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- EMDB-28059: Venezuelan equine encephalitis virus-like particle in complex wit... -

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Basic information

Entry
Database: EMDB / ID: EMD-28059
TitleVenezuelan equine encephalitis virus-like particle in complex with Fab SKT20
Map dataFinal map after post-processing
Sample
  • Complex: Complex between Venezuelan equine encephalitis virus-like particle and Fab SKT20
    • Protein or peptide: Coat protein
    • Protein or peptide: Fab SKT20 heavy chain
    • Protein or peptide: Fab SKT20 light chain
KeywordsVenezuelan equine encephalitis virus / neutralizing antibody / VEEV / VIRUS LIKE PARTICLE-IMMUNE SYSTEM complex
Biological speciesVenezuelan equine encephalitis virus / Macaca fascicularis (crab-eating macaque)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.7 Å
AuthorsTsybovsky Y / Pletnev S / Verardi R / Roederer M / Kwong PD
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Cell / Year: 2023
Title: Vaccine elicitation and structural basis for antibody protection against alphaviruses.
Authors: Matthew S Sutton / Sergei Pletnev / Victoria Callahan / Sungyoul Ko / Yaroslav Tsybovsky / Tatsiana Bylund / Ryan G Casner / Gabriele Cerutti / Christina L Gardner / Veronica Guirguis / ...Authors: Matthew S Sutton / Sergei Pletnev / Victoria Callahan / Sungyoul Ko / Yaroslav Tsybovsky / Tatsiana Bylund / Ryan G Casner / Gabriele Cerutti / Christina L Gardner / Veronica Guirguis / Raffaello Verardi / Baoshan Zhang / David Ambrozak / Margaret Beddall / Hong Lei / Eun Sung Yang / Tracy Liu / Amy R Henry / Reda Rawi / Arne Schön / Chaim A Schramm / Chen-Hsiang Shen / Wei Shi / Tyler Stephens / Yongping Yang / Maria Burgos Florez / Julie E Ledgerwood / Crystal W Burke / Lawrence Shapiro / Julie M Fox / Peter D Kwong / Mario Roederer /
Abstract: Alphaviruses are RNA viruses that represent emerging public health threats. To identify protective antibodies, we immunized macaques with a mixture of western, eastern, and Venezuelan equine ...Alphaviruses are RNA viruses that represent emerging public health threats. To identify protective antibodies, we immunized macaques with a mixture of western, eastern, and Venezuelan equine encephalitis virus-like particles (VLPs), a regimen that protects against aerosol challenge with all three viruses. Single- and triple-virus-specific antibodies were isolated, and we identified 21 unique binding groups. Cryo-EM structures revealed that broad VLP binding inversely correlated with sequence and conformational variability. One triple-specific antibody, SKT05, bound proximal to the fusion peptide and neutralized all three Env-pseudotyped encephalitic alphaviruses by using different symmetry elements for recognition across VLPs. Neutralization in other assays (e.g., chimeric Sindbis virus) yielded variable results. SKT05 bound backbone atoms of sequence-diverse residues, enabling broad recognition despite sequence variability; accordingly, SKT05 protected mice against Venezuelan equine encephalitis virus, chikungunya virus, and Ross River virus challenges. Thus, a single vaccine-elicited antibody can protect in vivo against a broad range of alphaviruses.
History
DepositionSep 7, 2022-
Header (metadata) releaseJul 19, 2023-
Map releaseJul 19, 2023-
UpdateJul 19, 2023-
Current statusJul 19, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_28059.png

Downloads & links

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Map

FileDownload / File: emd_28059.map.gz / Format: CCP4 / Size: 1.9 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFinal map after post-processing
Voxel sizeX=Y=Z: 1.2315 Å
Density
Contour LevelBy AUTHOR: 0.006
Minimum - Maximum-0.011164391 - 0.02445997
Average (Standard dev.)0.00039993753 (±0.00158596)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions800800800
Spacing800800800
CellA=B=C: 985.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_28059_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Map before post-processing

Fileemd_28059_additional_1.map
AnnotationMap before post-processing
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map 2

Fileemd_28059_half_map_1.map
AnnotationHalf-map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map 1

Fileemd_28059_half_map_2.map
AnnotationHalf-map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Complex between Venezuelan equine encephalitis virus-like particl...

EntireName: Complex between Venezuelan equine encephalitis virus-like particle and Fab SKT20
Components
  • Complex: Complex between Venezuelan equine encephalitis virus-like particle and Fab SKT20
    • Protein or peptide: Coat protein
    • Protein or peptide: Fab SKT20 heavy chain
    • Protein or peptide: Fab SKT20 light chain

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Supramolecule #1: Complex between Venezuelan equine encephalitis virus-like particl...

