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- EMDB-40194: The Type 9 Secretion System Extended Translocon - SprA-PorV-PPI-R... -
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Open data
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Basic information
Entry | ![]() | |||||||||||||||||||||
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Title | The Type 9 Secretion System Extended Translocon - SprA-PorV-PPI-RemZ-SkpA-SprE complex | |||||||||||||||||||||
![]() | Composite volume used for refinement of coordinates created from EMD-29911 and EMD-40085 the associated consensus volume is EMD-40086 | |||||||||||||||||||||
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![]() | T9SS / Type IX Secretion System Translocon / Protein Secretion PorV - SprA - PPI - RemZ Complex / MEMBRANE PROTEIN | |||||||||||||||||||||
Function / homology | ![]() peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / unfolded protein binding / cytoplasm Similarity search - Function | |||||||||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.2 Å | |||||||||||||||||||||
![]() | Deme JC / Lea SM | |||||||||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: The Type 9 Secretion System caught in the act of transport Authors: Lauber F / Deme JC / Liu X / Kjaer A / Miller HL / Alcock F / Lea SM / Berks BC | |||||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 42.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 21.8 KB 21.8 KB | Display Display | ![]() |
Images | ![]() | 47 KB | ||
Filedesc metadata | ![]() | 9.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 390.8 KB | Display | ![]() |
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Full document | ![]() | 390.4 KB | Display | |
Data in XML | ![]() | 8 KB | Display | |
Data in CIF | ![]() | 9.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8gl8MC ![]() 8gl6C ![]() 8gljC ![]() 8glkC ![]() 8glmC ![]() 8glnC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Composite volume used for refinement of coordinates created from EMD-29911 and EMD-40085 the associated consensus volume is EMD-40086 | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.832 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
+Entire : Type 9 Secretion System Extended Translocon
+Supramolecule #1: Type 9 Secretion System Extended Translocon
+Macromolecule #1: Protein involved in gliding motility SprA
+Macromolecule #2: Peptidyl-prolyl cis-trans isomerase
+Macromolecule #3: Type IX secretion system protein PorV domain-containing protein
+Macromolecule #4: RemZ
+Macromolecule #5: Periplasmic chaperone for outer membrane proteins Skp
+Macromolecule #6: SprE
+Macromolecule #7: Lauryl Maltose Neopentyl Glycol
+Macromolecule #8: alpha-D-mannopyranose
+Macromolecule #9: water
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 61.2 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 546490 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0) |
Final angle assignment | Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0) |