+Open data
-Basic information
Entry | Database: PDB / ID: 5nyj | ||||||
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Title | Anbu from Hyphomicrobium sp. strain MC1 - SG: C2 | ||||||
Components | Anbu | ||||||
Keywords | HYDROLASE / Ntn-hydrolase-fold / proteasome / evolution | ||||||
Function / homology | Uncharacterised conserved protein UCP009120, proteasome-type protease, Sll0069 / proteasome core complex / Proteasome subunit / Proteasome, subunit alpha/beta / Nucleophile aminohydrolases, N-terminal / proteolysis involved in protein catabolic process / Peptidase Function and homology information | ||||||
Biological species | Hyphomicrobium sp. | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å | ||||||
Authors | Vielberg, M.-T. / Groll, M. | ||||||
Funding support | Germany, 1items
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Citation | Journal: J. Mol. Biol. / Year: 2018 Title: On the Trails of the Proteasome Fold: Structural and Functional Analysis of the Ancestral beta-Subunit Protein Anbu. Authors: Vielberg, M.T. / Bauer, V.C. / Groll, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5nyj.cif.gz | 707.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5nyj.ent.gz | 595.1 KB | Display | PDB format |
PDBx/mmJSON format | 5nyj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ny/5nyj ftp://data.pdbj.org/pub/pdb/validation_reports/ny/5nyj | HTTPS FTP |
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-Related structure data
Related structure data | 5nyfSC 5nygC 5nypC 5nyqC 5nyrC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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Unit cell |
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-Components
#1: Protein | Mass: 27478.965 Da / Num. of mol.: 16 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Hyphomicrobium sp. (strain MC1) (bacteria) Gene: HYPMC_4374 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: F8JB59 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.55 Å3/Da / Density % sol: 65.35 % |
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Crystal grow | Temperature: 298.15 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.01 M cobalt(II) chloride, 0.1 M Tris, 20 % PVP K15. The protein crystallized in presence of the proteasome inhibitor bortezomib. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 19, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→40 Å / Num. obs: 99540 / % possible obs: 98.8 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.084 / Net I/σ(I): 9.7 |
Reflection shell | Resolution: 3.2→3.3 Å / Rmerge(I) obs: 0.562 / Mean I/σ(I) obs: 2 / % possible all: 98.6 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5NYF Resolution: 3.2→20 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.906 / SU B: 28.845 / SU ML: 0.464 / Cross valid method: THROUGHOUT / ESU R Free: 0.514 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 112.126 Å2
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Refinement step | Cycle: 1 / Resolution: 3.2→20 Å
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Refine LS restraints |
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