+Open data
-Basic information
Entry | Database: PDB / ID: 5yi4 | ||||||||||||||||||
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Title | Solution Structure of the DISC1/Ndel1 complex | ||||||||||||||||||
Components | Disrupted in schizophrenia 1 homolog,Nuclear distribution protein nudE-like 1 | ||||||||||||||||||
Keywords | PROTEIN BINDING / DISC1 / Ndel1 / coiled coil / psychiatric disorder | ||||||||||||||||||
Function / homology | Function and homology information neurofilament cytoskeleton / pyramidal neuron migration to cerebral cortex / cerebral cortex radially oriented cell migration / establishment of chromosome localization / central nervous system neuron axonogenesis / radial glia-guided pyramidal neuron migration / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / mitochondrial calcium ion homeostasis / oligopeptidase activity / central region of growth cone ...neurofilament cytoskeleton / pyramidal neuron migration to cerebral cortex / cerebral cortex radially oriented cell migration / establishment of chromosome localization / central nervous system neuron axonogenesis / radial glia-guided pyramidal neuron migration / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / mitochondrial calcium ion homeostasis / oligopeptidase activity / central region of growth cone / cell proliferation in forebrain / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / regulation of dendritic spine development / RHO GTPases Activate Formins / nuclear membrane disassembly / Separation of Sister Chromatids / regulation of synapse maturation / vesicle transport along microtubule / neurofilament cytoskeleton organization / microtubule nucleation / lysosome localization / dynein complex / axon hillock / positive regulation of ubiquitin-dependent protein catabolic process / non-motile cilium assembly / activation of GTPase activity / retrograde axonal transport / positive regulation of ruffle assembly / mitotic centrosome separation / protein localization to centrosome / microtubule associated complex / intermediate filament cytoskeleton / centrosome localization / positive regulation of cell-matrix adhesion / microtubule organizing center / inner cell mass cell proliferation / regulation of postsynapse organization / regulation of neuron projection development / ciliary base / neuron projection extension / kinesin complex / positive regulation of neuroblast proliferation / beta-tubulin binding / positive regulation of axon regeneration / regulation of intracellular protein transport / cell leading edge / establishment of mitotic spindle orientation / kinesin binding / cilium assembly / alpha-tubulin binding / positive regulation of Wnt signaling pathway / TOR signaling / GABA-ergic synapse / canonical Wnt signaling pathway / positive regulation of axon extension / response to electrical stimulus / axon cytoplasm / regulation of synaptic transmission, glutamatergic / ciliary basal body / negative regulation of protein binding / chromosome segregation / neuron migration / protein localization / kinetochore / positive regulation of GTPase activity / microtubule cytoskeleton organization / positive regulation of neuron projection development / neuron cellular homeostasis / spindle / neuron projection development / cell migration / synaptic vesicle / presynapse / insulin receptor signaling pathway / nuclear envelope / nervous system development / cell body / ubiquitin-dependent protein catabolic process / microtubule binding / microtubule / postsynaptic density / molecular adaptor activity / axon / centrosome / glutamatergic synapse / protein-containing complex binding / perinuclear region of cytoplasm / endoplasmic reticulum / mitochondrion / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||||||||||||||
Biological species | Mus musculus (house mouse) | ||||||||||||||||||
Method | SOLUTION NMR / simulated annealing | ||||||||||||||||||
Authors | Ye, F. / Yu, C. / Yu, C. / Zhang, M. | ||||||||||||||||||
Funding support | Hong Kong, China, 5items
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Citation | Journal: Neuron / Year: 2017 Title: DISC1 Regulates Neurogenesis via Modulating Kinetochore Attachment of Ndel1/Nde1 during Mitosis. Authors: Ye, F. / Kang, E. / Yu, C. / Qian, X. / Jacob, F. / Yu, C. / Mao, M. / Poon, R.Y.C. / Kim, J. / Song, H. / Ming, G.L. / Zhang, M. | ||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5yi4.cif.gz | 850.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5yi4.ent.gz | 718 KB | Display | PDB format |
PDBx/mmJSON format | 5yi4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yi/5yi4 ftp://data.pdbj.org/pub/pdb/validation_reports/yi/5yi4 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15455.417 Da / Num. of mol.: 1 / Fragment: UNP residues 765-852,UNP residues 238-284 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Disc1, Ndel1, Nudel Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: Q811T9, UniProt: Q9ERR1 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 50 NaCl mM / Ionic strength err: 0.2 / Label: conditions_1 / pH: 6.5 / Pressure: 1 Pa / Pressure err: 0.01 / Temperature: 303 K / Temperature err: 0.2 |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |