 35.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.3 KB 15.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 7 KB | Display | ![]() |
Images | ![]() | 105.8 KB | ||
Filedesc metadata | ![]() | 5.7 KB | ||
Others | ![]() ![]() | 34.6 MB 34.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8uoyMC ![]() 8uopC ![]() 8uq6C C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Minor interface of streptococcal surface enolase bound to a lipid main map | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.078 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Minor interface of streptococcal surface enolase bound to...
File | emd_42441_half_map_1.map | ||||||||||||
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Annotation | Minor interface of streptococcal surface enolase bound to a lipid half map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Minor interface of streptococcal surface enolase bound to...
File | emd_42441_half_map_2.map | ||||||||||||
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Annotation | Minor interface of streptococcal surface enolase bound to a lipid half map B | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Streptococcal surface enolase major interface embedded into the s...
Entire | Name: Streptococcal surface enolase major interface embedded into the surface of a lipid vesicle |
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Components |
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-Supramolecule #1: Streptococcal surface enolase major interface embedded into the s...
Supramolecule | Name: Streptococcal surface enolase major interface embedded into the surface of a lipid vesicle type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 95 KDa |
-Macromolecule #1: Enolase
Macromolecule | Name: Enolase / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: phosphopyruvate hydratase |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 47.543281 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: HMSIITDVYA REVLDSRGNP TLEVEVYTES GAFGRGMVPS GASTGEHEAV ELRDGDKSRY LGLGTQKAVD NVNNIIAEAI IGYDVRDQQ AIDRAMIALD GTPNKGKLGA NAILGVSIAV ARAAADYLEV PLYTYLGGFN TKVLPTPMMN IINGGSHSDA P IAFQEFMI ...String: HMSIITDVYA REVLDSRGNP TLEVEVYTES GAFGRGMVPS GASTGEHEAV ELRDGDKSRY LGLGTQKAVD NVNNIIAEAI IGYDVRDQQ AIDRAMIALD GTPNKGKLGA NAILGVSIAV ARAAADYLEV PLYTYLGGFN TKVLPTPMMN IINGGSHSDA P IAFQEFMI MPVGAPTFKE GLRWGAEVFH ALKKILKERG LVTAVGDEGG FAPKFEGTED GVETILKAIE AAGYEAGENG IM IGFDCAS SEFYDKERKV YDYTKFEGEG AAVRTSAEQV DYLEELVNKY PIITIEDGMD ENDWDGWKVL TERLGKRVQL VGD DFFVTN TEYLARGIKE NAANSILIKV NQIGTLTETF EAIEMAKEAG YTAVVSHRSG ETEDSTIADI AVATNAGQIK TGSL SRTDR IAKYNQLLRI EDQLGEVAQY KGIKSFYNLK K UniProtKB: Enolase |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 29.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |