+Open data
-Basic information
Entry | Database: PDB / ID: 2j0n | ||||||
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Title | A proteolytically truncated form of Shigella Flexneri IpaD | ||||||
Components | INVASIN IPAD | ||||||
Keywords | CELL INVASION / IPAD / T3SS / PLASMID / VIRULENCE / SHIGELLA FLEXNERI / TYPE III SECRETION | ||||||
Function / homology | Function and homology information effector-mediated activation of programmed cell death in host / extracellular region Similarity search - Function | ||||||
Biological species | Shigella flexneri 2a str. 301 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Johnson, S. / Roversi, P. / Lea, S.M. | ||||||
Citation | Journal: J Biol Chem / Year: 2007 Title: Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD. Authors: Steven Johnson / Pietro Roversi / Marianela Espina / Andrew Olive / Janet E Deane / Susan Birket / Terry Field / William D Picking / Ariel J Blocker / Edouard E Galyov / Wendy L Picking / Susan M Lea / Abstract: Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. ...Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2006 Title: Expression, Limited Proteolysis and Preliminary Crystallographic Analysis of Ipad, a Component of the Shigella Flexneri Type III Secretion System Authors: Johnson, S. / Roversi, P. / Espina, M. / Deane, J.E. / Birket, S. / Picking, W.D. / Blocker, A. / Picking, W.L. / Lea, S.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2j0n.cif.gz | 85.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2j0n.ent.gz | 65.7 KB | Display | PDB format |
PDBx/mmJSON format | 2j0n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j0/2j0n ftp://data.pdbj.org/pub/pdb/validation_reports/j0/2j0n | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.287, 0.04773, 0.95674), Vector: |
-Components
#1: Protein | Mass: 22149.730 Da / Num. of mol.: 2 / Fragment: TRUNCATED FORM, RESIDUES 133-332 Source method: isolated from a genetically manipulated source Details: THE TRUNCATION WAS OBTAINED BY IN-DROP CLEAVAGE BY UNKOWN PROTEASE OF A CONSTRUCT CONSISTING OF RESIDUES 15-332 Source: (gene. exp.) Shigella flexneri 2a str. 301 (bacteria) Plasmid: PET-15B / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P18013 #2: Water | ChemComp-HOH / | Sequence details | THE TRUNCATION | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 43 % Description: THE SEARCH MODEL FOR MOLECULAR REPLACEMENT IS AN IPAD POLYMORPH THAT WE ARE GOING TO DEPOSIT SOON. |
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Crystal grow | pH: 7 Details: 10 % (V/V) PEG 400, 0.1 M NACL, 0.1 M TRIS-HCL, PH7.0, pH 7.00 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 21, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2→42 Å / Num. obs: 24238 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 4 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 7.9 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.42 / Mean I/σ(I) obs: 1.7 / % possible all: 99.8 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.1→15 Å / Isotropic thermal model: TNT BCORREL / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: TNT PROTGEO / Details: REFINED IN BUSTER-TNT BETA 1.9.2
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Solvent computation | Solvent model: BABINET SCALING / Bsol: 175 Å2 / ksol: 0.43 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→15 Å
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