+Open data
-Basic information
Entry | Database: PDB / ID: 1h8k | ||||||
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Title | A-SPECTRIN SH3 DOMAIN A11V, V23L, M25V, V53I, V58L MUTANT | ||||||
Components | SPECTRIN ALPHA CHAIN | ||||||
Keywords | SH3-DOMAIN / CYTOSKELETON / CALMODULIN-BINDING / ACTIN-BINDING | ||||||
Function / homology | Function and homology information actin filament capping / costamere / cortical actin cytoskeleton / cell projection / actin filament binding / cell junction / actin cytoskeleton organization / calmodulin binding / calcium ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | GALLUS GALLUS (chicken) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Vega, M.C. / Serrano, L. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2002 Title: Conformational Strain in the Hydrophobic Core and its Implications for Protein Folding and Design Authors: Ventura, S. / Vega, M.C. / Lacroix, E. / Angrand, I. / Spagnolo, L. / Serrano, L. #1: Journal: Nature / Year: 1992 Title: Crystal Structure of a Src-Homology 3 (SH3) Domain Authors: Musacchio, A. / Noble, M. / Pauptit, R. / Wierenga, R. / Saraste, M. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1h8k.cif.gz | 25.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1h8k.ent.gz | 15.8 KB | Display | PDB format |
PDBx/mmJSON format | 1h8k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h8/1h8k ftp://data.pdbj.org/pub/pdb/validation_reports/h8/1h8k | HTTPS FTP |
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-Related structure data
Related structure data | 1e6gC 1e6hC 1shgS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 7254.271 Da / Num. of mol.: 1 / Fragment: SH3-DOMAIN RESIDUES 965-1025 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) GALLUS GALLUS (chicken) / Plasmid: PBR322 / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / References: UniProt: P07751 |
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#2: Water | ChemComp-HOH / |
Compound details | ENGINEERED MUTATION ALA11VAL, VAL23LEU, MET25VAL, VAL53ILE, VAL58LEU CAN BIND ACTIN BUT SEEM TO ...ENGINEERED |
Sequence details | 1SHG SWS P07751 1 - 964 NOT IN CONSTRUCT 1SHG SWS P07751 1026 - 2477 NOT IN CONSTRUCTT |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.76 Å3/Da |
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Crystal grow | pH: 4.5 Details: PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 0.1M ACETATE/AC. ACETIC, PH=4.5, 90 MM BIS-TRIS PROPANE, 0.9 MM SODIUM AZIDE, pH 4.50 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: MACSCIENCE M18X / Wavelength: 1.5418 |
Detector | Type: SMALL MARRESEARCH IMAGING PLATE / Date: Nov 15, 2000 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→12 Å / Num. obs: 2863 / % possible obs: 88.8 % / Observed criterion σ(I): 2 / Redundancy: 2.3 % / Rmerge(I) obs: 0.11 |
Reflection shell | Resolution: 2.7→2.82 Å / % possible all: 71.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1SHG Resolution: 2.7→10 Å / Cross valid method: THROUGHOUT / σ(F): 2 Details: THE 5 FIRST RESIDUE IN N-TERMINAL WAS NOTSEEN IN THE DENSITY MAP
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Refinement step | Cycle: LAST / Resolution: 2.7→10 Å
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Refine LS restraints |
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Xplor file |
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