EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of a FtsZ single protofilament and a double-helical tube in complex with a monobody.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4073, Year 2023
掲載日	2023年7月10日
著者	Junso Fujita / Hiroshi Amesaka / Takuya Yoshizawa / Kota Hibino / Natsuki Kamimura / Natsuko Kuroda / Takamoto Konishi / Yuki Kato / Mizuho Hara / Tsuyoshi Inoue / Keiichi Namba / Shun-Ichi Tanaka / Hiroyoshi Matsumura /
PubMed 要旨	FtsZ polymerizes into protofilaments to form the Z-ring that acts as a scaffold for accessory proteins during cell division. Structures of FtsZ have been previously solved, but detailed mechanistic ...FtsZ polymerizes into protofilaments to form the Z-ring that acts as a scaffold for accessory proteins during cell division. Structures of FtsZ have been previously solved, but detailed mechanistic insights are lacking. Here, we determine the cryoEM structure of a single protofilament of FtsZ from Klebsiella pneumoniae (KpFtsZ) in a polymerization-preferred conformation. We also develop a monobody (Mb) that binds to KpFtsZ and FtsZ from Escherichia coli without affecting their GTPase activity. Crystal structures of the FtsZ-Mb complexes reveal the Mb binding mode, while addition of Mb in vivo inhibits cell division. A cryoEM structure of a double-helical tube of KpFtsZ-Mb at 2.7 Å resolution shows two parallel protofilaments. Our present study highlights the physiological roles of the conformational changes of FtsZ in treadmilling that regulate cell division.
リンク	Nat Commun / PubMed:37429870 / PubMed Central
手法	EM (らせん対称) / X線回折
解像度	1.84 - 3.03 Å
構造データ	34429 8h1o EMDB-34429, PDB-8h1o: Cryo-EM structure of KpFtsZ-monobody double helical tube 手法: EM (らせん対称) / 解像度: 2.67 Å 35344 8ibn EMDB-35344, PDB-8ibn: Cryo-EM structure of KpFtsZ single filament 手法: EM (らせん対称) / 解像度: 3.03 Å PDB-8gzv: Klebsiella pneumoniae FtsZ complexed with monobody (P212121) 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å PDB-8gzw: Klebsiella pneumoniae FtsZ complexed with monobody (P21) 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-8gzx: Escherichia coli FtsZ complexed with monobody (P212121) 手法: X-RAY DIFFRACTION / 解像度: 1.84 Å PDB-8gzy: Escherichia coli FtsZ complexed with monobody (P21) 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-HOH: WATER / 水 ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-K: Unknown entry / カリウムカチオン
由来	klebsiella pneumoniae (肺炎桿菌) homo sapiens (ヒト) escherichia coli (大腸菌)
キーワード	CELL CYCLE (細胞周期) / Cell division (細胞分裂) / Monobody / GTPase (GTPアーゼ) / HYDROLASE (加水分解酵素) / bacterial cell division / divisome / FtsZ (FtsZ) / tubulin (チューブリン)