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Yorodumi- PDB-2o6h: Lumazine synthase RibH1 from Brucella melitensis (Gene BMEI1187, ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2o6h | ||||||
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Title | Lumazine synthase RibH1 from Brucella melitensis (Gene BMEI1187, Swiss-Prot entry Q8YGH2) complexed with inhibitor 5-Nitro-6-(D-Ribitylamino)-2,4(1H,3H) Pyrimidinedione | ||||||
Components | 6,7-dimethyl-8-ribityllumazine synthase 1Lumazine synthase | ||||||
Keywords | TRANSFERASE / lumazine synthase | ||||||
Function / homology | Function and homology information 6,7-dimethyl-8-ribityllumazine synthase / 6,7-dimethyl-8-ribityllumazine synthase activity / riboflavin synthase complex / riboflavin biosynthetic process Similarity search - Function | ||||||
Biological species | Brucella melitensis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Klinke, S. / Zylberman, V. / Bonomi, H.R. / Haase, I. / Guimaraes, B.G. / Braden, B.C. / Bacher, A. / Fischer, M. / Goldbaum, F.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: Structural and Kinetic Properties of Lumazine Synthase Isoenzymes in the Order Rhizobiales Authors: Klinke, S. / Zylberman, V. / Bonomi, H.R. / Haase, I. / Guimaraes, B.G. / Braden, B.C. / Bacher, A. / Fischer, M. / Goldbaum, F.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2o6h.cif.gz | 149 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2o6h.ent.gz | 118.6 KB | Display | PDB format |
PDBx/mmJSON format | 2o6h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/o6/2o6h ftp://data.pdbj.org/pub/pdb/validation_reports/o6/2o6h | HTTPS FTP |
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-Related structure data
Related structure data | 2f59SC 2i0fC 2obxC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 16826.791 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Brucella melitensis (bacteria) / Gene: ribH1 / Plasmid: pET11A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: Q8YGH2, 6,7-dimethyl-8-ribityllumazine synthase #2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-INI / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.87 Å3/Da / Density % sol: 57.12 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 6.3 Details: 22% PEG 400, 0.2M CaCl2, 0.1M MES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X9A / Wavelength: 0.9794 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Apr 26, 2006 |
Radiation | Monochromator: Si double crystal monocromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→48.8 Å / Num. all: 27278 / Num. obs: 22799 / % possible obs: 83.5 % / Redundancy: 3.9 % / Biso Wilson estimate: 54.6 Å2 / Rmerge(I) obs: 0.1 / Rsym value: 0.1 / Net I/σ(I): 5.8 |
Reflection shell | Resolution: 2.7→2.85 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.313 / Mean I/σ(I) obs: 2.2 / Num. unique all: 3174 / Rsym value: 0.313 / % possible all: 82.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2F59 Resolution: 2.7→48.8 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 32.68 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→48.8 Å
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Refine LS restraints |
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