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- EMDB-41284: Combined linker domain of N-DRC and associated proteins Tetrahymena -

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Basic information

Entry
Database: EMDB / ID: EMD-41284
TitleCombined linker domain of N-DRC and associated proteins Tetrahymena
Map dataMain map
Sample
  • Cell: Combined linker domain of N-DRC and associated proteins Tetrahymena
    • Protein or peptide: x 24 types
Keywordsnexin-dynein regulatory complex / cilia / axoneme / dynein / STRUCTURAL PROTEIN
Function / homology
Function and homology information


axonemal dynein complex / axonemal dynein complex assembly / inner dynein arm assembly / cilium movement / axoneme assembly / motile cilium / axoneme / cell projection / cell motility / cilium ...axonemal dynein complex / axonemal dynein complex assembly / inner dynein arm assembly / cilium movement / axoneme assembly / motile cilium / axoneme / cell projection / cell motility / cilium / small GTPase binding / microtubule binding / microtubule / cytoskeleton / calcium ion binding / ATP hydrolysis activity / ATP binding / cytoplasm
Similarity search - Function
Coiled-coil domain-containing protein 153 / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / Dynein regulatory complex protein 9 / Cilia- and flagella-associated protein 91 / Growth arrest-specific protein 8 domain ...Coiled-coil domain-containing protein 153 / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / Dynein regulatory complex protein 9 / Cilia- and flagella-associated protein 91 / Growth arrest-specific protein 8 domain / Growth arrest-specific protein 8 / Dynein regulatory complex protein 1/2, N-terminal / Dynein regulatory complex protein / Growth-arrest specific micro-tubule binding / Sperm tail / BRE1 E3 ubiquitin ligase / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / EF-hand domain / Leucine rich repeat, ribonuclease inhibitor type / Leucine rich repeat 4 / Leucine Rich repeats (2 copies) / Leucine Rich repeat / Leucine-rich repeat, SDS22-like subfamily / IQ motif profile. / IQ motif, EF-hand binding site / Leucine-rich repeat profile. / Leucine-rich repeat / Papain-like cysteine peptidase superfamily / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / Leucine-rich repeat domain superfamily / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Dynein regulatory complex protein 10 / Growth-arrest-specific microtubule-binding protein / AAA family ATPase CDC48 subfamily protein / Cilia- and flagella-associated protein 91 / Cilia- and flagella-associated protein 184 / Calmodulin 7-2 / Dynein regulatory complex subunit 3 / Coiled-coil lobo-like protein, putative / EF hand protein / Dynein regulatory complex protein 1/2 N-terminal domain-containing protein ...Dynein regulatory complex protein 10 / Growth-arrest-specific microtubule-binding protein / AAA family ATPase CDC48 subfamily protein / Cilia- and flagella-associated protein 91 / Cilia- and flagella-associated protein 184 / Calmodulin 7-2 / Dynein regulatory complex subunit 3 / Coiled-coil lobo-like protein, putative / EF hand protein / Dynein regulatory complex protein 1/2 N-terminal domain-containing protein / Cilia- and flagella-associated protein 263 / Cilia- and flagella-associated protein 20 / Coiled-coil domain-containing protein 153 / Coiled-coil protein, putative / WD40 domain protein / Flagella associated protein / Dynein regulatory complex protein 9 / Growth-arrest-specific microtubule-binding protein / Flagellar associated protein / Kinase domain protein / Dynein regulatory complex subunit 2 / WD40 domain protein / EF-hand calcium-binding domain protein
Similarity search - Component
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsGhanaeian AG / Majhi SM / McCaffrey CM / Nami BN / Black CB / Yang SK / Legal TL / Papoulas OP / Janowska MJ / Valente-Paterno MV ...Ghanaeian AG / Majhi SM / McCaffrey CM / Nami BN / Black CB / Yang SK / Legal TL / Papoulas OP / Janowska MJ / Valente-Paterno MV / Marcotte EM / Wloga DW / Bui KH
Funding support Canada, 2 items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR) Canada
Natural Sciences and Engineering Research Council (NSERC, Canada) Canada
CitationJournal: Nat Commun / Year: 2023
Title: Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism.
Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward ...Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui /
Abstract: Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein ...Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity.
History
DepositionJul 19, 2023-
Header (metadata) releaseSep 27, 2023-
Map releaseSep 27, 2023-
UpdateApr 3, 2024-
Current statusApr 3, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_41284.png

Downloads & links

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Map

FileDownload / File: emd_41284.map.gz / Format: CCP4 / Size: 401.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMain map
Voxel sizeX=Y=Z: 2.74 Å
Density
Contour LevelBy AUTHOR: 0.545
Minimum - Maximum-1.58187 - 3.4972267
Average (Standard dev.)0.0069336235 (±0.2399834)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-1-1-1
Dimensions472472472
Spacing472472472
CellA=B=C: 1293.28 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_41284_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: Half map

Fileemd_41284_half_map_1.map
AnnotationHalf map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map

Fileemd_41284_half_map_2.map
AnnotationHalf map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : Combined linker domain of N-DRC and associated proteins Tetrahymena

EntireName: Combined linker domain of N-DRC and associated proteins Tetrahymena
Components
  • Cell: Combined linker domain of N-DRC and associated proteins Tetrahymena
    • Protein or peptide: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
    • Protein or peptide: Coiled-coil protein, putativeCoiled coil
    • Protein or peptide: LRRC48 protein
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Flagellar associated protein
    • Protein or peptide: Kinase domain protein
    • Protein or peptide: Coiled-coil lobo-like protein, putative
    • Protein or peptide: EF-hand calcium-binding domain protein
    • Protein or peptide: Dynein regulatory complex protein 9
    • Protein or peptide: Dynein regulatory complex protein 10
    • Protein or peptide: AAA family ATPase CDC48 subfamily protein
    • Protein or peptide: Cilia- and flagella-associated protein 91
    • Protein or peptide: Flagella associated protein
    • Protein or peptide: Coiled-coil protein, putativeCoiled coil
    • Protein or peptide: DUF4201 domain-containing protein
    • Protein or peptide: Calmodulin 7-2
    • Protein or peptide: Coiled-coil domain-containing protein 153
    • Protein or peptide: Flagellar associated protein
    • Protein or peptide: L-shape (Unknown-protein)
    • Protein or peptide: WD40 domain protein
    • Protein or peptide: EF hand protein
    • Protein or peptide: CFAP20
    • Protein or peptide: WD40 domain protein

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Supramolecule #1: Combined linker domain of N-DRC and associated proteins Tetrahymena

SupramoleculeName: Combined linker domain of N-DRC and associated proteins Tetrahymena
type: cell / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Tetrahymena thermophila (eukaryote)

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Macromolecule #1: Dynein regulatory complex protein 1/2 N-terminal domain-containin...

MacromoleculeName: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 98.840125 KDa
SequenceString: MNPSQTQKGK IQIQVSRGSI DLLVRTMYED INNIKLKNER FQKKKRAEDE RRRRERYAEI QQEAIASGKK NAALEMKWAE LKELDECEE LNREMQDQQQ TFQQIIEGKE NLRKEFEEEL KRKDDEYVKM LKEQSNDIKD MISKMRSQFY KIRDANMQEL E DIERHFEQ ...String:
MNPSQTQKGK IQIQVSRGSI DLLVRTMYED INNIKLKNER FQKKKRAEDE RRRRERYAEI QQEAIASGKK NAALEMKWAE LKELDECEE LNREMQDQQQ TFQQIIEGKE NLRKEFEEEL KRKDDEYVKM LKEQSNDIKD MISKMRSQFY KIRDANMQEL E DIERHFEQ ERSNLLNEFK AKIQATFKKH LDLEEQYVKE HAKTEEEQTK NIEDLRIKGA KHYAELKITM ETEIQDLEKC FE DMKALYQ LITEKLDYSL KVLQEKFHEN NSMCDELKRK ENNFKNRLKQ LTKDYKQYDK KFKLENKNLT QEYKRIIRQF KEL QKKFKH FEKADLDKYS EIQKMNEAEV KELKDKIIKC NITIHIQQLG MQWIPPQSEE EILAQQKLQL EQGGKAEEEE EREF EFCIS EIKIGEICNI ILEEADFLID DKLREELEGS AEPEQIFQRL DCIKKCLYID SEDEMNLFFR ELITKCRVKS AEEIE EEAR KQLLLLGLIK EAEENENPEG EEVQQQEGAQ QKKKEEGNKK DDPKGKEGAK DSKENANPQG QNKSNQDERK KKKDGQ DDH AGQGQGQYDS NQGGMGENQE EGENAEGQEN LEGEIEKEID LENTQINLNE VVKFLQNWQA NKDKRKEKLE KESSKKA VK QETEREKKER IAREGKKYWE KLTQVLPERT FRIWNVNFFK FSFEKQILLN QINKLRFWTD LCPNIMNFFW TDKNQSKK P VNCITKMKNQ RIFQINTYKL IMNLLSALQD SLIQIKDKDD FIQTKISTNL FTFHNFQILL QIFIVNQSFL FIRLCQSYS KKIILLIKNI NQKLFNLSNF KNQFSF

UniProtKB: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein

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Macromolecule #2: Coiled-coil protein, putative

MacromoleculeName: Coiled-coil protein, putative / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 60.596348 KDa
SequenceString: MALLGKTLKG RSTRPGGIPQ KRNIKWRQLA KNQEEFDQLK QLAKMKREGL KARIKDEQKV VTFNKKKLIT YWRKIMRIAK TEQLKNEID IYSQNNQREL DSKEAFIQML DKNLDEAEDQ FQIALRNHLI HIENLMQLQE ARMRGLAEEF NRDVNILETE F DLEREEMV ...String:
MALLGKTLKG RSTRPGGIPQ KRNIKWRQLA KNQEEFDQLK QLAKMKREGL KARIKDEQKV VTFNKKKLIT YWRKIMRIAK TEQLKNEID IYSQNNQREL DSKEAFIQML DKNLDEAEDQ FQIALRNHLI HIENLMQLQE ARMRGLAEEF NRDVNILETE F DLEREEMV KTHKTQLKEL EDMIETVKEE DKKKTEEAQN EFSQFKEETK NKNLEETNVM KIILETKQTK YYTELEQMNS KF QSDTSNK VKDHQFYHAH NKNRKQEIDR YLRTISSKKA KIDLMKLKIL QHCKEFNARN SALKKEKENI SRNYHELKLK MQK FREEES RRLKELSNNS RNAVLKLREY CALGEKILKT AELCRRLETE KEKVLPFYES SVDEDQIPEQ LKNEFEHLKK EDAE EYAYL NNFYKRYNKV LLDKLAIEKQ KENLQRDNQL LKSLLKQYLD GISLNDDVLK NENNPLLVVN HKFNLGKMPV EKIEN KTVI EGVFEVRNTS HQLQGQRAPP FQ

UniProtKB: Dynein regulatory complex subunit 2

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Macromolecule #3: LRRC48 protein

MacromoleculeName: LRRC48 protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 68.009617 KDa
SequenceString: MSNYISNYVQ STIVTKTVQN KDKKTGKNSV DPRVINEQLI LDAVKQFNQE NNKITTENII LQQLRQLQLS FKNILKIQHL QGLERLEKL QLDNNIIEKI ENIDHLVNLK WLDLSFNCIK KIEGLDKLKE LTDLSLFNNY IEKIEGLHNN TKLNVFSIGN N RLKSYEEI ...String:
MSNYISNYVQ STIVTKTVQN KDKKTGKNSV DPRVINEQLI LDAVKQFNQE NNKITTENII LQQLRQLQLS FKNILKIQHL QGLERLEKL QLDNNIIEKI ENIDHLVNLK WLDLSFNCIK KIEGLDKLKE LTDLSLFNNY IEKIEGLHNN TKLNVFSIGN N RLKSYEEI TLYFGYKPRG EEGTNDRPEF KRLQVLNVSG NPFTKDKENE YKNHIICAIP NLKYLDYVFI DEGDRQLIRQ DE QISNSYN FTTTDYYQQM QAQEQKEEMD RQTLKKKKDA RMDILDNLKD EYLKIEDLQR VKIIKPNQIN EEIEKYCGKI NDH VVEMQA AVIQKHQLIL QEMSKTEVSY KEQEALHEKE TLQILKEFEH EKKVEFRNWE KEAANGDAKK EESRLKTLLQ KTTS LKNKL MDIEIQLVEE LEAIFTDYDT KKKETAWKDI EEIIVLGQKK IEEENVKFYS NLNVIKNKEE ANYNSTQQQQ LEEEN NEED DEKASLLENK EQLAQMIGNI KETLIDSHTK IILSAQMELQ KDLAEYAKQQ KNKMYERNRK NISQIIAQID AYQTEI NEK LKQGDDESDH EN

UniProtKB: Dynein regulatory complex subunit 3

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Macromolecule #4: Growth-arrest-specific microtubule-binding protein

MacromoleculeName: Growth-arrest-specific microtubule-binding protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 56.190844 KDa
SequenceString: MSKAAKAKKN VPVVSKLEAD ARKAAEGVND NESEEFKKAM RKEARALVEQ FNEEKKLLAF YQQERQKINY NWIIAKKELE DKKSELINK EREIQDLQEN HFMTLNVYKQ KIKHLLFQNQ DQQSELKKDV EVTLKQLEDQ HRIKSRELKT DVRSLKVTKK E QEISQQDY ...String:
MSKAAKAKKN VPVVSKLEAD ARKAAEGVND NESEEFKKAM RKEARALVEQ FNEEKKLLAF YQQERQKINY NWIIAKKELE DKKSELINK EREIQDLQEN HFMTLNVYKQ KIKHLLFQNQ DQQSELKKDV EVTLKQLEDQ HRIKSRELKT DVRSLKVTKK E QEISQQDY LFALKSEHDK QMTLMRQDYE RQVNDIKRKY DLKMQNLTKE MEEARAAMIK QLEDNKNQKI AEIIKEHTQK YN DIKNYYS EITATNLDYR KTLKNEIKEL QTKDEEYKKT LQQTEKGYKE LNEPLQALGI EIIQLKKQDE EQEAIIKEKE ELK QKIDNQ ERLFRKLEYE YEVKLQQFQY LERERNALYA KFNQTVFEIH QKSGLENLIL EKKVTNLRED LEIKDLQIHQ VLTA ANIDP NSVGSINKSL EEVESLKNEL ISELQAQLKQ IRKAHSHMVK AYEGKLSEFV IPVEELGFDP LVPTNTD

UniProtKB: Growth-arrest-specific microtubule-binding protein

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Macromolecule #5: Growth-arrest-specific microtubule-binding protein

MacromoleculeName: Growth-arrest-specific microtubule-binding protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 55.393203 KDa
SequenceString: MPPKKAKGKK KKEEEPDDEY KSMTGADLTQ TLEKLKERVN EMRTNRNYIQ MDRDMVENFY HNTLKEISEV KTKISNKETE AEEKESKHR IDVKVFLQKV KHLEYEQEKS NLNIEDDGKK AKEKEDAYFE DITKNMKQLK TQLKSEYLEK EKANIQQVQE E KKDHQSLL ...String:
MPPKKAKGKK KKEEEPDDEY KSMTGADLTQ TLEKLKERVN EMRTNRNYIQ MDRDMVENFY HNTLKEISEV KTKISNKETE AEEKESKHR IDVKVFLQKV KHLEYEQEKS NLNIEDDGKK AKEKEDAYFE DITKNMKQLK TQLKSEYLEK EKANIQQVQE E KKDHQSLL KIQQKKFDEL INNLIIKYEE RLAKLKEDLE LKLKVEIHEL EERKNLHINE LMNNHEKAFA ELKKYYNDIT AE NLNLIKA HKEKIAQIYA NIQLNTKNVA DNQAKNEQLK EPLAKHREIR NKLKEDLKQF AKHKMSLQNL KSKAITLKDK ITK LERDGK DLDEKYEKVV REKQELEKKF EDITQEVKKN ADLNNNVLSN RLQILLKEYN NKEEELRTII DNAGLDHNLH EQLK QRVQQ SIEAKNTLIK NLKYSIHHAT KAYNDAIRVY EAKLVEFGIP IEELGFQPLE TITSSMPAGL VSS

UniProtKB: Growth-arrest-specific microtubule-binding protein

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Macromolecule #6: Flagellar associated protein

MacromoleculeName: Flagellar associated protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 52.938457 KDa
SequenceString: MLAKKLALAR SQGKPDPFKK DEFPLLQHIA VEVVSQNYIL YPDLKGVPEN VRDTIISKIT TDLDILTMAP NIDQESFWQR ACKNRWEKS QKSINIEEHG RSWKVAYLER YMEEFLTNIQ NADDPNKKQI LQAELKAAAS WIHTLNLQNI NQNIDIDFIC K YLPLLTSL ...String:
MLAKKLALAR SQGKPDPFKK DEFPLLQHIA VEVVSQNYIL YPDLKGVPEN VRDTIISKIT TDLDILTMAP NIDQESFWQR ACKNRWEKS QKSINIEEHG RSWKVAYLER YMEEFLTNIQ NADDPNKKQI LQAELKAAAS WIHTLNLQNI NQNIDIDFIC K YLPLLTSL TLTYGTKYSG MDYNKQSVGM KLSEAANLGE AIKNCYSLLS LNISANMIDD DLLRFIMAGV NMNISLIELN LS HNKIEDQ GARRIAKFLM RNEILLYLNL GNNLIGYEGS RYLAQALKVN KNLQSLNLKL NNLGDKAGKK LFQDLMLNKT LLE LDVSGN QFEFETALKL SDYVADSMCG LKVLNIANND FNDSCYENLK TGFTKNYSLA KLDLRGNKFS KTQEEKEQEL KDPF RMFNL SQTEKELTQI IIRHDLTAQK IQFFSESDLD KIKQLKELQG AKQEPEKVIQ QDQNKE

UniProtKB: Flagellar associated protein

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Macromolecule #7: Kinase domain protein

MacromoleculeName: Kinase domain protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 39.706977 KDa
SequenceString: MGDKKGKKKG GGEEDESTLQ LSRQYKKKCE LNGINPSKLF KEKLDFAVEE DENIEKIHLW EELGPVGVRS IMDSLTEIPY LHTKSIRLW KVKAQDEGTR TICNYMEKSK TIEYLDLMDN QVGSLGCEFL GRVLHPQIEI PLLKLKLDHN EFGTEGLMQL A AGLCMNSV ...String:
MGDKKGKKKG GGEEDESTLQ LSRQYKKKCE LNGINPSKLF KEKLDFAVEE DENIEKIHLW EELGPVGVRS IMDSLTEIPY LHTKSIRLW KVKAQDEGTR TICNYMEKSK TIEYLDLMDN QVGSLGCEFL GRVLHPQIEI PLLKLKLDHN EFGTEGLMQL A AGLCMNSV LEKLSLNYCA ITAEGSKYIQ QILSFVNTNL KKLKMKGNLL KNEGVHQLFR AFKVNKTLEK VDLSDNQFDT TL GDDQDPE ANPNFFETNK DAIEHNKQII ENICEVLKAA DTLLYYDFRF NNISKTDAKA IIDCLEANKN IYYMELSEHI PKD LIERKK GLMKKRKPKK KKKKKAKKK

UniProtKB: Kinase domain protein

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Macromolecule #8: Coiled-coil lobo-like protein, putative

