[English] 日本語
Yorodumi- PDB-2i0f: Lumazine synthase RibH1 from Brucella abortus (Gene BruAb1_0785, ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2i0f | ||||||
---|---|---|---|---|---|---|---|
Title | Lumazine synthase RibH1 from Brucella abortus (Gene BruAb1_0785, Swiss-Prot entry Q57DY1) | ||||||
Components | 6,7-dimethyl-8-ribityllumazine synthase 1Lumazine synthase | ||||||
Keywords | TRANSFERASE / lumazine synthase RibH1 | ||||||
Function / homology | Function and homology information 6,7-dimethyl-8-ribityllumazine synthase / 6,7-dimethyl-8-ribityllumazine synthase activity / riboflavin synthase complex / riboflavin biosynthetic process Similarity search - Function | ||||||
Biological species | Brucella abortus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.22 Å | ||||||
Authors | Klinke, S. / Zylberman, V. / Bonomi, H.R. / Haase, I. / Guimaraes, B.G. / Braden, B.C. / Bacher, A. / Fischer, M. / Goldbaum, F.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: Structural and Kinetic Properties of Lumazine Synthase Isoenzymes in the Order Rhizobiales Authors: Klinke, S. / Zylberman, V. / Bonomi, H.R. / Haase, I. / Guimaraes, B.G. / Braden, B.C. / Bacher, A. / Fischer, M. / Goldbaum, F.A. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2i0f.cif.gz | 148.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2i0f.ent.gz | 117.5 KB | Display | PDB format |
PDBx/mmJSON format | 2i0f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i0/2i0f ftp://data.pdbj.org/pub/pdb/validation_reports/i0/2i0f | HTTPS FTP |
---|
-Related structure data
Related structure data | 2f59SC 2o6hC 2obxC S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 16807.744 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Brucella abortus (bacteria) / Gene: ribH1, ribH, ribH-1 / Plasmid: pT7-7 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: Q57DY1, 6,7-dimethyl-8-ribityllumazine synthase #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.46 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.7 Details: 12% PEG 4000, 0.1M HEPES, 0.2M CALCIUM CHLORIDE, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: LNLS / Beamline: D03B-MX1 / Wavelength: 1.438 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Nov 22, 2005 |
Radiation | Monochromator: SI SINGLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.438 Å / Relative weight: 1 |
Reflection | Resolution: 2.22→30 Å / Num. all: 35840 / Num. obs: 33602 / % possible obs: 94.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.7 % / Biso Wilson estimate: 43.06 Å2 / Rmerge(I) obs: 0.041 / Rsym value: 0.041 / Net I/σ(I): 12.3 |
Reflection shell | Resolution: 2.22→2.34 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.282 / Mean I/σ(I) obs: 2.6 / Num. unique all: 5150 / Rsym value: 0.282 / % possible all: 88.7 |
-Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2F59 Resolution: 2.22→30 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
| |||||||||||||||||||||||||
Displacement parameters | Biso mean: 44.27 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.22→30 Å
| |||||||||||||||||||||||||
Refine LS restraints |
|