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Yorodumi- PDB-5nxa: Crystal structure of Neanderthal Adenylosuccinate Lyase (ADSL)in ... -
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-Basic information
Entry | Database: PDB / ID: 5nxa | |||||||||
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Title | Crystal structure of Neanderthal Adenylosuccinate Lyase (ADSL)in complex with its products AICAR and fumarate | |||||||||
Components | Adenylosuccinate lyase | |||||||||
Keywords | LYASE / Adenylosuccinate Lyase / fumarase | |||||||||
Function / homology | Function and homology information adenylosuccinate lyase / N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity / (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity / 'de novo' AMP biosynthetic process / 'de novo' IMP biosynthetic process Similarity search - Function | |||||||||
Biological species | Homo sapiens neanderthalensis (Neandertal) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | |||||||||
Authors | Van Laer, B. / Kapp, U. / Soler-Lopez, M. / Leonard, G. / Mueller-Dieckmann, C. | |||||||||
Citation | Journal: Sci Rep / Year: 2018 Title: Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Authors: Bart Van Laer / Ulrike Kapp / Montserrat Soler-Lopez / Kaja Moczulska / Svante Pääbo / Gordon Leonard / Christoph Mueller-Dieckmann / Abstract: The availability of genomic data from extinct homini such as Neanderthals has caused a revolution in palaeontology allowing the identification of modern human-specific protein substitutions. ...The availability of genomic data from extinct homini such as Neanderthals has caused a revolution in palaeontology allowing the identification of modern human-specific protein substitutions. Currently, little is known as to how these substitutions alter the proteins on a molecular level. Here, we investigate adenylosuccinate lyase, a conserved enzyme involved in purine metabolism for which several substitutions in the modern human protein (hADSL) have been described to affect intelligence and behaviour. During evolution, modern humans acquired a specific substitution (Ala429Val) in ADSL distinguishing it from the ancestral variant present in Neanderthals (nADSL). We show here that despite this conservative substitution being solvent exposed and located distant from the active site, there is a difference in thermal stability, but not enzymology or ligand binding between nADSL and hADSL. Substitutions near residue 429 which do not profoundly affect enzymology were previously reported to cause neurological symptoms in humans. This study also reveals that ADSL undergoes conformational changes during catalysis which, together with the crystal structure of a hitherto undetermined product bound conformation, explains the molecular origin of disease for several modern human ADSL mutants. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5nxa.cif.gz | 712 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5nxa.ent.gz | 590.1 KB | Display | PDB format |
PDBx/mmJSON format | 5nxa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nx/5nxa ftp://data.pdbj.org/pub/pdb/validation_reports/nx/5nxa | HTTPS FTP |
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-Related structure data
Related structure data | 5nx8SC 5nx9C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
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-Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 0 / Refine code: 0
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