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Open data
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Basic information
Entry | Database: PDB / ID: 2zow | ||||||
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Title | Crystal Structure of H2O2 treated Cu,Zn-SOD | ||||||
![]() | Superoxide dismutase [Cu-Zn] | ||||||
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Function / homology | ![]() neurofilament cytoskeleton organization / protein phosphatase 2B binding / relaxation of vascular associated smooth muscle / response to superoxide / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ito, S. / Ishii, T. / Sakai, H. / Uchida, K. | ||||||
![]() | ![]() Title: Crystal structures of H2O2-treated Cu,Zn-superoxide dismutase Authors: Ito, S. / Ishii, T. / Sakai, H. / Uchida, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.8 KB | Display | ![]() |
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PDB format | ![]() | 57 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2z7uC ![]() 2z7wC ![]() 1q0eS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15573.337 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: sigma-aldrich chemical company / Source: (natural) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.21 % |
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 6.4 Details: 20% (w/v) PEG 4000, 20% (w/v) iso-propanol, 0.09M MES, 1mM EDTA, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K PH range: 6.4-6.7 |
-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Mar 1, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.45→27.94 Å / Num. all: 61832 / Num. obs: 61462 / % possible obs: 99.8 % / Biso Wilson estimate: 18.1 Å2 |
Reflection shell | Resolution: 1.45→1.5 Å / Mean I/σ(I) obs: 3.3 / Rsym value: 0.33 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ID 1Q0E Resolution: 1.45→27.94 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 1245479.95 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 37.5339 Å2 / ksol: 0.337586 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.45→27.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.45→1.54 Å / Rfactor Rfree error: 0.013 / Total num. of bins used: 6
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Xplor file |
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