Group: Atomic model / Database references ...Atomic model / Database references / Derived calculations / Non-polymer description / Other / Source and taxonomy / Structure summary / Version format compliance
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA, BA" IN EACH CHAIN ON SHEET RECORDS ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA, BA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 12-STRANDED BARREL THIS IS REPRESENTED BY A 13-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "CA, DA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 10-STRANDED BARREL THIS IS REPRESENTED BY A 11-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
分子量: 26887.346 Da / 分子数: 4 / 変異: YES / 由来タイプ: 組換発現 詳細: THE CHROMOPHORE, GYS, IS PART OF THE PEPTIDE CHAIN BETWEEN RESIDUES 64 AND 68. 由来: (組換発現) AEQUOREA VICTORIA (オワンクラゲ) 組織: CIRCUMORAL RING CANAL / 器官: PHOTOGENIC ORGAN / 発現宿主: ESCHERICHIA COLI M15 (大腸菌) / 参照: UniProt: P42212
ENGINEERED MUTATION IN CHAINS A, B, C, D, GLN 80 TO ARG
配列の詳細
MODRES: 1W7T GLU 222 SIDECHAIN IS SPECIFICALLY DECARBOXYLATED AS A RESULT OF PHOTOTRANSFORMATION. ...MODRES: 1W7T GLU 222 SIDECHAIN IS SPECIFICALLY DECARBOXYLATED AS A RESULT OF PHOTOTRANSFORMATION. OE1, OE2, CD ATOMS ARE NOT PRESENT FOR THIS RESIDUE IN ALL CHAINS
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.2 Å3/Da / 溶媒含有率: 43 %
結晶化
温度: 277 K / pH: 7.8 詳細: CRYSTALS WERE GROWN AT 4C FROM 50 MM MGCL2, 14-17 % PEG3350 AND 50-100 MM TRIS/CL PH 7.8 - 8.6.
解像度: 1.85→119.52 Å / Cor.coef. Fo:Fc: 0.944 / Cor.coef. Fo:Fc free: 0.928 / SU B: 2.758 / SU ML: 0.084 / 交差検証法: THROUGHOUT / ESU R: 0.144 / ESU R Free: 0.134 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.22
4162
5 %
RANDOM
Rwork
0.18
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obs
0.182
79139
97.4 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK