EMDB-40968: Atomic model of the mammalian Mediator complex with MED26 subunit

Basic information

Entry

Database: EMDB / ID: EMD-40968

• Title: Atomic model of the mammalian Mediator complex with MED26 subunit
• Map data: Atomic model of the mammalian Mediator complex with MED26 subunit

Sample

• Complex: Mouse mediator complex
  • Protein or peptide: x 27 types

• Keywords: mouse mediator / complex / GENE REGULATION / MED26 subunit

Function / homology

Function:

positive regulation of T cell extravasation / Nuclear Receptor transcription pathway / negative regulation of smooth muscle cell differentiation / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / androgen biosynthetic process / positive regulation of G0 to G1 transition / retinal pigment epithelium development / mammary gland branching involved in thelarche / G0 to G1 transition / thyroid hormone receptor signaling pathway / Regulation of lipid metabolism by PPARalpha / core mediator complex / regulation of vitamin D receptor signaling pathway / Cytoprotection by HMOX1 / Estrogen-dependent gene expression / ventricular trabecula myocardium morphogenesis / thyroid hormone generation / nuclear retinoic acid receptor binding / mediator complex / positive regulation of keratinocyte differentiation / embryonic heart tube development / camera-type eye development / cellular response to thyroid hormone stimulus / positive regulation of hepatocyte proliferation / embryonic hindlimb morphogenesis / nuclear vitamin D receptor binding / peroxisome proliferator activated receptor binding / limb development / lens development in camera-type eye / positive regulation of intracellular estrogen receptor signaling pathway / embryonic hemopoiesis / nuclear thyroid hormone receptor binding / mammary gland epithelial cell proliferation / megakaryocyte development / histone acetyltransferase binding / cellular response to hepatocyte growth factor stimulus / cortical actin cytoskeleton / termination of RNA polymerase II transcription / LBD domain binding / fat cell differentiation / epithelial cell proliferation involved in mammary gland duct elongation / skeletal muscle cell differentiation / mammary gland branching involved in pregnancy / somatic stem cell population maintenance / monocyte differentiation / blastocyst development / general transcription initiation factor binding / negative regulation of neuron differentiation / hematopoietic stem cell differentiation / embryonic placenta development / negative regulation of keratinocyte proliferation / positive regulation of transcription initiation by RNA polymerase II / animal organ regeneration / erythrocyte development / nuclear retinoid X receptor binding / nuclear receptor-mediated steroid hormone signaling pathway / negative regulation of fibroblast proliferation / ubiquitin ligase complex / histone acetyltransferase activity / RNA polymerase II preinitiation complex assembly / keratinocyte differentiation / cellular response to epidermal growth factor stimulus / peroxisome proliferator activated receptor signaling pathway / lactation / positive regulation of erythrocyte differentiation / nuclear receptor coactivator activity / liver development / promoter-specific chromatin binding / nuclear estrogen receptor binding / nuclear receptor binding / protein-DNA complex / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / animal organ morphogenesis / mRNA transcription by RNA polymerase II / cell morphogenesis / brain development / chromatin DNA binding / transcription coactivator binding / protein import into nucleus / transcription corepressor activity / ubiquitin protein ligase activity / actin binding / heart development / angiogenesis / in utero embryonic development / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / transcription by RNA polymerase II / transcription coactivator activity / cytoskeleton / nuclear body / protein ubiquitination / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin binding / positive regulation of cell population proliferation / protein-containing complex binding / positive regulation of gene expression

Domain/homology:

Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med19, metazoa / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / : / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator complex subunit 16, central helical bridge / Mediator complex, subunit Med23 / Mediator complex subunit 23 / Mediator of RNA polymerase II transcription subunit 15 / : / : / ARC105/Med15 mediator subunit N-terminal KIX domain / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator complex subunit 30 / Mediator complex subunit 28 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / : / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / Mediator complex, subunit Med7 superfmaily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / TFIIS N-terminal domain profile. / TFIIS/LEDGF domain superfamily / Coactivator CBP, KIX domain superfamily / von Willebrand factor A-like domain superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

Component:

Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 20

• Biological species: Mus musculus (house mouse)
• Method: single particle reconstruction / cryo EM / Resolution: 4.68 Å
• Authors: Zhao H / Asturias F

Funding support

United States, 2 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01-67167	United States
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS)		United States

• Citation: Journal: To Be Published; Title: An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II; Authors: Zhao H / Li J / Xiang Y / Malik S / Young N / Ryner M / Conte A / Roeder R / Shi Y / Casellas R / Asturias F

History

Deposition	Jun 2, 2023	-
Header (metadata) release	Jun 12, 2024	-
Map release	Jun 12, 2024	-
Update	Jun 12, 2024	-
Current status	Jun 12, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_40968.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Atomic model of the mammalian Mediator complex with MED26 subunit
• Voxel size: X=Y=Z: 1.11 Å

Density

Contour Level	By AUTHOR: 0.054
Minimum - Maximum	-0.070911705 - 0.30787417
Average (Standard dev.)	-0.00021929563 (±0.008145886)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 560 560 560 Spacing 560 560 560
Cell	A=B=C: 621.60004 Å α=β=γ: 90.0 °

Supplemental data

Half map: Half Map 1

• File: emd_40968_half_map_1.map
• Annotation: Half Map 1

Half map: Half Map 2

• File: emd_40968_half_map_2.map
• Annotation: Half Map 2

Sample components

Entire : Mouse mediator complex

• Entire: Name: Mouse mediator complex

Components

• Complex: Mouse mediator complex
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 16
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 23
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 24
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 25
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 26
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 27
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 28
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 29
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 30
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
  • Protein or peptide: Unknown Peptide

Supramolecule #1: Mouse mediator complex

• Supramolecule: Name: Mouse mediator complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 1.0 MDa

Macromolecule #1: Mediator of RNA polymerase II transcription subunit 1

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 167.354859 KDa

Sequence

String:

MKAQGETEDS ERLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNG LGSHLSASGT ECYITSDMFY VEVQLDPAGQ LCDVKVAHHG ENPVSCPELV QQLREKNFEE FSKHLKGLVN L YNLPGDNK LKTKMYLALQ SLEQDLSKMA IMYWKATNAA PLDKILHGSV GYLTPRSGGH LMNMKYYASP SDLLDDKTAS PI ILHEKNV PRSLGMNASV TIEGTSAMYK LPIAPLIMGS HPADNKWTPS FSAVTSANSV DLPACFFLKF PQPIPVSKAF VQK LQNCTG IPLFETPPTY LPLYELITQF ELSKDPDPLP LNHNMRFYAA LPGQQHCYFL NKDAPLPDGQ SLQGTLVSKI TFQH PGRVP LILNMIRHQV AYNTLIGSCV KRTILKEDSP GLLQFEVCPL SESRFSVSFQ HPVNDSLVCV VMDVQDSTHV SCKLY KGLS DALICTDDFI AKVVQRCMSI PVTMRAIRRK AETIQADTPA LSLIAETVED MVKKNLPPAS SPGYGMTTGN NPMSGT TTP TNTFPGGPIT TLFNMSMSIK DRHESVGHGE DFSKVSQNPI LTSLLQITGN GGSTIGSSPT PPHHTPPPVS SMAGNTK NH PMLMNLLKDN PAQDFSTLYG SSPLERQNSS SGSPRMEMCS GSNKAKKKKS SRVPPDKPKH QTEDDFQREL FSMDVDSQ N PMFDVSMTAD ALDTPHITPA PSQCSTPPAT YPQPVSHPQP SIQRMVRLSS SDSIGPDVTD ILSDIAEEAS KLPSTSDDC PPIGTPVRDS SSSGHSQSAL FDSDVFQTNN NENPYTDPAD LIADAAGSPN SDSPTNHFFP DGVDFNPDLL NSQSQSGFGE EYFDESSQS GDNDDFKGFA SQALNTLGMP MLGGDNGEPK FKGSSQADTV DFSIISVAGK ALGAADLMEH HSGSQSPLLT T GELGKEKT QKRVKEGNGT GASSGSGPGS DSKPGKRSRT PSNDGKSKDK PPKRKKADTE GKSPSHSSSN RPFTPPTSTG GS KSPGSSG RSQTPPGVAT PPIPKITIQI PKGTVMVGKP SSHSQYTSSG SVSSSGSKSH HSHSSSSSSL ASASTSGKVK SSK SEGSSS SKLSGSMYAS QGSSGSSQSK NSSQTGGKPG SSPITKHGLS SGSSSTKMKP QGKPSSLMNP SISKPNISPS HSRP PGGSD KLASPMKPVP GTPPSSKAKS PISSGSSGSH VSGTSSSSGM KSSSGSASSG SVSQKTPPAS NSCTPSSSSF SSSGS SMSS SQNQHGSSKG KSPSRNKKPS LTAVIDKLKH GVVTSGPGGE DPIDSQMGAS TNSSNHPMSS KHNTSGGEFQ SKREKS DKD KSKVSASGGS VDSSKKTSES KNVGSTGVAK IIISKHDGGS PSIKAKVTLQ KPGESGGDGL RPQIASSKNY GSPLISG ST PKHERGSPSH SKSPAYTPQN VDSESESGSS IAERSYQNSP SSEDGIRPLP EYSTEKHKKH KKEKKKVRDK DRDKKKSH S MKPENWSKSP ISSDPTASVT NNPILSADRP SRLSPDFMIG EEDDDLMDVA LIGN

UniProtKB: Mediator of RNA polymerase II transcription subunit 1

Macromolecule #2: Mediator of RNA polymerase II transcription subunit 4

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.817475 KDa

Sequence

String:

MAASSSGEKE KERMGGVSGM AGLGSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQLE EENQVLELLI HRDGDFQELM KLALNQGKV HHEMQALEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV PGDPRRPYPT DLEMRSGLLG QMNNPSTSGV NGHLPGDALA AGRLPDVLAP QYPWQSNDMS VNMLPPNHSS DF LLEPPGH NKENEDDVEV MSTDSSSSSS DSD

UniProtKB: Mediator of RNA polymerase II transcription subunit 4

Macromolecule #3: Mediator of RNA polymerase II transcription subunit 6

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 28.473553 KDa

Sequence

String:

MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQEKVK P KAKRKEEP SSIFQRQRVD ALLIDLRQKF PPRFVQQKSG EKPVPVDQAK KEAEPLPETV KSEEKESTKN IQQTVSTKGP PE KRMRLQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

Macromolecule #4: Mediator of RNA polymerase II transcription subunit 7

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 27.244252 KDa

Sequence

String:

MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ASLPDDLPHS EAGMRVKAEP MDTDDNSNCP GQNEQQRESS GHRRDQIIEK DAALCVLIDE MNERP

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

Macromolecule #5: Mediator of RNA polymerase II transcription subunit 8

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.232008 KDa

Sequence

String:

MQREEKQLEA SLDALLNQVA DLKNSLGSFI YKLENEYDRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP NKQTFNPGDT NALVAAVAFG KGLSNWRPSG SSGPGQPGQP GAGTILAGAS GLPQVQMPGA PNQQQPMLSG VQ MAQAGQP GKMPSGIKTN IKSASMHPYQ R

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

Macromolecule #6: Mediator of RNA polymerase II transcription subunit 9

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.729063 KDa

Sequence

String:

MASSGVAGGR QAEDTLQPPP ELLPESKPPP PPQPLPVAAL PPPAAPRPQS PAGAKEENYS FLPLVHNVIK CMDKDSPDLH QDLNALKTK FQELRKLIGT MPGIHVSPEQ QQQQLHSLRE QVRTKNELLQ KYKSLCMFEI PKD

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

Macromolecule #7: Mediator of RNA polymerase II transcription subunit 10

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 10; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.683943 KDa

Sequence

String:

MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLSQK LNFIVTGLQD IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERAL AKNEQVKGKI DTMKKFKSLL IQELSKVFPE DMAKYRSIRG EDHPPS

UniProtKB: Mediator of RNA polymerase II transcription subunit 10

Macromolecule #8: Mediator of RNA polymerase II transcription subunit 11

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 11; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.147803 KDa

Sequence

String:

MATYSLANER LRALEDIERE IGAILQNAGT AILELSKEKT NERLLDRQAA AFTTSVQHVE AELSAQIRYL TQVATGQPHE GSSYSSRKD CQMALKRVDY ARLKISDVAR TCEQMLEN

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

Macromolecule #9: Mediator of RNA polymerase II transcription subunit 14

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 14; type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 161.144766 KDa

Sequence

String:

MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKI EIVQFASRTR QLFVRLLALV KWANDAGKVE KCAMISSFLD QQAILFVDTA DRLASLARDA LVHARLPSFA I PYAIDVLT TGSYPRLPTC IRDKIIPPDP ITKIEKQATL HQLNQILRHR LVTTDLPPQL ANLTVANGRV KFRVEGEFEA TL TVMGDDP EVPWRLLKLE ILVEDKETGD GRALVHSMQI DFIHQLVQSR LFADEKPLQD MYNCLHCFCL SLQLEVLHSQ TLM LIRERW GDLVQVERYH AGKSLSLSVW NQQVLGRKTG TASVHKVTIK IDENDVSKPL QIFHDPPLPA SDSKLVERAM KIDH LSIEK LLIDSVHARA HQRLQELKAI LRSFNANESS SIETALPALI VPILEPCGNS ECLHIFVDLH SGMFQLMLYG LDPAT LEDM EKSLNDDMKR IIPWIQQLKF WLGQQRCKQS IKHLPTITTE TLQLANYSTH PIGSLSKNKL FIKLTRLPQY YIVVEM LEV PNKPTQLSYN YYFMSVSTAD REDSPVMALL LQQFKDNIQD LMSYTKTGKQ TRTGTKHKLS DDPCPIDSKK AKRSGEM CA FNKVLAHFVA MCDTNMPFVG LRLELSNLEI PHQGVQVEGD GFNHAIRLLK IPPCKGISEE TQKALDRSLL DCTFRLQG R NNRTWVAELV FANCPLNGTS TREQGPSRHV YLTYENLLSE PVGGRKVVEM FLNDWSSIAR LYECVLEFAR SLPEIPAHL NIFSEVRVYN YRKLILCYGT TKGSSISIQW NSIHQKFHIA LGTVGPNSGC SNCHNTILHQ LQEMFNKTPN VVQLLQVLFD TQAPLNAIN KLPTVPMLGL TQRTNTAYQC FSILPQSSTH IRLAFRNMYC IDIYCRSRGV VAIRDGAYSL FDNSKLVEGF Y PAPGLKTF LNMFVDSNQD ARRRSVNEDD NPPSPIGGDM MDSLISQLQP PQQQPFPKQP GTSGAYPLTS PPTSYHSTVN QS PSMMHTQ SPGNLHAASS PSGALRAPSP ASFVPTPPPS SHGISIGPGA SFASPHGTLD PSSPYTMVSP SGRAGNWPGS PQV SGPSPA TRLPGMSPAN PSLHSPVPDV SHSPRAGTSS QTMPTNMPPP RKLPQRSWAA SIPTILTHSA LNILLLPSPT PGLV PGLAG SYLCSPLERF LGSVIMRRHL QRIIQQETLQ LINSNEPGVI MFKTDALKCR VALSPKTNQT LQLKVTPENA GQWKP DELQ VLEKFFETRV AGPPFKANTL IAFTKLLGAP THILRDCVHI MKLELFPDQA TQLKWNVQFC LTIPPSAPPI APPGTP AVV LKSKMLFFLQ LTQKTSVPPQ EPVSIIVPII YDMASGTTQQ ADIPRQQNSS VAAPMMVSNI LKRFAEMNPP RQGECTI FA AVRDLMANLT LPPGGRP

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

Macromolecule #10: Mediator of RNA polymerase II transcription subunit 15

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 15; type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 86.68293 KDa

Sequence

String:

MDVSGQETDW RSAAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLT GGPTPGAAGI GMPPRGPGQS LGGMGGLGAM GQPLPLSGQP PPGTSGMAPH GMAVVSTATP QTQLQLQQVA L QQQQQQQQ QQQFQQQQAA LQQQQQQQQQ QQQQQQFQAQ QNAMQQQFQA VVQQQQLQQQ QQQQHLIKLH HQSQQQQIQQ QQ LQRMAQL QLQQQQQQQQ QQALQAQPPM QQPSMQQPQP PPSQALPQQL SQLHHPQHHQ PPPQAQQSPI AQNQPPQIPP QSQ SQPLVS QAQALPGPML YAAQQQLKFV RAPMVVQQPQ VQPQVQQVQP QVQPQAAVQA AQSAQMVAPG VQMIAEALAQ GGMH VRARF PPTSTMSAGP SSSISLGGQP TTQVSQSSLT MLSSPSPGQQ VQTPQSMPPP PQPSPQPGSQ PNSNVSSGPA PSPSS FLPS PSPQPSQSPV TARTPQNFSV PSPGPLNTPV NPSSVMSPAG SSQAEEQQYL DKLKQLSKYI EPLRRMINKI DKNEDR KKD LSKMKSLLDI LTDPSKRCPL KTLQKCEIAL EKLKNDMAVP TPPPPPVLPT KQQDLCQPLL DAVLANIRSP VFNHSLY RT FVPAMMAIHG PPIVSPVVCS RKRRFEEDER QSIPNVLQGE VARLDPKFLV NLDPSHCSNN GTVHLICKLD DKDLPSVP P LELSVPADYP AQSPMWIDRQ WQYDANPFLQ SVHRCMTSRL LQLPDKHSVT ALLNTWAQSI HQACLSAA

UniProtKB: Mediator of RNA polymerase II transcription subunit 15

Macromolecule #11: Mediator of RNA polymerase II transcription subunit 16

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 16; type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 91.890484 KDa

Sequence

String:

MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADA DGQIKCWSMA DHLANSWESS VGSQVEGDPI VALSWLHNGV KLALHVEKSG ASSFGEKFSR VKFSPSLTLF G GKPMEGWI AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAA ADGSSASPVK FYKVCVSVVS EK CRIDTEI LPSLFMRCTT DPNRKDRFPA ITHLKFLARD MSEQVLLCAS SQTSSLVECW SLRKEGLPVN NIFQQISPVV GDK QPMILK WRILSATNDL DRVSAVALPK LPISLTNTDL KVASDTQFYP GLGLALAFQD GSVHMVHRLS LQTMAVFYSS APRS LDEPA LKRPRTTCPA VHFKAMQLSW TSLALVGIDN HGKLSMLRIS PSLGHPLEPK LALQHLLFLL EYCMVTGYDW WDILL HVQP GMVQSLVERL HEEYTRQKPA LQQVLSTRIL AMKASLCKLS PCTVARVCDY HTKLFLMAIT STLKSLLRPH FLNTPD KSP GDRLAEICAK ITDVDIDKVM INLKTEEFVL DMNTLQALQQ LLQWVGDFVL YLLVSLPNQG SPLRPGHSFL RDGTSLG ML RELMVVIRIW GLLKPSCLPV YTATSDTQDS MSLLFRLLTK LWICCRDEGA ASEPDEGLVD ECCLLPSQLL VPNLDWLP A SDGLVSRLQP KQPLRLRFGR APTLPSSTST LQLDGLTRAP GQPKIDHLRR LHLGAYPTEE CKACTRCGCV TMLKSPNKT TAVTQWEQRW IKNCLCGGLW RRVPLSCS

UniProtKB: Mediator of RNA polymerase II transcription subunit 16

Macromolecule #12: Mediator of RNA polymerase II transcription subunit 17

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 17; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 72.555609 KDa

Sequence

String:

MSGVRAVRIS IESACEKQVQ EVGLDGTETY LQPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGPDGDAPDW GGAGADQDDE EGLVKFQPS LWPWDSVRNN LRSALTEMCV LYDVLSIVRD KKFMTLDPVS QDALPPKQSP QTLQLISKKK SLAGAAQILL K GAERLTKS VAENQENKLQ RDFNSELLRL RQHWKLRKVG DKILGDLSYR SAGSLFPHHG TFEVIKNTDI DLDKKIPEDY CP LDVQIPS DLEGSAYIKV SIQKQAPDIG DLGTVNLFKR PLPKSKPGSP HWQTKLEAAQ NVLLCKEIFA QLSREAVQIK SQI PHIVVK NQIISQPFPS LQLSISLCHS SDDKKSQKCA AEKPGQEDHL YVLEHNLHLL IREFHKQTLS SIVMPHPASA PFGH KRMRL SGPQAFDKNE INSIQSTEGL LEKIIKQAKH IFLRSRTAAT IDSLASRIED PQIQAHWSNI NDVYESSVKV LITSQ GYEQ ICKSIQLQLN IGVEQVRVVH RDGRVIMLSH QEQELQDFLL SQMSQHQVHA VQQLAKVMGW QVLSFSNHVG LGPIES IGN ASAITVASPS GDYAISVRNG PESGSKIMVQ FPRNQCKDLP KSDVLQDSKW SHLRGPFKEV QWNKMEGRNF VYKMELL MS ALSPCLL

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

Macromolecule #13: Mediator of RNA polymerase II transcription subunit 18

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 18; type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.675652 KDa

Sequence

String:

MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKVVVYKIF RILVPGNTDS T EALSLSYL VELSVVAPAG QDMVSDDMRN FAEQLKPLVH LEKIDPKRLM

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

Macromolecule #14: Mediator of RNA polymerase II transcription subunit 19

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 19; type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 25.866531 KDa

Sequence

String:

MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS APAGADKSTA GSGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILGG SFNPITGTML SGFRLHTGPL P EQCRLMHI QPPKKKNKHK HKQSRTQDPV PPETPSDSDH KKKKKKKEED PERKRKKKEK KKKKNRHSPD HPGMGSSQAS SS

UniProtKB: Mediator of RNA polymerase II transcription subunit 19

Macromolecule #15: Mediator of RNA polymerase II transcription subunit 20

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 20; type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.213775 KDa

Sequence

String:

MGVTCVSQMP VAEGKSLQQT VELLTKKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQAG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH APGAPTVFGN RHDAVYGPAD TMIQYMELFN KIRKQQQVPV AGIR

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

Macromolecule #16: Mediator of RNA polymerase II transcription subunit 21

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 21; type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.600307 KDa

Sequence

String:

MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFSNIQT AINKDQPANP TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAAS LYKLEEENHE AATCLEDVVY RGDMLLEKIQ SALADIAQSQ LKTRSVTHSH SLPDS

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

Macromolecule #17: Mediator of RNA polymerase II transcription subunit 22

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 22; type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.30475 KDa

Sequence

String:

MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRALQ EECDRKLITL RDEVSIDLYE LEEEYYSSSS SLCEANDLPL CEAYWRLDLD A DSADGLSA PLLASPETGA GPLQSAAPVH SHGGGPGPTE HT

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

Macromolecule #18: Mediator of RNA polymerase II transcription subunit 23

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 23; type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 156.265656 KDa

Sequence

String:

METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRMVCE SLINSDSLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK AILEKILTIP NTVSSAVVQQ L LAAREVIA YILERNACLL PAYFAVTEIR KLYPEGKLPH WLLGNLVSDF VDTFRPTARI NSICGRCSLL PVVNNSGAIC NS WKLDPAT LRFPLKGLLP YDKDLFEPQT ALLRYVLEQP YSRDMVCNML GLNKQHKQRC PVLEDQLVDL VVYAMERSET EEK FDDGGT SQLLWQHLSS QLIFFVLFQF ASFPHMVLSL HQKLAGRGLI KGRDHLMWVL LQFISGSIQK NALADFLPVM KLFD LLYPE KECIPVPDIN KPQSTHAFAM TCIWIHLNRK AQNGDSTLQI PIPHSLKLHH EFLQQSLRNK SLQMNDYKIA LLCNA YSTN SECFTLPMGA LVETIYGNGI MRVPLPGTSC LASASVTPLP MNLLDSLTVH AKMSLIHSIA TRVIKLAHTK SSVALA PAL VETYSRLLVY MEIESLGIKG FISQLLPTVF KSHAWGILHT LLEMFSHRMH HIQPHYRVQL LSHLHTLAAV AQTNQNQ LH LCVESTALRL ITALGSSEVQ PQFTRFLNDP KTVLSAESEE LNRALILTLA RATHVTDFFT GSDSIQGTWC KDILQTIM N FTPHNWASHT LSCFPAPLQA FFKQNNVPQE SRFNLKKNVE EEYRKWKSMT DENEIITQFS VQGFPPLFLC LLWKMLLET DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLL LLKPNDFRNR VSDFVKENSP EHWLQSDWHT KHMSYHKKYP EKLYFEGLAE QVDPPVPIQS PYLPIYFGNV C LRFLPVFD IVIHRFLELL PVSKSLETLL DHLGGLYKFH DRPVTYLYNT LHYYEMCLRN RDHLKRKLVH AIIGSLKDNR PQ GWCLSDT YLKHAMNARE DNPWVPEDSY YCKLIGRLVD TMAGKSPGPF PNCDWRFNEF PNPAAHALHV TCVELMALAV PGK DVGNAL LNVVLKSQPL VPRENITAWM NAIGLIITAL PEPYWIVLHD RIVNVISSSS LTSETEWVGY PFRLFDFTAC HQSY SEMSC SYTLALAHAV WHHSSIGQLS LIPKFLTEAL LPVVKTEFQL LYVYHLVGPF LQRFQQERTR CMIEIGVAFY DMLLN VDQC STHLNYMDPI CDFLYHMKYM FTGDSVKEQV EKIICNLKPA LKLRLRFITH ISKMEPAVPP QALNSGSPAP QSNQVP ASL PVTQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 23

