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- PDB-6hei: Structure of the catalytic domain of USP28 (insertion deleted) bo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6hei | |||||||||
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Title | Structure of the catalytic domain of USP28 (insertion deleted) bound to Ubiquitin-PA | |||||||||
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Function / homology | ![]() protein deubiquitination involved in ubiquitin-dependent protein catabolic process / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Gersch, M. / Komander, D. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Distinct USP25 and USP28 Oligomerization States Regulate Deubiquitinating Activity. Authors: Gersch, M. / Wagstaff, J.L. / Toms, A.V. / Graves, B. / Freund, S.M.V. / Komander, D. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.9 KB | Display | ![]() |
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PDB format | ![]() | 79.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6hehC ![]() 6hejC ![]() 6hekC ![]() 6helC ![]() 6hemC ![]() 1nbfS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 44586.691 Da / Num. of mol.: 1 Mutation: residues 400-579 replaced by GSGSGS,residues 400-579 replaced by GSGSGS,residues 400-579 replaced by GSGSGS,residues 400-579 replaced by GSGSGS Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 8713.023 Da / Num. of mol.: 1 / Mutation: residue 76 replaced with PA warhead Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
#3: Chemical | ChemComp-EDO / ![]() |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.5 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 22% (w/v) PEG 3350, 300 mM potassium sodium tartrate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 9, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.64→49.51 Å / Num. obs: 52145 / % possible obs: 99.8 % / Redundancy: 4 % / Biso Wilson estimate: 24 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.049 / Rrim(I) all: 0.057 / Net I/σ(I): 13.5 |
Reflection shell | Resolution: 1.64→1.7 Å / Redundancy: 4 % / Rmerge(I) obs: 0.637 / Mean I/σ(I) obs: 2.3 / Num. unique obs: 5114 / CC1/2: 0.69 / Rrim(I) all: 0.733 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1NBF Resolution: 1.64→49.51 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 20.71
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.64→49.51 Å
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Refine LS restraints |
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LS refinement shell |
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