PDB-5xh6: Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RVR varia...

ID or keywords:

Entry

Database: PDB / ID: 5xh6

Title

Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RVR variant in complex with crRNA and target DNA (TATA PAM)

Components

CRISPR-associated endonuclease Cpf1
Non-target DNA strand
Target DNA strand
crRNA

Keywords

HYDROLASE/RNA/DNA /

nuclease / HYDROLASE-RNA-DNA complex

Function / homology

Function and homology information

Bacillus subtilis ribonuclease /

Bacillus subtilis ribonuclease activity /

deoxyribonuclease I /

deoxyribonuclease I activity / defense response to virus /

lyase activity /

DNA binding /

Biological species

Acidaminococcus sp.

Acidaminococcus sp. BV3L6 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2 Å

Authors

Nishimasu, H. / Yamano, T. / Ishitani, R. / Nureki, O.

Citation

Journal: Mol. Cell / Year: 2017
Title: Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1
Authors: Nishimasu, H. / Yamano, T. / Gao, L. / Zhang, F. / Ishitani, R. / Nureki, O.

History

Deposition	Apr 19, 2017	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Jun 14, 2017	Provider: repository / Type: Initial release
Revision 1.1	Jul 26, 2017	Group: Database references / Category: citation Item: _citation.journal_abbrev / _citation.journal_id_ISSN ..._citation.journal_abbrev / _citation.journal_id_ISSN / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed
Revision 1.2	Nov 22, 2023	Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/xh/5xh6 ftp://data.pdbj.org/pub/pdb/validation_reports/xh/5xh6	HTTPS FTP

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Related structure data

Related structure data	5xh7C 5b43S C: citing same article (ref.) S: Starting model for refinement
Similar structure data	5b43-d 5xh7-d 6ehm-d 5xuu-d 6gtg-d 5ttk-d 5kk5-d 6i1l-2 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#181 - Jan 2015 Cascade and CRISPR similarity (2) #253 - Jan 2021 Expressome similarity (2) #121 - Jan 2010 70S Ribosomes similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (1) #67 - Jul 2005 TATA-Binding Protein similarity (1) #157 - Jan 2013 Transfer-Messenger RNA similarity (1) #260 - Aug 2021 Ribonuclease P similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #229 - Jan 2019 Fluorescent RNA Aptamers similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #87 - Mar 2007 Zinc Fingers similarity (2) #255 - Mar 2021 Cisplatin and DNA similarity (1) #249 - Sep 2020 SARS-CoV-2 RNA-dependent RNA Polymerase similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (1) #15 - Mar 2001 Transfer RNA similarity (1) #227 - Nov 2018 Telomerase similarity (2) #10 - Oct 2000 Ribosome similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #84 - Dec 2006 Transposase similarity (1) #210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #135 - Mar 2011 Integrase similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #245 - May 2020 Spliceosomes similarity (1) #105 - Sep 2008 Ribonuclease A similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #65 - May 2005 Self-splicing RNA similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (1) #98 - Feb 2008 Small Interfering RNA similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #89 - May 2007 Aconitase and Iron Regulatory Protein 1 similarity (1) #266 - Feb 2022 Oligosaccharyltransferase similarity (1) #125 - May 2010 Parvoviruses similarity (1) #131 - Nov 2010 Inteins similarity (1) #230 - Feb 2019 Initiation Factor eIF4E similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #23 - Nov 2001 DNA similarity (1) #161 - May 2013 Ricin similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #81 - Sep 2006 Elongation Factors similarity (1) #143 - Nov 2011 Toll-like Receptors similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #106 - Oct 2008 Poly(A) Polymerase similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #104 - Aug 2008 Selenocysteine Synthase similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (2) #19 - Jul 2001 Actin similarity (1) #18 - Jun 2001 Myosin similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

A: CRISPR-associated endonuclease Cpf1

B: crRNA

C: Target DNA strand

D: Non-target DNA strand

hetero molecules

180 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: gel filtration

Unit cell

Length a, b, c (Å)	81.162, 133.676, 199.633
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P2₁2₁2₁

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DNA chain , 2 types, 2 molecules CD

#3: DNA chain	Target DNA strand Mass: 10434.712 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: DNA (30-MER) / Source: (synth.) Acidaminococcus sp. BV3L6 (bacteria)
#4: DNA chain	Non-target DNA strand Mass: 3003.992 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: DNA (5'-D(CPAPGPTPCPCPTPAPTPA)-3') / Source: (synth.) Acidaminococcus sp. BV3L6 (bacteria)

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Protein / RNA chain , 2 types, 2 molecules AB

#1: Protein	CRISPR-associated endonuclease Cpf1 / AsCpf1 Mass: 151784.391 Da / Num. of mol.: 1 / Mutation: S542R, K548V, N552R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acidaminococcus sp. (strain BV3L6) (bacteria) Strain: BV3L6 / Gene: cpf1, HMPREF1246_0236 / Production host: Escherichia coli (E. coli) References: UniProt: U2UMQ6, Hydrolases; Acting on ester bonds
#2: RNA chain	crRNA Mass: 13756.139 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: RNA (40-MER) / Source: (synth.) Acidaminococcus sp. BV3L6 (bacteria)

#1: Protein

CRISPR-associated endonuclease Cpf1 / AsCpf1

Mass: 151784.391 Da / Num. of mol.: 1 / Mutation: S542R, K548V, N552R
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Acidaminococcus sp. (strain BV3L6) (bacteria)
Strain: BV3L6 / Gene: cpf1, HMPREF1246_0236 / Production host:

Escherichia coli (E. coli)
References: UniProt: U2UMQ6,

Hydrolases; Acting on ester bonds

#2: RNA chain

crRNA

Mass: 13756.139 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: RNA (40-MER) / Source: (synth.)

Acidaminococcus sp. BV3L6 (bacteria)

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Non-polymers , 4 types, 683 molecules

#5: Chemical	ChemComp-NA / SODIUM ION Mass: 22.990 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Na
#6: Chemical	ChemComp-CL / CHLORIDE ION / Chloride Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#7: Chemical	ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol Mass: 62.068 Da / Num. of mol.: 18 / Source method: obtained synthetically / Formula: C₂H₆O₂
#8: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 659 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.12 Å³/Da / Density % sol: 60.55 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 7-10% PEG 3350, 100mM sodium acetate, pH 4.5, 10% 1,6-hexanediol

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å
Detector	Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 18, 2016
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2→39.99 Å / Num. obs: 143997 / % possible obs: 98.2 % / Redundancy: 6.6 % / Net I/σ(I): 17.1
Reflection shell	Resolution: 2→2.0227 Å / CC_1/2: 0.813 / R_pim(I) all: 0.396

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Processing

Software

Name	Version	Classification
PHENIX	(1.11.1_2575: ???)	refinement
DIALS		data reduction
Aimless		data scaling
MOLREP		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 5B43

5b43

Resolution: 2→39.99 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.39

	R_factor	Num. reflection	% reflection
R_free	0.2099	7064	4.91 %
R_work	0.175	-	-
obs	0.1767	143851	97.88 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Refinement step

Cycle: LAST / Resolution: 2→39.99 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	10378	1639	78	659	12754

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.007	12595
X-RAY DIFFRACTION	f_angle_d	0.843	17388
X-RAY DIFFRACTION	f_dihedral_angle_d	17.485	7368
X-RAY DIFFRACTION	f_chiral_restr	0.05	1957
X-RAY DIFFRACTION	f_plane_restr	0.005	1958

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2-2.0227	0.3327	236	0.2724	4535	X-RAY DIFFRACTION	98
2.0227-2.0465	0.3085	246	0.2485	4468	X-RAY DIFFRACTION	98
2.0465-2.0715	0.2838	242	0.2386	4562	X-RAY DIFFRACTION	98
2.0715-2.0977	0.2699	224	0.2337	4516	X-RAY DIFFRACTION	98
2.0977-2.1253	0.2642	246	0.2329	4490	X-RAY DIFFRACTION	98
2.1253-2.1544	0.2861	221	0.2236	4310	X-RAY DIFFRACTION	94
2.1544-2.1852	0.2791	228	0.2154	4286	X-RAY DIFFRACTION	93
2.1852-2.2178	0.2302	260	0.2009	4476	X-RAY DIFFRACTION	98
2.2178-2.2525	0.2474	215	0.1898	4581	X-RAY DIFFRACTION	99
2.2525-2.2894	0.2487	214	0.1922	4565	X-RAY DIFFRACTION	98
2.2894-2.3289	0.2343	256	0.1818	4531	X-RAY DIFFRACTION	99
2.3289-2.3712	0.2241	257	0.1881	4574	X-RAY DIFFRACTION	99
2.3712-2.4168	0.2438	239	0.1833	4544	X-RAY DIFFRACTION	99
2.4168-2.4661	0.2316	249	0.1803	4556	X-RAY DIFFRACTION	99
2.4661-2.5197	0.2268	254	0.1782	4587	X-RAY DIFFRACTION	99
2.5197-2.5784	0.2287	255	0.1798	4537	X-RAY DIFFRACTION	99
2.5784-2.6428	0.2253	231	0.1803	4606	X-RAY DIFFRACTION	99
2.6428-2.7143	0.2374	225	0.1793	4573	X-RAY DIFFRACTION	98
2.7143-2.7941	0.2298	218	0.1885	4314	X-RAY DIFFRACTION	93
2.7941-2.8843	0.2525	215	0.1899	4572	X-RAY DIFFRACTION	98
2.8843-2.9873	0.2067	231	0.1865	4633	X-RAY DIFFRACTION	99
2.9873-3.1069	0.2249	204	0.1837	4650	X-RAY DIFFRACTION	99
3.1069-3.2482	0.2129	258	0.1799	4643	X-RAY DIFFRACTION	99
3.2482-3.4194	0.2358	240	0.1795	4602	X-RAY DIFFRACTION	99
3.4194-3.6335	0.2042	242	0.1734	4648	X-RAY DIFFRACTION	99
3.6335-3.9138	0.1887	226	0.1709	4649	X-RAY DIFFRACTION	99
3.9138-4.3073	0.1953	202	0.1548	4483	X-RAY DIFFRACTION	94
4.3073-4.9296	0.1742	244	0.1453	4716	X-RAY DIFFRACTION	100
4.9296-6.207	0.1898	240	0.1661	4787	X-RAY DIFFRACTION	99
6.207-39.9977	0.1645	246	0.1585	4793	X-RAY DIFFRACTION	96

