+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-23542 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of full-length GluK1 with L-Glu | |||||||||
Map data | GluK1 with L-Glu (full-length | |||||||||
Sample |
| |||||||||
Function / homology | Function and homology information : / negative regulation of synaptic transmission, GABAergic / L-glutamate transmembrane transporter activity / positive regulation of gamma-aminobutyric acid secretion / Activation of Na-permeable kainate receptors / kainate selective glutamate receptor complex / Activation of Ca-permeable Kainate Receptor / negative regulation of synaptic transmission, glutamatergic / regulation of short-term neuronal synaptic plasticity / inhibitory postsynaptic potential ...: / negative regulation of synaptic transmission, GABAergic / L-glutamate transmembrane transporter activity / positive regulation of gamma-aminobutyric acid secretion / Activation of Na-permeable kainate receptors / kainate selective glutamate receptor complex / Activation of Ca-permeable Kainate Receptor / negative regulation of synaptic transmission, glutamatergic / regulation of short-term neuronal synaptic plasticity / inhibitory postsynaptic potential / glutamate receptor activity / glutamate binding / adult behavior / behavioral response to pain / membrane depolarization / kainate selective glutamate receptor activity / ligand-gated monoatomic ion channel activity / ionotropic glutamate receptor complex / extracellularly glutamate-gated ion channel activity / glutamate-gated receptor activity / excitatory postsynaptic potential / regulation of membrane potential / ionotropic glutamate receptor signaling pathway / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / synaptic transmission, glutamatergic / positive regulation of synaptic transmission, GABAergic / regulation of synaptic plasticity / modulation of chemical synaptic transmission / terminal bouton / signaling receptor activity / presynaptic membrane / nervous system development / chemical synaptic transmission / postsynaptic membrane / postsynaptic density / receptor complex / neuronal cell body / dendrite / synapse / glutamatergic synapse / membrane / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Rattus norvegicus (Norway rat) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.6 Å | |||||||||
Authors | Meyerson JR / Selvakumar P | |||||||||
Citation | Journal: Cell Rep / Year: 2021 Title: Structural and compositional diversity in the kainate receptor family. Authors: Purushotham Selvakumar / Joon Lee / Nandish Khanra / Changhao He / Hermany Munguba / Lisa Kiese / Johannes Broichhagen / Andreas Reiner / Joshua Levitz / Joel R Meyerson / Abstract: The kainate receptors (KARs) are members of the ionotropic glutamate receptor family and assemble into tetramers from a pool of five subunit types (GluK1-5). Each subunit confers distinct functional ...The kainate receptors (KARs) are members of the ionotropic glutamate receptor family and assemble into tetramers from a pool of five subunit types (GluK1-5). Each subunit confers distinct functional properties to a receptor, but the compositional and stoichiometric diversity of KAR tetramers is not well understood. To address this, we first solve the structure of the GluK1 homomer, which enables a systematic assessment of structural compatibility among KAR subunits. Next, we analyze single-cell RNA sequencing data, which reveal extreme diversity in the combinations of two or more KAR subunits co-expressed within the same cell. We then investigate the composition of individual receptor complexes using single-molecule fluorescence techniques and find that di-heteromers assembled from GluK1, GluK2, or GluK3 can form with all possible stoichiometries, while GluK1/K5, GluK2/K5, and GluK3/K5 can form 3:1 or 2:2 complexes. Finally, using three-color single-molecule imaging, we discover that KARs can form tri- and tetra-heteromers. | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_23542.map.gz | 117.9 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-23542-v30.xml emd-23542.xml | 13.8 KB 13.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_23542_fsc.xml | 11.4 KB | Display | FSC data file |
Images | emd_23542.png | 54 KB | ||
Others | emd_23542_additional_1.map.gz emd_23542_additional_2.map.gz | 2.3 MB 98.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-23542 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-23542 | HTTPS FTP |
-Related structure data
Related structure data | 7lvtMC M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_23542.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | GluK1 with L-Glu (full-length | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.096 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Additional map: GluK1 with L-Glu (ATD)
File | emd_23542_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | GluK1 with L-Glu (ATD) | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Additional map: GluK1 with L-Glu (LBD-TMD)
File | emd_23542_additional_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | GluK1 with L-Glu (LBD-TMD) | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
-Entire : GluK1 tetrameric complex
Entire | Name: GluK1 tetrameric complex |
---|---|
Components |
|
-Supramolecule #1: GluK1 tetrameric complex
Supramolecule | Name: GluK1 tetrameric complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
---|---|
Source (natural) | Organism: Rattus norvegicus (Norway rat) |
Recombinant expression | Organism: Homo sapiens (human) |
-Macromolecule #1: Isoform Glur5-2 of Glutamate receptor ionotropic, kainate 1
Macromolecule | Name: Isoform Glur5-2 of Glutamate receptor ionotropic, kainate 1 type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Rattus norvegicus (Norway rat) |
Molecular weight | Theoretical: 102.562078 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MKIISPVLSN LVFSRSIKVL LCLLWIGYSQ GTTHVLRIGG IFETVENEPV NVEELAFKFA VTSINRNRTL MPNTTLTYDI QRINLFDSF EASRRACDQL ALGVAALFGP SHSSSVSAVQ SICNALEVPH IQTRWKHPSV DSRDLFYINL YPDYAAISRA V LDLVLYYN ...String: MKIISPVLSN LVFSRSIKVL LCLLWIGYSQ GTTHVLRIGG IFETVENEPV NVEELAFKFA VTSINRNRTL MPNTTLTYDI QRINLFDSF EASRRACDQL ALGVAALFGP SHSSSVSAVQ SICNALEVPH IQTRWKHPSV DSRDLFYINL YPDYAAISRA V LDLVLYYN WKTVTVVYED STGLIRLQEL IKAPSRYNIK IKIRQLPPAN KDAKPLLKEM KKSKEFYVIF DCSHETAAEI LK QILFMGM MTEYYHYFFT TLDLFALDLE LYRYSGVNMT GFRLLNIDNP HVSSIIEKWS MERLQAPPRP ETGLLDGMMT TEA ALMYDA VYMVAIASHR ASQLTVSSLQ CHRHKPWRLG PRFMNLIKEA RWDGLTGRIT FNKTDGLRKD FDLDIISLKE EGTE KIGIW NSNSGLNMTD GNRDRSNNIT DSLANRTLIV TTILEEPYVM YRKSDKPLYG NDRFEGYCLD LLKELSNILG FLYDV KLVP DGKYGAQNDK GEWNGMVKEL IDHRADLAVA PLTITYVREK VIDFSKPFMT LGISILYRKP NGTNPGVFSF LNPLSP DIW MYVLLACLGV SCVLFVIARF TPYEWYNPHP CNPDSDVVEN NFTLLNSFWF GVGALMQQGS ELMPKALSTR IVGGIWW FF TLIIISSYTA NLAAFLTVER MESPIDSADD LAKQTKIEYG AVRDGSTMTF FKKSKISTYE KMWAFMSSRQ QSALVKNS D EGIQRVLTTD YALLMESTSI EYVTQRNCNL TQIGGLIDSK GYGVGTPIGS PYRDKITIAI LQLQEEGKLH MMKEKWWRG NGCPEEDSKE ASALGVENIG GIFIVLAAGL VLSVFVAIGE FLYKSRKNND VEQCLSFNAI MEELGISLKN QKKLKKKSRT KGKSSFTSI LTCHQRRTQR KETVA |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
---|---|
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TALOS ARCTICA |
---|---|
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 52.0 e/Å2 |
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |