+Open data
-Basic information
Entry | Database: PDB / ID: 3ul0 | ||||||
---|---|---|---|---|---|---|---|
Title | Mouse importin alpha: mouse CBP80Y8D cNLS complex | ||||||
Components |
| ||||||
Keywords | PROTEIN TRANSPORT/PROTEIN BINDING / Arm repeat / Armadillo repeat / nuclear transport / nuclear localisation signal binding / importin beta binding / PROTEIN TRANSPORT-PROTEIN BINDING complex | ||||||
Function / homology | Function and homology information Formation of the Early Elongation Complex / SLBP independent Processing of Histone Pre-mRNAs / FGFR2 alternative splicing / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / Processing of Intronless Pre-mRNAs / Formation of RNA Pol II elongation complex / RNA polymerase II transcribes snRNA genes / mRNA Capping / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Pre-transcription Events ...Formation of the Early Elongation Complex / SLBP independent Processing of Histone Pre-mRNAs / FGFR2 alternative splicing / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / Processing of Intronless Pre-mRNAs / Formation of RNA Pol II elongation complex / RNA polymerase II transcribes snRNA genes / mRNA Capping / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Pre-transcription Events / mRNA Splicing - Minor Pathway / mRNA 3'-end processing / RNA Polymerase II Transcription Termination / Transport of Mature mRNA derived from an Intron-Containing Transcript / snRNA export from nucleus / nuclear cap binding complex / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / mRNA Splicing - Major Pathway / positive regulation of mRNA 3'-end processing / cap-dependent translational initiation / : / RNA cap binding / alternative mRNA splicing, via spliceosome / Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / regulation of mRNA processing / miRNA-mediated post-transcriptional gene silencing / primary miRNA processing / regulatory ncRNA-mediated post-transcriptional gene silencing / positive regulation of viral life cycle / mRNA 3'-end processing / RNA 7-methylguanosine cap binding / NLS-dependent protein nuclear import complex / postsynapse to nucleus signaling pathway / positive regulation of mRNA splicing, via spliceosome / RNA catabolic process / regulation of translational initiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / spliceosomal complex assembly / 7-methylguanosine mRNA capping / mRNA export from nucleus / host cell / positive regulation of transcription elongation by RNA polymerase II / mRNA transcription by RNA polymerase II / mRNA splicing, via spliceosome / cytoplasmic stress granule / protein import into nucleus / positive regulation of cell growth / DNA-binding transcription factor binding / defense response to virus / postsynaptic density / molecular adaptor activity / ribonucleoprotein complex / mRNA binding / glutamatergic synapse / mitochondrion / nucleoplasm / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Marfori, M. / Forwood, J.K. / Lonhienne, T.G. / Kobe, B. | ||||||
Citation | Journal: Traffic / Year: 2012 Title: Structural Basis of High-Affinity Nuclear Localization Signal Interactions with Importin-alpha Authors: Marfori, M. / Lonhienne, T.G. / Forwood, J.K. / Kobe, B. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3ul0.cif.gz | 193.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3ul0.ent.gz | 153.3 KB | Display | PDB format |
PDBx/mmJSON format | 3ul0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ul/3ul0 ftp://data.pdbj.org/pub/pdb/validation_reports/ul/3ul0 | HTTPS FTP |
---|
-Related structure data
Related structure data | 3ukwC 3ukxC 3ukyC 3ukzC 3ul1C 1pjnS C: citing same article (ref.) S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 55268.473 Da / Num. of mol.: 1 / Fragment: UNP Residues 70-529 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Kpna2 / Production host: Escherichia coli (E. coli) / References: UniProt: P52293 |
---|---|
#2: Protein/peptide | Mass: 2904.127 Da / Num. of mol.: 1 / Fragment: mCBP80 cNLS peptide, residues 1-23 / Mutation: Y8D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Cbp80 / Production host: Escherichia coli (E. coli) / References: UniProt: Q3UYV9 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.03 Å3/Da / Density % sol: 59.46 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.7M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E+ SUPERBRIGHT / Wavelength: 1.5419 Å |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: Nov 16, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5419 Å / Relative weight: 1 |
Reflection | Resolution: 2→40.983 Å / Num. obs: 48448 / % possible obs: 99 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2→2.07 Å / % possible all: 99.9 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1PJN Resolution: 2→40.983 Å / Occupancy max: 1 / Occupancy min: 0.26 / SU ML: 0.26 / Phase error: 21.42 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 36.131 Å2 / ksol: 0.358 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 121.58 Å2 / Biso mean: 36.6675 Å2 / Biso min: 12.62 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→40.983 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 17
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|