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.2095	0.4344	-0.357	0.6477	-0.532	1.2789	0.0843	-0.1289	0.1284	0.0481	0.0034	-0.0015	-0.4168	0.0501	-0.1115	0.3584	0.0218	-0.0473	0.2448	0.041	0.2705	34.4714	10.4105	-37.5259
2	2.5993	1.25	-1.3358	1.7068	-1.6698	3.2452	0.0275	0.2335	0.4819	0.1473	0.3203	0.5556	-0.5676	-0.7274	-0.3555	0.3857	0.1333	-0.0309	0.6852	0.0606	0.4667	8.7092	7.5354	-24.6206
3	0.5161	0.0957	0.0171	0.6608	-0.2219	1.3293	0.0789	-0.13	0.0666	0.2058	0.0156	-0.0682	-0.4424	-0.2038	-0.0778	0.3676	0.0072	0.0188	0.3307	-0.01	0.3316	23.2326	5.3658	-7.483
4	1.2731	0.708	-1.1998	1.8004	-1.5367	3.8044	-0.0949	-0.212	0.0926	0.1485	0.0521	-0.1153	-0.3974	0.5236	0.0497	0.3837	0.0216	-0.0458	0.474	-0.1081	0.3866	48.5868	-1.717	16.3963
5	1.1634	0.3047	0.9081	0.7064	0.0114	1.1585	0.0017	-0.1904	0.1829	-0.0547	-0.0485	0.0679	-0.3631	-0.3146	0.0587	0.468	0.0618	0.0205	0.3573	0.0464	0.3677	27.5037	13.4178	-54.583
6	1.3263	-0.297	0.0349	1.0842	-0.2426	1.4053	0.0501	-0.0442	-0.0965	-0.017	0.0354	0.1098	-0.0878	-0.175	-0.0869	0.3408	0.0055	0.0076	0.2582	0.068	0.2958	29.8235	1.2447	-53.7085
7	0.5578	-0.0687	0.2533	0.8403	-0.5422	0.9472	-0.0024	0.0662	0.043	0.0435	-0.0844	-0.0965	0.017	0.304	0.0853	0.2936	0.0138	0.0185	0.3214	0.0189	0.2409	51.2889	-15.3418	-33.9506
8	0.6162	0.2931	-0.2222	0.4004	-0.1894	0.9164	-0.0347	-0.105	0.0216	0.0235	-0.0287	0.0389	0.0577	0.0741	0.0669	0.3207	0.04	-0.009	0.2797	0.0209	0.3201	35.5019	-23.5982	-25.3232
9	1.7861	0.3524	-0.169	1.3259	-1.676	5.5943	0.0141	-0.2639	-0.1579	-0.0344	-0.0483	-0.0006	0.02	0.0968	0.029	0.3245	0.0221	0.0185	0.2075	0.0047	0.3227	31.713	-42.4895	-12.5781
10	2.6284	-1.8113	-1.0065	4.2199	-0.3826	2.3485	-0.0424	-0.2226	-0.0705	-0.0171	-0.033	-0.2778	-0.0694	0.4284	0.0894	0.2515	-0.0279	-0.03	0.3602	0.0659	0.3104	55.5048	-6.5868	-36.0733
11	1.7031	-0.7084	-0.789	1.6668	0.3116	2.1761	0.0446	-0.1273	-0.0591	-0.0751	-0.0333	-0.1845	-0.0528	0.3169	-0.0334	0.2683	-0.0229	-0.0193	0.2872	0.057	0.251	46.5032	-4.5356	-39.008
12	0.6073	-0.0827	1.4512	0.4349	-0.278	3.3351	0.1885	-0.0845	-0.0115	0.1951	-0.0475	-0.0947	-0.1746	-0.0933	-0.1318	0.4074	-0.0445	-0.032	0.4706	0.0056	0.3233	36.6688	-0.993	-7.7465
13	5.4387	3.7978	-2.047	6.9217	2.1717	8.2816	0.2704	0.0729	-0.2805	0.935	-0.3703	-0.5494	0.4222	0.6407	0.0913	0.4541	0.0347	-0.0577	0.5884	0.0806	0.4521	33.2322	-10.1242	10.7383
14	9.7346	-2.6701	1.406	7.3573	2.8315	6.309	-0.3585	0.1158	-0.0456	0.6989	-0.1663	-0.822	0.3502	2.1777	0.5317	0.5146	0.0736	-0.0386	0.9349	0.0821	0.4388	37.023	-10.9206	2.6971
15	0.407	-0.0951	1.5883	0.2198	-1.1676	5.9273	0.0169	0.0115	0.0892	0.0818	0.0216	0.0903	-0.373	-0.1134	-0.0383	0.358	0.0401	0.0061	0.3505	0.0048	0.3195	29.8463	5.0509	-28.5759
16	4.1105	5.2002	2.239	8.2317	0.6834	4.0929	0.2293	0.0568	2.8824	-0.4728	-1.0018	-0.3775	-2.3566	0.6876	0.524	1.2036	-0.0064	0.1075	0.5151	0.1125	1.455	22.9144	33.1815	-49.5823
17	4.3322	-0.7314	1.1961	4.5171	-2.0586	2.6824	-0.0421	0.8519	1.2647	-0.812	-0.005	0.2229	-0.9942	-0.4978	-0.0731	0.973	0.1534	-0.0306	0.4857	0.1436	0.5585	22.5229	24.973	-51.2196

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 1 through 68 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 69 through 152 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 153 through 342 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 343 through 521 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 522 through 646 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 647 through 804 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 805 through 925 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 926 through 1112 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 1113 through 1307 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid -19 through -10 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid -9 through 5 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 6 through 15 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 16 through 20 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid -19 through -15 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid -14 through 5 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 6 through 10 )
17	X-RAY DIFFRACTION	17	chain 'D' and (resid -10 through -1 )

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