EMDB-29273: Human nuclear pre-60S ribosomal subunit (State K2) - Composite map

Basic information

Entry

Database: EMDB / ID: EMD-29273

• Title: Human nuclear pre-60S ribosomal subunit (State K2) - Composite map
• Map data: Human nuclear pre-60S ribosomal subunit (State K2)

Sample

• Complex: Human nuclear pre-60S ribosomal subunit (State K2)
  • Protein or peptide: x 51 types
  • RNA: x 3 types
• Ligand: x 4 types

• Keywords: Pre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex

Function / homology

Function:

protein localization to nucleoplasm / negative regulation of transcription of nucleolar large rRNA by RNA polymerase I / negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding / regulation of RIG-I signaling pathway / basal RNA polymerase II transcription machinery binding / negative regulation of collagen binding / dendrite extension / preribosome binding / lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / PeBoW complex / miRNA-mediated post-transcriptional gene silencing / negative regulation of signal transduction by p53 class mediator / miRNA-mediated gene silencing by inhibition of translation / eukaryotic 80S initiation complex / negative regulation of protein neddylation / translation at presynapse / axial mesoderm development / ribosomal protein import into nucleus / negative regulation of formation of translation preinitiation complex / 90S preribosome assembly / blastocyst formation / positive regulation of protein K63-linked deubiquitination / protein localization to nucleolus / TORC2 complex binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / GAIT complex / middle ear morphogenesis / regulation of glycolytic process / A band / regulation of reactive oxygen species metabolic process / alpha-beta T cell differentiation / cytoplasmic side of rough endoplasmic reticulum membrane / regulation of G1 to G0 transition / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / negative regulation of ubiquitin protein ligase activity / regulation of aerobic respiration / maturation of 5.8S rRNA / response to aldosterone / regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of cell-cell adhesion / positive regulation of dendritic spine development / homeostatic process / rRNA metabolic process / G1 to G0 transition / negative regulation of DNA replication / macrophage chemotaxis / lung morphogenesis / Protein hydroxylation / mitotic G2 DNA damage checkpoint signaling / ribosomal large subunit binding / Peptide chain elongation / rRNA transcription / preribosome, large subunit precursor / nuclear-transcribed mRNA catabolic process / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / blastocyst development / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / protein localization to nucleus / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / protein targeting / negative regulation of protein-containing complex assembly / protein-RNA complex assembly / cellular response to interleukin-4 / cellular response to actinomycin D / ribonucleoprotein complex binding / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of ubiquitin-dependent protein catabolic process / ribosomal subunit export from nucleus / rough endoplasmic reticulum / MDM2/MDM4 family protein binding / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / maturation of LSU-rRNA / negative regulation of protein ubiquitination / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cytosolic ribosome / embryo implantation / translation initiation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ossification / assembly of large subunit precursor of preribosome / ribosomal large subunit biogenesis / negative regulation of cell migration / cytosolic ribosome assembly / regulation of signal transduction by p53 class mediator / innate immune response in mucosa

Domain/homology:

Uncharacterised protein family UPF0390 / Protein of unknown function (DUF2462) / Translation machinery-associated protein 16 / Tma16 superfamily / Translation machinery-associated protein 16 / MKI67 FHA domain-interacting nucleolar phosphoprotein, FHA Ki67 binding / FHA Ki67 binding domain of hNIFK / Learning associated protein 18-like / LLP homolog / Ribosome biogenesis protein Nop53/GLTSCR2 / Nop53 (60S ribosomal biogenesis) / : / Zinc-finger double-stranded RNA-binding / Zinc finger, double-stranded RNA binding / Pescadillo / Pescadillo N-terminus / Ribosomal protein L24e / Ribosome assembly factor Mrt4 / : / BRCT domain, a BRCA1 C-terminus domain / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / GTP binding domain / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal protein L28e / Ribosomal L15/L27a, N-terminal / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / breast cancer carboxy-terminal domain / metallochaperone-like domain / TRASH domain / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Ribosomal protein L19, eukaryotic / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L44e / Ribosomal protein L24e, conserved site / Ribosomal protein L44 / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L30e signature 1. / Ribosomal protein L6e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L30e, conserved site / Ribosomal protein L36e signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Zinc finger C2H2 type domain profile. / Ribosomal protein L35Ae, conserved site

Component:

Large ribosomal subunit protein eL42 / Zinc finger protein 593 / Pescadillo homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL14 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Ribosomal protein uL30-like / Translation machinery-associated protein 16 / Protein LLP homolog / MKI67 FHA domain-interacting nucleolar phosphoprotein / GTP-binding protein 4 / Ribosome biogenesis protein NOP53 / Probable ribosome biogenesis protein RLP24 / mRNA turnover protein 4 homolog / Leydig cell tumor 10 kDa protein homolog / Large ribosomal subunit protein eL36

• Biological species: Homo sapiens (human) / human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 2.55 Å
• Authors: Vanden Broeck A / Klinge S

Funding support

European Union, United States, 2 items

Organization	Grant number	Country
European Molecular Biology Organization (EMBO)	ALTF 711-2019	European Union
The G. Harold and Leila Y. Mathers Foundation	MF-2104-01554	United States

• Citation: Journal: Science / Year: 2023; Title: Principles of human pre-60 biogenesis.; Authors: Arnaud Vanden Broeck / Sebastian Klinge / ; Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

History

Deposition	Dec 21, 2022	-
Header (metadata) release	Jul 12, 2023	-
Map release	Jul 12, 2023	-
Update	Jul 26, 2023	-
Current status	Jul 26, 2023	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_29273.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Human nuclear pre-60S ribosomal subunit (State K2)
• Voxel size: X=Y=Z: 1.072 Å

Density

Contour Level	By AUTHOR: 0.85
Minimum - Maximum	-0.0034550256 - 10.876910000000001
Average (Standard dev.)	0.05525214 (±0.19372961)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 514.56 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_29273_msk_1.map

Half map: Half Map 1

• File: emd_29273_half_map_1.map
• Annotation: Half Map 1

Half map: Half Map 2

• File: emd_29273_half_map_2.map
• Annotation: Half Map 2

Sample components

Entire : Human nuclear pre-60S ribosomal subunit (State K2)

• Entire: Name: Human nuclear pre-60S ribosomal subunit (State K2)

Components

• Complex: Human nuclear pre-60S ribosomal subunit (State K2)
  • Protein or peptide: 60S ribosomal protein L12
  • RNA: 5.8S rRNA
  • RNA: 28S rRNA
  • RNA: 5S rRNA
  • Protein or peptide: 60S ribosomal protein L11
  • Protein or peptide: 60S ribosomal protein L13
  • Protein or peptide: 60S ribosomal protein L13a
  • Protein or peptide: 60S ribosomal protein L14
  • Protein or peptide: 60S ribosomal protein L15
  • Protein or peptide: 60S ribosomal protein L17
  • Protein or peptide: 60S ribosomal protein L18
  • Protein or peptide: 60S ribosomal protein L18a
  • Protein or peptide: 60S ribosomal protein L19
  • Protein or peptide: 60S ribosomal protein L21
  • Protein or peptide: 60S ribosomal protein L22
  • Protein or peptide: 60S ribosomal protein L23
  • Protein or peptide: 60S ribosomal protein L23a
  • Protein or peptide: 60S ribosomal protein L26
  • Protein or peptide: 60S ribosomal protein L27
  • Protein or peptide: 60S ribosomal protein L27a
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L34
  • Protein or peptide: 60S ribosomal protein L35
  • Protein or peptide: 60S ribosomal protein L35a
  • Protein or peptide: 60S ribosomal protein L36
  • Protein or peptide: 60S ribosomal protein L36a
  • Protein or peptide: 60S ribosomal protein L37
  • Protein or peptide: 60S ribosomal protein L37a
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: Protein LLP homolog
  • Protein or peptide: Ribosome biogenesis protein NOP53
  • Protein or peptide: Zinc finger protein 593
  • Protein or peptide: Translation machinery-associated protein 16
  • Protein or peptide: 60S ribosomal protein L4
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6
  • Protein or peptide: 60S ribosomal protein L7
  • Protein or peptide: 60S ribosomal protein L7a
  • Protein or peptide: 60S ribosomal protein L8
  • Protein or peptide: 60S ribosomal protein L9
  • Protein or peptide: MKI67 FHA domain-interacting nucleolar phosphoprotein
  • Protein or peptide: 60S ribosomal protein L7-like 1
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • Protein or peptide: Pescadillo homolog
  • Protein or peptide: mRNA turnover protein 4 homolog
  • Protein or peptide: GTP-binding protein 4
  • Protein or peptide: Probable ribosome biogenesis protein RLP24
  • Protein or peptide: Leydig cell tumor 10 kDa protein homolog
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: POTASSIUM ION

Supramolecule #1: Human nuclear pre-60S ribosomal subunit (State K2)

• Supramolecule: Name: Human nuclear pre-60S ribosomal subunit (State K2) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#54
• Source (natural): Organism: Homo sapiens (human) / Strain: HEK293F

Macromolecule #1: 60S ribosomal protein L12

• Macromolecule: Name: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #5: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #6: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.321682 KDa

Sequence

String:

MAPSRNGMVL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPVRN V YKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #7: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.633412 KDa

Sequence

String:

MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #8: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.485016 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #9: 60S ribosomal protein L15

• Macromolecule: Name: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #10: 60S ribosomal protein L17

• Macromolecule: Name: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.44317 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLQKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #11: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.687676 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

Macromolecule #12: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 20.808514 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #13: 60S ribosomal protein L19

• Macromolecule: Name: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

Macromolecule #14: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #15: 60S ribosomal protein L22

• Macromolecule: Name: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.813015 KDa

Sequence

String:

MAPVKKLVVK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

UniProtKB: Large ribosomal subunit protein eL22

Macromolecule #16: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #17: 60S ribosomal protein L23a

• Macromolecule: Name: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.740193 KDa

Sequence

String:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #18: 60S ribosomal protein L26

• Macromolecule: Name: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #19: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

Macromolecule #20: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 16.604535 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGLHH HRINFDKYHP GYFGKVGMKH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKS VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #21: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.784622 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #22: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.804275 KDa

Sequence

String:

MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMSARAE AIKALVKPKE VKPKIPKGV SRKLDRLAYI AHPKLGKRAR ARIAKGLRLC RPKAKAKAKA KDQTKAQAAA PASVPAQAPK RTQAPTKASE

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #23: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 46.224133 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #24: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.805092 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLAIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #25: 60S ribosomal protein L31

• Macromolecule: Name: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #26: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #27: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 13.326074 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #28: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.593624 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ENLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #29: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.564743 KDa

Sequence

String:

MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #30: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.290859 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #31: 60S ribosomal protein L36a

• Macromolecule: Name: 60S ribosomal protein L36a / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.476973 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Large ribosomal subunit protein eL42

Macromolecule #32: 60S ribosomal protein L37

• Macromolecule: Name: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #33: 60S ribosomal protein L37a

• Macromolecule: Name: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 10.29935 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

Macromolecule #34: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 8.238948 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #35: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 6.426759 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIRMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #36: Protein LLP homolog

• Macromolecule: Name: Protein LLP homolog / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.268361 KDa

Sequence

String:

MAKSLRSKWK RKMRAEKRKK NAPKEASRLK SILKLDGDVL MKDVQEIATV VVPKPKHCQE KMQCEVKDEK DDMKMETDIK RNKKTLLDQ HGQYPIWMNQ RQRKRLKAKR EKRKGKSKAK AVKVAKGLAW

UniProtKB: Protein LLP homolog

Macromolecule #37: Ribosome biogenesis protein NOP53

• Macromolecule: Name: Ribosome biogenesis protein NOP53 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 54.498402 KDa

Sequence

String:

MAAGGSGVGG KRSSKSDADS GFLGLRPTSV DPALRRRRRG PRNKKRGWRR LAQEPLGLEV DQFLEDVRLQ ERTSGGLLSE APNEKLFFV DTGSKEKGLT KKRTKVQKKS LLLKKPLRVD LILENTSKVP APKDVLAHQV PNAKKLRRKE QLWEKLAKQG E LPREVRRA QARLLNPSAT RAKPGPQDTV ERPFYDLWAS DNPLDRPLVG QDEFFLEQTK KKGVKRPARL HTKPSQAPAV EV APAGASY NPSFEDHQTL LSAAHEVELQ RQKEAEKLER QLALPATEQA ATQESTFQEL CEGLLEESDG EGEPGQGEGP EAG DAEVCP TPARLATTEK KTEQQRRREK AVHRLRVQQA ALRAARLRHQ ELFRLRGIKA QVALRLAELA RRQRRRQARR EAEA DKPRR LGRLKYQAPD IDVQLSSELT DSLRTLKPEG NILRDRFKSF QRRNMIEPRE RAKFKRKYKV KLVEKRAFRE IQL

UniProtKB: Ribosome biogenesis protein NOP53

Macromolecule #38: Zinc finger protein 593

• Macromolecule: Name: Zinc finger protein 593 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.230225 KDa

Sequence

String:

MGRSRRTGAH RAHSLARQMK AKRRRPDLDE IHRELRPQGS ARPQPDPNAE FDPDLPGGGL HRCLACARYF IDSTNLKTHF RSKDHKKRL KQLSVEPYSQ EEAERAAGMG SYVPPRRLAV PTEVSTEVPE MDTST

UniProtKB: Zinc finger protein 593

Macromolecule #39: Translation machinery-associated protein 16

• Macromolecule: Name: Translation machinery-associated protein 16 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.992393 KDa

Sequence

String:

MPKAPKGKSA GREKKVIHPY SRKAAQITRE AHKQEKKEKL KNEKALRLNL VGEKLQWFQN HLDPQKKRYS KKDACELIER YLNRFSSEL EQIELHNSIR DRQGRRHCSR ETVIKQTMER ERQQFEG(PTR)GL EIPDILNASN LKTFREWDFD LKKLPNI KM RKICANDAIP KTCKRKTIIT VDQDLGELEL NDESSDSDEE MTAVA

UniProtKB: Translation machinery-associated protein 16

Macromolecule #40: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 47.804621 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #41: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.426789 KDa

Sequence

String:

MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NSVTPDMMEE MY KKAHAAI RENPVYEKKP KKEVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #42: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 32.810176 KDa

Sequence

String:

MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #43: 60S ribosomal protein L7

• Macromolecule: Name: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.290973 KDa

Sequence

String:

MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #44: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #45: 60S ribosomal protein L8

• Macromolecule: Name: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 28.088863 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GTKTVQEKEN

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #46: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.899471 KDa

Sequence

String:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #47: MKI67 FHA domain-interacting nucleolar phosphoprotein

• Macromolecule: Name: MKI67 FHA domain-interacting nucleolar phosphoprotein / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.285309 KDa

Sequence

String:

MATFSGPAGP ILSLNPQEDV EFQKEVAQVR KRITQRKKQE QLTPGVVYVR HLPNLLDETQ IFSYFSQFGT VTRFRLSRSK RTGNSKGYA FVEFESEDVA KIVAETMNNY LFGERLLECH FMPPEKVHKE LFKDWNIPFK QPSYPSVKRY NRNRTLTQKL R MEERFKKK ERLLRKKLAK KGIDYDFPSL ILQKTESISK TNRQTSTKGQ VLRKKKKKVS GTLDTPEKTV DSQGPTPVCT PT FLERRKS QVAELNDDDK DDEIVFKQPI SCVKEEIQET QTPTHSRKKR RRSSNQ

UniProtKB: MKI67 FHA domain-interacting nucleolar phosphoprotein

Macromolecule #48: 60S ribosomal protein L7-like 1

• Macromolecule: Name: 60S ribosomal protein L7-like 1 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.730354 KDa

Sequence

String:

MISSCTTRKM AEQEQRKIPL VPENLLKKRK AYQALKATQA KQALLAKKEQ KKGKGLRFKR LESFLHDSWR QKRDKVRLRR LEVKPHALE LPDKHSLAFV VRIERIDGVS LLVQRTIARL RLKKIFSGVF VKVTPQNLKM LRIVEPYVTW GFPNLKSVRE L ILKRGQAK VKNKTIPLTD NTVIEEHLGK FGVICLEDLI HEIAFPGKHF QEISWFLCPF HLSVARHATK NRVGFLKEMG TP GYRGERI NQLIRQLN

UniProtKB: Ribosomal protein uL30-like

Macromolecule #49: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 26.62001 KDa

Sequence

String:

MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE DQDELSSLLQ VPLVAGTVNR GSEVIAAGMV VNDWCAFCGL DTTSTELSVV ESVFKLNEAQ PSTIATSMRD SL IDSLT

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #50: Pescadillo homolog

• Macromolecule: Name: Pescadillo homolog / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 68.114727 KDa

Sequence

String:

MGGLEKKKYE RGSATNYITR NKARKKLQLS LADFRRLCIL KGIYPHEPKH KKKVNKGSTA ARTFYLIKDI RFLLHEPIVN KFREYKVFV RKLRKAYGKS EWNTVERLKD NKPNYKLDHI IKERYPTFID ALRDLDDALS MCFLFSTFPR TGKCHVQTIQ L CRRLTVEF MHYIIAARAL RKVFLSIKGI YYQAEVLGQP IVWITPYAFS HDHPTDVDYR VMATFTEFYT TLLGFVNFRL YQ LLNLHYP PKLEGQAQAE AKAGEGTYAL DSESCMEKLA ALSASLARVV VPATEEEAEV DEFPTDGEMS AQEEDRRKEL EAQ EKHKKL FEGLKFFLNR EVPREALAFI IRSFGGEVSW DKSLCIGATY DVTDSRITHQ IVDRPGQQTS VIGRCYVQPQ WVFD SVNAR LLLPVAEYFS GVQLPPHLSP FVTEKEGDYV PPEKLKLLAL QRGEDPGNLN ESEEEEEEDD NNEGDGDEEG ENEEE EEDA EAGSEKEEEA RLAALEEQRM EGKKPRVMAG TLKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG KRRKIR EAN KLAEKRKAHD EAVRSEKKAK KARPE

UniProtKB: Pescadillo homolog

Macromolecule #51: mRNA turnover protein 4 homolog

• Macromolecule: Name: mRNA turnover protein 4 homolog / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 27.602535 KDa

Sequence

String:

MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT LLSDYEVCKE GDVLTPEQAR VLKLFGYEMA EFKVTIKYMW DSQSGRFQQM GDDLPESASE STEESDSEDD D

UniProtKB: mRNA turnover protein 4 homolog

Macromolecule #52: GTP-binding protein 4

• Macromolecule: Name: GTP-binding protein 4 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 74.10782 KDa

Sequence

String:

MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT RTLLLCGYPN VGKSSFINKV TRADVDVQPY AFTTKSLFVG HMDYKYLRWQ VVDTPGILDH PLEDRNTIEM QA ITALAHL RAAVLYVMDL SEQCGHGLRE QLELFQNIRP LFINKPLIVV ANKCDVKRIA ELSEDDQKIF TDLQSEGFPV IET STLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERD LELEM GDDYILDLQK YWDLMNLSEK HDKIPEIWEG HNIADYIDPA IMKKLEELEK EEELRTAAGE YDSVSESEDE EMLEI RQLA KQIREKKKLK ILESKEKNTQ GPRMPRTAKK VQRTVLEKEM RSLGVDMDDK DDAHYAVQAR RSRSITRKRK REDSAP PSS VARSGSCSRT PRDVSGLRDV KMVKKAKTMM KNAQKKMNRL GKKGEADRHV FDMKPKHLLS GKRKAGKKDR R

UniProtKB: GTP-binding protein 4

Macromolecule #53: Probable ribosome biogenesis protein RLP24

• Macromolecule: Name: Probable ribosome biogenesis protein RLP24 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 19.666258 KDa

Sequence

String:

MRIEKCYFCS GPIYPGHGMM FVRNDCKVFR FCKSKCHKNF KKKRNPRKVR WTKAFRKAAG KELTVDNSFE FEKRRNEPIK YQRELWNKT IDAMKRVEEI KQKRQAKFIM NRLKKNKELQ KVQDIKEVKQ NIHLIRAPLA GKGKQLEEKM VQQLQEDVDM E DAP

UniProtKB: Probable ribosome biogenesis protein RLP24

Macromolecule #54: Leydig cell tumor 10 kDa protein homolog

• Macromolecule: Name: Leydig cell tumor 10 kDa protein homolog / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 10.605679 KDa

Sequence

String:

MAQGQRKFQA HKPAKSKTAA AASEKNRGPR KGGRVIAPKK ARVVQQQKLK KNLEVGIRKK IEHDVVMKAS SSLPKKLALL KAPAKKKGA AAATSSKTPS

UniProtKB: Leydig cell tumor 10 kDa protein homolog

Macromolecule #2: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 50.46384 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGC(PSU)GCGAG AAUUAAUG(PSU)G AA UU(OMG)CAGG ACACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGA GCGUCGCUU

GENBANK: GENBANK: U13369.1

Macromolecule #3: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 1.6412037499999999 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA G (A2M)G(A2M)GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUC AACCC GGCGGCGGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGCC CUCC CUUCCCCCGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCCG GGG GUGGGGUCGG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGC GA CCGGCUCCGG GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCCU C CUCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUG GCGGCGGCGG CGGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCCG GGGCCGAGG GAGCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG G UCUCCCCC GCGGGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC CC CUCCUCC CCGCGCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GCG CCCCGG GCGGGUCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCGG AGCGA GCGCACGGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC C(OMG)UCUUG(1MA)AA C(A2M)CGGACCAA GGA GU(OMC)UAA CACGUGCGCG AGUCGGGGGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCGCUCGC CGGCCGAGG UGGGAUCCCG AGGCCUCUCC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG G UGGAGCAC GAGCGCACGU GUUAGGACCC (OMG)A(A2M)AGAUGGU GA(A2M)C(PSU)AUGCC UGGGCAGGGC GAAGCC AGA GGAAACUCUG GUGGAGGUCC G(PSU)AGCGGUCC UGACGUGCAA AUCGGUCGUC CGACCUGGGU AUAG(OMG)GGCG AAAGACUAAU CGAACCAUCU AGUAGCUGGU UCCCUCCGAA GUUUCCC(PSU)CA GGA(PSU)AGCUGG CGCUCUCGCA GA CCCGACG CACCCCCGCC ACGCAGUUUU AUCCGGUAAA GCGAA(PSU)GAUU AGAGGUCUUG G(OMG)GCCGAAAC GAUC (OMU)CAAC C(PSU)A(PSU)(PSU)CUCAA ACUU(PSU)AAAUG GGUAAGAAGC CCGGCUCGCU GGCGUGGAGC CGGGC GUGG AAUGCGAGUG CCUAGUGGGC CAC(PSU)U(PSU)UGGU AAGC(A2M)GAACU GGCGCUGCGG GAUGAACCGA ACGC CGGGU UAAGGCGCCC GAUGCCGACG CUCAUCAGAC CCCAGAAAAG GUGUUGGUUG AUAUAGACAG CAGGACGGUG GCCAU GGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACCUGCCG AAUCAACUAG CCCUGAAAAU GGAUGGCGCU GGAGCG UCG GGCCCAUACC CGGCCGUCGC CGGCAGUCGA GAGUGGACGG GAGCGGCGGG GGCGGCGCGC GCGCGCGCGC GUGUGGU GU GCGUCGGAGG GCGGCGGCGG CGGCGGCGGC GGGGGUGUGG GGUCCUUCCC CCGCCCCCCC CCCCACGCCU CCUCCCCU C CUCCCGCCCA CGCCCCGCUC CCCGCCCCCG GAGCCCCGCG GACGCUACGC CGCGACGAGU AGGAGGGCCG CUGCGGUGA GCCUUGAAGC CUAGGGCGCG GGCCCGGGUG GAGCCGCCGC AGGUGCAGAU (OMC)UUGGUGGUA GU(A2M)(OMG) (OMC)AAAU AUUCAAACGA GAACUUUGAA GGCCGAAGUG G(A2M)GAAGGGUU CCAUG(OMU)GAAC AG(OMC)A(OMG) UUGA ACAUGGGUCA GUCGGUCCUG AGAGAUGGGC GAGCGCCGUU CCGAAGGGAC GGGCGAUGGC CUCCGUUGCC CUCGGC CGA (PSU)CGAAAGGGA GUCGGGUUCA GAUCCCCGAA UCCGGAGUGG CGGAGAUGGG CGCCGCGAGG CGUCCAGUGC GGU AACGCG ACCGAUCCCG GAGAAGCCGG CGGGAGCCCC GGGGAGAGUU CUCUU(PSU)UCUU UGUGAAGGGC AGGGCGCCCU GGAAUGGGU UCGCCCCGAG AGAGGGGCCC GUGCCUUGGA AAGCGUCGCG GUUCCGGCGG CGUCCGGUGA GCUCUCGCUG G CCCUUGAA AAUCCGGGGG AGAGGGUGUA AAUCUCGCGC CGGGCCGUAC CC(A2M)UAUCCGC AGCAGGUCU(OMC) CAAG GUGAA C(A2M)GCCUCUGG (OMC)AUGUUGGAA CAA(OMU)G(PSU)AGGU AAGGGAAGUC GGCAAGC(OMC)GG AUCCG UAAC UUC(OMG)GGAUAA GGAUUGGCUC UAAGGGCUGG GUCGGUCGGG CUGGGGCGCG AAGCGGGGCU GGGCGCGCGC CG CGGCUGG ACGAGGCGCC GCCGCCCCCC CCACGCCCGG GGCACCCCCC UCGCGGCCCU CCCCCGCCCC ACCCCGCGCG CGC CGCUCG CUCCCUCCCC GCCCCGCGCC CUCUCUCUCU CUCUCUCCCC CGCUCCCCGU CCUCCCCCCU CCCCGGGGGA GCGC CGCGU GGGGGCGGCG GCGGGGGGAG AAGGGUCGGG GCGGCAGGGG CCGGCGGCGG CCCGCCGCGG GGCCCCGGCG GCGGG GGCA CGGUCCCCCG CGAGGGGGGC CCGGGCACCC GGGGGGCCGG CGGCGGCGGC GACUCUGGAC GCGAGCCGGG CCCUUC CCG UGGAUCGCCC CAGCUGCGGC GGGCGUCGCG GCCGCCCCCG GGGAGCCCGG CGGGCGCCGG CGCGCCCCCC CCCCCAC CC CACGUCUCGU CGCGCGCGCG UCCGCUGGGG GCGGGGAGCG GUCGGGCGGC GGCGGUCGGC GGGCGGCGGG GCGGGGCG G UUCGUCCCCC CGCCCUACCC CCCCGGCCCC GUCCGCCCCC CGUUCCCCCC UCCUCCUCGG CGCGCGGCGG CGGCGGCGG CAGGCGGCGG AGGGGCCGCG GGCCGGUCCC CCCCGCCGGG UCCGCCCCCG GGGCCGCGGU UCCGCGCGGC GCCUCGCCUC GGCCGGCGC CUAGCAGCCG ACUUAGAACU GGUGCGGACC AGG(OMG)GAAUCC GAC(PSU)G(PSU)UUAA UUAAAACAA AGCAUCGCGA AGGCCCGCGG CGGGUGUUGA CGCGAUGUGA UU(PSU)CUGCC(OMC)A GUGCUCUGAA UG(PSU)CA (A2M)AGU GA(A2M)GAAAU(PSU)C AA(PSU)GAAGCGC GG(OMG)UAAACGG CGGGAG(PSU)A(A2M)C (PSU)A (PSU)GAC(PSU)C(PSU) CUUAAGGUAG C(5MC)AA(A2M)UGCCU C(OMG)UCAUCUAA UUAGUGA(OMC)GC GCAUG AA(OMU)G GA(PSU)GA(A2M)CGAG (A2M)UUCCCACUG U(OMC)CC(PSU)ACCUA C(PSU)A(PSU)CCAGCG AAAC CAC(A2M)G (OMC)CAAGGGAAC GGGCU(PSU)GG(OMC)G GAAUCAGCGG (OMG)GAAAGAAGA CCCUGUUGAG C (PSU)UGAC(OMU)CU AGUCUGGCAC GGUGAAGAGA CAUGAGAGGU G(PSU)AGAAUAAG UGGGAGGCCC CCGGCGCCCC CCCGGUGUC CCCGCGAGGG GCCCGGGGCG GGGUCCGCCG GCCCUGCGGG CCGCC(OMG)GUGA AAUACCA(OMC)UA CUC UGAUCG UUUUUUCACU GACCCGGUGA GGCGGGGGGG CGAGCCCCGA GGGGCUCUCG CUUCUGGCGC CAAGCGCCCG GCCG CGCGC CGGCCGGGCG CGACCCGCUC CGGGGACAGU GCCAGGUGGG GAGUUUGACU G(OMG)GGCGGUAC ACCUGUCAAA C GGUA(6MZ)CGC AGG(OMU)(OMG)UCCUA AGGCGAGCUC AGGGAGGACA GAAACCUCCC GUGGAGCAGA AGGGCAAAAG CUCGCUUGA (PSU)CU(PSU)GA(PSU)UUU CAG(OMU)ACGAA(PSU) ACAGACCGUG AAAGCGGGGC CUCACGAUCC U UCUGACCU U(PSU)UGGGUUU(PSU) AAGCAGGA(OMG)G UGUCAGAAAA GUUACCACAG (OMG)GAUAACUGG C(PSU) UGUGGCG GCCAAGCGU(PSU) CA(PSU)AGCGACG (PSU)CGCUUUUUG A(PSU)CCUU(5MC)GAU GUCGG(OMC) (PSU)CU UCCUAUCAUU G(PSU)GAAGCAGA AUUCACCAAG CGU(PSU)(OMG)GAU(OMU)(OMG) (PSU)UCACCCAC UAAUAGGGAA CG(PSU)G(A2M)GCUGG G(UR3)U(PSU)AGA(OMC)CG UCGUGAGACA GGU(PSU)AGUUUU ACCCUA CUG AUG(A2M)UGUG(PSU)U G(PSU)UGCCAUGG UA(A2M)UCCUGCU CAGUACGAGA GGAACCGCAG (OMG)U(OMU) CA(OMG)ACA U(PSU)UGGUGUA(PSU) (OMG)UGCUUGGCU GAGGAGCCAA UGGGGCGAAG CUACCA(PSU)CUG UGGG AUUAU GAC(PSU)GAACGC CUCUAAGUCA GAAUCCCGCC CAGGCGGAAC GAUACGGCAG CGCCGCGGAG CCUCGGUUGG C CUCGGAUA GCCGGUCCCC CGCCUGUCCC CGCCGGCGGG CCGCCCCCCC CCUCCACGCG CCCCGCGCGC GCGGGAGGGC GC GUGCCCC GCCGCGCGCC GGGACCGGGG UCCGGUGCGG AGUGCCCUUC GUCCUGGGAA ACGGGGCGCG GCCGGAGAGG CGG CCGCCC CCUCGCCCGU CACGCACCGC ACGUUCGUGG GGAACCUGGC GCUAAACCA(PSU) UCGUAGACGA CCUGCUUCUG GGUCGGGG(PSU) UUCGUACG(PSU)A GCAGAGCAGC UCCCUCGCUG CGAUCUAUUG AAAGUCAGCC CUCGACACAA GGG UUUGUC

GENBANK: GENBANK: AL592188.60

Macromolecule #4: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 38.998078 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU UU

GENBANK: GENBANK: X12811.1

Macromolecule #55: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 55 / Number of copies: 88 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #56: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 56 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #57: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 57 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #58: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 58 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Grid: Model: Quantifoil R3.5/1 / Material: GOLD / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV; Details: Four applications with manual blotting before last blotting with the vitrobot..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Specialist optics: Energy filter - Slit width: 20 eV
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 172699 / Average exposure time: 2.0 sec. / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 15679142
• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.55 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.2) / Number images used: 66157
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.2)
• Final 3D classification: Software: (Name: RELION (ver. 4.0), cryoSPARC (ver. 3.3.2))

Atomic model buiding 1

• Refinement: Space: REAL

Output model

PDB-8flb:
Human nuclear pre-60S ribosomal subunit (State K2)