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- EMDB-12742: Cryo-EM structure of a respiratory complex I -

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Basic information

Entry
Database: EMDB / ID: EMD-12742
TitleCryo-EM structure of a respiratory complex I
Map datacomplex I map postprocessed in relion (2.4 angstrom resolution)
Sample
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: x 42 types
  • Ligand: x 13 types
Function / homology
Function and homology information


NADH dehydrogenase / oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / respiratory chain complex I / : / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / quinone binding ...NADH dehydrogenase / oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / respiratory chain complex I / : / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / quinone binding / : / respiratory electron transport chain / mitochondrial membrane / electron transport chain / mitochondrial intermembrane space / fatty acid biosynthetic process / 2 iron, 2 sulfur cluster binding / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / mitochondrial inner membrane / oxidoreductase activity / mitochondrion / membrane / metal ion binding
Similarity search - Function
NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 ...NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NDUFA6, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / : / NADH-ubiquinone oxidoreductase subunit G, C-terminal / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / SLBB domain / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / Complex 1 LYR protein domain / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / Complex 1 protein (LYR family) / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / : / NADH-quinone oxidoreductase, subunit D / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH ubiquinone oxidoreductase, F subunit / NADH dehydrogenase, subunit C / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH:ubiquinone oxidoreductase / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / Proton-conducting membrane transporter / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / 2Fe-2S iron-sulfur cluster binding domain / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / NAD-dependent epimerase/dehydratase / NAD dependent epimerase/dehydratase family / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / CHCH
Similarity search - Domain/homology
Complex 1 LYR protein domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Uncharacterized protein / Acyl carrier protein ...Complex 1 LYR protein domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Uncharacterized protein / Acyl carrier protein / Uncharacterized protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-domain-containing protein / Uncharacterized protein / Thioredoxin-like protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein / Uncharacterized protein / Zinc finger CHCC-type domain-containing protein / NAD-dependent epimerase/dehydratase domain-containing protein / NADH dehydrogenase 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase / ETC complex I subunit conserved region-domain-containing protein / Uncharacterized protein / Uncharacterized protein / NUWM protein / NUVM protein / Subunit NUKM of protein NADH:Ubiquinone oxidoreductase / Subunit NUIM of protein NADH:Ubiquinone oxidoreductase / Subunit NUHM of protein NADH:Ubiquinone oxidoreductase / NUGM protein / NUCM protein / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUAM protein / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 4L
Similarity search - Component
Biological speciesYarrowia lipolytica (yeast) / Candida lipolytica (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.4 Å
AuthorsParey K / Vonck J
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research Foundation (DFG)ZI 552/4-2 Germany
CitationJournal: Sci Adv / Year: 2021
Title: High-resolution structure and dynamics of mitochondrial complex I-Insights into the proton pumping mechanism.
Authors: Kristian Parey / Jonathan Lasham / Deryck J Mills / Amina Djurabekova / Outi Haapanen / Etienne Galemou Yoga / Hao Xie / Werner Kühlbrandt / Vivek Sharma / Janet Vonck / Volker Zickermann /
Abstract: Mitochondrial NADH:ubiquinone oxidoreductase (complex I) is a 1-MDa membrane protein complex with a central role in energy metabolism. Redox-driven proton translocation by complex I contributes ...Mitochondrial NADH:ubiquinone oxidoreductase (complex I) is a 1-MDa membrane protein complex with a central role in energy metabolism. Redox-driven proton translocation by complex I contributes substantially to the proton motive force that drives ATP synthase. Several structures of complex I from bacteria and mitochondria have been determined, but its catalytic mechanism has remained controversial. We here present the cryo-EM structure of complex I from at 2.1-Å resolution, which reveals the positions of more than 1600 protein-bound water molecules, of which ~100 are located in putative proton translocation pathways. Another structure of the same complex under steady-state activity conditions at 3.4-Å resolution indicates conformational transitions that we associate with proton injection into the central hydrophilic axis. By combining high-resolution structural data with site-directed mutagenesis and large-scale molecular dynamic simulations, we define details of the proton translocation pathways and offer insights into the redox-coupled proton pumping mechanism of complex I.
History
DepositionApr 12, 2021-
Header (metadata) releaseNov 10, 2021-
Map releaseNov 10, 2021-
UpdateDec 1, 2021-
Current statusDec 1, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.016
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.016
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7o71
  • Surface level: 0.016
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_12742.png

Downloads & links

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Map

FileDownload / File: emd_12742.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcomplex I map postprocessed in relion (2.4 angstrom resolution)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.52 Å/pix.
x 600 pix.
= 309.6 Å		0.52 Å/pix.
x 600 pix.
= 309.6 Å		0.52 Å/pix.
x 600 pix.
= 309.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.516 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.016 / Movie #1: 0.016
Minimum - Maximum-0.044632785 - 0.10371717
Average (Standard dev.)9.1163434e-05 (±0.0034132805)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 309.59998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.5160.5160.516
M x/y/z600600600
origin x/y/z0.0000.0000.000
length x/y/z309.600309.600309.600
α/β/γ90.00090.00090.000
start NX/NY/NZ7810892
NX/NY/NZ127111124
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS600600600
D min/max/mean-0.0450.1040.000

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Supplemental data

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Mask #1

Fileemd_12742_msk_1.map
Projections & Slices
AxesZYX

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Additional map: complex I map after density modification by resolve cryo em

Fileemd_12742_additional_1.map
Annotationcomplex I map after density modification by resolve_cryo_em
Projections & Slices
AxesZYX

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Half map: half map 1

Fileemd_12742_half_map_1.map
Annotationhalf map 1
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AxesZYX

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Half map: half map 2

Fileemd_12742_half_map_2.map
Annotationhalf map 2
Projections & Slices
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Sample components

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Entire : Mitochondrial NADH:ubiquinone oxidoreductase

EntireName: Mitochondrial NADH:ubiquinone oxidoreductase
Components
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: NADH-ubiquinone oxidoreductase 78 kDa subunit
    • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
    • Protein or peptide: NUCM protein
    • Protein or peptide: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
    • Protein or peptide: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
    • Protein or peptide: NADH dehydrogenase subunit 2
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
    • Protein or peptide: subunit NI9M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase 40 kDa subunit
    • Protein or peptide: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUMM of protein NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUYM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: DIUNDECYL PHOSPHATIDYL CHOLINE
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: Lauryl Maltose Neopentyl Glycol
  • Ligand: Phosphatidylinositol
  • Ligand: 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: CARDIOLIPIN
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: ZINC ION
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: water

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Supramolecule #1: Mitochondrial NADH:ubiquinone oxidoreductase

SupramoleculeName: Mitochondrial NADH:ubiquinone oxidoreductase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#42
Source (natural)Organism: Yarrowia lipolytica (yeast) / Strain: GB30
Molecular weightTheoretical: 1 MDa

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Macromolecule #1: NADH-ubiquinone oxidoreductase 78 kDa subunit

MacromoleculeName: NADH-ubiquinone oxidoreductase 78 kDa subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 79.088078 KDa
SequenceString: MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE ...String:
MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE DKNIGPLVKT SMNRCIHCTR CVRFANDIAG APELGSSGRG NDMQIGTYLE KNLNTELSGN VIDLCPVGAL TN KPYAFRA RPWELKKTES IDVMDAVGSN IRIDSKGVEV MRVIPRVHED VNEEWINDKS RFACDGLKTQ RLTTPLIRVG DKF VNATWD DALSTIAKAY QQKAPKGDEF KAVAGALVEV ESMVALKDMT NALGSENTTT DTPNGNSAPA HGITFRSNYL FNSS IAGIE DADAILLVGT NPRREAAVMN ARIRKAWLRQ ELEIASVGPT LDATFDVAEL GNTHADLEKA LSGEFGEVLK NAKNP LIIV GSGITDREDA GAFFNTIGKF VESTPSVLNE NWNGYNVLQR SASRAGAYDI GFTPSDEASK TTPKMVWLLG ADEVAA SDI PADAFVVYQG HNGDVGAQFA DVVLPGAAYT EKAGTYVNTE GRSQISRAAT GPPGGAREDW KILRAVSEYL GVALPYE DA YEVRDRLAEI SPSLVRYDLV EPTVFGDVAV QHSLVGPNGS VTPSSAPLTE TIENFYMTDS ISRSSPTMAK SSIAFNKD N KKNQAFA

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Macromolecule #2: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 53.829508 KDa
SequenceString: MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA ...String:
MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA AVLQTAINEA YAAGLIGKDA CGSGYDFDVY IHRGMGAYVC GEETSLIESL EGKAGKPRLK PPFPAGVGLF GR PSTVTNV ETVAVAPTIL RRGGDWFASF GRERNSGTKL FCISGNVNEP CTVEEEMSIP LRELLEKHCG GIKGGWDNLL GVI PGGCSV PILPKNICED VLMDFDALKD VQSGLGTAAV IVINKQQDVI RAIQRFAAFY KHESCGQCTP CREGTTWLLK AMDR FRTGQ AKEREIDMLY ELTKDIEGHT ICALGDAAAW PIQGLIRNFR PEMETRMKKF HDEVGAVSVG GWMKDARVEK GKVVG APLP GVHH

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Macromolecule #3: NUCM protein

MacromoleculeName: NUCM protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 52.521949 KDa
SequenceString: MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL H(2MR)GTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSL AV EKLLNVEVPL ...String:
MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL H(2MR)GTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSL AV EKLLNVEVPL RGKYIRTMFG EITRVLNHLM SVCSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA YVRPGGVS Q DLPAGLLDDI YMWATQFGDR LDEIEELLTD NRIWKLRTVN IGTVTAQDAL NLGLSGPMLR GSGIPFDIRK NAPYDAYDK VDFDVPVGMN GDCYDRYLIR MAEFRQSLRI IEQCCNDMPA GAVKVEDFKI NSPPRNLMKE DMEALIHHFL LYTKGYSVPP GETYTAIEA PKGEMGVYVV SDGSERPYKC KIRAPGFAHL GAFDHIARGH FLPDAVAIIG TMDLVFGEVD R

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Macromolecule #4: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 32.389568 KDa
SequenceString: MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI ...String:
MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI TCLYEGANWF EREAYDMYGV FFEGHPDLRR IMTDYGFEGH PLRKDFPLTG YTEVRWDEEK RRVVYEPLEL TQ AFRNFSA GSTAWEPVGP GRDDRPDSFK LPTPKPEEKE GDKK

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Macromolecule #5: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 27.247402 KDa
SequenceString: MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC ...String:
MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC LGACVNAPMM AINDDYYEDL TPEGTVKLLE DCKAGKMPTP GPENHVRRDC EPASGQKVLL SKEPHNVADF LQ EGI

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Macromolecule #6: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 25.682305 KDa
SequenceString: MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK ...String:
MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK CIYCGYCQES CPVDAIVETP NVEYATETRE ELLYNKEKLL ANGDKWELEL QYALDADAPY R

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Macromolecule #7: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 23.455176 KDa
SequenceString: MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD ...String:
MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD RIVPVDVYVP GCPPTSEALM YGVFQLQRKM RNTKITRMWY RK

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Macromolecule #8: NADH-ubiquinone oxidoreductase chain 4L

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.84386 KDa
SequenceString:
(FME)FIGTIILVL SFLGFVFNRR NIILAFICLE TMLLGINLIL LRNSVLFDDI SGSLFAIVII ILAGVESAIG LSLLVS YYR LRGVINSYGI

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Macromolecule #9: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 28.479941 KDa
SequenceString: MLKLHYRNFI TAQHSTTNTT PTMIASVCKR AGLRAGPRAY PGVRQFALRA YNEEKELALK QRLSQLPPPG KAFVTAEGEP RPAKEAELA ELAEIAALYK TDRVGILDIL LLGNKHARLY RDNTALLKDY YYNGRRILDK IPVKDKQTGK VTWEIKREGA E KEDWVNQM ...String:
MLKLHYRNFI TAQHSTTNTT PTMIASVCKR AGLRAGPRAY PGVRQFALRA YNEEKELALK QRLSQLPPPG KAFVTAEGEP RPAKEAELA ELAEIAALYK TDRVGILDIL LLGNKHARLY RDNTALLKDY YYNGRRILDK IPVKDKQTGK VTWEIKREGA E KEDWVNQM YFLYAPSLIL LLIVMVYKSR EDITFWAKKE LDQRVLDKHP EINDAPENER DALIVERIIA GDYDKLASLQ KK ATPTPAT LI

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Macromolecule #10: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 10.494885 KDa
SequenceString:
MVELKPSSAI QRGPLNKGGW DAPHALHNDG AIDRYAHWRT FYQERFKYTR ATGKSTLIFL VAFPALIGYV AYQSEGLFEF AGKRRGESV TTRG

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Macromolecule #11: NADH-ubiquinone oxidoreductase chain 1

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 38.389277 KDa
SequenceString: (FME)IINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVIS PLI TLITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFII II MFVSSLNITT ...String:
(FME)IINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVIS PLI TLITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFII II MFVSSLNITT IIETQRVVWY CIPLLPLLLI FFIASVAETA RPPFDLTESE SELVAGYFTE YSGSPFVFFF LAEYSNII L ISAFNGYLLL GGYLSFNYSY LFNILFNDYS YVSFLFEGLI NSSAYAIKLV FLMFSFIWVR AAFPRFTYDN LINFCWIIL LPLLFGIFLI IPSTLYIFDS FPTLI

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Macromolecule #12: NADH dehydrogenase subunit 2

MacromoleculeName: NADH dehydrogenase subunit 2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 53.381367 KDa
SequenceString: (FME)LILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIM IVI SSLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASML YF FMGGILSILI ...String:
(FME)LILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIM IVI SSLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASML YF FMGGILSILI AYSINTYYSV LNSYTLHSLD SLIINTLDLN LILIALSLGL LFKIGIAPLH KWLISIYENT PILITIYI S LIPKISILSY LVLSNISINS LVISILAILT LLVGSVGGLL QIKIKRLLAF SGLTNAGYMM LLLLLNNNEF SYLYYITQY SISHLAIFMI IIFSIYYINY INNQYNPIIY VNQLKGLIHD NAYLVLSMAI VVFSFIGIPP LLGFFGKLNI LMSILNNGYY FISIVLIVA SLISALYYLY LLNVSIQDKN NILINSNETV SSVLSYILSS LIILITFGFI YNSLIIDIFN VYFN

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Macromolecule #13: NADH-ubiquinone oxidoreductase chain 3

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.506339 KDa
SequenceString:
(FME)NTFIIFIIL IPIVGFALLA VNILLAVYKP YNEKLGAFEC GLTSFNQTRL AFNAAFILVA ILFLPFDLEI STLLPY VMS IYLVSNYGFT IVLLFLLILI IGFVYEINTN ALKINKHNKP NTDSLIYKL

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Macromolecule #14: NADH-ubiquinone oxidoreductase chain 4

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 54.534652 KDa
SequenceString: (FME)FLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNG LLF GIDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYG SS DSERASFYVL ...String:
(FME)FLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNG LLF GIDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYG SS DSERASFYVL MFTLSGSLFM LLSIVVISIV LNTTNFINHN LFVLSLDLQT IIWLGLFIAI MVKTPLFPIH VWLPVVHS E SPLAGSMILA GLILKLALYA ILRLLLPLLC EAQILYTPMI YIISLLTIIL TSLATLRQID LKVIIAYSSI SHMGIAILG VCSNTSLGIY GSIVLGVAHG FVSPALFLIV GGILYDRYHI RIVNYYKGLT TYMPQLATYI IILSFANIGT PLTGNFTGEF LSLQGGFIR NPIIGGISCI SVLLAAIYQL KLTNKLTGGI SSIYMHRTND VTIREKFIMN ILIISTLIIG ICPQIMYNLL Y WTVNNYIY II

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Macromolecule #15: NADH-ubiquinone oxidoreductase chain 5

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 73.768703 KDa
SequenceString: (FME)YNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDIN YNF EIDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISF WV TRLQAMKSAL ...String:
(FME)YNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDIN YNF EIDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISF WV TRLQAMKSAL SAVLMNRFGD AFFVLGLCVI AYVFGTLNYS TIFATAYLIN TDLLVLIMLA LFIAAMAKSA QFGLHNWL T LAMEGPTPVS SLLHAATLVT AGIYLLLRSA NILEYTPTVL FIILWIGALT TLSAGLIAIC SNDLKRIIAL STMSQLGMM TIAIGLSAYN LALFHLLGHA FFKALLFMSA GSIIHSILNE SQDIRTYGGL LSYLPYTYIC ITIASLSLMA MPGLTGYYTK DIIIESTYG SYSISNYVVY WIAYLSAVLT CVYSMKILYL TFYSNPNNNT ITYYNAHESN IYITLPMFIL AIFAMFAGWI L KDIYLGVG TDFVGTHILP NNFSYFDTEF SITQFYKLLP LISAILVSIL IVVLNEFFAI VFNLNNKYIN TVYSIFNQKL VS DQILNHF IIFKGLVTSG NIAHHVDKGS LYRLGPVGIN RLLNKASYNV INLSSNTRSS LSMNSMLILI TIVSLLLLVL VMN VNFIIV IPVLISILYI LFS

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Macromolecule #16: NADH-ubiquinone oxidoreductase chain 6

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 20.793111 KDa
SequenceString:
(FME)MYLTYYFIE ITIFLAILCT IFIISAKNPM VSILYMIALF VIAAMYLYLI GLGIFSLLYI MIYIGAIAVL FLFIIT LLD INSTELSVKS NIRDLPLVLI SLIVLTISGL MIYSNDSILI NKLLEAFGND YNTIITQDWF NIENTTLLTT IGNVLLT NN AFILLVLAIV LLLGIIGPIS ITMKHKE

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Macromolecule #17: subunit NI9M of protein NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: subunit NI9M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 8.992229 KDa
SequenceString:
MINANPGFWN GPFRYLRWSA HNRPHLFFAF AIGIAGPVAA LTLTPLRRKY LYPDHSPLPQ SYPLPQRARE QLTGFDDE

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Macromolecule #18: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 9.806238 KDa
SequenceString:
MAIPFEALLP YGIIFGLLTA GGGAMQVLHV YRNGGVRDRF AIDQWDSQMM ERDLRLNGGQ GRKQVDQATA PEAFKHNHVW KSERPLI

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Macromolecule #19: NADH-ubiquinone oxidoreductase 40 kDa subunit

MacromoleculeName: NADH-ubiquinone oxidoreductase 40 kDa subunit / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 42.765738 KDa
SequenceString: MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA ...String:
MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA FNAEIDSPSE FNHTKGLGEQ VTKDIVPWAT IVRPAPMFGR EDKWFLDRMA RSPCLVSANK FQETSNPVHV ID VAAALER ICFDDSTVAQ TFELYGPQKF TQKQIIDMVS ETLRKEVRHI ELPKALYQAY TKATQAIWWP TYSPDQVERQ FLS QKIDPS AKTFNDLDLT PMELPDLMFK LIRPYRVNTF QHDVSQLENK EKTFVHILD

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Macromolecule #20: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.65792 KDa
SequenceString:
MRYTQLLRNV SKGVQKSGQS VLVQMREGTP IGLTGIYQHP NPRPALIALY EATLKELQDK HPKDSVYRQS IENLTAHRKQ IVEDNEVSE VIENKIGAGL IEEVVIQAHE ELELAKKMSE WKPWEELEEK PLEDQWVYFN KKGVE

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Macromolecule #21: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 20.849551 KDa
SequenceString: MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH ...String:
MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH RRPLSLTVEQ LGEGRGINAV PIATATEAPN

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Macromolecule #22: Subunit NUMM of protein NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUMM of protein NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 15.14897 KDa
SequenceString:
MLSRFVSKRA FSVSTQVSKS IISYNGNTIE IPEEYTKQAP NRDSTWAPAQ ASKTEIYKNN MVRFEQKDLS KQPMPYAGIE LIAQQPVRF VHGNTAVCDG ANHNGPGAQG HPKIFINVDA PGSHACQYCG TRYEKEH

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Macromolecule #23: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Com...

MacromoleculeName: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 12.053585 KDa
SequenceString:
MLRNVSRLAL RSNFARQATM GQRFYSVARP DAEKRIAAVL ESFDKISNPA AITPTASFAK DLNLDSLDTV EVVVAIEEEF GIEIPDKEA DEIKSVNQAV EYILAQPDAK

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Macromolecule #24: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 14.778818 KDa
SequenceString:
MAIIATAFAE TVKFSGSKQE LQKRTLALYR QFLRGAPTFA DLYEVQFSIP TIRTKIRQEF ERHRFVDDLS IQNVLYAKGH MEYQECINF WKQQAQFLKY FPEEDDIQGR HQPSNFVDKF LKNRA

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Macromolecule #25: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Com...

MacromoleculeName: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 14.444458 KDa
SequenceString:
MLRQSVLRLS RAAVARPALS RSFVTAVARP QLVRAAPVSF IRHYSSAHVL TKDMIQERIV ALLESFDKVN DAKNITATAN LTSDLGLDS LDVVEVVMAI EEEFGLEIPD HDADEIKTVQ QAIDYVSAQP AAV

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Macromolecule #26: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 12.90287 KDa
SequenceString:
MSHPVPFSAA NKKLVTSMYR QSLKLARNWI SNRQLFRQKA VEIRHKFDQN AQISNPRLLA RTLDETRAHL YEFRHPDPIV PPSFPGGTK YERNVPPRME KIMQHNLYEP

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Macromolecule #27: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 19.355197 KDa
SequenceString:
MPREAAAHWV PFEDKANMPD NVPDVVEVGA TSAPLLSASY FIGAKCKPYN DDFMLCREES QGSGAIDCLK EGRRVTRCAV SVIEDINKS CLDEFRLHWQ CLEQNNHQLS GCRKAEALLN KCVFTKLNLE KKIPGLRPDE EPVFLKKDPW IKPAVDDFKS V RAYAEAKK NGTL

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Macromolecule #28: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 14.112429 KDa
SequenceString:
MPSVGQDLPP VGGYEPVQWR RNLPARGFRP LVYLAALCGI CGYGFYRALG GIQERRELKR EKLWARIYLM PLLQAEEDRQ TVRRSIAQL EREKEIMKGT GFDVDKSVYN DGKFHAPALM IPPK

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Macromolecule #29: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 18.588209 KDa
SequenceString:
MSSSTPLVKT SVNYSYGDYP LIDADPHFKR VVGYMRPSDY GVIGLATAAL PAGICFAEWL DPVKGKFARP SVKFLRVATM LGFAVGFGA AYARSSLRFF GVTENAREYK KDEAQMAARK AAGLEPYGTS SLTPELQEIA AKNSAHSIAG LFIFPWFNFV N HPYHGREQ K

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Macromolecule #30: Subunit NUYM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUYM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 18.65608 KDa
SequenceString:
MLSRSLRQLS QPSVRSFATS ARLLQKKDVP EVGVNLDNVP AHEIVSGAPA ELSRNRVVRI YQQAKPATQS GEYGTFAWRL DWDIVDVAN RWENDLIGWQ SSGDYMQATQ MKFTSKESAI KFANKQGWDF YIQEPHHRKF RVKQYANNFV HSYGKLKHIR T K

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Macromolecule #31: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 19.772525 KDa
SequenceString:
MLPGGPVPVF KKYTVGSKGI WEKLRVLLAI APNRSTGNPI VPLYRVPTPG SRPEANVYQD PSSYPTNDIA ENPYWKRDHR RAYPQTAFF DQKTVTGLLE LGSEATPRIA DGEAGTKALA NIANGGVSFT QALGKSSKDV IYGEVLTVNG LPPVAPTLAP K QWKIIEGE AAIYPKGYPC RTFH

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Macromolecule #32: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 17.328523 KDa
SequenceString:
MLSRRAVTML RSPVARVAQV QVRGIRASFD KAEEPMLGDY PDIDPFPAQL KNPYKKYDDQ QDRRNLEEPL SVNDDLYDMW SPDRFTHFK NQDALKYFIG FLTIFFGASY VATYFVPEKA AIPREFPYEG LWKESGGTEK SKAFFGQRSE

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Macromolecule #33: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 8.059366 KDa
SequenceString:
MALFTSLVGA SGLGFATKFL SNKIRLKPAG YYPLGYVFSG VAWAGLGLVL HNVHQHSLEV LEKKKTALSE QRTE

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Macromolecule #34: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 6.948902 KDa
SequenceString:
MAPQLKDPWA RREAWRYQTN FTRANRFKGA APGFGIAVVA FGAYLAAEKF LFEKKDDHHH

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Macromolecule #35: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 11.039276 KDa
SequenceString:
MSEHQRPELV SFDDINYNDH KKVREAQESY TREQFIRLEA LKTVRKALEK CYEESGPNHF EDCKNLAEQY LDMLPTHRLQ GYLGYQRND PSK

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Macromolecule #36: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 7.807067 KDa
SequenceString:
MAGHHTNYKW NGPLPRPHVS AGHRIATKFL GATMFFWMFL RIKEEYPAML GLHNFYPPKE GEKAVEH

+
Macromolecule #37: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 9.621051 KDa
SequenceString:
MSAALREIRF HLCQNGSSSA PLRQFVKNQI GAFQKANPST KVLVREANGV KPIVFARFDH GHESKIGLDV SSEKEVAERV KSLIEAK

+
Macromolecule #38: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.175141 KDa
SequenceString:
MSSSLYRVLR NAWEVGPRSY WKQLNSIGDT KSGRLVGTDI YGNKFYETDH QDEIHLRTRY VEYKEKDYDM SQVEPGWHFW LGYGVDTAP CNTPKEKLPI RAYPYKFQPN YTGTPGAFVT YNTLKPKISA WEPVTKQRS

+
Macromolecule #39: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.793966 KDa
SequenceString:
MGGGRYPFPK DVISMTGGWW ANPSNWKLNG LFATGIAVGL ALWVSTATLP YTRRREGITS ESDISKWNAA AGVWRERHGK ISTGEAAES E

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Macromolecule #40: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 13.523068 KDa
SequenceString:
FGSHGHGPEL TPAVPFFQPY VLKWAGVSLG LVAFYQFNSS YEAKNGHTWV ETFFHPKSRE DILNEEAKIV QALNNQRELT IKMHELKRE EKDYAHSYSP LFSDPVPQGG SIGKAPGSSR E

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Macromolecule #41: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 11.219027 KDa
SequenceString:
MAEFPPLLSQ EDMKKHKILL AYRDRCAALL VPLNECRKKN YYMPWACGHE RHEYEMCEVA DFQRRVKAMD KLKAEKIEQA KAAAAAAAA AAAADAEESK

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Macromolecule #42: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 10.035651 KDa
SequenceString:
MTAAGISLTG GRNRCFSEWQ SFMHCTAKTD AKSRAQCLPN FEDYMECLHH TKEKARLREI ESVLKQKKEG LEAPPVKVIP VKAIGLVEE

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Macromolecule #43: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 43 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #44: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 44 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Macromolecule #45: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 45 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

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Macromolecule #46: DIUNDECYL PHOSPHATIDYL CHOLINE

MacromoleculeName: DIUNDECYL PHOSPHATIDYL CHOLINE / type: ligand / ID: 46 / Number of copies: 6 / Formula: PLC
Molecular weightTheoretical: 622.834 Da
Chemical component information

ChemComp-PLC:
DIUNDECYL PHOSPHATIDYL CHOLINE / phospholipid*YM

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Macromolecule #47: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 47 / Number of copies: 18 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

+
Macromolecule #48: Lauryl Maltose Neopentyl Glycol

MacromoleculeName: Lauryl Maltose Neopentyl Glycol / type: ligand / ID: 48 / Number of copies: 2 / Formula: LMN
Molecular weightTheoretical: 1.005188 KDa
Chemical component information

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

+
Macromolecule #49: Phosphatidylinositol

MacromoleculeName: Phosphatidylinositol / type: ligand / ID: 49 / Number of copies: 3 / Formula: T7X
Molecular weightTheoretical: 887.128 Da
Chemical component information

ChemComp-T7X:
Phosphatidylinositol

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Macromolecule #50: 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 50 / Number of copies: 1 / Formula: CPL
Molecular weightTheoretical: 758.06 Da
Chemical component information

ChemComp-CPL:
1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

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Macromolecule #51: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 51 / Number of copies: 6 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

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Macromolecule #52: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 52 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

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Macromolecule #53: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 53 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #54: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 54 / Number of copies: 2 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

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Macromolecule #55: water

MacromoleculeName: water / type: ligand / ID: 55 / Number of copies: 1616 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.5 mg/mL
BufferpH: 7.2
Component:
ConcentrationFormulaName
20.0 mMTris
100.0 mMNaClsodium cloride
1.0 mMEDTA
0.025 %LMNG
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 30 eV
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Detector mode: COUNTING / Number real images: 21770 / Average exposure time: 3.0 sec. / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 96899 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: -2.2 µm / Nominal defocus min: -0.8 µm / Nominal magnification: 165000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1078960
CTF correctionSoftware - Name: Gctf
Startup modelType of model: EMDB MAP
EMDB ID:

4873

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 178960
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationNumber classes: 3 / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

6rfr

RefinementSpace: REAL / Protocol: OTHER / Overall B value: 30
Output model

PDB-7o71:
Cryo-EM structure of a respiratory complex I

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