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Structure paper

TitleStructure of the two-component S-layer of the archaeon .
Journal, issue, pagesElife, Vol. 13, Year 2024
Publish dateJan 22, 2024
AuthorsLavinia Gambelli / Mathew McLaren / Rebecca Conners / Kelly Sanders / Matthew C Gaines / Lewis Clark / Vicki A M Gold / Daniel Kattnig / Mateusz Sikora / Cyril Hanus / Michail N Isupov / Bertram Daum /
PubMed AbstractSurface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell ...Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo electron tomography, and Alphafold2 predictions to generate an atomic model of the two-component S-layer of . The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesise that jackknife-like conformational changes in SlaA play important roles in S-layer assembly.
External linksElife / PubMed:38251732 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution3.1 - 11.2 Å
Structure data

14635

7zcx

EMDB-14635, PDB-7zcx:
S-layer protein SlaA from Sulfolobus acidocaldarius at pH 4.0
Method: EM (single particle) / Resolution: 3.1 Å

15530

8an2

EMDB-15530, PDB-8an2:
S-layer protein SlaA from Sulfolobus acidocaldarius at pH 10.0
Method: EM (single particle) / Resolution: 3.2 Å

15531

8an3

EMDB-15531, PDB-8an3:
S-layer protein SlaA from Sulfolobus acidocaldarius at pH 7.0
Method: EM (single particle) / Resolution: 3.9 Å

18127

8qox

8qp0

EMDB-18127: S-layer of archaeon Sulfolobus acidocaldarius by subtomogram averaging
PDB-8qox: Two-component assembly of SlaA and SlaB S-layer proteins of Sulfolobus acidocaldarius
PDB-8qp0: A hexamer pore in the S-layer of Sulfolobus acidocaldarius formed by SlaA protein
Method: EM (subtomogram averaging) / Resolution: 11.2 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-YZT:
6-deoxy-6-sulfo-beta-D-glucopyranose

Source
  • sulfolobus acidocaldarius dsm 639 (acidophilic)
  • sulfolobus acidocaldarius (acidophilic)
KeywordsSTRUCTURAL PROTEIN / S-layer / dimer / N-glycosylation / archea

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