EMDB-24307: Structure of the translating Neurospora crassa ribosome arrested ...

Basic information

• Entry: Database: EMDB / ID: EMD-24307
• Title: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide

Sample

• Complex: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide
  • Complex: 60S ribosomal subunit
    • RNA: x 3 types
    • Protein or peptide: x 42 types
  • Complex: 40S ribosomal subunit
    • RNA: x 1 types
    • Protein or peptide: x 33 types
  • Complex: tRNA
    • RNA: x 1 types
  • Complex: Nascent peptide
    • Protein or peptide: x 1 types
  • Complex: mRNA
    • RNA: x 1 types
• Ligand: x 3 types

• Keywords: Protein Synthesis Inhibitor / Cytosolic Ribosome / Polysomal Ribosome / Peptidyl Transferase Center / TRANSLATION

Function / homology

Function:

negative regulation of cell integrity MAPK cascade / positive regulation of conjugation with cellular fusion / GCN2-mediated signaling / regulation of cytoplasmic translation / negative regulation of p38MAPK cascade / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / positive regulation of translational fidelity / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / pre-mRNA 5'-splice site binding / preribosome, small subunit precursor / mRNA destabilization / negative regulation of mRNA splicing, via spliceosome / G-protein alpha-subunit binding / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / ribosomal subunit export from nucleus / ribosomal small subunit export from nucleus / positive regulation of autophagy / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) endonuclease activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosome biogenesis / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol / cytoplasm

Domain/homology:

Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L18e / Ribosomal protein 60S L18 and 50S L18e / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein L22e / Ribosomal protein L22e superfamily / S27a-like superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Ribosomal protein L10e, conserved site / Plectin/S10 domain / Ribosomal protein L10e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L19, eukaryotic / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / : / Ribosomal protein S7e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein L30e signature 1. / Ribosomal protein L6e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal L40e family / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal_L40e / Ribosomal protein L40e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L40e superfamily / 40S ribosomal protein S4 C-terminus / Ribosomal protein L23/L25, N-terminal

Component:

Uncharacterized protein / 60S ribosomal protein L35 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein eS21 / Large ribosomal subunit protein uL15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein RACK1 / Ribosomal protein L26 / 60S ribosomal protein L27 / 40S ribosomal protein S24 / 60S ribosomal protein L22 / 60S ribosomal protein L4-A / 60S ribosomal protein L8 / 40S ribosomal protein S3 / 40S ribosomal protein S12 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / 60S ribosomal protein L44 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein eS27 / 60S ribosomal protein L2 / 60S ribosomal protein L34 / 60s ribosomal protein / 60S ribosomal protein L18 / 40S ribosomal protein S18 / Large ribosomal subunit protein eL32 / 60S ribosomal protein L29 / 60S ribosomal protein L33 / 60S ribosomal protein L43 / Large ribosomal subunit protein eL39 / 40S ribosomal protein S20 / 40S ribosomal protein S2 / Ribosomal protein L19 / Small ribosomal subunit protein eS28 / 60S ribosomal protein L13 / 60S ribosomal protein L10 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein uL30 / 40S ribosomal protein S19 / 60S ribosomal protein L25 / Small ribosomal subunit protein eS25 / 40S ribosomal protein S13 / Large ribosomal subunit protein eL24 / 40S ribosomal protein S9 / Ribosomal protein Srp1 / 40S ribosomal protein S4 / 60S ribosomal protein L36 / Small ribosomal subunit protein uS9 / 40S ribosomal protein S11 / 60S ribosomal protein L9 / Ribosomal protein S12 / 60S ribosomal protein L6 / 60S ribosomal protein L23 / Ribosomal protein L14 / Small ribosomal subunit protein eS1 / Probable 40s ribosomal protein s10-b / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS12 / 60S ribosomal protein L41 / 60S ribosomal protein L16 / 40S ribosomal protein S30 / 60S ribosomal protein L40 / 40S ribosomal protein S6 / 40S ribosomal protein S8 / Ribosomal protein L37 / 60S ribosomal protein L20

• Biological species: Neurospora crassa (fungus)
• Method: single particle reconstruction / cryo EM / Resolution: 2.7 Å
• Authors: Shen L / Su Z

Funding support

United States, 3 items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	R21AI138158	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R35 GM126966	United States
Welch Foundation	A1863	United States

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2021; Title: Structure of the translating ribosome arrested by cycloheximide.; Authors: Lunda Shen / Zhaoming Su / Kailu Yang / Cheng Wu / Thomas Becker / Deborah Bell-Pedersen / Junjie Zhang / Matthew S Sachs / ; Abstract: Ribosomes translate RNA into proteins. The protein synthesis inhibitor cycloheximide (CHX) is widely used to inhibit eukaryotic ribosomes engaged in translation elongation. However, the lack of structural data for actively translating polyribosomes stalled by CHX leaves unanswered the question of which elongation step is inhibited. We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating ribosomes bound with CHX at 2.7-Å resolution. The ribosome structure from this filamentous fungus contains clearly resolved ribosomal protein eL28, like higher eukaryotes but unlike budding yeast, which lacks eL28. Despite some differences in overall structures, the ribosomes from , yeast, and humans all contain a highly conserved CHX binding site. We also sequenced classic CHX-resistant alleles. These mutations, including one at a residue not previously observed to affect CHX resistance in eukaryotes, were in the large subunit proteins uL15 and eL42 that are part of the CHX-binding pocket. In addition to A-site transfer RNA (tRNA), P-site tRNA, messenger RNA, and CHX that are associated with the translating ribosome, spermidine is present near the CHX binding site close to the E site on the large subunit. The tRNAs in the peptidyl transferase center are in the A/A site and the P/P site. The nascent peptide is attached to the A-site tRNA and not to the P-site tRNA. The structural and functional data obtained show that CHX arrests the ribosome in the classical PRE translocation state and does not interfere with A-site reactivity.

History

Deposition	Jun 25, 2021	-
Header (metadata) release	Dec 1, 2021	-
Map release	Dec 1, 2021	-
Update	Jun 5, 2024	-
Current status	Jun 5, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_24307.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.06 Å

Density

Contour Level	By AUTHOR: 0.5 / Movie #1: 0.5
Minimum - Maximum	-2.0006697 - 4.039758
Average (Standard dev.)	0.0019136198 (±0.13177417)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 384 384 384 Spacing 384 384 384
Cell	A=B=C: 407.03998 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.06	1.06	1.06
M x/y/z	384	384	384
origin x/y/z	0.000	0.000	0.000
length x/y/z	407.040	407.040	407.040
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	384	384	384
D min/max/mean	-2.001	4.040	0.002

Supplemental data

Mask #1

• File: emd_24307_msk_1.map

Half map: #2

• File: emd_24307_half_map_1.map

Half map: #1

• File: emd_24307_half_map_2.map

Sample components

Entire : Structure of the translating Neurospora crassa ribosome arrested ...

• Entire: Name: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide

Components

• Complex: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide
  • Complex: 60S ribosomal subunit
    • RNA: 26S rRNA
    • RNA: 5S rRNA
    • RNA: 5.8S rRNA
    • Protein or peptide: 60S ribosomal protein L2
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4-A
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: 60S ribosomal protein L7
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: Ribosomal protein L14
    • Protein or peptide: 60S ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L16
    • Protein or peptide: 60S ribosomal protein L17
    • Protein or peptide: 60S ribosomal protein L18
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L20
    • Protein or peptide: Ribosomal protein Srp1
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: 60S ribosomal protein L23
    • Protein or peptide: 60S ribosomal protein L24
    • Protein or peptide: 60S ribosomal protein L25
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27 (eL27)
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60s ribosomal protein
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L33
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L35
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: 60S ribosomal protein L40
    • Protein or peptide: hypothetical protein NCU16635 (eL41)
    • Protein or peptide: 60S ribosomal protein L44
    • Protein or peptide: 60S ribosomal protein L43
    • Protein or peptide: Ribosomal_L28e domain-containing protein
  • Complex: 40S ribosomal subunit
    • RNA: 18S rRNA
    • Protein or peptide: 40S ribosomal protein S0
    • Protein or peptide: 40S ribosomal protein S1
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: Cytoplasmic ribosomal protein 10
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: 40S ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: Probable 40s ribosomal protein s10-b
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein S14 (uS11)
    • Protein or peptide: Ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S16
    • Protein or peptide: 40S ribosomal protein S17
    • Protein or peptide: 40S ribosomal protein S18
    • Protein or peptide: 40S ribosomal protein S19
    • Protein or peptide: 40S ribosomal protein S20
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: 40S ribosomal protein S22
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 40S ribosomal protein S26E
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: 40S ribosomal protein S29
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
    • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
  • Complex: tRNA
    • RNA: tRNA
  • Complex: Nascent peptide
    • Protein or peptide: Nascent peptide
  • Complex: mRNA
    • RNA: mRNA
• Ligand: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione
• Ligand: SPERMIDINE
• Ligand: MAGNESIUM ION

Supramolecule #1: Structure of the translating Neurospora crassa ribosome arrested ...

• Supramolecule: Name: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#82

Supramolecule #2: 60S ribosomal subunit

• Supramolecule: Name: 60S ribosomal subunit / type: complex / ID: 2 / Parent: 1 ; Macromolecule list: #1, #3, #5, #7, #9, #11, #13, #15, #17, #19, #21, #23, #25, #27, #29, #31, #33, #35, #37, #39, #41, #43, #45, #47, #49, #51, #53, #55, #57, #59, #61, #63, #65, #67, #69-#79
• Source (natural): Organism: Neurospora crassa (fungus)

Supramolecule #3: 40S ribosomal subunit

• Supramolecule: Name: 40S ribosomal subunit / type: complex / ID: 3 / Parent: 1 ; Macromolecule list: #2, #4, #6, #8, #10, #12, #14, #16, #18, #20, #22, #24, #26, #28, #30, #32, #34, #36, #38, #40, #42, #44, #46, #48, #50, #52, #54, #56, #58, #60, #62, #64, #66, #68
• Source (natural): Organism: Neurospora crassa (fungus)

Supramolecule #4: tRNA

• Supramolecule: Name: tRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #80
• Source (natural): Organism: Neurospora crassa (fungus)

Supramolecule #5: Nascent peptide

• Supramolecule: Name: Nascent peptide / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #81
• Source (natural): Organism: Neurospora crassa (fungus)

Supramolecule #6: mRNA

• Supramolecule: Name: mRNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #82
• Source (natural): Organism: Neurospora crassa (fungus)

Macromolecule #1: 26S rRNA

• Macromolecule: Name: 26S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 1.07843175 MDa

Sequence

String:

GGUUGACCUC GGAUCAGGUA GGAAUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACAGGGAUUG CCCUAGUAA CGGCGAGUGA AGCGGCAACA GCUCAAAUUU GAAAUCUGGC UUCGGCCCGA GUUGUAAUUU GUAGAGGAAG C UUUUGGUG AGGCACCUUC UGAGUCCCCU GGAACGGGGC GCCAUAGAGG GUGAGAGCCC CGUAUAGUCG GAUGCCGAUC CA AUGUAAA GCUCCUUCGA CGAGUCGAGU AGUUUGGGAA UGCUGCUCAA AAUGGGAGGU AAAUUUCUUC UAAAGCUAAA UAC CGGCCA GAGACCGAUA GCGCACAAGU AGAGUGAUCG AAAGAUGAAA AGCACUUUGA AAAGAGGGUU AAAUAGCACG UGAA AUUGU UGAAAGGGAA GCGUUUGUGA CCAGACUUGC GCCGUUCCGA UCAUCCGGUG UUCUCACCGG UGCACUCGGG ACGGC UCAG GCCAGCAUCG GUUUUGGCGG GGGGAUAAAG GUCCGGGGAA CGUAGCUCCU CCGGGAGUGU UAUAGCCCCG GGCGUA AUG CCCUCGCCGG GACCGAGGUU CGCGCAUCUG CAAGGAUGCU GGCGUAAUGG UCAUCAACGA CCCGUCUUGA AACACGG AC CAAGGAGUCA AGGUUUUGCG CGAGUGUUUG GGUGUAAAAC CCGCACGCGU AAUGAAAGUG AACGUAGGUG AGAGCUUC G GCGCAUCAUC GACCGAUCCU GAUGUAUUCG GAUGGAUUUG AGUAAGAGCG UUAAGCCUUG GACCCGAAAG AUGGUGAAC UAUGCUUGGA UAGGGUGAAG CCAGAGGAAA CUCUGGUGGA GGCUCGCAGC GGUUCUGACG UGCAAAUCGA UCGUCAAAUC UGAGCAUGG GGGCGAAAGA CUAAUCGAAC CAUCUAGUAG CUGGUUACCG CCGAAGUUUC CCUCAGGAUA GCAGUGUUGU U CUUCAGUU UUAUGAGGUA AAGCGAAUGA UUAGGGACUC GGGGGCGCUU UUUAGCCUUC AUCCAUUCUC AAACUUUAAA UA UGUAAGA AGCCCUUGUU ACUUAAUUGA ACGUGGGCAU UCGAAUGUAC CAACACUAGU GGGCCAUUUU UGGUAAGCAG AAC UGGCGA UGCGGGAUGA ACCGAACGCG GGGUUAAGGU GCCGGAGUGG ACGCUCAUCA GACACCACAA AAGGCGUUAG UACA UCUUG ACAGCAGGAC GGUGGCCAUG GAAGUCGGAA UCCGCUAAGG ACUGUGUAAC AACUCACCUG CCGAAUGUAC UAGCC CUGA AAAUGGAUGG CGCUCAAGCG UCCCACCCAU ACCCCGCCCU CAGGGUAGAA ACGAUGCCCU GAGGAGUAGG CGGCCG UGG AGGUCAGUGA CGAAGCCUAG GGCGUGAGCC CGGGUCGAAC GGCCUCUAGU GCAGAUCUUG GUGGUAGUAG CAAAUAC UU CAAUGAGAAC UUGAAGGACC GAAGUGGGGA AAGGUUCCAU GUGAACAGCG GUUGGACAUG GGUUAGUCGA UCCUAAGC C AUAGGGAAGU UCCGUUUCAA AGGGGCACUU GUGCCCCGUG UGGCGAAAGG GAAGCCGGUU AACAUUCCGG CACCUGGAU GUGGGUUUUG CGCGGCAACG CAACUGAACG CGGAGACGAC GGCGGGGGCC CCGGGCAGAG UUCUCUUUUC UUCUUAACGG UCUAUCACC CUGAAAACAG UUUGUCUGGA GAUAGGGUUU AAUGGCCGGA AGAGCCCGAC ACUUCUGUCG GGUCCGGUGC G CUCUCGAC GUCCCUUGAA AAUCCGCGGG AGGGAAUAAU UCUCACGCCA GGUCGUACUC AUAACCGCAG CAGGUCUCCA AG GUGAACA GCCUCUGGUU GAUAGAACAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGUAACUUCG GGAAAAGGAU UGG CUCUAA GGGUUGGGUA CGUCGGGCUU UGGGCAGACG CCCUGGGAGC AGAUCGCCAC UAGCCGGGCA ACCGGCCGGC GGCU UUCAG CAUCCGGGUG CAGAAGCCCU UAGCAGACUU CGGUCGUCCG GCGUACGUUU AACAACCAAC UUAGAACUGG UACGG ACAG GGGGAAUCUG ACUGUCUAAU UAAAACAUAG CAUUGCGAUG GCCAGAAAGU GGUGUUGACG CAAUGUGAUU UCUGCC CAG UGCUCUGAAU GUCAAAGUGA AGAAAUUCAA CCAAGCGCGG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAG CC AAAUGCCUCG UCAUCUAAUU AGUGACGCGC AUGAAUGGAU UAACGAGAUU CCCACUGUCC CUAUCUACUA UCUAGCGA A ACCACAGCCA AGGGAACGGG CUUGGCAGAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUU UGACAUUGU GAAAAGACAU AGGAGGUGUA GAAUAGGUGG GAGCUUCGGC GCCGGUGAAA UACCACUACU CCUAUUGUUU UUUUACUUAU UCAAUUAAG CGGGGCUGGA UUUUCGUCCA ACUUCUGGUU UUAAGGUCCU UCGCGGGCCG ACCCGGGUUG AAGACAUUGU C AGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC CAUAACGCAG GUGUCCUAAG GGGGGCUCAU GGAGAACAGA AA UCUCCAG UAGAACAAAA GGGUAAAAGU CCCCUUGAUU UUGAUUUUCA GUGUGAAUAC AAACCAUGAA AGUGUGGCCU AUC GAUCCU UUAGUCCCUC GAAAUUUGAG GCUAGAGGUG CCAGAAAAGU UACCACAGGG AUAACUGGCU UGUGGCGGCC AAGC GUUCA UAGCGACGUC GCUUUUUGAU CCUUCGAUGU CGGCUCUUCC UAUCAUACCG AAGCAGAAUU CGGUAAGCGU UGGAU UGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACCGUCG UGAGACAGGU UAGUUUUACC CUACUGAUGA CCUCAU CGC AAUGGUAAUU GAGCUUAGUA CGAGAGGAAC CGCUCAUUCA GAUAAUUGGU UUUUGCGGUU GUCCGACCGG GCAGUGC CG CGACGCUACC AUCUGCUGGA UAAUGGCUGA ACGCCUCUAA GUCAGAAUCC AUGCCAGAAC GCGAUGAUAC CUCCAGCA C GUUGUAGACG UAUAAGAAUA GGCUCCGGCC UCGUAUCUUA GCAGGCGAUU CCUCCGUUGG CCUUGAAGUG GCCAGCGGU AAUUCGCGUA UUGUAAUUUU AACACGCGCU GGAUCAAAUC CUUUGCAGAC GACUUAGAUG UGCGAAAGGG UCCUGUAAGC AGUAGAGUA GCCUUGUUGU UACGAUCUGC UGAGGGUAAG CCCUCCUUCG CCUAGAUUU

GENBANK: GENBANK: FJ360521.1

Macromolecule #2: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 578.821625 KDa

Sequence

String:

UACCUGGUUG AUUCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUUUAAGC AAUUAAACCG CGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUAGUUUAU UUGAUAGUAC CUUACUACAU GGAUAACCGU GGUAAUUCUA G AGCUAAUA CAUGCUAAAA ACCCCGACUU CGGAAGGGGU GUAUUUAUUA GAUUAAAAAC CAAUGCCCUU CGGGGCUAAC UG GUGAUUC AUAAUAACUU CUCGAAUCGC AUGGCCUUGC GCUGGCGAUG GUUCAUUCAA AUUUCUGCCC UAUCAACUUU CGA CGGCUG GGUCUUGGCC AGCCAUGGUG ACAACGGGUA ACGGAGGGUU AGGGCUCGAC CCCGGAGAAG GAGCCUGAGA AACG GCUAC UACAUCCAAG GAAGGCAGCA GGCGCGCAAA UUACCCAAUC CCGACACGGG GAGGUAGUGA CAAUAAAUAC UGAUA CAGG GCUCUUUUGG GUCUUGUAAU UGGAAUGAGU ACAAUUUAAA UCCCUUAACG AGGAACAAUU GGAGGGCAAG UCUGGU GCC AGCAGCCGCG GUAAUUCCAG CUCCAAUAGC GUAUAUUAAA GUUGUUGAGG UUAAAAAGCU CGUAGUUGAA CCUUGGG CU CGGCCCGUCG GUCCGCCUCA CCGCGUGCAC UGACUGGGUC GGGCCUUUUU UCCUGGAGAA CCGCAUGCCC UUCACUGG G UGUGUCGGGG AACCAGGACU UUUACCGUGA ACAAAUCAGA UCGCUCAAAG AAGGCCUAUG CUCGAAUGUA CUAGCAUGG AAUAAUAGAA UAGGACGUGU GGUUCUAUUU UGUUGGUUUC UAGGACCGCC GUAAUGAUUA AUAGGGACAG UCGGGGGCAU CAGUAUUCA AUUGUCAGAG GUGAAAUUCU UGGAUUUAUU GAAGACUAAC UACUGCGAAA GCAUUUGCCA AGGAUGUUUU C AUUAAUCA GGAACGAAAG UUAGGGGAUC GAAGACGAUC AGAUACCGUC GUAGUCUUAA CCAUAAACUA UGCCGAUUAG GG AUCGGAC GGUGUUAUUU UUUGACCCGU UCGGCACCUU ACGAUAAAUC AAAAUGUUUG GGCUCCUGGG GGAGUAUGGU CGC AAGGCU GAAACUUAAA GAAAUUGACG GAAGGGCACC ACCAGGGGUG GAGCCUGCGG CUUAAUUUGA CUCAACACGG GGAA ACUCA CCAGGUCCAG ACACGAUGAG GAUUGACAGA UUGAGAGCUC UUUCUUGAUU UCGUGGGUGG UGGUGCAUGG CCGUU CUUA GUUGGUGGAG UGAUUUGUCU GCUUAAUUGC GAUAACGAAC GAGACCUUAA CCUGCUAAAU AGCCCGUAUU GCUUUG GCA GUACGCUGGC UUCUUAGAGG GACUAUCGGC UCAAGCCGAU GGAAGUUUGA GGCAAUAACA GGUCUGUGAU GCCCUUA GA UGUUCUGGGC CGCACGCGCG CUACACUGAC ACAGCCAGCG AGUACUCCCU UGGCCGGAAG GUCCGGGUAA UCUUGUUA A ACUGUGUCGU GCUGGGGAUA GAGCAUUGCA AUUAUUGCUC UUCAACGAGG AAUCCCUAGU AAGCGCAAGU CAUCAGCUU GCGUUGAUUA CGUCCCUGCC CUUUGUACAC ACCGCCCGUC GCUACUACCG AUUGAAUGGC UCAGUGAGGC UUCCGGACUG GCCCAGGGA GGUCGGCAAC GACCACCCAG GGCCGGAAAG CUAUCCAAAC UCGGUCAUUU AGAGGAAGUA AAAGUCGUAA C AAGGUCUC CGUUGGUGAA CCAGCGGAGG GAUCAUUA

GENBANK: GENBANK: FJ360521.1

Macromolecule #3: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 38.579906 KDa

Sequence

String:

ACAUACGACC AUACCCACUG GAAAACUCGG GAUCCCGUCC GCUCUCCCAU AGAUAAGCCA GUGAGGGCCA GACUAGUAGU UGGGUCGGU GACGACCAGC GAAUCCCUGG UGUUGUAUGU U

GENBANK: GENBANK: K02469.1

Macromolecule #5: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 50.770008 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUG GCAUCGAUGA AGAACGCAGC GAAAUGCGAU AGGUAAUGUG AAUUGCAGAA UUCAGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCUC GCCAGUAUUC UGGCGAGCAU GCCUGUUCGA GCGUCAUUU

GENBANK: GENBANK: M10692.1

Macromolecule #80: tRNA

• Macromolecule: Name: tRNA / type: rna / ID: 80 ; Details: One molecule is found in the A-site, and one molecule is found in the P-site. No specific tRNAs are represented in the peptidyltransferase centers of polysomal ribosomes. Therefore, we adapted the tRNA model for a eukaryotic ICG-anticodon tRNA that decodes Arg codons (PDB ID: 6T4Q) to model the tRNA densities of both A- and P-site tRNAs.; Number of copies: 2
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 24.468551 KDa

Sequence

String:

UUCCUCGUGG CCCAAUGGUC ACGGCGUCUG GCUACGAACC AGAAGAUUCC AGGUUCAAGU CCUGGCGGGG AAGCCA

Macromolecule #82: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 82 ; Details: No specific mRNA is represented in the polysomal ribosomes. Therefore, we used Uracil to model the mRNA.; Number of copies: 1
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 5.159861 KDa

Sequence

String:

UUUUUUUUUU UUUUUUU

Macromolecule #4: 40S ribosomal protein S0

• Macromolecule: Name: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 31.529408 KDa

Sequence

String:

MAPANLPSIF NATSTDIEQL LAAQCHIGSK NLGVHMQPYL WKTRADGVNI LNVGKTWEKI VLAARIIAAI DNPADICVIS ARPYGQRAV LKFAAHTGAQ AIAGRFTPGS FTNYITRSFK EPRLIIVTDP RTDAQAIKEA SYVNIPVIAL CDADSPTEYV D VAIPTNNK GRHAIGCVWW MLAREVLRLR GTIYNRETPW DVMVDLYFYR DPEAEAEEKV EEEKLPGVEE EGVAAIESGF PA TGDWEAA PAGFPATGEW SDAAPGAAAP NWDATAPATT ADWAATEAKE SSW

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #6: 40S ribosomal protein S1

• Macromolecule: Name: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 29.12384 KDa

Sequence

String:

MAVGKNKRLS KGKKGLKKKT QDPFARKDWY GIKAPAPFNV RDVGKTLVNR TTGLKNANDA LKGRIFEVSL ADLQKDEDHS FRKVKLRVD EIQGKNCLTN FHGLDFTSDK LRSLVRKWQT LIEANITVKT TDDYLLRLFA IAFTKRRPNQ VKKTTYAASS Q IRAIRRKM TEIIQREASS CTLTQLTSKL IPEVIGREIE KATQGIYPLQ NVHIRKVKLL KQPKFDLGAL MSLHGESGSD EA GQKVERE FKETVLESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #7: 60S ribosomal protein L2

• Macromolecule: Name: 60S ribosomal protein L2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 27.410562 KDa

Sequence

String:

MGRVIRNQRK GRGSIFTANT RLNKAPAKFR SLDYAERHGY IRGVVKEIIH DPGRGAPLAR VVFNSPYKFK KQRETFIANE GMYTGQFIY AGKNAALTVG NVLPLGSVPE GTVVSNVEEK VGDRGAIGRT SGNYVTVVGH NPEEGKTRIK LPSGAKKVVS S SARGMIGI VAGGGRTDKP LLKASRAKHK FAVKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRY AAQGQKAGLI AA RRTGLLR GTQKTKE

UniProtKB: 60S ribosomal protein L2

Macromolecule #8: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 28.80733 KDa

Sequence

String:

MADRGTSGPR GGGFASRGDR GGDRGRGRGG RGRGRRGGKS EEKEWQPVTK LGRLVKAGKI KSMEEIYLHS LPIKEYQIVD FFLPKLKDE VMKIKPVQKQ TRAGQRTRFK AIVIIGDSEG HVGLGIKTSK EVATAIRAAI IIAKLSVIPV RRGYWGANLG L PHSLPVKE SGKCGSVTVR LIPAPRGTSL VASPAVKRLL QLAGIEDAYT SSSGSTKTLE NTLKATFAAV SNTYGFLTPN LW KETKLTR SPLEEFADTL REGKRYAN

UniProtKB: 40S ribosomal protein S2

Macromolecule #9: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 44.131371 KDa

Sequence

String:

MSHRKYEAPR HGSLAYLPRK RAARHRGKVK SFPKDDAKKP VHLTAAMGYK AGMTTIVRDL DRPGAKAHKK EVVEAVTIID TPPMIVVGL VGYIETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYVKKHSD NNGAAITREL ERIKKYCTVV R VLAHTQIR KTPLKQKKAH LMEIQINGGS VADKVEFGHG LFEKPVSIDS IFEKDEVIDV IAVTKGHGFT GVTARWGTKK LP RKTHKGL RKVACIGAWH PSHVQWTVAR AGQAGYHHRT SVNHKIYRIG KGDAEDSAAT EVDVTKKKIT PMGGFVRYGE INN DFVMVK GSVPGVKKRV MTLRKSMFVH TSRKALEKVE LKWIDTSSEF GHGAFQTPAE KKQFQGTLKK DLAASS

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #10: Cytoplasmic ribosomal protein 10

• Macromolecule: Name: Cytoplasmic ribosomal protein 10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 28.706771 KDa

Sequence

String:

MAVPGTQISK RRKFVADGVF YAELNEFFQR ELAEEGYSGV EVRVTPTVTD IIIRATHTQE VLGEQGRRIR ELTSLIQKRF KFPENSVSL YAAKVQNRGL SAVAQCESLR YKLLNGLAVR RACYGVLRFI MESGAKGCEV VVSGKLRAAR AKSMKFTDGF M IHSGQPAK DFIDSATRHV LLRQGVLGIK VKIMRGSDPE GKSGPQKSLP DAVTIIEPKE EQPVTQPISQ DYGAKAAQVQ AA AEAARQE EQAGEEEAAA PAAEE

UniProtKB: 40S ribosomal protein S3

Macromolecule #11: 60S ribosomal protein L4-A

• Macromolecule: Name: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 38.882977 KDa

Sequence

String:

MASRPTVTII GADGKATGTT EVLPKVFGAP IRPDIVKHVH TGMAKNKRQP YSVSEKAGHQ TSAESWGTGR AVARIPRVSG GGTHRAGQA AFGNMCRSGR MFAPTKIWRK WHVKVNQGQK RFATASALAA SAAAPLLMAR GHQVSTVPEV PLVVDSAAFS G VNKTANAL ALLKAVGAGA DVQKAKASKK LRAGKGKLRD RRHRQRRGPL VVYSAETDGK DLIKGFRNIP GVETSPVDAL NL LQLAPGG HLGRFIVWTS AAIKELDAVY ESKKGFQLPA NVVAQADLTR LINSSEIQSV LRTPKGDAKT KRSNVQKKNP LRN KQVLLR LNPYAATFAK EKLGEIKEEG KPQRVPATFK TLLQE

UniProtKB: 60S ribosomal protein L4-A

Macromolecule #12: 40S ribosomal protein S4

• Macromolecule: Name: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 29.654619 KDa

Sequence

String:

MGRGPKKHQK RLSAPSHWLL DKLSGVYAPR PSAGPHKLRE CMPLIVFVRN RLKYALNYRE TKAIMMQRLI KVDGKVRTDI TYPAGFMDV ITIEKTGENF RLIYDTKGRF TVHRIQDEEA KYKLGKVKRV QLGKGGIPFL VTHDARTIRY PDPLIKVNDT V KINLETGK IEDFVKFDTG AIAMVTGGRN MGRVGVITHR ERHDGGFNII HVKDALDNTF ATRESNVFVI GSEKPWISLP KG KGVKLSI AEERDRRRAN ALAH

UniProtKB: 40S ribosomal protein S4

Macromolecule #13: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 34.47077 KDa

Sequence

String:

MAFHKLVKNS AYYSRFQTKY KRRREGKTDY YARKRLITQA KNKYNAPKYR LVVRFTNRDI ILQIVSSEIT GDKVFASAYS HELKAYGIE HGLTNWAAAY ATGLLLARRV LKKLGLDETF KGVEEADGEY KLTEAAETDD GERRPFKAFL DVGLARTSTG A RVFGAMKG ASDGGIFIPH SENRFPGYDM ESEELDAETL KKYIFGGHVA EYMETLADDD EERYKSQFNR YIEDDLEADG LE DLYAEAH AAIREDPFKK AESEAPKKTK EEWKAESLKY KKSKLTREQR AAGVQERIAA LRSE

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #14: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 23.715213 KDa

Sequence

String:

MSEGEVDVAA VSQYEVLPKE VLAEVGSVKL FNRWSYEDVE IRDISLTDYI QIRSPVYLPH SAGRYAAKRF RKANCPIIER LTNSLMMHG RNNGKKLMAV RIVAHAFEII HLMTDQNPIQ IAVDAIVNCG PREDSTRIGS AGTVRRQAVD VSPLRRVNQA I ALLTTGAR EASFRNVKSI AECLAEELIN AAKGSSNSYA IKKKDELERV AKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #15: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 22.516178 KDa

Sequence

String:

MSSAAAPQTK TFGKATRTVP HSSEKAQKWY PAEDEAQPRK VRKTIRPWSP RSTLVPGTVL ILLAGRFRGK RVVLLKALDQ GVLLVTGPF KINGVPLRRV NARYVIATSQ KVDLAGIDEA KINEIAQPKY FTAEKSKEKA GEEAFFKQGE KPQKKEVSSS R VADQKAID KALIANIKKV EFLASYLASS FTLRKGDKPH EMKW

UniProtKB: 60S ribosomal protein L6

Macromolecule #16: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 27.395119 KDa

Sequence

String:

MKLNISYPAN GSQKLIEVED ERKLRHFIEK RMGAEVPADP LGDEWKGYIL RITGGNDKQG FPMKQGVIAP NRVRLLLSDG HSCYRARRT GERKRKSVRG CIVGSDLSVL ALAIVKQGEQ DIPGLTDVVH PKRLGPKRAT KIRRFFGLSK DDDVRKYVIR R EVQPKGEG KKPYTKAPKI QRLVTPQRLQ HKRHRLALKR RQAEKVKEEA SEYAQILAKR IAEAKVQKAD ARKRRASSMR K

UniProtKB: 40S ribosomal protein S6

Macromolecule #17: 60S ribosomal protein L7

• Macromolecule: Name: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 28.741695 KDa

Sequence

String:

MSTSVPTKND VLVPETLLKK RKSQEKARAE RQAEIEKKKA ANKEKRAVIF KRAETYVKEY RDVEREKIRL QRAAKQDGSF HIPAEAKLI FLIRIKGINK IPPKPRKILQ LLRLLQINNG VFVRVTKATA EMIKIVEPWV AYGYPNLKSV KELIYKRGYG K VNKQRVAL TDNSIIEENL GKYGIICMED LIHEIYTVGP NFKQASNFLW PFKLSNPTGG FRTRKFKHFI EGGDLGNREE HI NALIRQM N

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #18: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 22.913477 KDa

Sequence

String:

MSAPQLNKIA ANSPSRQNPS ELETAVAGAL YDLESNTADL KAALRPLQFV SAREIEVGHG KKAIVIFVPV PSLQGFHRVQ QRLTRELEK KFSDRHVLIV ASRRILPRPK RSSRSRNTLK QKRPRSRTLT AVHDAILTDL VYPVEIVGKR LRVKEDGSKT L KVILDEKE RGSVDYRLDT YSAVYRRLTG KNVLFEFPLV SAEY

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #19: 60S ribosomal protein L8

• Macromolecule: Name: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 29.427127 KDa

Sequence

String:

MPQKSGKKAA PAPFPQGKAG SKKAPKNPLI EKRPRNFGIG QAIQPKRNLS RMVKWPEYVR LQRQKKILNM RLKVPPALAQ FQQVLDKNT AAQAFKLLNK YRPETKTEKK ERLLQEATAI KEGKKKEDVS KKPYVVKYGL NHVVGLIENK KASLVLIPND V DPIELVVF LPALCRKMGI PYAIIKGKAR LGTVVHKKTA AVLALTEVRA EDKNELAKLV SAIKEGYLEK NEQARRQWGG GI MGVKSQM KIAKRKKALE NAIKV

UniProtKB: 60S ribosomal protein L8

Macromolecule #20: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 23.061273 KDa

Sequence

String:

MGISRDSRHK RSHTGAKRAY YRKKRAFEAG RQPANTRIGA KRIHTVRTRG GNHKYRALRL DSGNFAWASE GCTRKTRVIV VAYHPSNNE LVRTNTLTKS AVVQIDAAPF RQWYEAHYGQ PLGRRRQQKQ GQVVEEVKKS KSVEKKQAER FAAAGKVDPA L EKQFEAGR LYAVISSRPG QSGRCDGYIL EGEELAFYQR KLHK

UniProtKB: 40S ribosomal protein S8

Macromolecule #21: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 21.787223 KDa

Sequence

String:

MRYIHSEETL EVPENVKVSI KSRIVTVEGP RGKLVKDLSH IAVNFSVIKK GVIGLEIHHG NRKNVAALRT VRTIINNLII GVTKGFKYK MRYVYAHFPI NVNVEKNAET GNFEVEIRNF IGEKIVRRVV MQPGVDVEIS KAQKDELVLS GNSLEGVSQS A ADIQQICR VRNKDIRKFL DGIYVSEKGN IVEDQ

UniProtKB: 60S ribosomal protein L9

Macromolecule #22: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 21.852533 KDa

Sequence

String:

MAPRSYSKTA KVPRRPFEAA RLDSELKLVG EYGLRNKREV WRVLLTLSKI RRAARQLLTL DEKDPKRLFE GNALIRRLVR VGVLDESRM KLDYVLALKA EDFLERRLQT CVYKLGLAKS IHHARVLIRQ RHIRVGKQIV NVPSFVVRLD SQKHIDFALT S PFGGGRPG RVRRKKAKAA EGGEAAEEED EE

UniProtKB: 40S ribosomal protein S9

Macromolecule #23: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 25.373594 KDa

Sequence

String:

MARRPARCYR YCKNKPYPKS RFNRGVPDPK IRIFDLGRKR ATVDDFPLCI HMVSNEYEQL SSEALEAARI CANKYLVKTA GKEGFHLRV RVHPYHVVRI NKMLSCAGAD RLQTGMRGAW GKPNGTVARV NIGQIIMSVR TRDSNKAFAL EALRRSQYKF P GRQKIIIS KNWGFTPLRR EEYLEKKAAG KLLIDGAYVQ FLGNRGSLER NMKRFPDAFA TEA

UniProtKB: 60S ribosomal protein L10

Macromolecule #24: Probable 40s ribosomal protein s10-b

• Macromolecule: Name: Probable 40s ribosomal protein s10-b / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 18.463754 KDa

Sequence

String:

MLIPKADRKA IHEYLFREGV MVAEKNYEST HETGIRNLYV IKACQSLTSR GYVKTQFSWQ YYYYTLTPEG LDYLREWLHL PAEIVPATH IKSQRSHAPP RGMLGGEERR ERPFGGRGRG DREGGYRRRE AGEGKEGGAP GEFAPQFRGG FGRGRAGRGE A PPS

UniProtKB: Probable 40s ribosomal protein s10-b

Macromolecule #25: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 20.123266 KDa

Sequence

String:

MSTEQKAQNP MRELRIQKLV LNICVGESGD RLTRAAKVLE QLSGQTPVYS KARYTVRTFG IRRNEKISVH VTVRGPKAEE ILERGLKVK EYELRKRNFS ETGNFGFGIS EHIDLGIKYD PGIGIYGMDF YCCMTRPGER VSRRRRAKSR VGATHRITRD D TVKWFKSR FDAIVR

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #26: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 18.468691 KDa

Sequence

String:

MATELTVQSE RAFQKQPHIF LNSKTKVKSA RPGKGGRRWY KDVGLGFRTP AAAIEGQYID KKCPFTGQVS IRGRILSGTV VSTKMHRTI IIRREYLHFI PKYNRYEKRH KNLAAHVSPA FRVEEGDKVT VGQCRPLSKT VRFNVLRVLP RTGKSVKKFA K F

UniProtKB: 40S ribosomal protein S11

Macromolecule #27: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 23.929869 KDa

Sequence

String:

MAIKHNQQIP NNHFRKDWQR RVRCHFDQAG KKASRRVARQ AKAAALAPRP VDKLRPIVRC PTIKYNRRTR LGRGFSLAEL KAAGIPKLV APTIGISVDP RRANLSEESL AANVERLKAY QARLVVFPRK SNKPKKADTP KDQQTGEFVK SVEAVFGVER P IASGFKEI PKSELPSNIE GGAFRALRKA RSDAKLIGVR EKRAKDKAAA EAEKSK

UniProtKB: 60S ribosomal protein L13

Macromolecule #28: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 16.250645 KDa

Sequence

String:

MSDVEENTVP ASEEVEVSAD AAPKGQMSVL DALKGVLKIS LMHDGLARGL REASKALDRR EAHMCVLNES CEEEAYKKLV IALCSEHKI PLIKVPDGKQ LGEWAGLCVL DREGNARKVV NCSCVVVKNW GEMSSEREIL LNYFQTEQ

UniProtKB: 40S ribosomal protein S12

Macromolecule #29: Ribosomal protein L14

• Macromolecule: Name: Ribosomal protein L14 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 15.885671 KDa

Sequence

String:

MADIQIGSSA WRLVEVGRVL KLEGGSLATI VEIVDHKRVL VDGPSSDPKL AAPRGVVSLS RTLLTPLVVE KLPRGARTGA VKKAWEAAG IDAKWKESNW AKKQLQQERR KALTDFDRFK VMRLKKQRRF EERKALAKIK ASA

UniProtKB: Ribosomal protein L14

Macromolecule #30: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 16.90491 KDa

Sequence

String:

MGRMHSKGKG ISASAIPYSR NPPSWLKTTP EQVVDQICKL AKKGATPSQI GVILRDSHGI AQVKVVTGNK ILRILKSNGL APDLPEDLY MLIKKAVAVR KHLERNRKDK DSKFRLILIE SRIHRLARYY KTVGVLPPTW KYESSTASTI VS

UniProtKB: 40S ribosomal protein S13

Macromolecule #31: 60S ribosomal protein L15

• Macromolecule: Name: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 24.250232 KDa

Sequence

String:

MGALKYLEEL QKKKQSDVVR FLLRVRCWEL RQLNVIHRAS RPSRPDKARR LGYKAKQGYV IYRARVRRGG RKKPVPKGAT YGKPTNQGV NQLKYQRSLK STAEERVGRR CANLRVLNSY WINQDSTYKY FEVILVDPQH KAIRRDPRIN WIVNPVHKHR E SRGLTSTG KRSRGLNKGH RYNKTRAGRR KTWKRHNTLS LWRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #32: 40S ribosomal protein S14 (uS11)

• Macromolecule: Name: 40S ribosomal protein S14 (uS11) / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 16.025438 KDa

Sequence

String:

MPPKKAARPA QENISLGPQI REGELVFGVA RIFASFNDTF VHVTDLSGRE TICRVTGGMK VKADRDESSP YAAMLAAQDV AARCKELGI TALHIKIRAT GGNGTKTPGP GAQSALRALA RSGMKIGRIE DVTPTPSDST RRKGGRRGRR L

UniProtKB: Uncharacterized protein

Macromolecule #33: 60S ribosomal protein L16

• Macromolecule: Name: 60S ribosomal protein L16 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 26.184609 KDa

Sequence

String:

MSQHQAHGQF SAHTKKSQTV NHRESSPAKM SSFESVVVID GKGHLLGRLA SIVAKQLLNG QKIVVVRCEA LNISGEFFRA KLKYHSYLR KMTRYNPTRG GPFHFRAPSR IFYKAVRGMI PHKTARGAAA LERLKVFEGV PPPYDKKKKM VVPQALRVLR L QPGRKFCT VGRLSSEVGW KYEDVVSRLE ERRKAKGAAY YERKKVAARQ LSEAKKSAKV NDKTAEALKE FGY

UniProtKB: 60S ribosomal protein L16

Macromolecule #34: Ribosomal protein S12

• Macromolecule: Name: Ribosomal protein S12 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 17.419408 KDa

Sequence

String:

MADEYNAEEA AELKKKRTFR KFSYRGIDLD ALLDLGSDEL RDVVHARARR RINRGLKRKP MGLIKKLRKA KQEAKPNEKP DLVKTHLRD MIVVPEMIGS VIGIYSGKEF NQVEIKPEMV GHYLAEFSIS YKPVKHGRPG IGATHSSRFI PLK

UniProtKB: Ribosomal protein S12

Macromolecule #35: 60S ribosomal protein L17

• Macromolecule: Name: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 20.802846 KDa

Sequence

String:

MVRYAATEIA PAKSARSRGS YLRVSFKNTR ETAQAINGWK LQRAQKFLEN VLEKKEAVPM RRYAGGTGRA AQGKQFGVSR ARWPAKSAE FLLGLLKNAE ANADAKGLDT GNLVVKHIQV NQAPKQRRRT YRAHGRINPY MSNPCHIELI LTEADEVVQK S EAVVREEA HLSSRQRGAR VRRAITAA

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #36: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 15.752556 KDa

Sequence

String:

MATQAVQVFG KKKNATAVAR CVQGKGLIKV NGVPLKLYAP EILRAKLYEP ILLLGTDKFA EVDIRLKVSG GGHVSQVYAV RQAIAKAIV AYYAKYVDEH SKNTLKTALI QFDRTLLVAD PRRCEPKKFG GKGARSRFQK SYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #37: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 20.6514 KDa

Sequence

String:

MGIDLRKHHE RSTHRKAPKS DNVYLKLLVK LYRFLARRTD SAFNKVVLRR LFMSRINRPP MSISRIVANL KGNEKKTVVV VGTVTDDNR LLTVPKVSVA ALRFTATARA RIEAAGGQAI TLDQLALEKP TGANTLLLRG PKNAREAVKH FGFGPHKHKK P YVASKGRK FEKARGRRRS KGFKV

UniProtKB: 60S ribosomal protein L18

Macromolecule #38: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 16.974506 KDa

Sequence

String:

MGRVRTKTVK KSAKVIIERY YPKLTLDFET NKRICDEIAI IASKRLRNKI AGYTTHLMKR IQRGPVRGIS FKLQEEERER KDQYVPEVS ALDFTQNSES GQLDVDTETK DLLKHLGFDS IPVNVIPVTQ AQPVERGRRF GDRPRRD

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #39: Ribosomal protein L19

• Macromolecule: Name: Ribosomal protein L19 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 22.407271 KDa

Sequence

String:

MVNLRTQKRL ASSVLGVGQR KIWLDPNEVS EISNANSRQT IRKLVADGLI IKKPVTMHSR SRARELNLAR RIGRHRGFGK RKGTADARM PEQVLWMRRQ RVLRRLLVKY RASGKIDKHL YHELYHLAKG NTFKHKRALV EHIHRAKAEK AREKAIKEEM D AKRARTKA ARERKLERQA AKRNALAGEE EESK

UniProtKB: Ribosomal protein L19

Macromolecule #40: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 17.806611 KDa

Sequence

String:

MSLVSGEKSN FNHILRLLNT NVDGKQKVVY SLTKIKGVGR RYSNLVCKKA DVDLSKRAGE LTSEELERIV TIMQNPTAYK IPAWFLNRQ RDIVDGKDSQ ILANGVDSKL RDDLERLKKI RAHRGLRHYW GLRVRGQHTK TTGRRGRTVG VSKKKGN

UniProtKB: 40S ribosomal protein S18

Macromolecule #41: 60S ribosomal protein L20

• Macromolecule: Name: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 20.385027 KDa

Sequence

String:

MGRLQEYQVI GRHLPTESNP TPALYRMRIF APNAVVAKSR FWYFLRGLRK VKKATGEIVT CNVITEKHPL KVKNFGVWIR YDSRSGTHN MYKEYRELSR TAAVEALYSD MAARHRARFR SIHILKVVEL EKTEDIKRPY LKQLVTKKLS FPLPHRVPKI S NKKIFSAK RPATFA

UniProtKB: 60S ribosomal protein L20

Macromolecule #42: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 16.700889 KDa

Sequence

String:

MPGGVTVRDV EPHKFVNAYA AFLKRQGKLP VPGWVDTVKT GPAKEMPPQD IDWFYVRAAS VARHVYLRKT VGVGRLRRVH GTAKNRGSR PSHHVEASGS VDRKVLQALE KIGVLEHDEE KGGRRITQQG QRDLDRIAQT VIEADEEDDE

UniProtKB: 40S ribosomal protein S19

Macromolecule #43: Ribosomal protein Srp1

• Macromolecule: Name: Ribosomal protein Srp1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 18.253395 KDa

Sequence

String:

MGHAAGLRAG TRYAFSRNFR EKGMIRLSTY LKQYKVGDIV DIKVNGAVQK GMAHKVYHGK TGVIYNVTKS AVGIIIYKKV KHRYIEKRI NVRIEHIQPS RSRDDFLRRV KQNAELKKQA KAEGKPVQLK RQALLPREAR TISVVDNKPE TVAPVAYETT I

UniProtKB: Ribosomal protein Srp1

Macromolecule #44: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 13.250597 KDa

Sequence

String:

MSSYNKDQKD FGEAPKVHRV RITLTSRKVQ ALEKVCSELV ERAKTKDLRV KGPVRLPTKI LKITTRKSPC GEGSKTWDLF EMRIHKRLI DLTAPTEIVK QIIVNIEAGV EVEVTIAA

UniProtKB: 40S ribosomal protein S20

Macromolecule #45: 60S ribosomal protein L22

• Macromolecule: Name: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 14.234482 KDa

Sequence

String:

MAPITKKSAK GKGPKVTKKF VINASQPASD KIFDVSAFEK FLTEKIKVEG RVGNLGDAIK ISQVGDGKIE IVAHNELSGR YLKYLTKKF LKKMQLRDWL RVVSTSRGVY ELKFFNVVND EAEEDEE

UniProtKB: 60S ribosomal protein L22

Macromolecule #46: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 9.674954 KDa

Sequence

String:

MENDRGEIVD LYVPRKCSAT GRIIKSKDHG SCQITIAKVD ENGRAIQGEN IIYALSGFVR AMGESDDSLN RLAQRDGLLK AVWNPQR

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #47: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 14.722408 KDa

Sequence

String:

MAKQSRGAPG GKLKMTLGLP VGAIMNCADN SGARNLYIIS VKGIGARLNR LPAGGVGDMV MATVKKGKPE LRKKVHPAVI VRQSKPWKR FDGVFLYFED NAGVIVNPKG EMKGSAITGP VGKEAAELWP RIASNSGVVM

UniProtKB: 60S ribosomal protein L23

Macromolecule #48: 40S ribosomal protein S22

• Macromolecule: Name: 40S ribosomal protein S22 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 14.846377 KDa

Sequence

String:

MVRTSVLHDA LNSINNAEKA GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EEVDDHRSGK IVVQLNGRMN KCGVISPRYN VRLAELEKW VVKLLPARQF GYVILTTSAG IMDHEEARRK HVSGKIIGFF Y

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #49: 60S ribosomal protein L24

• Macromolecule: Name: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 17.656621 KDa

Sequence

String:

MRTYDDTFSG QRIYPGKGKL YVRGDSKIFR FQNGKSESLF LQRKNPRRIA WTVLYRRQHK KGISEEVAKK RSRRTVKAQR AIVGASLEV IKERRSMRPE ARSAARLAAI KESKAKKQET QAAKKAEKAK NAANPKARVT SKQGAKGAPV KVAAKSR

UniProtKB: Large ribosomal subunit protein eL24

Macromolecule #50: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 15.944671 KDa

Sequence

String:

MSGGKPRGLN AARKLRNNRR EQRWSDLHYK KRALGTAFKS SPFGGSSHAK GIVLEKVGVE AKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VGLLALWKEK KEKPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #51: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 17.15816 KDa

Sequence

String:

MAPKDTKKGG ASKAGKGAQA KKAAQAALKG VHSHKKTKVR YSTTFHRPKT LQLSRAPKYP RKSIPHEPRL DEHKVIVHPL NTEGALKKI EEQNTLVFIV DVKANKAQIK QALKKLYDID TVKINTLIRP DGSKKAFARL TADVDALDIA ATKLGLV

UniProtKB: 60S ribosomal protein L25

Macromolecule #52: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 15.5983 KDa

Sequence

String:

MADSDSPVTL RTRKFIRNPL LGRKQMVVDI LHPNRPNISK DELREKLATL YKATKDQVNV FGLRTQFGGG KTTGFALVYD SPEAMKKFE PHYRLVRVGL ASKIEKASRQ QRKQRKNRQK TLRGTAKVKG AKPKKEK

UniProtKB: 40S ribosomal protein S24

Macromolecule #53: Ribosomal protein L26

• Macromolecule: Name: Ribosomal protein L26 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 15.366948 KDa

Sequence

String:

MVKVNNSVSS SRRKARKAHF SAPSSVRRNI MSAPLSKELR EKYNVRSIPI RKDDEVTIVR GSNKDKEGKV TSVYRLKYVI HVERVTRDK ATGASVPVGI HPSNVVITKL KLDKDREAIL ERIKRGRELA KPTKVSA

UniProtKB: Ribosomal protein L26

Macromolecule #54: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 10.822743 KDa

Sequence

String:

MAPAASGAKK QKKKWSKGKV KDKAQHAVIL DKSTSDKLYK DVQSYRLVTV ATLVDRLKIN GSLARRCLKD LEEKGQIKQV VGHSKMKIY TRAIGADE

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #55: 60S ribosomal protein L27 (eL27)

• Macromolecule: Name: 60S ribosomal protein L27 (eL27) / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 15.75651 KDa

Sequence

String:

MKFLKTSRVC LVTRGRYAGK KVVIIQPVDN GSKTHPYGHA IVAGIERYPS KITRRMSKTR QEKRSKVKPF IKVINYNHLM PTRYTLELE GLKGSVSAET FKEVSQREDA KKTVKKVLEE RYTSGKNRWF FTPLRF

UniProtKB: 60S ribosomal protein L27

Macromolecule #56: 40S ribosomal protein S26E

• Macromolecule: Name: 40S ribosomal protein S26E / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 13.580064 KDa

Sequence

String:

MVKKRKNNGR NKKGRGHVKP IRCSNCSRCT PKDKAIKRFT IRNMVESAAI RDISDASVFA EYTVPKMYLK LQYCVSCAIH GKIVRVRSR EGRRNRAPPP RVRYNKDGKK VTPTQGAKTA

UniProtKB: Small ribosomal subunit protein eS26

Macromolecule #57: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 16.636375 KDa

Sequence

String:

MPTRFSKTRK HRGHVSAGKG RVGKHRKHPG GRGMAGGQHH HRTNLDKYHP GYFGKVGMRH FHLLRNHQWA PILNIEKLWT LVPAEAREK YVSGAATETA PVIDLLSHGY AKLLGKGRLP QVPIVVRARY VSAEAERKIK EAGGVIELVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #58: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 8.910573 KDa

Sequence

String:

MVLAVDLLNP SPASEARKHK LKTLVPAPRS FFMDVKCPGC FTITTVFSHA QTVVICQGCT TVLCQPTGGK ARLTEGCSFR RK

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #59: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 7.488608 KDa

Sequence

String:

MAKSKNSSQH NQSRKAHRNG IKKPKTSRYP SLKGTDPKFR RNHRHALHGT AKALKEFKEG KRETA

UniProtKB: 60S ribosomal protein L29

Macromolecule #60: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 7.735974 KDa

Sequence

String:

MDSSKAPVKL VKVTRVLGRT GSRGGVTQVR VEFMDDQTRS IIRNVKGPVR EDDILVLLES EREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #61: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 11.720708 KDa

Sequence

String:

MAPKKSKSDA QSIGAKLALV IKSGKVVLGY RSTLKALRSG KAKLILISAN TPPLRKSELE YYSMMSKTAV HHYTGTNIEL GTACGKLFR CSTMAILDAG DSDILADQQQ

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #62: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 6.515507 KDa

Sequence

String:

MSHESVWNSR PRTYGKGSRS CRVCTHSAGL IRKYGLNICR QCFREKANDI GFTKHR

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #63: 60s ribosomal protein

• Macromolecule: Name: 60s ribosomal protein / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 14.097444 KDa

Sequence

String:

MSSKKPTGKT QRSAIADVVA REYTIHMHKR MHGVTFKKRA PRAIKEIKEF AYKAMGTTDV RIDPQLNKKV WEQGVKGVAY RLRVRISRR RNDEEGAKEK LYSYVQAVNV KNPKGLHTVV VEE

UniProtKB: 60s ribosomal protein

Macromolecule #64: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 6.912104 KDa

Sequence

String:

MGKVHGSLAR AGKVKSQTPK VEPQEKPKTP KGRAHKREIY TRRFVNVVTG PGGKRKMNAN PSA

UniProtKB: 40S ribosomal protein S30

Macromolecule #65: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 15.001829 KDa

Sequence

String:

MVAAKKHVPI VKKRSKGFMR HQSDRFMRVD SAWRKPKGID NRVRRRFRGT LAMPSIGYGS NKKTRHMMPS GHKAFLVSNV KDVELLLMH NKTYAAEIAH NVSSRKRIEI IARAKQLSVK VTNAKAKVTT EV

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #66: Ubiquitin-40S ribosomal protein S27a

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 17.775771 KDa

Sequence

String:

MQIFVKTLTG KTITLEVESS DTIDNVKQKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIKHKRKKTK LAVLKYYKVD SDGKIERLRR ECPNETCGAG VFMAAMQDRQ YCGRCHLTYV FEKSS

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #67: 60S ribosomal protein L33

• Macromolecule: Name: 60S ribosomal protein L33 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 12.203169 KDa

Sequence

String:

MPTEAGHRLY VKGRHLSYQR GRHTTHPKTS LIKIEGVDDT AAANFYLGKR VAYVYRGQKE VRGTKIRVIW GKVTRPHGNS GVVRAKFAA PLPSRSFGAS VRIMLYPSSI

UniProtKB: 60S ribosomal protein L33

Macromolecule #68: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 35.173578 KDa

Sequence

String:

MAEQLILKGT LEGHNGWVTS LATSLENPNM LLSGSRDKSL IIWNLTRDET SYGYPKRRLH GHSHIVSDCV ISSDGAYALS ASWDKTLRL WELSTGTTTR RFVGHTNDVL SVSFSADNRQ IVSGSRDRTI KLWNTLGDCK FTITEKGHTE WVSCVRFSPN P QNPVIVSS GWDKLVKVWE LSSCKLQTDH IGHTGYINAV TISPDGSLCA SGGKDGTTML WDLNESKHLY SLNANDEIHA LV FSPNRYW LCAATSSSII IFDLEKKSKV DELKPEFQNI GKKSREPECV SLAWSADGQT LFAGYTDNII RAWGVMSRA

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #69: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 13.225604 KDa

Sequence

String:

MASSRVTYRR RNPYNTTSNK TRIVKTPGGQ LRVLHIKKRG TAPKCGDCGI KLPGVPALRP REYAQLSKPK KTVQRAYGGS RCGNCVRDR VVRAFLIEEQ KIVKKVLKEQ SQAEKSKK

UniProtKB: 60S ribosomal protein L34

Macromolecule #70: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 14.445221 KDa

Sequence

String:

MSSGKVKAGA LWSKNKDELT KQLGELKTEL GQLRIQKIVS SGTKLNKIHD LRKSIARVLT IINAKQRAQL RLFYKNKKYL PLDLRAKQT RAIRRRLSKE DASRVLEKTK KRTTHFPLRK FAVKAA

UniProtKB: 60S ribosomal protein L35

Macromolecule #71: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 11.587708 KDa

Sequence

String:

MAAESKTGLA VGLKKGHKTT ARVSKPRVSR TKGHLSKRTA FVRSIVQEVA GLAPYERRVI ELLRNSKDKR ARKLAKKRLG TFGRAKAKV EDLQRVIAEA RRAGH

UniProtKB: 60S ribosomal protein L36

Macromolecule #72: Ribosomal protein L37

• Macromolecule: Name: Ribosomal protein L37 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 10.261002 KDa

Sequence

String:

MTKGTSSFGK RHTKTHGLCR RCGRRSLHNQ KKVCASCGYP AAKTRKYNWS EKAKRRKTTG TGRLRYLSTV SRKFKNGFQT GVPKGSKGP VAA

UniProtKB: Ribosomal protein L37

Macromolecule #73: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 9.169931 KDa

Sequence

String:

MPQEIGDIKK FIEICRRKDA SAARIKKNKA TQQIKFKVRC QKYLYTLVLK DSDKAEKLKA SLPPSLTIAD VAKRNKKQTA

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #74: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 6.283508 KDa

Sequence

String:

MPSHKTFRVK QKLAKAQKQN RPIPQWIRLR TGNTIRYNAK RRHWRKTRLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #75: 60S ribosomal protein L40

• Macromolecule: Name: 60S ribosomal protein L40 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 6.092504 KDa

Sequence

String:

MIEPSLKALA SKFNCDKMIC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

UniProtKB: 60S ribosomal protein L40

Macromolecule #76: hypothetical protein NCU16635 (eL41)

• Macromolecule: Name: hypothetical protein NCU16635 (eL41) / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 3.384335 KDa

Sequence

String:

MRAKWRKKRV RRLKRKRRKM RARSK

UniProtKB: 60S ribosomal protein L41

Macromolecule #77: 60S ribosomal protein L44

• Macromolecule: Name: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 11.945273 KDa

Sequence

String:

MVNVPKTRKT YCAGRSCGKH TLHKVTQYKA GKASAFAQGK RRYDRKQSGY GGQTKPVFHK KAKTTKKVVL RLECSVCKQK KQLPLKRCK HFELGGDKKT KGAALVF

UniProtKB: 60S ribosomal protein L44

Macromolecule #78: 60S ribosomal protein L43

• Macromolecule: Name: 60S ribosomal protein L43 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 10.154967 KDa

Sequence

String:

MSKRTKKVGI SGKYGTRYGA SLRKLVKKQE VSQHARYTCT FCGKNTVRRS SVGIWSCKAC KKTLAGGAYT VSTPAAAAMR STLRRLREI TEV

UniProtKB: 60S ribosomal protein L43

Macromolecule #79: Ribosomal_L28e domain-containing protein

• Macromolecule: Name: Ribosomal_L28e domain-containing protein / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 16.011481 KDa

Sequence

String:

MSLSNVSADL IWEITRSHNS FLVKRKSGGG VQFSRDPLNL TNKHSRKYAG FVNDKAVGVV PGEKGGVVVT TKKAGSINKP AARYTVAFG ANKTARKTYK AIARQVAGYR SDLLKAAVAR ASAIRRSQRA VKPEPAQKLR GNAAKKAAAS A

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #81: Nascent peptide

• Macromolecule: Name: Nascent peptide / type: protein_or_peptide / ID: 81 ; Details: No specific nascent peptide is represented in the polysomal ribosomes. Therefore, we used UNK (unknown residue) to represent each residue of the nascent peptide.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Neurospora crassa (fungus)
• Molecular weight: Theoretical: 358.434 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)

Macromolecule #83: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethy...

• Macromolecule: Name: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione; type: ligand / ID: 83 / Number of copies: 1 / Formula: 3HE
• Molecular weight: Theoretical: 281.347 Da

Chemical component information

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione

Macromolecule #84: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 84 / Number of copies: 1 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Macromolecule #85: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 85 / Number of copies: 260 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 32.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

4u3u

Details: 4U3U and 6T4Q

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 196154
• Initial angle assignment: Type: PROJECTION MATCHING
• Final angle assignment: Type: MAXIMUM LIKELIHOOD