+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-11697 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Human pre-Bact-2 spliceosome | |||||||||
![]() | Unmasked/unsharpened map of the human pre-Bact-2 spliceosome. | |||||||||
![]() |
| |||||||||
Function / homology | ![]() RES complex / microfibril / somatic diversification of immunoglobulins / U11/U12 snRNP / post-mRNA release spliceosomal complex / regulation of retinoic acid receptor signaling pathway / 3'-5' RNA helicase activity / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding ...RES complex / microfibril / somatic diversification of immunoglobulins / U11/U12 snRNP / post-mRNA release spliceosomal complex / regulation of retinoic acid receptor signaling pathway / 3'-5' RNA helicase activity / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / generation of catalytic spliceosome for first transesterification step / U7 snRNP / B-WICH complex / regulation of vitamin D receptor signaling pathway / histone pre-mRNA 3'end processing complex / cis assembly of pre-catalytic spliceosome / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / embryonic brain development / splicing factor binding / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / protein methylation / U12-type spliceosomal complex / miRNA processing / methylosome / nuclear retinoic acid receptor binding / Prp19 complex / 7-methylguanosine cap hypermethylation / positive regulation of androgen receptor activity / poly(A) binding / U1 snRNP binding / mRNA 3'-end processing / blastocyst formation / pICln-Sm protein complex / pre-mRNA binding / U2-type catalytic step 1 spliceosome / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / sno(s)RNA-containing ribonucleoprotein complex / SMN-Sm protein complex / regulation of mRNA splicing, via spliceosome / spliceosomal tri-snRNP complex / P granule / telomerase holoenzyme complex / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / positive regulation by host of viral transcription / U2-type precatalytic spliceosome / commitment complex / positive regulation of vitamin D receptor signaling pathway / Transport of Mature mRNA derived from an Intron-Containing Transcript / telomerase RNA binding / transcription regulator inhibitor activity / U2-type prespliceosome assembly / nuclear vitamin D receptor binding / U2-type catalytic step 2 spliceosome / U4 snRNP / Notch binding / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / U2 snRNP / SAGA complex / positive regulation of mRNA splicing, via spliceosome / RNA Polymerase II Transcription Termination / NOTCH4 Intracellular Domain Regulates Transcription / RHOBTB1 GTPase cycle / positive regulation of transcription by RNA polymerase III / Basigin interactions / U1 snRNP / positive regulation of protein targeting to mitochondrion / ubiquitin-ubiquitin ligase activity / WD40-repeat domain binding / NOTCH3 Intracellular Domain Regulates Transcription / positive regulation of neurogenesis / U2-type prespliceosome / K63-linked polyubiquitin modification-dependent protein binding / cyclosporin A binding / nuclear androgen receptor binding / positive regulation of transcription by RNA polymerase I / precatalytic spliceosome / Notch-HLH transcription pathway / positive regulation of transforming growth factor beta receptor signaling pathway / Formation of paraxial mesoderm / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / SMAD binding / regulation of alternative mRNA splicing, via spliceosome / regulation of RNA splicing / blastocyst development / mRNA 3'-splice site recognition / protein localization to nucleus / spliceosomal tri-snRNP complex assembly / localization / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / retinoic acid receptor signaling pathway / U5 snRNA binding / positive regulation of G1/S transition of mitotic cell cycle / U5 snRNP / transcription-coupled nucleotide-excision repair / positive regulation of viral genome replication Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 8.0 Å | |||||||||
![]() | Townsend C / Kastner B / Leelaram MN / Bertram K / Stark H / Luehrmann R | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. Authors: Cole Townsend / Majety N Leelaram / Dmitry E Agafonov / Olexandr Dybkov / Cindy L Will / Karl Bertram / Henning Urlaub / Berthold Kastner / Holger Stark / Reinhard Lührmann / ![]() Abstract: Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway ...Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway of the latter and the mechanism whereby proteins aid its proper folding. Here, we report the cryo-electron microscopy structures of two human, activated spliceosome precursors (that is, pre-B complexes) at core resolutions of 3.9 and 4.2 angstroms. These structures elucidate the order of the numerous protein exchanges that occur during activation, the mutually exclusive interactions that ensure the correct order of ribonucleoprotein rearrangements needed to form the U2/U6 catalytic RNA, and the stepwise folding pathway of the latter. Structural comparisons with mature B complexes reveal the molecular mechanism whereby a conformational change in the scaffold protein PRP8 facilitates final three-dimensional folding of the U2/U6 catalytic RNA. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 171.3 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 80.2 KB 80.2 KB | Display Display | ![]() |
Images | ![]() | 39.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 248.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 248 KB | Display | |
Data in XML | ![]() | 7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7abiMC ![]() 7aavC ![]() 7abfC ![]() 7abgC ![]() 7abhC C: citing same article ( M: atomic model generated by this map |
---|---|
Similar structure data | |
EM raw data | ![]() Data #1: Motion-corrected micrographs (without dose-weighting) of human pre-Bact spliceosome [micrographs - single frame] Data #2: Motion-corrected micrographs (with dose-weighting) of human pre-Bact spliceosome [micrographs - single frame]) |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Unmasked/unsharpened map of the human pre-Bact-2 spliceosome. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.16 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
+Entire : Human pre-Bact-2 spliceosome
+Supramolecule #1: Human pre-Bact-2 spliceosome
+Supramolecule #2: Human pre-Bact-2 spliceosome
+Supramolecule #3: MINX M3 pre-mRNA
+Macromolecule #1: U5 small nuclear ribonucleoprotein 40 kDa protein
+Macromolecule #2: Splicing factor 3B subunit 3
+Macromolecule #3: Small nuclear ribonucleoprotein F
+Macromolecule #4: U2 small nuclear ribonucleoprotein A'
+Macromolecule #5: Splicing factor 3B subunit 4
+Macromolecule #6: Small nuclear ribonucleoprotein G
+Macromolecule #7: Intron-binding protein aquarius
+Macromolecule #8: Splicing factor 3B subunit 5
+Macromolecule #10: U2 small nuclear ribonucleoprotein B''
+Macromolecule #11: Splicing factor 3B subunit 6
+Macromolecule #12: Ubiquitin-like protein 5
+Macromolecule #13: U5 small nuclear ribonucleoprotein 200 kDa helicase
+Macromolecule #14: SNW domain-containing protein 1
+Macromolecule #15: BUD13 homolog
+Macromolecule #16: Smad nuclear-interacting protein 1
+Macromolecule #17: Protein BUD31 homolog
+Macromolecule #18: RNA-binding motif protein, X-linked 2
+Macromolecule #19: Cell division cycle 5-like protein
+Macromolecule #20: Zinc finger matrin-type protein 2
+Macromolecule #21: Beta-catenin-like protein 1
+Macromolecule #22: 116 kDa U5 small nuclear ribonucleoprotein component
+Macromolecule #23: Spliceosome-associated protein CWC15 homolog
+Macromolecule #24: Serine/arginine repetitive matrix protein 1
+Macromolecule #25: DNA/RNA-binding protein KIN17
+Macromolecule #26: Pre-mRNA-splicing factor SYF1
+Macromolecule #27: Microfibrillar-associated protein 1
+Macromolecule #28: Crooked neck-like protein 1
+Macromolecule #29: PHD finger-like domain-containing protein 5A
+Macromolecule #31: Pleiotropic regulator 1
+Macromolecule #32: Small nuclear ribonucleoprotein Sm D2
+Macromolecule #33: Peptidyl-prolyl cis-trans isomerase E
+Macromolecule #34: RING-type E3 ubiquitin-protein ligase PPIL2
+Macromolecule #35: Small nuclear ribonucleoprotein-associated proteins B and B'
+Macromolecule #37: Small nuclear ribonucleoprotein Sm D3
+Macromolecule #38: Pre-mRNA-processing-splicing factor 8
+Macromolecule #39: Pre-mRNA-processing factor 17
+Macromolecule #40: Small nuclear ribonucleoprotein E
+Macromolecule #41: Pre-mRNA-splicing factor 38A
+Macromolecule #42: Small nuclear ribonucleoprotein Sm D1
+Macromolecule #43: Pre-mRNA-splicing factor RBM22
+Macromolecule #45: Splicing factor 3A subunit 1
+Macromolecule #46: Splicing factor 3A subunit 2
+Macromolecule #47: Splicing factor 3A subunit 3
+Macromolecule #48: Splicing factor 3B subunit 1
+Macromolecule #49: Splicing factor 3B subunit 2
+Macromolecule #9: U6 snRNA
+Macromolecule #30: U2 snRNA
+Macromolecule #36: MINX M3 pre-mRNA
+Macromolecule #44: U5 snRNA
+Macromolecule #50: GUANOSINE-5'-TRIPHOSPHATE
+Macromolecule #51: MAGNESIUM ION
+Macromolecule #52: INOSITOL HEXAKISPHOSPHATE
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.9 |
---|---|
Grid | Model: Quantifoil R3.5/1 / Material: COPPER |
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average exposure time: 1.0 sec. / Average electron dose: 2.27 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: OTHER / Details: cryoSPARC ab initio |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 8.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 39336 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: RIGID BODY FIT |
---|---|
Output model | ![]() PDB-7abi: |