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- EMDB-30132: Cryo-EM structure of the cargo-loaded encapsulin from Mycobacteri... -

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Basic information

Entry
Database: EMDB / ID: EMD-30132
TitleCryo-EM structure of the cargo-loaded encapsulin from Mycobacterium smegmatis
Map dataNon-post-processed full map after 3D-refinement generated by RELION
Sample
  • Complex: Encapsulin from Mycobacterium smegmatis
    • Protein or peptide: Cargo-loaded encapsulin
Biological speciesMycolicibacterium smegmatis MC2 155 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.1 Å
AuthorsTang YT / Mu A / Gong HR / Wang Q / Rao ZH
Funding support China, 4 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81520108019 China
National Natural Science Foundation of China (NSFC)813300237 China
Ministry of Science and Technology (MoST, China)2017YFC0840300 China
Chinese Academy of SciencesXDB08020200 China
CitationJournal: Proc Natl Acad Sci U S A / Year: 2021
Title: Cryo-EM structure of DyP-loaded encapsulin.
Authors: Yanting Tang / An Mu / Yuying Zhang / Shan Zhou / Weiwei Wang / Yuezheng Lai / Xiaoting Zhou / Fengjiang Liu / Xiuna Yang / Hongri Gong / Quan Wang / Zihe Rao /
Abstract: Encapsulins containing dye-decolorizing peroxidase (DyP)-type peroxidases are ubiquitous among prokaryotes, protecting cells against oxidative stress. However, little is known about how they interact ...Encapsulins containing dye-decolorizing peroxidase (DyP)-type peroxidases are ubiquitous among prokaryotes, protecting cells against oxidative stress. However, little is known about how they interact and function. Here, we have isolated a native cargo-packaging encapsulin from and determined its complete high-resolution structure by cryogenic electron microscopy (cryo-EM). This encapsulin comprises an icosahedral shell and a dodecameric DyP cargo. The dodecameric DyP consists of two hexamers with a twofold axis of symmetry and stretches across the interior of the encapsulin. Our results reveal that the encapsulin shell plays a role in stabilizing the dodecameric DyP. Furthermore, we have proposed a potential mechanism for removing the hydrogen peroxide based on the structural features. Our study also suggests that the DyP is the primary cargo protein of mycobacterial encapsulins and is a potential target for antituberculosis drug discovery.
History
DepositionMar 19, 2020-
Header (metadata) releaseMar 24, 2021-
Map releaseMar 24, 2021-
UpdateApr 28, 2021-
Current statusApr 28, 2021Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.01
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.01
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_30132.png

Downloads & links

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Map

FileDownload / File: emd_30132.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNon-post-processed full map after 3D-refinement generated by RELION
Voxel sizeX=Y=Z: 1.04 Å
Density
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.01
Minimum - Maximum-0.0100079635 - 0.045825347
Average (Standard dev.)0.00096176413 (±0.0044591893)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 374.4 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.041.041.04
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z374.400374.400374.400
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ342342342
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.0100.0460.001

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Supplemental data

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Mask #1

Fileemd_30132_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: First one of the half map after 3D-refinement generated by RELION

Fileemd_30132_half_map_1.map
AnnotationFirst one of the half map after 3D-refinement generated by RELION
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Second one of the half map after 3D-refinement generated by RELION

Fileemd_30132_half_map_2.map
AnnotationSecond one of the half map after 3D-refinement generated by RELION
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Encapsulin from Mycobacterium smegmatis

EntireName: Encapsulin from Mycobacterium smegmatis
Components
  • Complex: Encapsulin from Mycobacterium smegmatis
    • Protein or peptide: Cargo-loaded encapsulin

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Supramolecule #1: Encapsulin from Mycobacterium smegmatis

SupramoleculeName: Encapsulin from Mycobacterium smegmatis / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)

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Macromolecule #1: Cargo-loaded encapsulin

MacromoleculeName: Cargo-loaded encapsulin / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
SequenceString: MNNLYRDLAP ITESAWAEIE LEATRTFKRH IAGRRVVDVS GPNGPTTASV STGHLLDVSP PGDGVIAHLR DAKPLVRLRV PFTVARRDID DVERGSQDSD WDPVKDAAKK LAFVEDRAIF EGYAAASIEG IRSSSSNPAL ALPDDAREIP DVIAQALSEL RLAGVDGPYS ...String:
MNNLYRDLAP ITESAWAEIE LEATRTFKRH IAGRRVVDVS GPNGPTTASV STGHLLDVSP PGDGVIAHLR DAKPLVRLRV PFTVARRDID DVERGSQDSD WDPVKDAAKK LAFVEDRAIF EGYAAASIEG IRSSSSNPAL ALPDDAREIP DVIAQALSEL RLAGVDGPYS VLLSAETYTK VSETTAHGYP IREHINRLVD GEIIWAPAID GAFVLSTRGG DFDLQLGTDV SIGYLSHDAE VVHLYMEETM TFLCYTAEAS VALTP MPAP QPQPVLAPLT PAAVFLVATI DEGQEATVYD ALPDISGLVR AIGFRDPAKR LSAITSIGSD AWDRLFSGPR PAELHPFREI DGGRHHAPAT PGDLLFHLRA ESMDVCFELA TKLVEAMSGA ITIVDETHGF RFFDNRDLMG FVDGTENPDG NLAVVATQIG DEDPDFAGGC YVHVQKYLHD MASWNSLSVE EQERVIGRTK LDDIELDDDV KPANSHVALN VIEDEDGNEL KIIRHNMPFG EIGKGEFGTY YIGYSRTPSV TERMLDNMFI GDPPGNTDRI LDFSTAITGG LFFTPTVDFL DDPPPLPSED DRAEPASAPS ADPVHTDGSL GIGSLKGTR

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration10 mg/mL
BufferpH: 7.4
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: OTHER
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Frames/image: 1-40 / Average exposure time: 6.4 sec. / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: Gctf
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION
Details: The primary map was reconstructed from 3D refinement job in RELION, which was not post-processed. Due to the flexibility between encapsulin shell and cargo proteins inside, post-processing ...Details: The primary map was reconstructed from 3D refinement job in RELION, which was not post-processed. Due to the flexibility between encapsulin shell and cargo proteins inside, post-processing job would make cargo proteins part of the final map very noisy. So the full map directly from 3D refinement job was considered as the primary map.
Number images used: 25020
FSC plot (resolution estimation)

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