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TitleAlgal photosystem I dimer and high-resolution model of PSI-plastocyanin complex.
Journal, issue, pagesNat Plants, Vol. 8, Issue 10, Page 1191-1201, Year 2022
Publish dateOct 13, 2022
AuthorsAndreas Naschberger / Laura Mosebach / Victor Tobiasson / Sebastian Kuhlgert / Martin Scholz / Annemarie Perez-Boerema / Thi Thu Hoai Ho / André Vidal-Meireles / Yuichiro Takahashi / Michael Hippler / Alexey Amunts /
PubMed AbstractPhotosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular ...Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer, comprising 40 protein subunits with 118 transmembrane helices that provide scaffold for 568 pigments. Cryogenic electron microscopy identified that the absence of PsaH and Lhca2 gives rise to a head-to-head relative orientation of the PSI-light-harvesting complex I monomers in a way that is essentially different from the oligomer formation in cyanobacteria. The light-harvesting protein Lhca9 is the key element for mediating this dimerization. The interface between the monomers is lacking PsaH and thus partially overlaps with the surface area that would bind one of the light-harvesting complex II complexes in state transitions. We also define the most accurate available PSI-light-harvesting complex I model at 2.3 Å resolution, including a flexibly bound electron donor plastocyanin, and assign correct identities and orientations to all the pigments, as well as 621 water molecules that affect energy transfer pathways.
External linksNat Plants / PubMed:36229605 / PubMed Central
MethodsEM (single particle)
Resolution2.31 - 2.97 Å
Structure data

14867

7zq9

EMDB-14867, PDB-7zq9:
Dimeric PSI of Chlamydomonas reinhardtii at 2.74 A resolution (symmetry expanded)
Method: EM (single particle) / Resolution: 2.74 Å

14870

7zqc

EMDB-14870, PDB-7zqc:
Monomeric PSI of Chlamydomonas reinhardtii at 2.31 A resolution
Method: EM (single particle) / Resolution: 2.31 Å

14871

7zqd

EMDB-14871, PDB-7zqd:
Dimeric PSI of Chlamydomonas reinhardtii at 2.97 A resolution
Method: EM (single particle) / Resolution: 2.97 Å

14872

7zqe

EMDB-14872, PDB-7zqe:
Plastocyanin bound to PSI of Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 2.55 Å

Chemicals

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-HOH:
WATER / Water

ChemComp-CU:
COPPER (II) ION / Copper

Source
  • chlamydomonas reinhardtii (plant)
KeywordsPHOTOSYNTHESIS / green algae / dimeric PSI

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