+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 4jc3 | ||||||
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タイトル | 14-3-3 protein interaction with Estrogen Receptor Alpha provides a novel drug target interface | ||||||
要素 |
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キーワード | SIGNALING PROTEIN/Peptide / 14-3-3 (14-3-3タンパク質) / ADAPTER PROTEIN / PROTEIN-PROTEIN INTERACTION (タンパク質間相互作用) / SIGNALING PROTEIN-Peptide complex | ||||||
機能・相同性 | 機能・相同性情報 regulation of epithelial cell apoptotic process / antral ovarian follicle growth / G protein-coupled estrogen receptor activity / regulation of branching involved in prostate gland morphogenesis / RUNX1 regulates transcription of genes involved in WNT signaling / RUNX1 regulates estrogen receptor mediated transcription / regulation of toll-like receptor signaling pathway / regulation of epidermal cell division / prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis / protein kinase C inhibitor activity ...regulation of epithelial cell apoptotic process / antral ovarian follicle growth / G protein-coupled estrogen receptor activity / regulation of branching involved in prostate gland morphogenesis / RUNX1 regulates transcription of genes involved in WNT signaling / RUNX1 regulates estrogen receptor mediated transcription / regulation of toll-like receptor signaling pathway / regulation of epidermal cell division / prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis / protein kinase C inhibitor activity / nuclear estrogen receptor activity / epithelial cell proliferation involved in mammary gland duct elongation / positive regulation of epidermal cell differentiation / keratinocyte development / ケラチン / prostate epithelial cord elongation / epithelial cell development / negative regulation of smooth muscle cell apoptotic process / mammary gland branching involved in pregnancy / uterus development / vagina development / androgen metabolic process / TFIIB-class transcription factor binding / steroid hormone mediated signaling pathway / Regulation of localization of FOXO transcription factors / keratinocyte proliferation / phosphoserine residue binding / Activation of BAD and translocation to mitochondria / negative regulation of keratinocyte proliferation / mammary gland alveolus development / intracellular estrogen receptor signaling pathway / establishment of skin barrier / cellular response to estrogen stimulus / estrogen response element binding / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / : / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / intracellular steroid hormone receptor signaling pathway / negative regulation of canonical NF-kappaB signal transduction / protein kinase A signaling / negative regulation of stem cell proliferation / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / Nuclear signaling by ERBB4 / RNA polymerase II preinitiation complex assembly / RHO GTPases activate PKNs / protein localization to chromatin / TBP-class protein binding / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / steroid binding / protein export from nucleus / negative regulation of innate immune response / nitric-oxide synthase regulator activity / ESR-mediated signaling / protein sequestering activity / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / release of cytochrome c from mitochondria / 14-3-3 protein binding / transcription corepressor binding / positive regulation of protein export from nucleus / negative regulation of miRNA transcription / stem cell proliferation / cellular response to estradiol stimulus / Translocation of SLC2A4 (GLUT4) to the plasma membrane / transcription coregulator binding / 細胞分化 / nuclear estrogen receptor binding / positive regulation of nitric-oxide synthase activity / TP53 Regulates Metabolic Genes / negative regulation of protein kinase activity / SUMOylation of intracellular receptors / ユークロマチン / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / negative regulation of DNA-binding transcription factor activity / transcription coactivator binding / beta-catenin binding / Nuclear Receptor transcription pathway / response to estrogen / male gonad development / Constitutive Signaling by Aberrant PI3K in Cancer / Regulation of RUNX2 expression and activity / nuclear receptor activity / positive regulation of nitric oxide biosynthetic process / intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of DNA-binding transcription factor activity / positive regulation of fibroblast proliferation / Ovarian tumor domain proteases / sequence-specific double-stranded DNA binding / response to estradiol / PIP3 activates AKT signaling / phospholipase C-activating G protein-coupled receptor signaling pathway / ATPase binding / positive regulation of cytosolic calcium ion concentration / regulation of inflammatory response / fibroblast proliferation / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / DNA-binding transcription activator activity, RNA polymerase II-specific / Estrogen-dependent gene expression / transcription regulator complex / Extra-nuclear estrogen signaling 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / 分子置換 / 解像度: 2.05 Å | ||||||
データ登録者 | Bier, D. / Ottmann, C. | ||||||
引用 | ジャーナル: Proc. Natl. Acad. Sci. U.S.A. / 年: 2013 タイトル: Interaction of 14-3-3 proteins with the estrogen receptor alpha F domain provides a drug target interface. 著者: De Vries-van Leeuwen, I.J. / da Costa Pereira, D. / Flach, K.D. / Piersma, S.R. / Haase, C. / Bier, D. / Yalcin, Z. / Michalides, R. / Feenstra, K.A. / Jimenez, C.R. / de Greef, T.F. / ...著者: De Vries-van Leeuwen, I.J. / da Costa Pereira, D. / Flach, K.D. / Piersma, S.R. / Haase, C. / Bier, D. / Yalcin, Z. / Michalides, R. / Feenstra, K.A. / Jimenez, C.R. / de Greef, T.F. / Brunsveld, L. / Ottmann, C. / Zwart, W. / de Boer, A.H. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 4jc3.cif.gz | 59.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb4jc3.ent.gz | 47.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 4jc3.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jc/4jc3 ftp://data.pdbj.org/pub/pdb/validation_reports/jc/4jc3 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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Components on special symmetry positions |
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-要素
#1: タンパク質 | 分子量: 26542.914 Da / 分子数: 1 / 断片: UNP residues 1-231 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: HME1, NM_006142, SFN / プラスミド: PPROEX HTB / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): ROSETTA (DE3) / 参照: UniProt: P31947 | ||
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#2: タンパク質・ペプチド | 分子量: 1158.109 Da / 分子数: 1 / 断片: UNP RESIDUES 585-595 / 由来タイプ: 合成 / 由来: (合成) Homo sapiens (ヒト) / 参照: UniProt: P03372 | ||
#3: 化合物 | #4: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.61 Å3/Da / 溶媒含有率: 52.8 % |
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結晶化 | 温度: 277.15 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.5 詳細: 0.095M HEPES Na, 0.19M calcium chloride, 5% glycerol, 26.6% PEG400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
-データ収集
回折 | 平均測定温度: 100 K | |||||||||||||||||||||||||||||||||||||||||||||
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放射光源 | 由来: 回転陽極 / タイプ: BRUKER AXS MICROSTAR / 波長: 1.54 Å | |||||||||||||||||||||||||||||||||||||||||||||
検出器 | タイプ: MAR scanner 345 mm plate / 検出器: IMAGE PLATE / 日付: 2012年8月20日 | |||||||||||||||||||||||||||||||||||||||||||||
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | |||||||||||||||||||||||||||||||||||||||||||||
放射波長 | 波長: 1.54 Å / 相対比: 1 | |||||||||||||||||||||||||||||||||||||||||||||
反射 | 解像度: 2.05→19.52 Å / Num. all: 18518 / Num. obs: 18505 / % possible obs: 99.78 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Biso Wilson estimate: 19.403 Å2 / Rmerge(I) obs: 0.078 / Net I/σ(I): 24.16 | |||||||||||||||||||||||||||||||||||||||||||||
反射 シェル |
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-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 / 解像度: 2.05→19.52 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.887 / SU B: 3.471 / SU ML: 0.098 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.175 / ESU R Free: 0.168 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK | |||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 14.867 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2.05→19.52 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.05→2.103 Å / Total num. of bins used: 20
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