Macromolecule #19: Mediator of RNA polymerase II transcription subunit 24

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 24; type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 110.106867 KDa

Sequence

String:

MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLAEALLEQA MIGPSPNPLI LSYLKYAISS QMVSCSSVLT AISKFDDFS RDLCVQALLD IMDMFCDRLS CHGKAEECIG LCRALLSALH WLLRCTAASA ERLQEGLEAG TPAPGEKQLA L CLQCLEKT LSSTKNRALL HIAKLEEASS WTAIEHSLLK LGEILANLSN PQLRSQAERC GTLIRSIPSM LSVHSEQLHK TG FPTIHAL ILLEGTMNLT GEMQPLVEQL MMVKRMQHIP TPLFVLEIWK ACFVGLIESP EGTQELKWTA FTYLKIPQVL VKL KKYFHG EKDFTEDVNC AFEFLLKLTP LLDKADQRCN CDCTNFLLQE CNKQGLLSEV NFASLVGKRT ADRDPQLKSS ENAN IQPNP GLILRAEPTV TNILKTMDAD HSKSPEGLLG VLGHMLSGKS LDLLLAAAAA TGKLKSFARK FINLNEFTTH GSGES TKTA SVRALLFDIS FLMLCHVAQT YGSEVILSES SSGEEVPFFE TWMQTCMPEE GKILNPDHPC FRPDSTKVES LVALLN NSS EMKLVQMKWH EACLSISAAI LEILNAWENG VLAFESIQKI TDNIKGKVCS LAVCAVAWLV AHVRMLGLDE REKSLQM IR QLAGPLYSEN TLQFYNERVV IMNSILEHMC ADVLQQTATQ IKFPSTGVDT MPYWNLLPPK RPIKEVLTDI FAKVLEKG W VDSRSIHILD TLLHMGGVYW FCNNLIKELL KETRKEHTLR AVQLLYSIFC LDMQQVTLVL LGHILPGLLT DSSKWHSLM DPPGTALAKL AVWCALSSYS SHKGQASSRQ KKRHREDIED YVSLFPVEDM QPSKLMRLLS SSDDDANILS SPTDRSMNSS LSASQLHTV NMRDPLNRVL ANLFLLISSI LGSRTAGPHT QFVQWFMEEC VGCLEQDSRG SILQFMPFTT VSELVKVSAM S SPKVVLAI TDLSLPLGRQ VAAKAIAAL

UniProtKB: Mediator of RNA polymerase II transcription subunit 24

Macromolecule #20: Mediator of RNA polymerase II transcription subunit 25

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 25; type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 78.173328 KDa

Sequence

String:

MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTS SAYEFVTWLD GIKFMGGGGE SCSLIAEGLS TALQLFDDFK KMREQIGQTH RVCLLICNSP PYLLPAVEST T YSGCTTES LVQKIGERGI HFSIVSPRKL PALRLLFEKA APPALLEPLQ QPADVSQDPR HMVLVRGLVL PVGGSSTSGS LQ TKQAVPL PPAPASAATL SAAPPQALPP VPPQYQVPGN LSAAQVAAQN AVEAAKSQKA GLGPRFSPIN PLQQAAPGVG PPF SQAPAP PLAPVPPGAP KPPPASQPSL VSTVAPGPVL AAPAQPGAPS LAGTVTPGGV NGPSAAQLGG PALGGQQSVS NKLL AWSGV LEWQEKPKPA SVDANTKLTR SLPCQVYVNH GENLKTEQWP QKLIMQLIPQ QLLTTLGPLF RNSRMVQFHF TNKDL ESLK GLYRIMGNGF AGCVHFPHTA PCEVRVLMLL YSSKKKIFMG LIPYDQSGFV NGIRQVITNH KQVQQQKLEQ QRGMGA QQA PPVLGPILEE QARPPQNLLQ LRAPQPQPQG AVGASAATGQ PQPQGATQAP TGAPQGPPGA APGPPPSGPI LRPQNPG AN PQLRSLLLNP APPQTGVPPP QASLHHLQPP GAPTLLPPHQ SMGQPQLGPQ LLHPPPAQSW PTQLPQRAPL PGQMLLSG G PRGPVPQPGL QPSVMEDDIL MDLI

UniProtKB: Mediator of RNA polymerase II transcription subunit 25

Macromolecule #21: Mediator of RNA polymerase II transcription subunit 26

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 26; type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 64.773477 KDa

Sequence

String:

MTAAPASPQQ MRDRLLQAID SQSNIRNMVA VLEVISSLER YPITKEALEE TRLGKLINDV RKKTKNEELA KRAKRLLRSW QKLIEPVHQ NEVALRALAG AAGSANGGAH NCRPEMGVAG APKSIHDLKN RNDIQRLPGQ RLDRLGSRKR RGDQRDLGHP G PPHKVSKG SPDPLVPNAS PLPTNGISGS PESLPSPLDG SGHLGPDGSR LEPSDNEKHS TKIPVNAVRP RPSSPGLGKP PV PCLQTKA AQLQQLDRAD ESPGPPYPRG SSRCSFSPRN SRHEGSFSRH RSSYIPKGQV SSPSPWPQPP DNTQVPSPLP LAQ PPTPPV RRQELLPNAE SPVHWPEQSE GHPRLTGPAC RAGFSPDSSK ADSDATSSGG SDSKKKKRYR PRDYTVNLDG QVAE AGVKP VRLKERKLTF DPMTRQIRPL TQKEPVRADS PVPTEQLPRT ELEQQEVKAS LQSPFEQTNW KELSRNEIIQ SYLSR QSSL LSSSGAQTPG AHHFMAEYLK QEESSRQGAR QPHVLLPLPT PTDLPGLTRE VTQDDLDRIQ AQQWPGVNGC EDTQGN WYD WTQCISLDPH GDDGRLNILP YVCLD

UniProtKB: Mediator of RNA polymerase II transcription subunit 26

Macromolecule #22: Mediator of RNA polymerase II transcription subunit 27

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 27; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 35.343605 KDa

Sequence

String:

MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIANFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR IDRMFPEMSI HLSRPNGTSA MLLVTLGKVL KVIVVMRSLF IDRTIVKGYN ESVYTEDGKL DIWSKSSYQV FQ KVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 27

Macromolecule #23: Mediator of RNA polymerase II transcription subunit 28

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 28; type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 19.558145 KDa

Sequence

String:

MAASLGGMFT GQPPGPPPPP PGLPGQASLL QAAPGAPRPS NSTLVDELES SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIAR QTECFFLQKR LQLSVQKPDQ VIKEDVSELR SELQRKDALV QKHLTKLRHW QQVLEDINVQ HKKPADMPQG S LAFLEQAS ANIPAPLKQT

UniProtKB: Mediator of RNA polymerase II transcription subunit 28

Macromolecule #24: Mediator of RNA polymerase II transcription subunit 29

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 29; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.027727 KDa

Sequence

String:

MAAPQPQAAA VSSASGVSGP GSAGGPGPQQ QPQPTQLVGS AQSGLLQQQQ QDFDPVQRYK MLIPQLKESL QTLMKVAAQN LIQNTNIDN GQKSSDAPLQ RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV QPDSLPYPQY L AVIKAQIT CAKDIHTALL DCANKVTGKT TAPSTGPGGS L

UniProtKB: Mediator of RNA polymerase II transcription subunit 29

Macromolecule #25: Mediator of RNA polymerase II transcription subunit 30

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 30; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.383354 KDa

Sequence

String:

MSTPPLAPTG MASGPFGGPQ AQQAAREVNT ATLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD HLRQLSILF RKLRLVYDKC NENCGGMDPI PVEQLIPYVD EDGSKNDDRA GPPRFASEER REIVEVNKKL KQKNQQLKQI M DQLRNLIW DINAMLAMRN

UniProtKB: Mediator of RNA polymerase II transcription subunit 30

Macromolecule #26: Mediator of RNA polymerase II transcription subunit 31

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 31; type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.792077 KDa

Sequence

String:

MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKE PEYAKYLKYP QCLHMLELLQ YEHFRKELV NAQCAKFIDE QQILHWQHYS RKRVRLQQAL AEQQQQNNTA GK

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

Macromolecule #27: Unknown Peptide

• Macromolecule: Name: Unknown Peptide / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 1.720111 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.1 mg/mL
• Buffer: pH: 7.9
• Sugar embedding: Material: ice
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: HOMEMADE PLUNGER

Electron microscopy

• Microscope: FEI TALOS ARCTICA
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 22.5 e/Ų
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.7000000000000001 µm
• Experimental equipment: Model: Talos Arctica / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.68 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 199078
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD