- EMDB-33043: CryoEM structure of dsDNA-RuvB-RuvA domain3 complex -
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Entry
Database: EMDB / ID: EMD-33043
Title
CryoEM structure of dsDNA-RuvB-RuvA domain3 complex
Map data
Sample
Complex: RuvB region of the RuvA-RuvB-Holliday junction complex
Complex: DNA
DNA: DNA
DNA: DNA
Complex: RuvB-RuvA
Protein or peptide: Holliday junction ATP-dependent DNA helicase RuvB
Protein or peptide: Holliday junction ATP-dependent DNA helicase RuvA
Keywords
Holliday juncition / Homologous recombination / DNA damage repair / ATP hydrolysis / MOTOR PROTEIN-DNA COMPLEX
Function / homology
Function and homology information
cellular response to chromate / Holliday junction helicase complex / cellular response to selenite ion / Holliday junction resolvase complex / four-way junction helicase activity / four-way junction DNA binding / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / DNA recombination / DNA repair / ATP hydrolysis activity ...cellular response to chromate / Holliday junction helicase complex / cellular response to selenite ion / Holliday junction resolvase complex / four-way junction helicase activity / four-way junction DNA binding / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / DNA recombination / DNA repair / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function
DNA helicase, Holliday junction RuvB-type / RuvB C-terminal winged helix domain / Holliday junction DNA helicase RuvA, C-terminal / DNA helicase, Holliday junction RuvA type, domain I, bacterial / RuvA, C-terminal domain superfamily / RuvB, AAA lid domain / RuvA N terminal domain / RuvB C-terminal winged helix domain / RuvA, C-terminal domain / RuvB AAA lid domain ...DNA helicase, Holliday junction RuvB-type / RuvB C-terminal winged helix domain / Holliday junction DNA helicase RuvA, C-terminal / DNA helicase, Holliday junction RuvA type, domain I, bacterial / RuvA, C-terminal domain superfamily / RuvB, AAA lid domain / RuvA N terminal domain / RuvB C-terminal winged helix domain / RuvA, C-terminal domain / RuvB AAA lid domain / Bacterial DNA recombination protein RuvA / RuvB-like P-loop domain / Holliday junction DNA helicase RuvB P-loop domain / RuvA domain 2-like / Helix-hairpin-helix domain / Helix-hairpin-helix DNA-binding motif, class 1 / Helix-hairpin-helix DNA-binding motif class 1 / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Nucleic acid-binding, OB-fold / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
National Natural Science Foundation of China (NSFC)
31971222
China
National Natural Science Foundation of China (NSFC)
32171194
China
Citation
Journal: Front Plant Sci / Year: 2023 Title: Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from . Authors: Xu Zhang / Zixuan Zhou / Lin Dai / Yulin Chao / Zheng Liu / Mingdong Huang / Qianhui Qu / Zhonghui Lin / Abstract: Holliday junction (HJ) is a four-way structured DNA intermediate in homologous recombination. In bacteria, the HJ-specific binding protein RuvA and the motor protein RuvB together form the RuvAB ...Holliday junction (HJ) is a four-way structured DNA intermediate in homologous recombination. In bacteria, the HJ-specific binding protein RuvA and the motor protein RuvB together form the RuvAB complex to catalyze HJ branch migration. (, Pa) is a ubiquitous opportunistic bacterial pathogen that can cause serious infection in a variety of host species, including vertebrate animals, insects and plants. Here, we describe the cryo-Electron Microscopy (cryo-EM) structure of the RuvAB-HJ intermediate complex from . The structure shows that two RuvA tetramers sandwich HJ at the junction center and disrupt base pairs at the branch points of RuvB-free HJ arms. Eight RuvB subunits are recruited by the RuvA octameric core and form two open-rings to encircle two opposite HJ arms. Each RuvB subunit individually binds a RuvA domain III. The four RuvB subunits within the ring display distinct subdomain conformations, and two of them engage the central DNA duplex at both strands with their C-terminal β-hairpins. Together with the biochemical analyses, our structure implicates a potential mechanism of RuvB motor assembly onto HJ DNA.
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