EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Vaccine elicitation and structural basis for antibody protection against alphaviruses.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 12, Page 2672-2689.e25, Year 2023
掲載日	2023年6月8日
著者	Matthew S Sutton / Sergei Pletnev / Victoria Callahan / Sungyoul Ko / Yaroslav Tsybovsky / Tatsiana Bylund / Ryan G Casner / Gabriele Cerutti / Christina L Gardner / Veronica Guirguis / Raffaello Verardi / Baoshan Zhang / David Ambrozak / Margaret Beddall / Hong Lei / Eun Sung Yang / Tracy Liu / Amy R Henry / Reda Rawi / Arne Schön / Chaim A Schramm / Chen-Hsiang Shen / Wei Shi / Tyler Stephens / Yongping Yang / Maria Burgos Florez / Julie E Ledgerwood / Crystal W Burke / Lawrence Shapiro / Julie M Fox / Peter D Kwong / Mario Roederer /
PubMed 要旨	Alphaviruses are RNA viruses that represent emerging public health threats. To identify protective antibodies, we immunized macaques with a mixture of western, eastern, and Venezuelan equine ...Alphaviruses are RNA viruses that represent emerging public health threats. To identify protective antibodies, we immunized macaques with a mixture of western, eastern, and Venezuelan equine encephalitis virus-like particles (VLPs), a regimen that protects against aerosol challenge with all three viruses. Single- and triple-virus-specific antibodies were isolated, and we identified 21 unique binding groups. Cryo-EM structures revealed that broad VLP binding inversely correlated with sequence and conformational variability. One triple-specific antibody, SKT05, bound proximal to the fusion peptide and neutralized all three Env-pseudotyped encephalitic alphaviruses by using different symmetry elements for recognition across VLPs. Neutralization in other assays (e.g., chimeric Sindbis virus) yielded variable results. SKT05 bound backbone atoms of sequence-diverse residues, enabling broad recognition despite sequence variability; accordingly, SKT05 protected mice against Venezuelan equine encephalitis virus, chikungunya virus, and Ross River virus challenges. Thus, a single vaccine-elicited antibody can protect in vivo against a broad range of alphaviruses.
リンク	Cell / PubMed:37295404 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 7.0 Å
構造データ	27389 8dec EMDB-27389, PDB-8dec: Cryo-EM Structure of Western Equine Encephalitis Virus 手法: EM (単粒子) / 解像度: 4.7 Å 27390 8ded EMDB-27390, PDB-8ded: Cryo-EM Structure of Western Equine Encephalitis Virus VLP in complex with SKW19 fab 手法: EM (単粒子) / 解像度: 4.1 Å 27391 8dee EMDB-27391, PDB-8dee: Asymmetric Unit of Western Equine Encephalitis Virus 手法: EM (単粒子) / 解像度: 3.4 Å 27392 8def EMDB-27392, PDB-8def: Cryo-EM Structure of Western Equine Encephalitis Virus VLP in complex with SKW24 fab 手法: EM (単粒子) / 解像度: 4.2 Å 27395 8deq EMDB-27395, PDB-8deq: Cryo-EM local refinement of antibody SKV09 in complex with VEEV alphavirus spike glycoprotein 手法: EM (単粒子) / 解像度: 6.0 Å 27396 8der EMDB-27396, PDB-8der: Cryo-EM local refinement of antibody SKV16 in complex with VEEV alphavirus spike glycoprotein 手法: EM (単粒子) / 解像度: 3.3 Å 27722 8dul EMDB-27722, PDB-8dul: Cryo-EM Structure of Antibody SKT05 in complex with Western Equine Encephalitis Virus spike (local refinement from VLP particles) 手法: EM (単粒子) / 解像度: 4.17 Å 27723 8dun EMDB-27723, PDB-8dun: Cryo-EM Structure of Antibody SKW11 in complex with Western Equine Encephalitis Virus spike (local refinement from VLP particles) 手法: EM (単粒子) / 解像度: 5.84 Å 27757 8dwo EMDB-27757, PDB-8dwo: Cryo-EM Structure of Eastern Equine Encephalitis Virus in complex with SKE26 Fab 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-28056: Venezuelan equine encephalitis virus-like particle in complex with Fab SKT05 手法: EM (単粒子) / 解像度: 4.1 Å 28058 8eeu EMDB-28058: Venezuelan equine encephalitis virus-like particle in complex with Fab SKT05, local refinement PDB-8eeu: Venezuelan equine encephalitis virus-like particle in complex with Fab SKT05 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-28059: Venezuelan equine encephalitis virus-like particle in complex with Fab SKT20 手法: EM (単粒子) / 解像度: 4.7 Å 28060 8eev EMDB-28060: Venezuelan equine encephalitis virus-like particle in complex with Fab SKT-20, local refinement PDB-8eev: Venezuelan equine encephalitis virus-like particle in complex with Fab SKT-20 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-28115: Western Equine Encephalitis Virus-Like Particle in Complex with SKW19 Fab 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-28116: Western Equine Encephalitis Virus-Like Particle in Complex with SKW24 Fab 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-28117: Eastern Equine Encephalitis Virus-Like Particle in Complex with SKE26 Fab 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-28118: Cryo-EM structure of Antibody SKW11 in complex with Western Equine Encephalitis Virus-Like Particle 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-28119: Cryo-EM structure of Antibody SKT05 in complex with Western Equine Encephalitis Virus-like Particle 手法: EM (単粒子) / 解像度: 5.7 Å EMDB-28187: Cryo-EM I1 reconstruction of antibody SKV09 in complex with VEEV alphavirus VLP 手法: EM (単粒子) / 解像度: 7.0 Å EMDB-28188: Cryo-EM I1 reconstruction of antibody SKV16 in complex with VEEV alphavirus VLP 手法: EM (単粒子) / 解像度: 5.8 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	western equine encephalitis virus (西部ウマ脳炎ウイルス) homo sapiens (ヒト) venezuelan equine encephalitis virus (ベネズエラウマ脳炎ウイルス) macaca (マカク属) macaca fascicularis (カニクイザル) eastern equine encephalitis virus (東部ウマ脳炎ウイルス)
キーワード	VIRUS LIKE PARTICLE (ウイルス様粒子) / Western Equine Encephalitis Virus / Virus-Like Particle (ウイルス様粒子) / VIRUS/IMMUNE SYSTEM / antibody (抗体) / VLP-Fab complex / VIRUS-IMMUNE SYSTEM complex / VIRAL PROTEIN (ウイルスタンパク質) / antibody alphavirus VEEV spike trimer glycoprotein / IMMUNE SYSTEM (免疫系) / SKT05 / broadly neutralizing / SKW11 / Eastern Equine Encephalitis virus / virus-fab complex / VIRUS LIKE PARTICLE/IMMUNE SYSTEM / Venezuelan equine encephalitis virus / neutralizing antibody (中和抗体) / VEEV / VIRUS LIKE PARTICLE-IMMUNE SYSTEM complex