MacromoleculeName: Coiled-coil lobo-like protein, putative / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 101.225328 KDa
SequenceString: MDHNEGGYSH HQIEGQEGQQ QQAPVGGGGE DIFQVLENIT QVQNEKLKRC QEFKEQENIP RQYIENSPKE ELVLEHVIQF KRQFQYQLY YEDKRDLFLY PKNECDVYKF ICTTIRPTKL GFLELYDYQQ CSKYLSEFVQ YEELDPPNEF PLVIPSPTNV A VWQKGDCF ...String:
MDHNEGGYSH HQIEGQEGQQ QQAPVGGGGE DIFQVLENIT QVQNEKLKRC QEFKEQENIP RQYIENSPKE ELVLEHVIQF KRQFQYQLY YEDKRDLFLY PKNECDVYKF ICTTIRPTKL GFLELYDYQQ CSKYLSEFVQ YEELDPPNEF PLVIPSPTNV A VWQKGDCF DMSILLCSLL IGVGYDAYCV YGKAPREITT RNESLMECLF LEKGKYIEEN DKKPKQTLEQ NENALLKKPI AH SKWDQTQ ETKAREAEEE KRRIALTIND DEPDELLEDP YQGQRVHCWV LLRSGKRDVQ QNIFIEPSTG RMYSPSTCPY ECV DAVFNN LNFWINMKPE CEVKNLNFEE MDSSLNWEYV MLDTLLFNKG NGNDEEGNEN IDDPLNKQAQ RDPEQEQLQD IAQL LDMPP PWPPKIYIDK EKFLMGTPLG EGTVYYNKVK VDNYAPYSQP DGLVQKITIF QDYKRLKVKE YRYFYKHRSD KLSIR RRYP FEFKTIEEYE PQKYENKTIP QALQQWRQVI QIDRHLRIIK YYHNRNHDGL IERVEQIGEK TMMFYQNRDD RVIYRS IRF DNKRAPSSQN NDKVFQDNHV GDVQITKMTQ KFEKNPHYPA NEQIQKMVID LIKDKVIVYY HMNDGEITPI VKIYPRD SM HGFAKLNEPG GDNKIDDPLV QLENTKMVNL EKDCLNQLKQ QEKIARDDEK TIREGEIRLE KTLYDKARDR FKESSKKN D DDLAKDAAAS DYLYPYLEKR KLLGVSEINA QVAMEIKNEV MQKLKERLLS RAEIIQRRLE EQRQVLEQKE AQVAKRQEI DPKQEEEIRD INFKIDILEQ RALRFESMAL QKYEEMDNRL NKDARLAALH RK

UniProtKB: Coiled-coil lobo-like protein, putative

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Macromolecule #9: EF-hand calcium-binding domain protein

MacromoleculeName: EF-hand calcium-binding domain protein / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 22.062066 KDa
SequenceString:
MYDPNTSQTE KQKQEEFLKL KIQEAFNLFV KDKKGIVDKR EIPYIMRYLG QFPSEAQVRD AILPEIEEDE PSEFIKYSKF EPYMLKVLK EREYEPDDPE ALLAAFKLLD QEGKGYIEID MMKTFLEKQG IEFREQETKS FIEFATNKDP NATVIYYEDY I SRLQAFTD KHIESVMKGY NNFQVKK

UniProtKB: EF-hand calcium-binding domain protein

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Macromolecule #10: Dynein regulatory complex protein 9

MacromoleculeName: Dynein regulatory complex protein 9 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 44.990227 KDa
SequenceString: MNQIDAHKLT IILRDATERL TFLDTINRQD DLSSELAGYE ISKLLKKQKN LENQYADLVQ LRTSLTGIQN KKNLIETQTK IVDVAQNLK ESTKKLCRLF KENPDLESDA LKVKKDRIQF MAVIEQLIQM VQNNSLTKFQ SMTTLELEDQ DRLRKLIFRE K ELYQEIKK ...String:
MNQIDAHKLT IILRDATERL TFLDTINRQD DLSSELAGYE ISKLLKKQKN LENQYADLVQ LRTSLTGIQN KKNLIETQTK IVDVAQNLK ESTKKLCRLF KENPDLESDA LKVKKDRIQF MAVIEQLIQM VQNNSLTKFQ SMTTLELEDQ DRLRKLIFRE K ELYQEIKK LQFDRNQENK EYDNEQKDTN LKIQNLKERL LYKKARAQLK NQYKEKEARA EEGTQQRMYE FEQKQLKEKI NN LKQKTDT ENMVHEQLRN FLIEKEDQIK QKSDEWAKKV ETCREQLQEE IDRLTVEKEK KQEELRELRE RDQKEQEEKD RRE RQELEE ADRKQQEELD QIRLDNAIKL IQIEYQEWKD AGGGKKKRKK GKKKKK

UniProtKB: Dynein regulatory complex protein 9

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Macromolecule #11: Dynein regulatory complex protein 10

MacromoleculeName: Dynein regulatory complex protein 10 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 51.315023 KDa
SequenceString: MQNLSKMQSR LHPRPEGEGG GGGGRTKEKG KSLQETHKEM LKNIESTNIG KPTIVEAQRI LKIIDNLSTN LTIFIYLDSE LISKFLDVA KHSKLSKDLV SKLSQRCLDL LKSQAEIEAK FKPYASLLDQ SNKEAEDVEE EKMIDAERLR MQLENNFKDL V RHLRNSPE ...String:
MQNLSKMQSR LHPRPEGEGG GGGGRTKEKG KSLQETHKEM LKNIESTNIG KPTIVEAQRI LKIIDNLSTN LTIFIYLDSE LISKFLDVA KHSKLSKDLV SKLSQRCLDL LKSQAEIEAK FKPYASLLDQ SNKEAEDVEE EKMIDAERLR MQLENNFKDL V RHLRNSPE DFEIIKSMKT NVNPDLNDLV HCIHCQKVIM LKKLSTASEE DENHKAQLRD LEEKIEELEK KKNKIQEDLT KL KEHRQKF SKEKKEELEK LKNEIAEVKA EKEKKASQLK NELDNRLKQL ERDHLAKKDK LDKELQKLED DFAKLKQDHA NDE TKLKTN KRQQQNSLAD NIESYDALMK DQHQKKQEIE EELAKIIEEV DTLKQLFKEI DEEKQREREL EEEFRLKKEQ WEIQ ERRKK EAARCILHFL MARKEKSKKK KKKKGKKKK

UniProtKB: Dynein regulatory complex protein 10

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Macromolecule #12: AAA family ATPase CDC48 subfamily protein

MacromoleculeName: AAA family ATPase CDC48 subfamily protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 101.144125 KDa
SequenceString: MSNMMYNMKW QEAINDLMEQ VTLEYLPLEQ NEQQLGMKYK RDSSEWFHFW ATLYIKYIDI YKKLEDCYDQ LVHPQKRVLL KEMLENVIV RLCETKSQVV KYNTQYDATY KSDYPNLDEL VMDLKLTPDC LDIPIPRYFR EEDKKRLDER NQILDALLLE Y TDTKEPQE ...String:
MSNMMYNMKW QEAINDLMEQ VTLEYLPLEQ NEQQLGMKYK RDSSEWFHFW ATLYIKYIDI YKKLEDCYDQ LVHPQKRVLL KEMLENVIV RLCETKSQVV KYNTQYDATY KSDYPNLDEL VMDLKLTPDC LDIPIPRYFR EEDKKRLDER NQILDALLLE Y TDTKEPQE EQYEDQNTLQ ADMDTAIRII QRYERGRQGI ERANLAKILK KEEEKKLERQ KKLAEGTEIG EETEKDDAAL VI KKYWRGY KSRKLVQDIR EEELLFLGMK KVVEDPTLPE SQYKQAQNKR QKMKYIQEEH EQEFNDEIEN LKRLVKGNEG PDI LDKMRA ERREWIMREL EKNEFKEAPQ DPSEFYNQQV MDPEQQAAEA AKAAEEAKKK GAAKKDDKKK KGKPSELDEF LENN KPTGP SPIVLKLQEQ IEKHSGEWSK RDETNNFEQK HETELAKMLI KPVVEEQIKQ QVDEMIAEEL DIVRLRYDIK KKKQK KPPR PRNKGKNKKK FPGDSSNKGR DPKDILAGLV EKRVAKKLIP ASLMDLKGEQ NVLGKIQEIQ ADENLKKTEA LTDAKL KKA QLEEPSTKMP DPSIAQIRQI IAEYIAFPLG SKYAKEKLDK MNYFFFMGPR GSGKTLAVRA LAHECNAIVL DISPSNI DG QYTDKKQIDG MINSAFKVAK EFQPAIIYCE DFEYIFGQAK KKKSQVNPLF AKMKKPLMDF KKGKFFEPED RVVFIGST N RPWDCSQKEI KSFFDKKIYF PFPNYGTRML LFKTFLEQKK VPLPDNFPIN TIAHITEGWS AGSFKMAIDR VFTERRLQK INEEQIKLSE FIGPLSNVPF TSKEEFKEFK KFNQVVTGLQ ANYEAKKAPA DDQKGDKNKK KK

UniProtKB: AAA family ATPase CDC48 subfamily protein

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Macromolecule #13: Cilia- and flagella-associated protein 91

MacromoleculeName: Cilia- and flagella-associated protein 91 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 76.154328 KDa
SequenceString: MATTTNILHD VVRDQSMVNY VSNRDRPLYF KRPLVPQMTD IPLHISRPPV DQQVDYQTMQ MQQNATIVEA PTKDAFVQTD YRESETQTD PYTPKCFVRD GDHPEVMELK DYKYGKGLPA SIEELEQIEL NREKVWFENS LPPISDEASF NLRRKLMEEQ E LREWSKKE ...String:
MATTTNILHD VVRDQSMVNY VSNRDRPLYF KRPLVPQMTD IPLHISRPPV DQQVDYQTMQ MQQNATIVEA PTKDAFVQTD YRESETQTD PYTPKCFVRD GDHPEVMELK DYKYGKGLPA SIEELEQIEL NREKVWFENS LPPISDEASF NLRRKLMEEQ E LREWSKKE NEIKKFQNEK LYLLQQALIE REKEVEDKSQ ERIEEIRQQK TEHKNRQIAK IQRKKIKIDR KMTKSRKMQG KE TLKRDII EDYANFASRV YAGITHEGLS LDKIANKYEV QPLALGNYEM LQALHEGIRP REFETRVNLK KEIKEIEKNY TRL ENYHRG ELKKAQDEIN GVHEQSKAQQ KQEGNNFSYR NFENKIRPAT PTWKYDTDFN ISPSLITEIQ EYRGPNGVQL MQAA DKREE AVLLLQRLLR GRTTQNIMYE GKKKRTALIE ELLTVAQIEN LEEDKAEEVL MQQHEEKVKN AVLESIQGEV IAETM DELS KELLRIKQER KIQQMVEIAE KDRRIREIEE AGKRQAEEIL RDREDVLHNQ LIRVHQGTVD TYLDWLMSNA LEQSSA RQA TIMTNLRKAK FNLPLEDFER KYNNNQTLIK DLVHSFLIPN VQRSKLKKQI QLEEKRFSEA AKRSLQQTIT RASNKHA NV QN

UniProtKB: Cilia- and flagella-associated protein 91

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Macromolecule #14: Flagella associated protein

MacromoleculeName: Flagella associated protein / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 114.163266 KDa
SequenceString: MDQGEFIQDD NDQDYDQQEG DDENNDYMED PQQDNNYNQN GDNLDDQGQD DDGGQNNLQQ PSVPPEDDED DDDDFPEYAN EQNKRLNEI IKKKRKLIKD ISAKIEEKSD RTKVLQEHLK NVEQELLHTQ ALIDAKNKEI ETEDHMKQIA ERQSGRIQSE L KILEKRSI ...String:
MDQGEFIQDD NDQDYDQQEG DDENNDYMED PQQDNNYNQN GDNLDDQGQD DDGGQNNLQQ PSVPPEDDED DDDDFPEYAN EQNKRLNEI IKKKRKLIKD ISAKIEEKSD RTKVLQEHLK NVEQELLHTQ ALIDAKNKEI ETEDHMKQIA ERQSGRIQSE L KILEKRSI EQQEKLNDVQ NQIFRGNEKM DQYKLEMNWN QEELEQWALA ARQKEEDNLT LEKYKRADEV KIKELNLAIE KL TAEVGRK QNELEKEITE TQAAQIELDK TAEEFKRQHE ERHKLFLQWQ EVTEIISKRD LAIREEGENF ARIKIEIKSN KDA LEERKR ILKEAKEENK RIQMANELME RQNIQQISDN KHIEEQLAEK KADVEILKNQ VSAFASDLSS KKNRIAILSQ ELLA KKQRL NAAQKKYQAH QVKLKNEEIM AKQYENDRSY AEEKYRKNEK EKKELEKEIR TQKENLFKHT QELFKHRERE ANLYG EIQG NMAACRNLQS HITKLNQEFQ RQQELLYNAE YQIQLMERRV ARAKGERTLE EKKDLENEIM NAERELGGVT GQNKEL IES LKNLDDEIRT VKKKLAYVEE ENTKYSSLIE ELILENDMTY QDLNKIIKQK EEVLVQHDTM KLEIKKINES LNGATEK VF NLENQIYQLE MSMQEREKEI EVHKDVLMTE HKAAEEERHK IAVELAERKN KVKNLKIKYE SLVQKNKASN GEVESVHE H SQAYYVIKAA QEREELQRKG DELNAKILKN EKELKALDNT LNHLKNRNSN YRDKFLNKGV TTQHREEAEG LEEQCRAAS QNLFKKRDEL QKLQKEQEED TRRYTEIKNK LERLYEQHQS LDQEISKYQK DIDQQGDKLQ RAQNSLQRNY QLAVNKNQNF INPKNPHMI QVKLDNQNNL YKTLLQGLYS LQQDIPELSS VVDEILKEQR IQNNKAPSSI DINSKRSSQS GRSQRSQRSN I SNQ

UniProtKB: Flagella associated protein

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Macromolecule #15: Coiled-coil protein, putative

MacromoleculeName: Coiled-coil protein, putative / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 105.196055 KDa
SequenceString: MSNQQGPEDN NLEDDMAYLP ADHPLLAKLQ IDLTKQLTDE HERVDQKLIE IDANLKKLEK TKEDIGVRLY SVQQQLAENQ MNFEQAHEN YNWVQKLRIE AEQKLKTESE VYDAKKKELE ELRKKYLKAQ DELSKLTRTL YQINEFNQQM KGQIINTKTN T YRAEENVV ...String:
MSNQQGPEDN NLEDDMAYLP ADHPLLAKLQ IDLTKQLTDE HERVDQKLIE IDANLKKLEK TKEDIGVRLY SVQQQLAENQ MNFEQAHEN YNWVQKLRIE AEQKLKTESE VYDAKKKELE ELRKKYLKAQ DELSKLTRTL YQINEFNQQM KGQIINTKTN T YRAEENVV NLEAQKKKQD LLIDTMNEEI KRQTEQKTIL TAQLISQKEE TEQAKQILKE AHLEMQKIIA SKKNLLERWQ KS LMTMQRM DNALQAIKEA LKGQQELNLQ IGTELNGVNA EIRKETEIQE SLEGKNKKFD YEKDYLQKKY NELQEEKSKL EAQ INLLTQ SLRQTETEAG RAEIDKRNIE DQMNLIETNI MKLHTETKKL WEDLVHQKSE HTTIEKTATN LNKQANQISI EIED KSVEL ENLLNEIARV KIDQLNTLSQ IEVLENKRRE VIKEREEKEI TVATYEVQIR QGHDLNEKKQ HEVGRLNREH DKLSS VQSD MSRGPLEAKR NNLIRKTQEL GKENDLMQRE WIKKQTLLVT QNNRLNKIEE DVSQLKTKQT ILEQKKLRLN NNYRIY EKD IREIQNALKN LRNEMNKLND AIYRNKEKQQ KLDNENFNIK SEFVEKLKEL EKESVKLEVE IDRLKEEKAD LLAEIVE SE RQILLWERKI QLEKEMQDAL DPTVGQTEIQ ELKREIHRME LRLDDLRKKQ EAIIAEMERA VYKRETIQLK YMNKDKTF S NSNSMSQKSS SISAASDNSA QITKKIAQLK TTLNQTTRNA EQMEKAIKNK KIELDDLNAQ IEGNNDNLQK LESDCYNKN IELTKHKLER STNILSISCM QNKAKKLEDL VAGKARLSVP EATLMTKYEE LRDKNQEIKE ALQKLCDDAP QYVEVLNYLI DLNVGDDDE DQEQ

UniProtKB: Coiled-coil protein, putative

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Macromolecule #16: DUF4201 domain-containing protein

MacromoleculeName: DUF4201 domain-containing protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 94.540883 KDa
SequenceString: MASEDGEMPS QFEGIDPEQL TESQMQQYMM EMQRQGLLDS NMEGGQYEEG YEEGQEGEGY GEEYGDQDYD GNQNEYDSQQ DSQQQGHDQ VNEMHQDRYD RRVNSEISGN YEADDETLRK IRRDLLDNIN LERRHRDLQP VYIDLTTNNI SQYYSEYLVN N EHNQDFYE ...String:
MASEDGEMPS QFEGIDPEQL TESQMQQYMM EMQRQGLLDS NMEGGQYEEG YEEGQEGEGY GEEYGDQDYD GNQNEYDSQQ DSQQQGHDQ VNEMHQDRYD RRVNSEISGN YEADDETLRK IRRDLLDNIN LERRHRDLQP VYIDLTTNNI SQYYSEYLVN N EHNQDFYE NAKVRYNNLG ECELCHITAK FEPDADITKE YIYDYFMEIG YLFLESEEEK RIILNPANNH IGIGVFFDEI QI VVVLILS EKVLCIQKIS QPEQNKIEIR GKMLDENFGI YAIRIMNVDD QKKDIKGVGP EFIEYTRSTQ EWMASFELEL YNQ DRMAIE YYTRVSPDSI PYKKKQSKNE KLTYKHLQLR LRTPFQIYPD PKYAAEDEKE RIRKEQEILA HEEQERKERE ENDA KRLKQ SRKDYGDGQY DDDEHGQDDF NSQSDISDKD KHHDSMHAEQ EQNQQAAQQQ QDTISNKEIR QELEMAITEA QRQHD EFML QNHKLQEEIK LLKNKNDGFV DRSNETAMNE HKYLNTLAHV HQIRLDLKQT QTRYNQMSQE LQKKLEQKQK KCNEIK YAF LELKREVAKK AANSRTDKPI PEQQINEWEK AELQKSKELQ ELRLQILRLR NAYVKNQKIL KKKEELAEGL HLIDFEQ LK IENQTLNEKI EERNEELHKL KKKNTTTIQI LTHTREKLGF VQGENGELNS QNVRKDQELD DMRKQLTQQK KTKDKLRS V NLTLKQQTGI VNSEELGQDY RDLRTRVSKL EEEKKRLEQK LRSMHEAIKT ANQISTQNMQ SQNNSLKKPY QPY

UniProtKB: Cilia- and flagella-associated protein 184

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Macromolecule #17: Calmodulin 7-2

MacromoleculeName: Calmodulin 7-2 / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 23.862807 KDa
SequenceString: MDNTGKLEKQ LVTLNDGLPK KPAKEIIEEL KHHLYQDFQK FFSEEKKQQY QNNFALFDRD NDKYINLSEL KELLTSVNIT FPDDELEEL YNEFCLTSPE ADGINEDAVF IIVSKKIRDN DKDEQLTQAF KLVEKAVNDE LAKTPNETKE QEGYIRVEQF K ELLMTLGN ...String:
MDNTGKLEKQ LVTLNDGLPK KPAKEIIEEL KHHLYQDFQK FFSEEKKQQY QNNFALFDRD NDKYINLSEL KELLTSVNIT FPDDELEEL YNEFCLTSPE ADGINEDAVF IIVSKKIRDN DKDEQLTQAF KLVEKAVNDE LAKTPNETKE QEGYIRVEQF K ELLMTLGN RWSEEQANEF LKDINPKSDE RINYLDVVKK LMKR

UniProtKB: Calmodulin 7-2

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Macromolecule #18: Coiled-coil domain-containing protein 153

MacromoleculeName: Coiled-coil domain-containing protein 153 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 22.170178 KDa
SequenceString:
MSKKSGKGKG KNDGDELGAE KEQVLRTKVE SLIQRLGHEQ ERADRAKAAE NELRARLFDL DKDFKNEKDR LFSITSDMTR QYKQMQDEL LNQVNDLNKT VIEKDEEIKK KDQQIQDMTK DYEYKLKKKD DEIQDLKRKI EEMSAEFAKM LKDTLDKMQE R IEMVQWDS DTDPQMMKRL KDMTGLSNN

UniProtKB: Coiled-coil domain-containing protein 153

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Macromolecule #19: Flagellar associated protein

MacromoleculeName: Flagellar associated protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 42.931082 KDa
SequenceString: MNQDKHPEIT DEEQELITVE ELSQKLELLY KDMEQIRREN LLFEAYLARN RKEIAKEDEV SEDKKGKGKK KDKNVDKKSL LLTNEEKFE IAQQEQDALK KQIDDGRIKS DQILETLRAI LEETDMAITE IRKDAFDFQR EILVGGENSR TGKIEAEKII K FFEEKERQ ...String:
MNQDKHPEIT DEEQELITVE ELSQKLELLY KDMEQIRREN LLFEAYLARN RKEIAKEDEV SEDKKGKGKK KDKNVDKKSL LLTNEEKFE IAQQEQDALK KQIDDGRIKS DQILETLRAI LEETDMAITE IRKDAFDFQR EILVGGENSR TGKIEAEKII K FFEEKERQ KDALIAKYSS KRTNLERQIL KTNNQIQKKE EMGDDLKFID FYQLQIENKK YVKEIDDKNK KLLALKISTN RI SQTLKDE KQNLKQELDK GKEYASQMSE RKKKISKIDA QIKSVKKVTS KLEKDRKIYD KQKEIFVQDN QDDVPQIMKY VQY KSKEQQ LLYAIQNLER KIEIAELAYK KANRILQSSQ QFQQK

UniProtKB: Cilia- and flagella-associated protein 263

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Macromolecule #20: L-shape (Unknown-protein)

MacromoleculeName: L-shape (Unknown-protein) / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 50.825766 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #21: WD40 domain protein

MacromoleculeName: WD40 domain protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 151.719828 KDa
SequenceString: MAGLAQHIQL KYIFGMNNEI RNSLQFHDED RIIYPAGYNV VLHDLNDKSQ HYYQGTNEYR GISCLALSPL KRYLALGVKG QRDPAIIIF DTFTQKRRKN LGLNLGNLDR YNIKQWVSIA FPSQNQETKY IFSLSGAGGD VVLCWWWHEK GKCLGSIDLG A QNDIYEIS ...String:
MAGLAQHIQL KYIFGMNNEI RNSLQFHDED RIIYPAGYNV VLHDLNDKSQ HYYQGTNEYR GISCLALSPL KRYLALGVKG QRDPAIIIF DTFTQKRRKN LGLNLGNLDR YNIKQWVSIA FPSQNQETKY IFSLSGAGGD VVLCWWWHEK GKCLGSIDLG A QNDIYEIS FNHSDANSIC VTGNNFFGIY AKIEGTYVRE DNQQKQKPSV QNGENSSAAQ LPQPTLNIQV SAGNNNQNPQ PE IKLENGN LISNLPADIV EKNFVAHLWM QCESYLVVFV ESGEIILCDS RGNFLQIMEQ SPRYFIVSRS GMIKIVAAVA YSK GFYVAC SESIVLQYHY TQDNDKNPFT CVNQFKLKQF VELKEIEIKS ICMNKAEDKL LIGLDNNQIY EIKIKPYHPE TVNN DSSED REVTLINHLN HTGPINSMDI CKRKPIIATC STDKTIKIWD YEKKQIKISW AFNEEAFCLS LHPQGFCVAV GFLDK LRLM NLCIHNSQNT TKNKAYKEIS PFKGCKEIKF SNGGQYFAAV NSTSSNHVIQ VFKFFTGENP SQLVFKGHTG RVKCIA WSS DDSFLLSCGL DGMILAWKLD QDFQHQQTIV PRIVDIHNKG VNFSGLTLTV DNKTIVAVGN DRHIHQAVIN EQQPVDK TD KKLLDINLSC LAFPSSNKLL FAGIQDDARS SGAIRCFIYP LTHGKFTDYQ AHDERGVEKM KITNDDRYII TAGKDGCI M VFEIKDKDAR GMKLKDGYAK YSEEELITRS DLDDLKSTRD GLIVQINEFS NQNAMIGLNS RDDRIRQLET QIENNSQKR KQMFEQLLKS KTMEEQKLIQ EINEIKEQFE QEIQVMDTKY QKEVMSLVEE YENQKRLHEI ENNKNNKKKT KLLQEHSQKL QIIDDQYQK LLEEQITQKE RVEKQINHLQ KEQQEVLMQI SEEKETEIKN LNQKNSKDEQ AITDQGLKAK SDISITKKKI M QQQQEAQD LKEQKQEYER QKEKLKIQNQ ELRDKIDGQK KIILERDRTI GEKEKIIYNL KKKAQDLEKF KFVLDHKIKE LK RDIVPRE DEITKMKQET NNMDQYLKEL NAYNNYLGTV VDELYTTQET MKEDIKQQRQ QISVQQVKIS RFKDDVYSLA QHI LDYDKL VDETERLFMK HVNDKEVKRQ SVEGDIMMEY RSQKKNLEKL VNMFKKSLQK DNQIHKDDKI RIMKDNVDLI REIN TLRKS IKDITKGQQS NIPDQKGQTQ HMPLQTPQLS RKSISQPVLP PISKSAMFEG VTQQNEIQKS HTQINPELVE EKQKV LNSL QTDILELQKV YEALNYEKHN LTQA

UniProtKB: WD40 domain protein

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Macromolecule #22: EF hand protein

MacromoleculeName: EF hand protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 124.971133 KDa
SequenceString: MSSDVKDSSH LEKQNSQEYS NSKQNGEIDI MEMLPQKKMY IIEKQFDQSG KGLKIDEFVR VMLTHLDFDK SDVQKEDQIT LALIDLFKE IDVNGDGTME WEEFSDHIIS LGLLRNDRSF KNVIKNYFPS EHIKDNQKHE TPIEKIVYFD KLKYLVVLEK D STRFKVYN ...String:
MSSDVKDSSH LEKQNSQEYS NSKQNGEIDI MEMLPQKKMY IIEKQFDQSG KGLKIDEFVR VMLTHLDFDK SDVQKEDQIT LALIDLFKE IDVNGDGTME WEEFSDHIIS LGLLRNDRSF KNVIKNYFPS EHIKDNQKHE TPIEKIVYFD KLKYLVVLEK D STRFKVYN ANTAELYYEV NAHKCAVLDA EYIPDQNLIA TSSNDLTINF WDSSFFIPKQ IVSVPEIQLC MRYAKWSTQS QP TYLYTGG SDSIIHIYDT KTLKEKGTIG GWNPFFKRDS QQYGHASPIG DILPINIQTT LVTAGLDANI CLWDAATHLP KKE LRGHEK GVYSLDWSDF SPMNQCLLSA GLDHEAFVWN TYVKEKIFLL RGHNHPLIGV KCLPGTPQVI TADINGMVKV WDVR NFLCM QTINVPTDEL NSFVVTSNQK KRIIFGARRL YYYEYDEPKD QMLTDEKMCL RVLYNSVLLC LITLHPDCIK VWDIR TGKL QSVYRELSTA ELTVCVLDTR KRKLFVGDAD GNIFTVNIKN GAKMKKFEKH NNRMITDLTH WTSKKPATIE IGGDDD DED GTGDSRRVVS CSREETVFIH DEDSSDPSRS CRYKMKQHKS SVNSLSLKQD SELFASASDD GNIVITNLNS YRQETLP ST QYPFEKKKVI FLDPYNALVT SDSEGYVHFY CVNNGKARTR YLLTKKYETT SVVNNKVDKF PVTHLAFNSD NNMLLLSD E LGNVTIWDLT IFLKKLDEIK DDNIQQQKTK QAQTPLKQTP NRASNGFFPT ELDELKQINE KQISLSQMDN LEKYFQDKD IAFVHQLKGA HNDGITWIEV LKEYNMFATS SFDCCCHLWS FNDYKKMGSL ILGHDIMNNN WQLRVDEEKR KVDAQYYMEK LKKKLNKDD QSDDEEKNSQ KQDESMMSDK NFDQKNQKKP KKTEKGEKGE KAPEKPLPEK KKVLTAQEKM EQNKKLKNEL K LEFDPALL NLNKNKKQVL SEKEQIEKAS ELLEKVKKFN DVIANKNKDR RNPLEIDYDD FLRAYQMDEQ VDIYLDQNEE EE EDNNILY DLDDPKNQSI PAGAYTSKGA FKSKNIGRKN KI

UniProtKB: EF hand protein

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Macromolecule #23: CFAP20

MacromoleculeName: CFAP20 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 22.976535 KDa
SequenceString: MFKNTFQSGF LSILYSIGSK PLQIWDKSIR NGHIKRITDQ DILSSVLEIM GTNVSTNYIT APADPKETLG IKLPFLVMII KNLKKYYTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDEGW NQIQFNLSDF TRRAYATNYI ETLRVQIHAN C RIRRIYFS ...String:
MFKNTFQSGF LSILYSIGSK PLQIWDKSIR NGHIKRITDQ DILSSVLEIM GTNVSTNYIT APADPKETLG IKLPFLVMII KNLKKYYTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDEGW NQIQFNLSDF TRRAYATNYI ETLRVQIHAN C RIRRIYFS DRLYSEEELP PEFKLFLPIQ GQNKTNV

UniProtKB: Cilia- and flagella-associated protein 20

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Macromolecule #24: WD40 domain protein

MacromoleculeName: WD40 domain protein / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 152.700203 KDa
SequenceString: MAQQIVQSLQ DEGTQSLKLD FSMKYVLGYR SDFKNNIFQY TMNDQNRIIY PAGNTIVISN NENKQQFINC IPGTKGITCM TLSPSKRLL AWSEDCDSGI IVVFDLIKLD KLEKAKNQNY QEPSDKDKLD KQAEKDRRDR FEKEKREKID KLQKEVKRII T TLDCKSRH ...String:
MAQQIVQSLQ DEGTQSLKLD FSMKYVLGYR SDFKNNIFQY TMNDQNRIIY PAGNTIVISN NENKQQFINC IPGTKGITCM TLSPSKRLL AWSEDCDSGI IVVFDLIKLD KLEKAKNQNY QEPSDKDKLD KQAEKDRRDR FEKEKREKID KLQKEVKRII T TLDCKSRH YVALDFNKIN EKRIVALSCA PDWQLIYFQL DKQKTVIINQ VPLKVGDNMR YTHCFFHPKE EDFIVAIGTG AI KPYKLGA DGQFKQKDPP FVKKESKDQA HSPNYLSYCI LQDGYMVIGT DMGEILLFQP SCEFKTILAS SPKNEQFAIQ CIQ PYSKGF LVGGKECTIL FYEKDVDLKN PYKLCSKKIQ FRDMKAMITS LLLTPNEEKL IVGVDSGQLL QVPFTSDSMQ LNEE NSKCE PLFMPFHSDK ITGLDVCIRK SLVATCSVDK TVRIWNYSDN QLENSKEFEE EAYAVAFHPS GFHIIVAFTE KIRLM NIFE NDLISFKELS VKNCREIQFS NGGHFFAITN VSMVQVFQFY TGENPSNLVF RGSGKVRTIF WEEDDQGFYT GSTDGL VIY WRVDDNGPQK TQIAQFNNLI ITSITGLYNP DTTTQGLERI LFVSGVSSSQ ENEGEKCVYK LVISCRQDKE GREITDN TL KKIYYVSQPE QKIFTGTNVS QIAISHSKKL FFFATEDRPG AIRITKYPFT NEIMEIQSHF GPITRMRISF KDNYIFTA G EDGALIIYEN KEKEYQVKIE NESVEAAAEE FLIPRDQYND QKREIEKLKR QLNEERMKQE QQIKEKMKDR DDKINQLEN QQKDSDNRDL NKYQLLEREK NEMIESYEEK KRMMKLQHEN NKRTIENEYK KKIALEMSRN EELAREKEKE EKRFQSEIQQ YQEEHLRQM EEKRRHYEEQ LAIEKQLYND LHLKREELAY KFDQKRNKLE MEAEELIDQL KEENESKMQV LFKNLEKAEI K KMDRRNDY DTEAQKLEEQ KSKLKGTMED ITSIQETNKM LQKEKESHAK EIDEREKTIR DKGRRIYELK KKTQELEKFK FV LDYKIKE LKRDIGPKEE EIAKMKEQIA NMNSEILHFK RTNANLKLIV TDLRLRQEGM KKEIEDQSKV IQQNNQYIKA FEQ DMSDCH QHIADYKKLK QKVLNLYNQY VQGDDSKKKR LENNDQQKEI MKERAHLETS VNNLKIKHDK NQSVHKSDTT IIMK HNTFL IFEINHLKRE KKKILEDKAK MLMQATQKKK QDGTSRVDID SLEKEIQKNE DEKRKLKEDI EKLRQYNDQI KNQLR QQIQ NQQDDDEDN

UniProtKB: WD40 domain protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

8537

Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: OTHER / Number images used: 211502
FSC plot (resolution estimation)

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