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-Structure paper
タイトル | Algal photosystem I dimer and high-resolution model of PSI-plastocyanin complex. |
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ジャーナル・号・ページ | Nat Plants, Vol. 8, Issue 10, Page 1191-1201, Year 2022 |
掲載日 | 2022年10月13日 |
著者 | Andreas Naschberger / Laura Mosebach / Victor Tobiasson / Sebastian Kuhlgert / Martin Scholz / Annemarie Perez-Boerema / Thi Thu Hoai Ho / André Vidal-Meireles / Yuichiro Takahashi / Michael Hippler / Alexey Amunts / |
PubMed 要旨 | Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular ...Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer, comprising 40 protein subunits with 118 transmembrane helices that provide scaffold for 568 pigments. Cryogenic electron microscopy identified that the absence of PsaH and Lhca2 gives rise to a head-to-head relative orientation of the PSI-light-harvesting complex I monomers in a way that is essentially different from the oligomer formation in cyanobacteria. The light-harvesting protein Lhca9 is the key element for mediating this dimerization. The interface between the monomers is lacking PsaH and thus partially overlaps with the surface area that would bind one of the light-harvesting complex II complexes in state transitions. We also define the most accurate available PSI-light-harvesting complex I model at 2.3 Å resolution, including a flexibly bound electron donor plastocyanin, and assign correct identities and orientations to all the pigments, as well as 621 water molecules that affect energy transfer pathways. |
リンク | Nat Plants / PubMed:36229605 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.31 - 2.97 Å |
構造データ | EMDB-14867, PDB-7zq9: EMDB-14870, PDB-7zqc: EMDB-14871, PDB-7zqd: EMDB-14872, PDB-7zqe: |
化合物 | ChemComp-CL0: ChemComp-CLA: ChemComp-PQN: ChemComp-LHG: ChemComp-BCR: ChemComp-SF4: ChemComp-LMU: ChemComp-LMG: ChemComp-LUT: ChemComp-DGD: ChemComp-CHL: ChemComp-XAT: ChemComp-NEX: ChemComp-HOH: ChemComp-CU: |
由来 |
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キーワード | PHOTOSYNTHESIS (光合成) / green algae (緑藻) / dimeric PSI |