SupramoleculeName: Complex between Venezuelan equine encephalitis virus-like particle and Fab SKT20
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Venezuelan equine encephalitis virus
Molecular weightTheoretical: 33 MDa

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Macromolecule #1: Coat protein

MacromoleculeName: Coat protein / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Venezuelan equine encephalitis virus
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MFPFQPMYPM QPMPYRNPFA APRRPWFPRT DPFLAMQVQE LTRSMANLTF KQRRDAPPEG PSAKKPKKE ASQKQKGGGQ GKKKKNQGKK KAKTGPPNPK AQNGNKKKTN KKPGKRQRMV MKLESDKTF PIMLEGKING YACVVGGKLF RPMHVEGKID NDVLAALKTK ...String:
MFPFQPMYPM QPMPYRNPFA APRRPWFPRT DPFLAMQVQE LTRSMANLTF KQRRDAPPEG PSAKKPKKE ASQKQKGGGQ GKKKKNQGKK KAKTGPPNPK AQNGNKKKTN KKPGKRQRMV MKLESDKTF PIMLEGKING YACVVGGKLF RPMHVEGKID NDVLAALKTK KASKYDLEYA DV PQNMRAD TFKYTHEKPQ GYYSWHHGAV QYENGRFTVP KGVGAKGDSG RPILDNQGRV VAI VLGGVN EGSRTALSVV MWNEKGVTVK YTPENCEQW SLVTTMCLLA NVTFPCAQPP ICYDR KPAE TLAMLSVNVD NPGYDELLEA AVKCPGRKRR STEELFNEY KLTRPYMARC IRCAVGS CH SPIAIEAVKS DGHDGYVRLQ TSSQYGLDSS GNLKGRTMRY DMHGTIKEIP LHQVSLYT S RPCHIVDGHG YFLLARCPAG DSITMEFKKD SVRHSCSVPY EVKFNPVGRE LYTHPPEHG VEQACQVYAH DAQNRGAYVE MHLPGSEVDS SLVSLSGSSV TVTPPDGTSA LVECECGGTK ISETINKTK QFSQCTKKEQ CRAYRLQNDK WVYNSDKLPK AAGATLKGKL HVPFLLADGK C TVPLAPEP MITFGFRSVS LKLHPKNPTY LITRQLADEP HYTHELISEP AVRNFTVTEK GW EFVWGNH PPKRFWAQET APGNPHGLPH EVITHYYHRY PMSTILGLSI CAAIATVSVA AST WLFCRS RVACLTPYRL TPNARIPFCL AVLCCARTAR AETTWESLDH LWNNNQQMFW IQLL IPLAA LIVVTRLLRC VCCVVPFLVM AGAAAPA YE HATTMPSQAG ISYNTIVNRA GYAPLP ISI TPTKIKLIPT VNLEYVTCHY KTGMDSPAIK CCGSQECTPT YRPDEQCKVF TGVYPFM WG GAYCFCDTEN TQVSKAYVMK SDDCLADHAE AYKAHTASVQ AFLNITVGEH SIVTTVYV N GETPVNFNGV KITAGPLSTA WTPFDRKIVQ YAGEIYNYDF PEYGAGQPGA FGDIQSRTV SSSDLYANTN LVLQRPKAGA IHVPYTQAPS GFEQWKKDKA PSLKFTAPFG CEIYTNPIRA ENCAVGSIP LAFDIPDALF TRVSETPTLS AAECTLNECV YSSDFGGIAT VKYSASKSGK C AVHVPSGT ATLKEAAVEL TEQGSATIHF STANIHPEFR LQICTSYVTC KGDCHPPKDH IV THPQYHA QTFTAAVSKT AWTWLTSLLG GSAVIIIIGL VLATIVAMYV LTNQKHN

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Macromolecule #2: Fab SKT20 heavy chain

MacromoleculeName: Fab SKT20 heavy chain / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: Macaca fascicularis (crab-eating macaque)
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: QVQVQESGPG LVKPSETLSL TCAVSSGSIN DDSYYWTWIR QSPGKGLEWL GFIHGGTGKS FYNPSLESRV TISKDTSRNQ FSLTLSSVSA ADTAVYYCAR SHFCSNTFCY GWFDVWGPGI RVTVSS AST KGPSVFPLAP SSRSTSESTA ALGCLVKDYF PEPVTVSWNS ...String:
QVQVQESGPG LVKPSETLSL TCAVSSGSIN DDSYYWTWIR QSPGKGLEWL GFIHGGTGKS FYNPSLESRV TISKDTSRNQ FSLTLSSVSA ADTAVYYCAR SHFCSNTFCY GWFDVWGPGI RVTVSS AST KGPSVFPLAP SSRSTSESTA ALGCLVKDYF PEPVTVSWNS GSLTSGVHTF PAVLQSSGLY SLSSVVTVPS SSLGTQTYVC NVNHKPSNTK VDKRVEIKTC Gglevlfq

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Macromolecule #3: Fab SKT20 light chain

MacromoleculeName: Fab SKT20 light chain / type: protein_or_peptide / ID: 3 / Enantiomer: LEVO
Source (natural)Organism: Macaca fascicularis (crab-eating macaque)
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: DVVMTQTPLS LPITPGEPAS ISCRSSQSLL HSNGNTYLHW YLQKPGQSPQ LLIYGGSNRA SGVPDRFSGS GSGTDFTLKI SKVEAEDVGV YYCVQAIAFP WTFGQGTKVE IK RTVAAPS VFIFPPSEDQ VKSGTVSVVC LLNNFYPREA SVKWKVDGAL KTGNSQESVT ...String:
DVVMTQTPLS LPITPGEPAS ISCRSSQSLL HSNGNTYLHW YLQKPGQSPQ LLIYGGSNRA SGVPDRFSGS GSGTDFTLKI SKVEAEDVGV YYCVQAIAFP WTFGQGTKVE IK RTVAAPS VFIFPPSEDQ VKSGTVSVVC LLNNFYPREA SVKWKVDGAL KTGNSQESVT EQDSKDNTYS LSSTLTLSST EYQSHKVYAC EVTHQGLSSP VTKSFNRGEC

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.25 µm / Nominal defocus min: 0.75 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 47.5 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 36125
Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final reconstructionApplied symmetry - Point group: I (icosahedral) / Resolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 11336
FSC plot (resolution estimation)

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