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Open data
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Basic information
Entry | Database: PDB / ID: 3zin | ||||||
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Title | Gu_alpha_helicase | ||||||
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![]() | TRANSPORT PROTEIN/HYDROLASE / TRANSPORT PROTEIN-HYDROLASE COMPLEX | ||||||
Function / homology | ![]() B-WICH complex positively regulates rRNA expression / Major pathway of rRNA processing in the nucleolus and cytosol / 7SK snRNA binding / R-loop processing / positive regulation of myeloid dendritic cell cytokine production / Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / NLS-dependent protein nuclear import complex / postsynapse to nucleus signaling pathway ...B-WICH complex positively regulates rRNA expression / Major pathway of rRNA processing in the nucleolus and cytosol / 7SK snRNA binding / R-loop processing / positive regulation of myeloid dendritic cell cytokine production / Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / NLS-dependent protein nuclear import complex / postsynapse to nucleus signaling pathway / miRNA binding / nuclear import signal receptor activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chang, C.-W. / Counago, R.M. / Williams, S.J. / Kobe, B. | ||||||
![]() | ![]() Title: Distinctive Conformation of Minor Site-Specific Nuclear Localization Signals Bound to Importin-Alpha Authors: Chang, C.-W. / Counago, R.M. / Williams, S.J. / Boden, M. / Kobe, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 182.3 KB | Display | ![]() |
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PDB format | ![]() | 144.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3zioC ![]() 3zipC ![]() 3ziqC ![]() 3zirC ![]() 1ialS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 49872.836 Da / Num. of mol.: 1 / Fragment: RESIDUES 72-496 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() | ||
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#2: Protein/peptide | Mass: 1587.782 Da / Num. of mol.: 2 / Fragment: RESIDUES 839-851 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #3: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.27 Å3/Da / Density % sol: 62.38 % / Description: NONE |
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Crystal grow![]() | pH: 6.5 Details: 0.8 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT |
-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Jul 31, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→19.83 Å / Num. obs: 49123 / % possible obs: 99.6 % / Observed criterion σ(I): 2 / Redundancy: 7.1 % / Biso Wilson estimate: 33.84 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 10.3 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 14.55 % / Rmerge(I) obs: 0.46 / Mean I/σ(I) obs: 6.5 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1IAL Resolution: 2→19.83 Å / Cor.coef. Fo:Fc: 0.9521 / Cor.coef. Fo:Fc free: 0.9417 / SU R Cruickshank DPI: 0.115 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.116 / SU Rfree Blow DPI: 0.107 / SU Rfree Cruickshank DPI: 0.107
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Displacement parameters | Biso mean: 40.9 Å2
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Refine analyze | Luzzati coordinate error obs: 0.25 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→19.83 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.05 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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