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- PDB-2a0t: NMR structure of the FHA1 domain of Rad53 in complex with a biolo... -
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Basic information
Entry | Database: PDB / ID: 2a0t | ||||||
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Title | NMR structure of the FHA1 domain of Rad53 in complex with a biological relevant phosphopeptide derived from Madt1 | ||||||
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Function / homology | ![]() regulation of mRNA stability involved in response to oxidative stress / deoxyribonucleoside triphosphate biosynthetic process / G2/M DNA damage checkpoint / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / meiotic recombination checkpoint signaling / post-mRNA release spliceosomal complex / nuclear mRNA surveillance / negative regulation of phosphorylation / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks ...regulation of mRNA stability involved in response to oxidative stress / deoxyribonucleoside triphosphate biosynthetic process / G2/M DNA damage checkpoint / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / meiotic recombination checkpoint signaling / post-mRNA release spliceosomal complex / nuclear mRNA surveillance / negative regulation of phosphorylation / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Mahajan, A. / Yuan, C. / Pike, B.L. / Heierhorst, J. / Chang, C.-F. / Tsai, M.-D. | ||||||
![]() | ![]() Title: FHA Domain-Ligand Interactions: Importance of Integrating Chemical and Biological Approaches Authors: Mahajan, A. / Yuan, C. / Pike, B.L. / Heierhorst, J. / Chang, C.-F. / Tsai, M.-D. #1: ![]() Title: Solution structures of two FHA1-phosphothreonine peptide complexes provide insight into the structural basis of the ligand specificity of FHA1 from yeast Rad53 Authors: Yuan, C. / Yongkiettrakul, S. / Byeon, I.-J.L. / Zhou, S. / Tsai, M.-D. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 1005.5 KB | Display | ![]() |
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PDB format | ![]() | 868.9 KB | Display | ![]() |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 17093.490 Da / Num. of mol.: 1 / Fragment: N-terminal FHA domain (FHA1) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: SPK1 or Rad53 / Plasmid: pGEX-4T / Species (production host): Escherichia coli / Production host: ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1246.173 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence occurs naturally in Saccharomyces cerevisiae (yeast) References: UniProt: P34217 |
-Experimental details
-Experiment
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NMR experiment |
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Sample preparation
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Sample conditions | Ionic strength: 10 mM sodium phosphate, 1 mM DTT, and 1 mM EDTA pH: 6.5 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||||||||||||
NMR spectrometer |
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Processing
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Refinement | Method: ![]() Details: The complex structures are generated using a total of 2524 NMR constraints including constraints in this PDB file and constraints of free FHA1 in PDB access code 1K3J | ||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average, lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry, structures with the least restraint violations, structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |