+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-47001 | |||||||||
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Title | Streptoccocal alpha enolase embedded in DOPG lipid vesicle | |||||||||
Map data | Streptococcal alpha enolase on lipid DOPG vesicle | |||||||||
Sample |
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Keywords | Glycolysis / plasminogen binder / membrane protein / LYASE | |||||||||
Biological species | Streptococcus pyogenes (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.0 Å | |||||||||
Authors | Tjia-Fleck S / Readnour BM / Castellino FJ | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Streptococcus surface alpha enolase exposed dimers were found to be the active form on lipid surface that binds to human plasminogen Authors: Tjia-Fleck S / Readnour BM / Castellino FJ | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_47001.map.gz | 55.9 MB | EMDB map data format | |
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Header (meta data) | emd-47001-v30.xml emd-47001.xml | 9.7 KB 9.7 KB | Display Display | EMDB header |
Images | emd_47001.png | 132.7 KB | ||
Filedesc metadata | emd-47001.cif.gz | 4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-47001 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-47001 | HTTPS FTP |
-Validation report
Summary document | emd_47001_validation.pdf.gz | 561.2 KB | Display | EMDB validaton report |
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Full document | emd_47001_full_validation.pdf.gz | 560.8 KB | Display | |
Data in XML | emd_47001_validation.xml.gz | 6.4 KB | Display | |
Data in CIF | emd_47001_validation.cif.gz | 7.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-47001 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-47001 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_47001.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Streptococcal alpha enolase on lipid DOPG vesicle | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.29052 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Streptococcal surface enolase major interface embedded into the s...
Entire | Name: Streptococcal surface enolase major interface embedded into the surface of a lipid vesicle |
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Components |
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-Supramolecule #1: Streptococcal surface enolase major interface embedded into the s...
Supramolecule | Name: Streptococcal surface enolase major interface embedded into the surface of a lipid vesicle type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Streptococcus pyogenes (bacteria) |
Molecular weight | Theoretical: 95 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 29.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |