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- EMDB-13444: Stacked compact Dunaliella PSII -

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Basic information

Entry
Database: EMDB / ID: EMD-13444
TitleStacked compact Dunaliella PSII
Map data
Sample
  • Complex: Dunaliella salina Photosystem II complex
    • Protein or peptide: x 25 types
  • Ligand: x 30 types
Keywordsgreen algae / photosystem II / thylakoid / oxygen evolving complex / cryo-EM / stacking / PHOTOSYNTHESIS
Function / homology
Function and homology information


plastoglobule / photosynthesis, light harvesting in photosystem I / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / chloroplast envelope / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / photosynthetic electron transport chain ...plastoglobule / photosynthesis, light harvesting in photosystem I / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / chloroplast envelope / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / photosynthetic electron transport chain / response to herbicide / photosystem II / chloroplast thylakoid membrane / phosphate ion binding / photosynthetic electron transport in photosystem II / chlorophyll binding / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / response to light stimulus / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding
Similarity search - Function
Photosystem II reaction centre protein Ycf12 / Photosystem II complex subunit Ycf12 / Photosystem II PsbZ, reaction centre / Photosystem II PsbZ superfamily / YCF9 / Photosystem II PsbT / Photosystem II PsbL / Photosystem II PsbL superfamily / Photosystem II PsbT superfamily / Photosystem II reaction centre T protein ...Photosystem II reaction centre protein Ycf12 / Photosystem II complex subunit Ycf12 / Photosystem II PsbZ, reaction centre / Photosystem II PsbZ superfamily / YCF9 / Photosystem II PsbT / Photosystem II PsbL / Photosystem II PsbL superfamily / Photosystem II PsbT superfamily / Photosystem II reaction centre T protein / PsbL protein / Photosystem II CP43 reaction centre protein / Photosystem II CP43 reaction centre protein superfamily / Photosystem II PsbK / Photosystem II PsbK superfamily / Photosystem II 4 kDa reaction centre component / Photosystem II CP47 reaction centre protein / Photosystem II PsbI / Photosystem II PsbI superfamily / Photosystem II reaction centre I protein (PSII 4.8 kDa protein) / Chlorophyll A-B binding protein, plant and chromista / Photosystem II reaction centre protein H / Photosystem II D2 protein / Photosystem II cytochrome b559, conserved site / Photosystem II cytochrome b559, alpha subunit / Photosystem II cytochrome b559, beta subunit / Photosystem II cytochrome b559, N-terminal / Photosystem II cytochrome b559, alpha subunit, lumenal region / Photosystem II reaction centre protein H superfamily / Photosystem II cytochrome b559, alpha subunit superfamily / Cytochrome b559, alpha (gene psbE) and beta (gene psbF)subunits / Lumenal portion of Cytochrome b559, alpha (gene psbE) subunit / Photosystem II 10 kDa phosphoprotein / Cytochrome b559 subunits heme-binding site signature. / Chlorophyll A-B binding protein / Chlorophyll A-B binding protein / Photosystem antenna protein-like / Photosystem antenna protein-like superfamily / Photosystem II protein / Photosystem II protein D1 / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature.
Similarity search - Domain/homology
Photosystem II D2 protein / Photosystem II CP47 reaction center protein / Photosystem II reaction center protein H / Cytochrome b559 subunit beta / Chlorophyll a-b binding protein, chloroplastic / Photosystem II reaction center protein K / Photosystem II reaction center protein I / Photosystem II CP43 reaction center protein / Photosystem II reaction center protein Z / Cytochrome b559 subunit alpha ...Photosystem II D2 protein / Photosystem II CP47 reaction center protein / Photosystem II reaction center protein H / Cytochrome b559 subunit beta / Chlorophyll a-b binding protein, chloroplastic / Photosystem II reaction center protein K / Photosystem II reaction center protein I / Photosystem II CP43 reaction center protein / Photosystem II reaction center protein Z / Cytochrome b559 subunit alpha / Photosystem II reaction center protein T / Photosystem II protein D1 / Photosystem II reaction center protein Psb30 / Photosystem II reaction center protein L
Similarity search - Component
Biological speciesDunaliella salina (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsCaspy I / Fadeeva M
Funding support Israel, 2 items
OrganizationGrant numberCountry
Israel Science Foundation569/17 Israel
Israel Science Foundation199/21 Israel
CitationJournal: Elife / Year: 2023
Title: Structure of photosystem II reveals conformational flexibility of stacked and unstacked supercomplexes.
Authors: Ido Caspy / Maria Fadeeva / Yuval Mazor / Nathan Nelson /
Abstract: Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on ...Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on earth . Our knowledge on the mechanism of water photooxidation was greatly advanced by high-resolution structures of prokaryotic PSII . Here, we show high-resolution cryogenic electron microscopy (cryo-EM) structures of eukaryotic PSII from the green alga at two distinct conformations. The conformers are also present in stacked PSII, exhibiting flexibility that may be relevant to the grana formation in chloroplasts of the green lineage. CP29, one of PSII associated light-harvesting antennae, plays a major role in distinguishing the two conformations of the supercomplex. We also show that the stacked PSII dimer, a form suggested to support the organisation of thylakoid membranes , can appear in many different orientations providing a flexible stacking mechanism for the arrangement of grana stacks in thylakoids. Our findings provide a structural basis for the heterogenous nature of the eukaryotic PSII on multiple levels.
History
DepositionAug 22, 2021-
Header (metadata) releaseAug 17, 2022-
Map releaseAug 17, 2022-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13444.png

Downloads & links

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Map

FileDownload / File: emd_13444.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.96 Å/pix.
x 480 pix.
= 460.8 Å		0.96 Å/pix.
x 480 pix.
= 460.8 Å		0.96 Å/pix.
x 480 pix.
= 460.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.96 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.036
Minimum - Maximum-0.07208223 - 0.10197324
Average (Standard dev.)0.00005815569 (±0.008497476)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 460.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Dunaliella salina Photosystem II complex

EntireName: Dunaliella salina Photosystem II complex
Components
  • Complex: Dunaliella salina Photosystem II complex
    • Protein or peptide: Photosystem II protein D1
    • Protein or peptide: Photosystem II CP47 reaction center protein
    • Protein or peptide: Photosystem II reaction center protein Ycf12
    • Protein or peptide: Photosystem II CP43 reaction center protein
    • Protein or peptide: Photosystem II D2 protein
    • Protein or peptide: Cytochrome b559 subunit alpha
    • Protein or peptide: Cytochrome b559 subunit beta
    • Protein or peptide: Photosystem II reaction center protein H
    • Protein or peptide: Photosystem II reaction center protein I
    • Protein or peptide: PsbJ
    • Protein or peptide: Photosystem II reaction center protein K
    • Protein or peptide: Photosystem II reaction center protein L
    • Protein or peptide: PsbM
    • Protein or peptide: PsbO
    • Protein or peptide: PsbP
    • Protein or peptide: Photosystem II reaction center protein T
    • Protein or peptide: PsbW
    • Protein or peptide: PsbX
    • Protein or peptide: Photosystem II reaction center protein Z
    • Protein or peptide: LHCII M3
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: CP29
    • Protein or peptide: CP26
    • Protein or peptide: LHCII M1
    • Protein or peptide: PsbU
  • Ligand: CA-MN4-O5 CLUSTER
  • Ligand: FE (II) ION
  • Ligand: CHLORIDE ION
  • Ligand: CHLOROPHYLL A
  • Ligand: PHEOPHYTIN A
  • Ligand: BETA-CAROTENE
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: SPHINGOSINE
  • Ligand: SODIUM ION
  • Ligand: (1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE
  • Ligand: DIACYL GLYCEROL
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: trimethyl-[(2~{R})-1-oxidanyl-1-oxidanylidene-4-[(2~{S})-2-[(1~{S})-1-oxidanyloctadecoxy]-3-[(1~{R})-1-oxidanyloctadecoxy]propoxy]butan-2-yl]azanium
  • Ligand: BICARBONATE ION
  • Ligand: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: (3R)-beta,beta-caroten-3-ol
  • Ligand: GLYCEROL
  • Ligand: Tripalmitoylglycerol
  • Ligand: CHLOROPHYLL B
  • Ligand: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: ERGOSTEROL
  • Ligand: (2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate
  • Ligand: PHOSPHATIDYLETHANOLAMINE

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Supramolecule #1: Dunaliella salina Photosystem II complex

SupramoleculeName: Dunaliella salina Photosystem II complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#25
Source (natural)Organism: Dunaliella salina (plant)

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Macromolecule #1: Photosystem II protein D1

MacromoleculeName: Photosystem II protein D1 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO / EC number: photosystem II
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 37.291488 KDa
SequenceString: ENTSLWARFC EWITSTENRL YIGWFGVIMI PTLLTAISVY IIAFIAAPPV DIDGIREPVS GSLLYGNNII TGAVVPTSNA IGLHFYPIW EAASLDEWLY NGGPYQLVVC HFFLGVCCYM GREWELSYRL GMRPWIAVAY SAPVAAATAV FIIYPIGQGS F SDGMPLGI ...String:
ENTSLWARFC EWITSTENRL YIGWFGVIMI PTLLTAISVY IIAFIAAPPV DIDGIREPVS GSLLYGNNII TGAVVPTSNA IGLHFYPIW EAASLDEWLY NGGPYQLVVC HFFLGVCCYM GREWELSYRL GMRPWIAVAY SAPVAAATAV FIIYPIGQGS F SDGMPLGI SGTFNFMIVF QAEHNILMHP FHMFGVAGVF GGSLFSAMHG SLVTSSLIRE TTENESANAG YKFGQEEETY NI VAAHGYF GRLIFQYASF NNSRSLHFFL AVWPVVCIWL TALGISTMAF NLNGFNFNQS VVDSNGRVLN TWADIINRAN LGM EVMHER NAHNFPLDLA

UniProtKB: Photosystem II protein D1

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Macromolecule #2: Photosystem II CP47 reaction center protein

MacromoleculeName: Photosystem II CP47 reaction center protein / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 53.289516 KDa
SequenceString: GLPWYRVHTV VINDPGRLIS VHLMHTALVA GWAGAMTLFE IAVFDPSDPV LNPMWRQGMF VLPFLTRLGV TQSWGGWTIS GETSSNPGI WSYEGAAASH IVLSGLLFLA SVWHWVYWDL ELFRDPRTGK TALDLPKIFG IHLFLAGLLC FGFGAFHVTG V FGPGIWVS ...String:
GLPWYRVHTV VINDPGRLIS VHLMHTALVA GWAGAMTLFE IAVFDPSDPV LNPMWRQGMF VLPFLTRLGV TQSWGGWTIS GETSSNPGI WSYEGAAASH IVLSGLLFLA SVWHWVYWDL ELFRDPRTGK TALDLPKIFG IHLFLAGLLC FGFGAFHVTG V FGPGIWVS DPYGLTGSVQ PVAPSWGAEG FDPYNPGGVP AHHIAAGILG VLAGLFHLCV RPSIRLYFGL SMGSIESVLS SS IAAVFWA AFVVAGTMWY GSAATPIELF GPTRYQWDQG FFQQEIQKRV AQSTSEGLSV SEAWAKIPEK LAFYDYIGNN PAK GGLFRT GAMNSGDGIA VGWLGHASFK DQEGRELFVR RMPTFFETFP VVLIDKDGVV RADVPFRKAE SKYSIEQVGV SVTF YGGEL NGLTFTDPST VKKYARKAQL GEIFEFDRST LQSDGVFRSS PRGWFTFGHL SFALLFFFGH IWHGSRTIFR DVFAG IDED

UniProtKB: Photosystem II CP47 reaction center protein

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Macromolecule #3: Photosystem II reaction center protein Ycf12

MacromoleculeName: Photosystem II reaction center protein Ycf12 / type: protein_or_peptide / ID: 3 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 3.217949 KDa
SequenceString:
SLTLILQLVA LFAVVAAGPL VVVLLSVRGG NL

UniProtKB: Photosystem II reaction center protein Psb30

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Macromolecule #4: Photosystem II CP43 reaction center protein

MacromoleculeName: Photosystem II CP43 reaction center protein / type: protein_or_peptide / ID: 4 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 49.128992 KDa
SequenceString: GRDQETTGFA WWAGNARLIN LSGKLLGAHV AHAGLIVFWA GAMNLFEVSH FVPEKPMYEQ GLILLPHIAT LGYGVGPGGE VLDTFPYFV SGVLHLISSA VLGFGGVYHS LIGPETLEES YPFFGYVWKD KNKMTNILGY HLIILGCGAW LLVLKALYFG G VYDTWAPG ...String:
GRDQETTGFA WWAGNARLIN LSGKLLGAHV AHAGLIVFWA GAMNLFEVSH FVPEKPMYEQ GLILLPHIAT LGYGVGPGGE VLDTFPYFV SGVLHLISSA VLGFGGVYHS LIGPETLEES YPFFGYVWKD KNKMTNILGY HLIILGCGAW LLVLKALYFG G VYDTWAPG GGDVRIISNP TTNAAIIFGY IVKSPFGGDG WIVSVDNLED IIGGHIWIGT LCILGGIWHI YTTPWPWARR AF VWSGEAY LSYSLAAVSL MGFTACCFAW FNNTAYPSEF YGPTGPEASQ AQAFTFLVRD QRLGANVASA QGPTGLGKYL MRS PTGEII FGGETMRFWD FRGPWVEPLR GPSGLDLVKL KNDIQPWQER RAAEYMTHAP LGSLNSVGGV ATEINAVNFV SPRS WLATS HFCLGFFFFV GHLWHAGRAR AAAAGFEKGI DRVDEPVLSM RPLD

UniProtKB: Photosystem II CP43 reaction center protein

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Macromolecule #5: Photosystem II D2 protein

MacromoleculeName: Photosystem II D2 protein / type: protein_or_peptide / ID: 5 / Number of copies: 4 / Enantiomer: LEVO / EC number: photosystem II
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 38.965383 KDa
SequenceString: IGTYQEKRTW FDDADDWLRQ DRFVFVGWSG LLLLPCAYFA VGGWLTGCTF VTSWYTHGLA SSYIEGCNFL TAAVSTPANS LGHSLLFVW GPEAQGDLTR WFQLGGLWAF VALHGAFGLI GFMLRQFEIA RSVNLRPYNA IAFSAPIAVF VSVFLIYPLG Q SGWFFAPS ...String:
IGTYQEKRTW FDDADDWLRQ DRFVFVGWSG LLLLPCAYFA VGGWLTGCTF VTSWYTHGLA SSYIEGCNFL TAAVSTPANS LGHSLLFVW GPEAQGDLTR WFQLGGLWAF VALHGAFGLI GFMLRQFEIA RSVNLRPYNA IAFSAPIAVF VSVFLIYPLG Q SGWFFAPS FGVASIFRFI LFFQGFHNWT LNPFHMMGVA GVLGAALLCA IHGATVENTL FEDGDGANTF RAFNPTQAEE TY SMVTANR FWSQIFGVAF SNKRWLHFFM LFVPVTGLWM SALGVVGLAL NLRAYDFVSQ EIRAAEDPEF ETFYTKNILL NEG IRAWMA AQDQPHEKLT LPEEVLPRGN AL

UniProtKB: Photosystem II D2 protein

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Macromolecule #6: Cytochrome b559 subunit alpha

MacromoleculeName: Cytochrome b559 subunit alpha / type: protein_or_peptide / ID: 6 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 8.743847 KDa
SequenceString:
ERPFSDILTS IRYWVIHSIT VPSLFIAGWL FVSTGLAYDV FGSPRPNEYF TEDRQDAPLI TDRFNALEQV KKLSAQ

UniProtKB: Cytochrome b559 subunit alpha

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Macromolecule #7: Cytochrome b559 subunit beta

MacromoleculeName: Cytochrome b559 subunit beta / type: protein_or_peptide / ID: 7 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 3.549299 KDa
SequenceString:
IFTVRWLAIH AIAVPTIFFL GAITAMQFIQ R

UniProtKB: Cytochrome b559 subunit beta

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Macromolecule #8: Photosystem II reaction center protein H

MacromoleculeName: Photosystem II reaction center protein H / type: protein_or_peptide / ID: 8 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 7.124381 KDa
SequenceString:
EPGIVTPLGT LLRPLNSEAG KVLPGWGTTV LMAVAILLFA VFLLIILEIY NSSLILDGVT NSWESLA

UniProtKB: Photosystem II reaction center protein H

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Macromolecule #9: Photosystem II reaction center protein I

MacromoleculeName: Photosystem II reaction center protein I / type: protein_or_peptide / ID: 9 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 3.929648 KDa
SequenceString:
MLTLKIFVYT VVTFFVGLFI FGFLSNDPSR NPGKG

UniProtKB: Photosystem II reaction center protein I

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Macromolecule #10: PsbJ

MacromoleculeName: PsbJ / type: protein_or_peptide / ID: 10 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 3.7375 KDa
SequenceString:
IGRIPLWLVG TVVGLLAIGL LAIFFYGSYV GLGSSL

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Macromolecule #11: Photosystem II reaction center protein K

MacromoleculeName: Photosystem II reaction center protein K / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 4.159991 KDa
SequenceString:
KLPEAYAPFS PIVDVLPIIP VLFILLAFVW QASVSFR

UniProtKB: Photosystem II reaction center protein K

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Macromolecule #12: Photosystem II reaction center protein L

MacromoleculeName: Photosystem II reaction center protein L / type: protein_or_peptide / ID: 12 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 4.417203 KDa
SequenceString:
MARPNPNKQV VELNRSSLYW GLLLIFVLAV LFSSYIFN

UniProtKB: Photosystem II reaction center protein L

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Macromolecule #13: PsbM

MacromoleculeName: PsbM / type: protein_or_peptide / ID: 13 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 3.307979 KDa
SequenceString:
VNILGLTATA LFIIIPTSFL LILYVKTAAS E

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Macromolecule #14: PsbO

MacromoleculeName: PsbO / type: protein_or_peptide / ID: 14 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 25.874908 KDa
SequenceString: LTYDELQGLT YLQVKGTGIA NTCPVVEQGT SNVRELKPGD YKLEKFCMEP TSFTVKEEDA KGREEFVKTK LLTRLTYGLD GMNGSMKIN NDGSVEFRED EGLDYAATTV KLPGGEYVPF LFTIKEFDGK GTLDSFSGDF LVPSYRGSTF LDPKGRGGAT G YDNAIALP ...String:
LTYDELQGLT YLQVKGTGIA NTCPVVEQGT SNVRELKPGD YKLEKFCMEP TSFTVKEEDA KGREEFVKTK LLTRLTYGLD GMNGSMKIN NDGSVEFRED EGLDYAATTV KLPGGEYVPF LFTIKEFDGK GTLDSFSGDF LVPSYRGSTF LDPKGRGGAT G YDNAIALP AKADADEIQR LNNKSYTPGK GSAVFSVAKV DTETGEIAGV FESIQPSDTE MGAHPAKDIK ITGLWYGQLT

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Macromolecule #15: PsbP

MacromoleculeName: PsbP / type: protein_or_peptide / ID: 15 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 20.425707 KDa
SequenceString:
AYGEGANVFG RVTNKSGFVP YAGDSFALLL PSKWNPSDEK EVDSIVLRYA DNFDAVNNVY VIAQDTDKSS IQDYGSPEEF ISQFGYLLG RQAWAGQTVS EGGFDANKVS SAALLGVATE KDKKGKTYYK FEILSRTADG NEGGRHQLVK ATVSNGKLYL L KVQAGDKR WFKGTDKECL GVLDSFTVV

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Macromolecule #16: Photosystem II reaction center protein T

MacromoleculeName: Photosystem II reaction center protein T / type: protein_or_peptide / ID: 16 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 3.478194 KDa
SequenceString:
MEALVYTFLL IGTLGIIFFS IFFREPPRIA

UniProtKB: Photosystem II reaction center protein T

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Macromolecule #17: PsbW

MacromoleculeName: PsbW / type: protein_or_peptide / ID: 17 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 4.698315 KDa
SequenceString:
LVDDRMNGDG TGLPFGVNDG ILGWVIAGTL GTIWAIYFVS QKDL

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Macromolecule #18: PsbX

MacromoleculeName: PsbX / type: protein_or_peptide / ID: 18 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 2.854407 KDa
SequenceString:
SVTPSLKNFL LSLVAGAVVL AAIAGAVTAV

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Macromolecule #19: Photosystem II reaction center protein Z

MacromoleculeName: Photosystem II reaction center protein Z / type: protein_or_peptide / ID: 19 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 6.430616 KDa
SequenceString:
MTSILQIALL GLVLVSFALV VGVPVVFASP NGWTENKGVV FSGLSVWFLL VFAVGVFNSF A

UniProtKB: Photosystem II reaction center protein Z

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Macromolecule #20: LHCII M3

MacromoleculeName: LHCII M3 / type: protein_or_peptide / ID: 20 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 24.075082 KDa
SequenceString: TINQFYGPDR NKWLGPYSTN TPPYLTGEFP GDYGWDTAGL SADPETFKRY RELELIHARW ALLGALGMVT PELLQDDDGI MFGESAIWF KAGAAIFQEG GLNYLGNPSL IHAQNIVATL AVQVVLMGLV EGYRVNGGPA GEGLDPLYPG EAFDPLGLAD D PEAFAELK ...String:
TINQFYGPDR NKWLGPYSTN TPPYLTGEFP GDYGWDTAGL SADPETFKRY RELELIHARW ALLGALGMVT PELLQDDDGI MFGESAIWF KAGAAIFQEG GLNYLGNPSL IHAQNIVATL AVQVVLMGLV EGYRVNGGPA GEGLDPLYPG EAFDPLGLAD D PEAFAELK VKEIKNGRLA MFACLGFFVQ AIVTGKGPIE NLTDHLANPA ENNAFAYATK FTPQ

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Macromolecule #21: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 21 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 23.719543 KDa
SequenceString: EFYGPDRAKF LGPFSENDTP EYLTGEFPGD YGWDTAGLSA DPQTFARYRE IELIHARWAL LGALGILTPE LLSQYAGVQF GEPVWFKAG AQIFAAGGLN YLGNESLIHA QSIIATLAVQ VVLMGLAEAY RANGGSEGFL DDLDTLYPGG AFDPLGLADD P DTFAELKV ...String:
EFYGPDRAKF LGPFSENDTP EYLTGEFPGD YGWDTAGLSA DPQTFARYRE IELIHARWAL LGALGILTPE LLSQYAGVQF GEPVWFKAG AQIFAAGGLN YLGNESLIHA QSIIATLAVQ VVLMGLAEAY RANGGSEGFL DDLDTLYPGG AFDPLGLADD P DTFAELKV KEIKNGRLAM FSCLGFFVQA IVTGKGPVQN LTDHLADPTV NNAFASATKF TPG

UniProtKB: Chlorophyll a-b binding protein, chloroplastic

+
Macromolecule #22: CP29

MacromoleculeName: CP29 / type: protein_or_peptide / ID: 22 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 21.163662 KDa
SequenceString: SLSYLDGHLP GDMGFDPLHL SSPTV(SEP)LQIG VDEEDQNQAQ NKKGDVEAIF RPEVFGLARF RETEVIHGRW AMLGTL GAI VGEAATGVSW VDAGKVELDG AQYLGQSLPF SISQLIWIEA ILVGGVEVLR NNELDLEKRI YPGGAFDPLN LADEEDE EK SFRLKTAEIK ...String:
SLSYLDGHLP GDMGFDPLHL SSPTV(SEP)LQIG VDEEDQNQAQ NKKGDVEAIF RPEVFGLARF RETEVIHGRW AMLGTL GAI VGEAATGVSW VDAGKVELDG AQYLGQSLPF SISQLIWIEA ILVGGVEVLR NNELDLEKRI YPGGAFDPLN LADEEDE EK SFRLKTAEIK HGRLAMVAFL GFGIQAAATG EGALGSLA

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Macromolecule #23: CP26

MacromoleculeName: CP26 / type: protein_or_peptide / ID: 23 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 26.233607 KDa
SequenceString: PDLEKWYGPD RKLFLPGGLL DRDDVPEYLN GELAGDYGYD PLGLGKDEEQ VEKYRANELL HARWAMLAAA GIIIPEGLQA NGADIKGGT WFETGAEMLN GGTLNYFAVP FGVVQNPLPL AAVTLIEIVL LGAVEKFRVD GTGPAGFSPG VGKFDSDIFD G LDKLYPGG ...String:
PDLEKWYGPD RKLFLPGGLL DRDDVPEYLN GELAGDYGYD PLGLGKDEEQ VEKYRANELL HARWAMLAAA GIIIPEGLQA NGADIKGGT WFETGAEMLN GGTLNYFAVP FGVVQNPLPL AAVTLIEIVL LGAVEKFRVD GTGPAGFSPG VGKFDSDIFD G LDKLYPGG PFDPLNLADD PEVFAELQVK EIKNGRLAMI AVLAFAIQSY VTGEGPYANI TKHLSDPFGY NLLTVLQGDD RV PTL

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Macromolecule #24: LHCII M1

MacromoleculeName: LHCII M1 / type: protein_or_peptide / ID: 24 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 23.537566 KDa
SequenceString: SAWYGPDRPL FLGSLSGEPP SYLTGEFPGD YGWDTAGLSA DPTTFARYRT IELIHARWAL LGALGIITPE LLAKNGVPFS EDGAIWFKA GAEIFKEGGL NYLGNENLIH AQSILATLAV QVIVMGAAEG FRANGEAPGV EGLDPLYPGG PFDPLGLADD P EAFAELKV ...String:
SAWYGPDRPL FLGSLSGEPP SYLTGEFPGD YGWDTAGLSA DPTTFARYRT IELIHARWAL LGALGIITPE LLAKNGVPFS EDGAIWFKA GAEIFKEGGL NYLGNENLIH AQSILATLAV QVIVMGAAEG FRANGEAPGV EGLDPLYPGG PFDPLGLADD P EAFAELKV KEIKNGRLAM FSCLGFFVQA IVTGKGPIQN LQDHLADPGV NNAFASATKF VPTP

+
Macromolecule #25: PsbU

MacromoleculeName: PsbU / type: protein_or_peptide / ID: 25 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Dunaliella salina (plant)
Molecular weightTheoretical: 3.219625 KDa
SequenceString:
QRVRTVLDMD DPAKEETVKE LRKDINN

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Macromolecule #26: CA-MN4-O5 CLUSTER

MacromoleculeName: CA-MN4-O5 CLUSTER / type: ligand / ID: 26 / Number of copies: 4 / Formula: OEX
Molecular weightTheoretical: 339.827 Da
Chemical component information

ChemComp-OEX:
CA-MN4-O5 CLUSTER

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Macromolecule #27: FE (II) ION

MacromoleculeName: FE (II) ION / type: ligand / ID: 27 / Number of copies: 4 / Formula: FE2
Molecular weightTheoretical: 55.845 Da

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Macromolecule #28: CHLORIDE ION

MacromoleculeName: CHLORIDE ION / type: ligand / ID: 28 / Number of copies: 8 / Formula: CL
Molecular weightTheoretical: 35.453 Da

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Macromolecule #29: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 29 / Number of copies: 312 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A

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Macromolecule #30: PHEOPHYTIN A

MacromoleculeName: PHEOPHYTIN A / type: ligand / ID: 30 / Number of copies: 8 / Formula: PHO
Molecular weightTheoretical: 871.2 Da
Chemical component information

ChemComp-PHO:
PHEOPHYTIN A

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Macromolecule #31: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 31 / Number of copies: 32 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE

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Macromolecule #32: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 32 / Number of copies: 20 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Macromolecule #33: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 33 / Number of copies: 28 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

+
Macromolecule #34: SPHINGOSINE

MacromoleculeName: SPHINGOSINE / type: ligand / ID: 34 / Number of copies: 8 / Formula: SPH
Molecular weightTheoretical: 299.492 Da
Chemical component information

ChemComp-SPH:
SPHINGOSINE

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Macromolecule #35: SODIUM ION

MacromoleculeName: SODIUM ION / type: ligand / ID: 35 / Number of copies: 4
Molecular weightTheoretical: 22.99 Da

+
Macromolecule #36: (1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},...

MacromoleculeName: (1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca- ...Name: (1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol
type: ligand / ID: 36 / Number of copies: 4 / Formula: C7Z
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-C7Z:
(1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol

+
Macromolecule #37: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 37 / Number of copies: 16 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

+
Macromolecule #38: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

MacromoleculeName: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / type: ligand / ID: 38 / Number of copies: 12 / Formula: 3PH
Molecular weightTheoretical: 704.998 Da
Chemical component information

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

+
Macromolecule #39: DIACYL GLYCEROL

MacromoleculeName: DIACYL GLYCEROL / type: ligand / ID: 39 / Number of copies: 12 / Formula: DGA
Molecular weightTheoretical: 625.018 Da
Chemical component information

ChemComp-DGA:
DIACYL GLYCEROL

+
Macromolecule #40: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 40 / Number of copies: 35 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

+
Macromolecule #41: trimethyl-[(2~{R})-1-oxidanyl-1-oxidanylidene-4-[(2~{S})-2-[(1~{S...

MacromoleculeName: trimethyl-[(2~{R})-1-oxidanyl-1-oxidanylidene-4-[(2~{S})-2-[(1~{S})-1-oxidanyloctadecoxy]-3-[(1~{R})-1-oxidanyloctadecoxy]propoxy]butan-2-yl]azanium
type: ligand / ID: 41 / Number of copies: 4 / Formula: LMK
Molecular weightTheoretical: 773.241 Da
Chemical component information

ChemComp-LMK:
trimethyl-[(2~{R})-1-oxidanyl-1-oxidanylidene-4-[(2~{S})-2-[(1~{S})-1-oxidanyloctadecoxy]-3-[(1~{R})-1-oxidanyloctadecoxy]propoxy]butan-2-yl]azanium

+
Macromolecule #42: BICARBONATE ION

MacromoleculeName: BICARBONATE ION / type: ligand / ID: 42 / Number of copies: 4 / Formula: BCT
Molecular weightTheoretical: 61.017 Da
Chemical component information

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

+
Macromolecule #43: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...

MacromoleculeName: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
type: ligand / ID: 43 / Number of copies: 4 / Formula: PL9
Molecular weightTheoretical: 749.201 Da
Chemical component information

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE

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Macromolecule #44: PROTOPORPHYRIN IX CONTAINING FE

MacromoleculeName: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 44 / Number of copies: 4 / Formula: HEM
Molecular weightTheoretical: 616.487 Da
Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

+
Macromolecule #45: (3R)-beta,beta-caroten-3-ol

MacromoleculeName: (3R)-beta,beta-caroten-3-ol / type: ligand / ID: 45 / Number of copies: 4 / Formula: RRX
Molecular weightTheoretical: 552.872 Da
Chemical component information

ChemComp-RRX:
(3R)-beta,beta-caroten-3-ol

+
Macromolecule #46: GLYCEROL

MacromoleculeName: GLYCEROL / type: ligand / ID: 46 / Number of copies: 2 / Formula: GOL
Molecular weightTheoretical: 92.094 Da
Chemical component information

ChemComp-GOL:
GLYCEROL

+
Macromolecule #47: Tripalmitoylglycerol

MacromoleculeName: Tripalmitoylglycerol / type: ligand / ID: 47 / Number of copies: 8 / Formula: 4RF
Molecular weightTheoretical: 807.32 Da
Chemical component information

ChemComp-4RF:
Tripalmitoylglycerol

+
Macromolecule #48: CHLOROPHYLL B

MacromoleculeName: CHLOROPHYLL B / type: ligand / ID: 48 / Number of copies: 92 / Formula: CHL
Molecular weightTheoretical: 907.472 Da
Chemical component information

ChemComp-CHL:
CHLOROPHYLL B

+
Macromolecule #49: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

MacromoleculeName: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 49 / Number of copies: 36 / Formula: LUT
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

+
Macromolecule #50: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...

MacromoleculeName: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 50 / Number of copies: 16 / Formula: XAT
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

+
Macromolecule #51: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...

MacromoleculeName: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
type: ligand / ID: 51 / Number of copies: 20 / Formula: NEX
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

+
Macromolecule #52: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 52 / Number of copies: 4 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

+
Macromolecule #53: ERGOSTEROL

MacromoleculeName: ERGOSTEROL / type: ligand / ID: 53 / Number of copies: 4 / Formula: ERG
Molecular weightTheoretical: 396.648 Da
Chemical component information

ChemComp-ERG:
ERGOSTEROL

+
Macromolecule #54: (2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypro...

MacromoleculeName: (2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate
type: ligand / ID: 54 / Number of copies: 4 / Formula: LPX
Molecular weightTheoretical: 453.55 Da
Chemical component information

ChemComp-LPX:
(2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate

+
Macromolecule #55: PHOSPHATIDYLETHANOLAMINE

MacromoleculeName: PHOSPHATIDYLETHANOLAMINE / type: ligand / ID: 55 / Number of copies: 8 / Formula: PTY
Molecular weightTheoretical: 734.039 Da
Chemical component information

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 6.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number real images: 13586 / Average electron dose: 51.81 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 401467
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 9567
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial modelPDB ID:

6kac


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementProtocol: AB INITIO MODEL
Output model

PDB-7pin:
Stacked compact Dunaliella PSII

+
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