+Open data
-Basic information
Entry | Database: PDB / ID: 1kpb | ||||||
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Title | PKCI-1-APO | ||||||
Components | HUMAN PROTEIN KINASE C INTERACTING PROTEIN 1 (ZINC PROTEIN) | ||||||
Keywords | PROTEIN KINASE C INTERACTING PROTEIN / PKCI-1 / PKC / SIGNAL TRANSDUCTION / ZINC BINDING PROTEIN | ||||||
Function / homology | Function and homology information purine ribonucleotide catabolic process / Hydrolases; Acting on phosphorus-nitrogen bonds / adenosine 5'-monophosphoramidase activity / deSUMOylase activity / protein desumoylation / histone deacetylase complex / intrinsic apoptotic signaling pathway by p53 class mediator / positive regulation of calcium-mediated signaling / protein kinase C binding / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases ...purine ribonucleotide catabolic process / Hydrolases; Acting on phosphorus-nitrogen bonds / adenosine 5'-monophosphoramidase activity / deSUMOylase activity / protein desumoylation / histone deacetylase complex / intrinsic apoptotic signaling pathway by p53 class mediator / positive regulation of calcium-mediated signaling / protein kinase C binding / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / cytoskeleton / hydrolase activity / nucleotide binding / regulation of DNA-templated transcription / signal transduction / proteolysis / extracellular exosome / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2 Å | ||||||
Authors | Lima, C.D. / Klein, M.G. / Weinstein, I.B. / Hendrickson, W.A. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1996 Title: Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins. Authors: Lima, C.D. / Klein, M.G. / Weinstein, I.B. / Hendrickson, W.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1kpb.cif.gz | 58.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1kpb.ent.gz | 43.3 KB | Display | PDB format |
PDBx/mmJSON format | 1kpb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kp/1kpb ftp://data.pdbj.org/pub/pdb/validation_reports/kp/1kpb | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13692.734 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Description: EXPRESSED AS AN ACETYLATED PROTEIN ON AMINO ACID 2. THIS IS SIMILAR TO THE NATIVE PROTEIN PURIFIED DIRECTLY FROM BOVINE BRAIN. Gene: HPKCI-1 / Plasmid: PHIL-D5 / Gene (production host): HPKCI-1 / Production host: Pichia pastoris (fungus) / References: UniProt: P49773 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53 % | ||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 1.283 |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Oct 19, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.283 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 17662 / % possible obs: 89.7 % / Observed criterion σ(I): 6 / Redundancy: 3.6 % / Rmerge(I) obs: 0.042 |
Reflection | *PLUS Num. measured all: 99583 |
-Processing
Software |
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Refinement | Resolution: 2→10 Å / σ(F): 2
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Displacement parameters | Biso mean: 15.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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