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- PDB-6vbp: Crystal structure of anti-HIV-1 antibody DH815 bound to gp120 V2 ... -

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Basic information

Entry
Database: PDB / ID: 6vbp
TitleCrystal structure of anti-HIV-1 antibody DH815 bound to gp120 V2 peptide
Components
  • DH815 heavy chain
  • DH815 light chain
  • Envelope glycoprotein gp160
KeywordsIMMUNE SYSTEM/Viral Protein / Antibody / GP120 V2 / RV305/RV144 / IMMUNE SYSTEM / IMMUNE SYSTEM-Viral Protein complex
Function / homology
Function and homology information


positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / positive regulation of establishment of T cell polarity / virus-mediated perturbation of host defense response / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / viral protein processing / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope ...positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / positive regulation of establishment of T cell polarity / virus-mediated perturbation of host defense response / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / viral protein processing / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / plasma membrane
Similarity search - Function
Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120
Similarity search - Domain/homology
Envelope glycoprotein gp160
Similarity search - Component
Biological speciesHomo sapiens (human)
Human immunodeficiency virus 1
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.298 Å
AuthorsJanus, B.M. / Ofek, G.
CitationJournal: JCI Insight / Year: 2020
Title: HIV vaccine delayed boosting increases Env variable region 2-specific antibody effector functions.
Authors: Easterhoff, D. / Pollara, J. / Luo, K. / Janus, B. / Gohain, N. / Williams, L.D. / Tay, M.Z. / Monroe, A. / Peachman, K. / Choe, M. / Min, S. / Lusso, P. / Zhang, P. / Go, E.P. / Desaire, H. ...Authors: Easterhoff, D. / Pollara, J. / Luo, K. / Janus, B. / Gohain, N. / Williams, L.D. / Tay, M.Z. / Monroe, A. / Peachman, K. / Choe, M. / Min, S. / Lusso, P. / Zhang, P. / Go, E.P. / Desaire, H. / Bonsignori, M. / Hwang, K.K. / Beck, C. / Kakalis, M. / O'Connell, R.J. / Vasan, S. / Kim, J.H. / Michael, N.L. / Excler, J.L. / Robb, M.L. / Rerks-Ngarm, S. / Kaewkungwal, J. / Pitisuttithum, P. / Nitayaphan, S. / Sinangil, F. / Tartaglia, J. / Phogat, S. / Wiehe, K. / Saunders, K.O. / Montefiori, D.C. / Tomaras, G.D. / Moody, M.A. / Arthos, J. / Rao, M. / Joyce, M.G. / Ofek, G.A. / Ferrari, G. / Haynes, B.F.
History
DepositionDec 19, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 12, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
L: DH815 light chain
H: DH815 heavy chain
B: DH815 light chain
A: DH815 heavy chain
D: DH815 light chain
C: DH815 heavy chain
F: Envelope glycoprotein gp160
E: Envelope glycoprotein gp160
G: Envelope glycoprotein gp160
I: DH815 light chain
J: DH815 heavy chain
K: Envelope glycoprotein gp160
hetero molecules


Theoretical massNumber of molelcules
Total (without water)202,94213
Polymers202,90712
Non-polymers351
Water9,422523
1
L: DH815 light chain
H: DH815 heavy chain
G: Envelope glycoprotein gp160


Theoretical massNumber of molelcules
Total (without water)50,7273
Polymers50,7273
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4940 Å2
ΔGint-36 kcal/mol
Surface area20060 Å2
MethodPISA
2
B: DH815 light chain
A: DH815 heavy chain
F: Envelope glycoprotein gp160


Theoretical massNumber of molelcules
Total (without water)50,7273
Polymers50,7273
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4960 Å2
ΔGint-34 kcal/mol
Surface area20320 Å2
MethodPISA
3
D: DH815 light chain
C: DH815 heavy chain
E: Envelope glycoprotein gp160
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,7624
Polymers50,7273
Non-polymers351
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5180 Å2
ΔGint-45 kcal/mol
Surface area20540 Å2
MethodPISA
4
I: DH815 light chain
J: DH815 heavy chain
K: Envelope glycoprotein gp160


Theoretical massNumber of molelcules
Total (without water)50,7273
Polymers50,7273
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4870 Å2
ΔGint-31 kcal/mol
Surface area19970 Å2
MethodPISA
Unit cell
Length a, b, c (Å)69.903, 158.531, 92.646
Angle α, β, γ (deg.)90.000, 110.270, 90.000
Int Tables number4
Space group name H-MP1211

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Components

#1: Antibody
DH815 light chain


Mass: 24082.684 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#2: Protein
DH815 heavy chain


Mass: 23968.834 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#3: Protein/peptide
Envelope glycoprotein gp160 / Env polyprotein


Mass: 2675.172 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Human immunodeficiency virus 1 / References: UniProt: I2E6B7
#4: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 523 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.37 Å3/Da / Density % sol: 48.17 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 4.2
Details: 0.2M sodium chloride, 20% PEG 8000 and 0.1M sodium citrate pH 4.2.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: RAYONIX MX300-HS / Detector: CCD / Date: Aug 2, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.298→50 Å / Num. obs: 80681 / % possible obs: 95.9 % / Redundancy: 3 % / Rpim(I) all: 0.075 / Net I/σ(I): 9.31
Reflection shellResolution: 2.298→2.35 Å / Redundancy: 2.8 % / Num. unique obs: 6592 / Rpim(I) all: 0.483 / % possible all: 77.8

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Processing

Software
NameVersionClassification
PHENIX1.14_3228refinement
PDB_EXTRACT3.25data extraction
HKL-2000data reduction
HKL-2000data scaling
PHENIX1.14_3228phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4KMT

4kmt


Resolution: 2.298→45.152 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 1.38 / Phase error: 24.68 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2287 1983 2.46 %
Rwork0.1803 78616 -
obs0.1815 80599 95.96 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 189.06 Å2 / Biso mean: 54.6552 Å2 / Biso min: 17.61 Å2
Refinement stepCycle: final / Resolution: 2.298→45.152 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13641 0 1 523 14165
Biso mean--59.08 44.81 -
Num. residues----1790
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.2982-2.35570.30541000.2699409870
2.3557-2.41940.32361400.2599562096
2.4194-2.49060.31851420.2544565897
2.4906-2.5710.31161420.2483565597
2.571-2.66280.32821420.2382566497
2.6628-2.76940.2851460.2317564397
2.7694-2.89550.25191370.2253570497
2.8955-3.04810.2881520.2162569897
3.0481-3.2390.24621390.1936571698
3.239-3.4890.2061520.1772579199
3.489-3.840.20761470.164583299
3.84-4.39520.17421490.1413581299
4.3952-5.53590.16471410.1289581899
5.5359-45.1520.23091540.1657590799
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.0987-0.81553.05881.8745-0.58687.5458-0.4576-0.14680.15710.14020.02130.4231-0.9173-0.4140.34650.3523-0.0061-0.02550.332-0.02820.3375-22.661530.9177-53.7779
22.3712-0.01491.35192.13941.5554.75870.135-0.1977-0.09250.105-0.12370.17460.2616-0.31490.03060.3124-0.04550.01230.4706-0.00980.2798-29.40122.227-57.9238
32.51350.92111.79580.88120.96592.4530.1386-0.11060.0194-0.0081-0.14310.0450.2347-0.2102-0.0170.34480.014-0.01270.3283-0.02790.2434-19.401726.5862-55.3421
42.53990.7110.57792.5414-0.19333.1457-0.13510.084-0.315-0.14810.1179-1.0525-0.38840.9023-0.12730.3311-0.1070.12330.4965-0.13320.586516.484539.6675-57.5927
54.35691.9203-3.36562.8426-0.85113.8288-0.08790.51710.4504-0.33190.23430.28580.2338-0.0219-0.25810.3129-0.0367-0.02150.36140.02610.25272.210941.5198-52.1381
60.52280.6736-0.49822.36120.1570.7867-0.03040.33150.1063-0.20410.3962-0.1804-0.304-0.2156-0.31060.4475-0.0070.00130.47920.07430.36743.873847.4186-59.1432
72.57541.3503-1.42523.08730.043.57760.28730.16960.3698-0.04540.00940.0298-0.33690.1879-0.27030.34110.00810.02680.31850.0360.35056.433342.9353-52.2788
84.38342.13-0.82233.44540.12571.8686-0.0168-0.0197-0.1746-0.0187-0.0288-0.1430.07170.35740.08190.3396-0.0076-0.08070.46790.00820.2087-15.416615.7884-75.1453
92.8273-0.5372-0.41620.10950.29433.7694-0.14540.26130.0694-0.26180.01860.1912-0.13360.09480.13020.37930.0446-0.11710.4746-0.01420.3164-22.155718.7911-82.943
102.9961.3601-1.22541.47120.51583.16580.0851-0.0946-0.13120.0241-0.0829-0.11740.17220.0672-0.07660.29420.0432-0.07270.47280.00850.3538-16.721916.8831-74.812
112.77211.61720.32592.57131.05132.0531-0.10230.3452-0.26810.05010.1996-0.27570.00090.1109-0.01660.25980.0342-0.00250.4102-0.03920.30558.855828.8551-60.0056
123.38371.28111.66392.04150.94987.1430.24650.3742-0.54250.25670.2819-0.73060.25581.0323-0.57250.35470.0846-0.08950.5278-0.06130.549818.063524.869-57.2354
135.8623-0.371-3.21462.2365-0.26822.721-0.4392-0.0757-0.98620.1805-0.11710.16870.3480.15120.60880.39170.04390.05270.3008-0.02050.4546-42.5207-21.5518-46.023
146.84220.1174-0.92652.79571.49971.3943-0.1451-0.1179-0.0254-0.2096-0.1460.3310.0529-0.20990.25980.30620.02530.03210.323-0.02950.4486-50.883-12.9362-45.6604
154.337-0.2663-1.01830.89480.98733.4501-0.01280.67680.2517-0.0727-0.24310.2249-0.1182-0.43640.27290.28960.06110.01550.3127-0.05740.5038-48.8767-12.4179-52.6875
161.6464-1.293-0.57831.4320.64690.2266-0.11820.2193-0.01140.1221-0.02990.2262-0.01470.04610.14570.36250.00810.06880.3517-0.02250.3947-40.343-16.6868-44.6564
172.52260.3380.71443.61121.40044.84140.0226-0.3361-0.07840.45520.5973-0.8597-0.41061.2031-0.40410.4039-0.0201-0.140.5518-0.07310.4463-10.1453-24.4545-21.8652
182.8036-0.96162.42890.3938-0.93315.32580.22080.0597-0.32070.05870.21060.1439-0.12260.0783-0.41760.3123-0.02690.01390.3003-0.02040.343-20.3839-29.4422-31.8665
192.44280.6982.09341.8499-1.53994.72820.7165-0.0647-0.5050.0551-0.32580.15280.13910.221-0.41690.35440.01690.04680.3368-0.03060.311-22.6307-32.6106-23.3597
200.9128-0.78662.03113.1959-1.41954.5430.0009-0.1380.02580.28430.115-0.2452-0.0647-0.0198-0.11380.26690.01910.00960.3529-0.02780.3148-16.6747-30.3576-29.4341
212.3621-0.60971.84252.854-0.15742.12880.05690.2572-0.12150.2008-0.12810.19290.13770.29030.0860.42330.07480.1080.3382-0.01260.318-45.5304-0.2024-29.7301
223.2829-0.54520.13961.4960.99544.11150.0741-0.2159-0.40120.5128-0.19880.47960.0356-0.17830.11220.41140.01040.10750.3969-0.05450.4964-55.323-1.2783-25.4944
233.2082-1.5370.86811.67950.51041.8223-0.0472-0.0257-0.13680.13120.07940.0736-0.1081-0.0119-0.07680.3533-0.01460.08070.2469-0.02460.4554-46.6171-1.2718-30.3818
242.0777-1.18080.60643.0629-0.1781.4084-0.18580.09580.01070.15090.1067-0.1981-0.35420.13140.08090.4057-0.0632-0.02340.2814-0.02520.2331-18.61-13.6357-26.0492
251.57770.78561.20672.3129-0.36041.53330.17870.16060.1116-0.4069-0.0754-0.6833-1.04910.4824-0.08550.5103-0.17430.06950.439-0.02790.4137-10.1082-13.2606-30.2939
266.1933-4.4477-1.95413.42322.21373.3471-0.54760.02340.27030.88540.7346-0.6903-0.4430.8898-0.34090.6738-0.1067-0.02810.6025-0.18380.7519-6.6873-11.3578-23.9798
272.1729-0.19892.36862.5958-0.65526.084-0.22140.0750.11570.1039-0.0030.0302-0.478-0.32170.22070.31330.05710.05680.3296-0.03270.285-0.661826.2818-21.1556
282.7244-0.72771.87683.4945-1.33423.6865-0.27790.2160.36960.5237-0.2061-0.6098-0.71060.75910.28870.2778-0.0894-0.04350.3266-0.00730.363912.758120.8274-14.2078
292.6203-1.05920.81711.3043-0.68681.81230.15860.2035-0.1694-0.2470.00680.03980.15190.1243-0.07080.28510.0184-0.02670.2752-0.02070.25272.276116.668-23.2162
301.7351-0.82230.62360.9481-0.80920.594-0.0005-0.09580.17990.08810.0083-0.13720.022-0.0921-0.00910.28620.0280.00080.2336-0.0410.2227-4.280323.3663-19.3448
312.8995-0.64620.00672.3196-1.03613.0904-0.1810.1169-0.56780.13490.28850.2820.1406-0.6215-0.13140.3002-0.01430.01870.35680.04360.3641-40.592129.1237-17.3786
324.5184-0.7439-3.87761.36760.99994.5780.1119-0.21360.3530.19160.0121-0.0386-0.00430.3221-0.14940.2933-0.0063-0.00770.3424-0.00710.3353-27.462635.2699-17.611
335.6977-0.9766-4.37311.72860.43194.60810.0950.04610.33260.14950.02060.13760.0191-0.0363-0.08540.2966-0.0342-0.00650.2981-0.00740.3266-31.467635.0822-20.1728
344.7571-0.6994-0.82511.8985-0.47723.1794-0.0498-0.1486-0.56630.01490.06040.13940.3198-0.2003-0.00870.3221-0.0401-0.06520.24550.02740.304-5.17694.4807-5.4761
352.2972-0.63280.50791.7734-1.48993.35810.0184-0.066-0.0489-0.0470.08480.2010.068-0.1002-0.08130.2736-0.00370.0030.29120.01420.3019-34.566219.0922-20.5644
363.0961-1.0171-0.35858.1185-1.64954.1871-0.06761.2755-0.3905-0.3090.07370.9298-0.0191-0.2139-0.07950.38580.0678-0.07950.704-0.01450.4925-61.8152.2857-41.6642
373.430.38910.27026.86561.58572.5220.03230.0628-0.2456-0.4842-0.2506-0.60930.30320.42430.28690.57320.1364-0.08760.45560.03230.389212.77951.3793-9.2523
385.1342-0.1033-0.21846.7143-0.63141.67380.0358-0.1322-0.4344-0.4977-0.55380.7340.1574-0.4250.36230.4568-0.1393-0.05510.5943-0.11270.3662-35.21111.326-74.114
395.1977-0.357-0.79151.612-0.40171.8014-0.03630.01320.10380.03870.06950.0927-0.0768-0.0724-0.03940.2388-0.00080.05490.29830.01720.326-37.345459.5595-24.6253
403.8999-1.8219-1.21251.10690.73251.62910.01490.17580.13430.03130.01670.0609-0.0548-0.1438-0.04990.26950.01640.07020.2640.02070.3601-29.037357.2641-21.6365
411.8098-0.9181.31143.8664-1.07263.53080.1677-0.0264-0.1020.45310.1883-0.12720.12190.0931-0.26630.27010.02520.03030.3427-0.08330.2836-4.767843.1646-8.523
423.6256-1.12221.3072.3648-1.58251.17760.13080.4727-0.8792-0.57640.36180.7120.6838-0.0502-0.04950.5171-0.0103-0.01780.427-0.12580.3578-10.482436.1271-6.8228
431.5014-0.61.26353.3269-1.30094.09150.08250.00480.07960.32090.0846-0.2570.23070.3082-0.17530.33010.0015-0.01270.3826-0.07670.3514-4.417941.0379-10.9016
444.1853-0.85142.27562.4407-0.37152.3986-0.4728-0.61640.39730.49620.0824-0.4656-0.9849-0.13910.18320.61230.0630.02570.4904-0.12090.5647-29.595266.08692.7278
453.7242-0.47372.09631.998-1.19343.1655-0.3251-0.73150.25030.50760.3420.3933-0.6013-0.7143-0.02520.52510.14420.13190.5681-0.05980.5552-41.425165.9068-2.8669
463.163-0.5151.46290.740.07913.2914-0.271-0.43160.38710.24680.177-0.0931-0.6096-0.31910.03990.41780.07570.06870.4356-0.12150.5-37.153565.9711-3.5992
474.2851-2.08882.73691.9435-0.91741.9429-0.3182-0.9173-0.32720.64070.38820.3051-0.5324-0.53830.05420.56770.02990.09210.605-0.07310.4607-20.845656.26766.5734
483.2803-0.57730.57592.9789-1.16515.91730.23170.02970.0886-0.0978-0.3716-0.56340.4770.8533-0.14530.37930.11550.04470.5077-0.04440.38842.951949.2763-3.8637
492.7145-1.62930.09571.08730.31634.2320.2350.09090.31040.14480.0191-0.3519-0.6325-0.1636-0.11630.4297-0.03690.04630.3231-0.05610.4192-7.748355.588-2.952
502.9439-1.84241.162.71751.17723.11350.33230.48450.0087-0.0977-0.3167-1.0292-0.89360.8169-0.10320.3609-0.05080.0150.63820.11060.54183.221155.4189-8.5834
512.14750.86220.84212.4038-0.44453.9988-0.35010.28290.75940.64010.3837-0.7227-0.12410.2708-0.0350.506-0.0685-0.06240.4243-0.11670.5397-0.506458.36422.6726
521.5949-0.49391.22965.5289-0.65622.94960.2888-0.04050.34060.13640.05770.1148-1.136-0.6545-0.39060.62520.24260.19230.80910.00890.8715-51.542872.796-12.9881
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'L' and (resid 1 through 18 )L1 - 18
2X-RAY DIFFRACTION2chain 'L' and (resid 19 through 81 )L19 - 81
3X-RAY DIFFRACTION3chain 'L' and (resid 82 through 119 )L82 - 119
4X-RAY DIFFRACTION4chain 'L' and (resid 120 through 134 )L120 - 134
5X-RAY DIFFRACTION5chain 'L' and (resid 135 through 156 )L135 - 156
6X-RAY DIFFRACTION6chain 'L' and (resid 157 through 169 )L157 - 169
7X-RAY DIFFRACTION7chain 'L' and (resid 170 through 212 )L170 - 212
8X-RAY DIFFRACTION8chain 'H' and (resid 1 through 52 )H1 - 52
9X-RAY DIFFRACTION9chain 'H' and (resid 53 through 73 )H53 - 73
10X-RAY DIFFRACTION10chain 'H' and (resid 74 through 111 )H74 - 111
11X-RAY DIFFRACTION11chain 'H' and (resid 112 through 193 )H112 - 193
12X-RAY DIFFRACTION12chain 'H' and (resid 194 through 214 )H194 - 214
13X-RAY DIFFRACTION13chain 'B' and (resid 1 through 18 )B1 - 18
14X-RAY DIFFRACTION14chain 'B' and (resid 19 through 54 )B19 - 54
15X-RAY DIFFRACTION15chain 'B' and (resid 55 through 81 )B55 - 81
16X-RAY DIFFRACTION16chain 'B' and (resid 82 through 119 )B82 - 119
17X-RAY DIFFRACTION17chain 'B' and (resid 120 through 134 )B120 - 134
18X-RAY DIFFRACTION18chain 'B' and (resid 135 through 156 )B135 - 156
19X-RAY DIFFRACTION19chain 'B' and (resid 157 through 169 )B157 - 169
20X-RAY DIFFRACTION20chain 'B' and (resid 170 through 213 )B170 - 213
21X-RAY DIFFRACTION21chain 'A' and (resid 1 through 52 )A1 - 52
22X-RAY DIFFRACTION22chain 'A' and (resid 53 through 73 )A53 - 73
23X-RAY DIFFRACTION23chain 'A' and (resid 74 through 111 )A74 - 111
24X-RAY DIFFRACTION24chain 'A' and (resid 112 through 180 )A112 - 180
25X-RAY DIFFRACTION25chain 'A' and (resid 181 through 208 )A181 - 208
26X-RAY DIFFRACTION26chain 'A' and (resid 209 through 214 )A209 - 214
27X-RAY DIFFRACTION27chain 'D' and (resid 1 through 18 )D1 - 18
28X-RAY DIFFRACTION28chain 'D' and (resid 19 through 38 )D19 - 38
29X-RAY DIFFRACTION29chain 'D' and (resid 39 through 96 )D39 - 96
30X-RAY DIFFRACTION30chain 'D' and (resid 97 through 119 )D97 - 119
31X-RAY DIFFRACTION31chain 'D' and (resid 120 through 134 )D120 - 134
32X-RAY DIFFRACTION32chain 'D' and (resid 135 through 169 )D135 - 169
33X-RAY DIFFRACTION33chain 'D' and (resid 170 through 213 )D170 - 213
34X-RAY DIFFRACTION34chain 'C' and (resid 1 through 111 )C1 - 111
35X-RAY DIFFRACTION35chain 'C' and (resid 112 through 216 )C112 - 216
36X-RAY DIFFRACTION36chain 'F' and (resid 172 through 184)F172 - 184
37X-RAY DIFFRACTION37chain 'E' and (resid 172 through 184 )E172 - 184
38X-RAY DIFFRACTION38chain 'G' and (resid 171 through 184 )G171 - 184
39X-RAY DIFFRACTION39chain 'I' and (resid 1 through 81 )I1 - 81
40X-RAY DIFFRACTION40chain 'I' and (resid 82 through 119 )I82 - 119
41X-RAY DIFFRACTION41chain 'I' and (resid 120 through 156 )I120 - 156
42X-RAY DIFFRACTION42chain 'I' and (resid 157 through 169 )I157 - 169
43X-RAY DIFFRACTION43chain 'I' and (resid 170 through 213 )I170 - 213
44X-RAY DIFFRACTION44chain 'J' and (resid 1 through 17 )J1 - 17
45X-RAY DIFFRACTION45chain 'J' and (resid 18 through 73 )J18 - 73
46X-RAY DIFFRACTION46chain 'J' and (resid 74 through 111 )J74 - 111
47X-RAY DIFFRACTION47chain 'J' and (resid 112 through 124 )J112 - 124
48X-RAY DIFFRACTION48chain 'J' and (resid 125 through 140 )J125 - 140
49X-RAY DIFFRACTION49chain 'J' and (resid 141 through 180 )J141 - 180
50X-RAY DIFFRACTION50chain 'J' and (resid 181 through 199 )J181 - 199
51X-RAY DIFFRACTION51chain 'J' and (resid 200 through 215 )J200 - 215
52X-RAY DIFFRACTION52chain 'K' and (resid 172 through 183 )K172 - 183

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