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Yorodumi- PDB-1u32: Crystal structure of a Protein Phosphatase-1: Calcineurin Hybrid ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1u32 | ||||||
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Title | Crystal structure of a Protein Phosphatase-1: Calcineurin Hybrid Bound to Okadaic Acid | ||||||
Components | Serine/threonine protein phosphatase PP1-gamma catalytic subunitSerine/threonine-specific protein kinase | ||||||
Keywords | HYDROLASE | ||||||
Function / homology | Function and homology information PTW/PP1 phosphatase complex / regulation of nucleocytoplasmic transport / protein phosphatase 1 binding / lamin binding / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / microtubule organizing center / myosin phosphatase activity / protein serine/threonine phosphatase activity / glycogen metabolic process ...PTW/PP1 phosphatase complex / regulation of nucleocytoplasmic transport / protein phosphatase 1 binding / lamin binding / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / microtubule organizing center / myosin phosphatase activity / protein serine/threonine phosphatase activity / glycogen metabolic process / protein-serine/threonine phosphatase / entrainment of circadian clock by photoperiod / Triglyceride catabolism / phosphatase activity / phosphoprotein phosphatase activity / cleavage furrow / blastocyst development / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / positive regulation of glial cell proliferation / Resolution of Sister Chromatid Cohesion / protein dephosphorylation / Downregulation of TGF-beta receptor signaling / RHO GTPases Activate Formins / RAF activation / circadian regulation of gene expression / regulation of circadian rhythm / neuron differentiation / kinetochore / Separation of Sister Chromatids / MAPK cascade / Circadian Clock / presynapse / midbody / spermatogenesis / mitochondrial outer membrane / dendritic spine / nuclear speck / cell cycle / cell division / protein domain specific binding / focal adhesion / glutamatergic synapse / protein-containing complex binding / nucleolus / protein kinase binding / protein-containing complex / mitochondrion / RNA binding / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Maynes, J.T. / Perreault, K.R. / Cherney, M.M. / Luu, H.A. / James, M.N.G. / Holmes, C.F.B. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2004 Title: Crystal Structure and Mutagenesis of a Protein Phosphatase-1:Calcineurin Hybrid Elucidate the Role of the {beta}12-{beta}13 Loop in Inhibitor Binding Authors: Maynes, J.T. / Perreault, K.R. / Cherney, M.M. / Luu, H.A. / James, M.N.G. / Holmes, C.F.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1u32.cif.gz | 78.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1u32.ent.gz | 57.3 KB | Display | PDB format |
PDBx/mmJSON format | 1u32.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u3/1u32 ftp://data.pdbj.org/pub/pdb/validation_reports/u3/1u32 | HTTPS FTP |
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-Related structure data
Related structure data | 1jk7S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 33771.910 Da / Num. of mol.: 1 / Fragment: residues 6-298 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PPP1CC / Plasmid: pet / Production host: Escherichia coli (E. coli) References: UniProt: P36873, protein-serine/threonine phosphatase | ||||||
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#2: Chemical | #3: Chemical | ChemComp-OKA / | #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 47 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: lithium sulfate, tris, PEG 400, mercaptoethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jul 22, 2002 / Details: osmic mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→40 Å / Num. all: 21891 / Num. obs: 21304 / % possible obs: 97.3 % / Observed criterion σ(F): 1.9 / Observed criterion σ(I): 2.5 / Redundancy: 5.8 % / Biso Wilson estimate: 15 Å2 / Rmerge(I) obs: 0.076 / Rsym value: 0.057 / Net I/σ(I): 21.1 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 5.7 % / Rmerge(I) obs: 0.369 / Mean I/σ(I) obs: 5.02 / Num. unique all: 2063 / Rsym value: 0.36 / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ID 1JK7 Resolution: 2→36.01 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 1946384.85 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 37.2487 Å2 / ksol: 0.359391 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→36.01 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.13 Å / Rfactor Rfree error: 0.019 / Total num. of bins used: 6
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Xplor file |
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