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Isometrically contracting insect asynchronous flight muscle quick frozen after a quick release step

by electron tomography, at 35 A resolution

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#1: Depositted structure unit, Image by Jmol

#2: Superimposing with simplified surface model of EM map, EMDB-1584, Image by Jmol

#3: Superimposing with simplified surface model of EM map, EMDB-1585, Image by Jmol

#4: With PDB-2w4u, Image by Jmol

#5: Superimposing with EM 3D map: EMDB-1585 (with PDB-2w4u), Image by UCSF CHIMERA

Entry
Summary
Database / IDPORTEIN DATA BANK (PDB) / 2w4v
TitleIsometrically contracting insect asynchronous flight muscle quick frozen after a quick release step
DescriptorMYOSIN HEAVY CHAIN
STRIATED MUSCLE
MYOSIN REGULATORY LIGHT CHAIN
STRIATED ADDUCTOR MUSCLE
MYOSIN ESSENTIAL LIGHT CHAIN
STRIATED ADDUCTOR MUSCLE
KeywordsCONTRACTILE PROTEIN, TROPOMYOSIN, LIGHT CHAINS, ACTIN-BINDING, ISOMETRIC CONTRACTION, THIN FILAMENT, MOTOR PROTEIN, THICK FILAMENT, MUSCLE PROTEIN
AuthorsWu, S., Liu, J., Reedy, M.C., Tregear, R.T., Winkler, H., Franzini-Armstrong, C., Sasaki, H., Lucaveche, C., Goldman, Y.E., Reedy, M.K., Taylor, K.A.
DateDeposition: 2008-12-02, Release: 2010-09-15
PDBj Mine pagesSummary, Structural Details, Experimental Details, Functional Details
Other databasesRCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Sequence details92 IDENTITY TO P24733 100 IDENTITY TO P13543 100 IDENTITY TO P07291
Structure Visualization
MoviesMovie Page

#1: Depositted structure unit, Image by Jmol

#2: Superimposing with simplified surface model of EM map, EMDB-1584, Image by Jmol

#3: Superimposing with simplified surface model of EM map, EMDB-1585, Image by Jmol

#4: With PDB-2w4u, Image by Jmol

#5: Superimposing with EM 3D map: EMDB-1585 (with PDB-2w4u), Image by UCSF CHIMERA

Structure viewersYorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries

EMDB-1584

CiteFit

EMDB-1585

CiteFit

Cite: data citing same article

Fit: target map of fitting

Article
Citation - primary
ArticlePLoS ONE, Vol. 7, Issue 6, Page e39422, Year 2012
TitleStructural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation.
AuthorsShenping Wu, Jun Liu, Mary C Reedy, Robert J Perz-Edwards, Richard T Tregear, Hanspeter Winkler, Clara Franzini-Armstrong, Hiroyuki Sasaki, Carmen Lucaveche, Yale E Goldman, Michael K Reedy, Kenneth A Taylor
Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America.
KeywordsActins (metabolism), Animals, Flight, Animal (physiology), Heteroptera (physiology), Isometric Contraction (physiology), Models, Biological, Muscle, Skeletal (physiology), Troponin (metabolism)
LinksDOI: 10.1371/journal.pone.0039422, PubMed: 22761792, PMC: PMC3382574
Citation - 1
ArticleJ. Struct. Biol., Vol. 168, Issue 3, Page 485-502, Year 2009
TitleMethods for identifying and averaging variable molecular conformations in tomograms of actively contracting insect flight muscle.
AuthorsShenping Wu, Jun Liu, Mary C Reedy, Hanspeter Winkler, Michael K Reedy, Kenneth A Taylor
Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306-4380, USA.
KeywordsAnimals, Electron Microscope Tomography (methods), Insects (ultrastructure), Muscle Contraction (physiology), Muscles (pathology)
LinksPII: S1047-8477(09)00234-2, DOI: 10.1016/j.jsb.2009.08.007, PubMed: 19698791, PMC: PMC2805068
Components
ID 1 : MYOSIN S1
Image
DescriptionMYOSIN HEAVY CHAIN, STRIATED MUSCLE
Typepolypeptide(L)
FragmentRESIDUES 5-835
Formula weight94844.594 Da
Number of molecules1
ID1
DetailsMYOSIN S1 HEAVY CHAIN
SourceMethod: Isolated from a natural source
Common name: BAY SCALLOP
NCBI taxonomy: ID:31199
Organism scientific: ARGOPECTEN IRRADIANS
LinksUniProt: P24733, Sequence view
ID 2 : MYOSIN S1, R-LC
Image
DescriptionMYOSIN REGULATORY LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE
Typepolypeptide(L)
FragmentRESIDUES 16-151
Formula weight15575.813 Da
Number of molecules1
ID2
DetailsMYOSIN REGULATORY LIGHT CHAIN, RLC, STRIATED ADDUCTOR MUSCLE
SourceMethod: Isolated from a natural source
Common name: BAY SCALLOP
NCBI taxonomy: ID:31199
Organism scientific: ARGOPECTEN IRRADIANS
LinksUniProt: P13543, Sequence view
ID 3 : MYOSIN S1, E-LC
Image
DescriptionMYOSIN ESSENTIAL LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE
Typepolypeptide(L)
FragmentRESIDUES 5-155
Formula weight17052.043 Da
Number of molecules1
ID3
DetailsMYOSIN ESSENTIAL LIGHT CHAIN, ELC, STRIATED ADDUCTOR MUSCLE
SourceMethod: Isolated from a natural source
Common name: BAY SCALLOP
NCBI taxonomy: ID:31199
Organism scientific: ARGOPECTEN IRRADIANS
LinksUniProt: P07291, Sequence view
Sample
Assembly
Aggregation stateTISSUE
DetailsTHIS SPECIMEN IS OBTAINED FROM A QUICK FROZEN, ISOMETRICALLY CONTRACTING ASYNCHRONOUS INSECT FLIGHT MUSCLE THAT HAS BEEN FREEZE SUBSTITUTED, PLASTIC EMBEDDED, AND THIN SECTIONED. THE FIBERS FOR THIS STUDY WERE SUBJECTED TO MECHANICAL TRANSIENTS. FOR THE QUICK RELEASE,THE FIBER WAS RELEASED 9 NM PER HALF-SARCOMERE IN 2 MS AND THE FREEZING IMPACT - OCC-6 MS LATER.
NameINSECT FIBRILLAR FLIGHT MUSCLE
Buffer
Name20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
Experiment
Reconstruction methodTOMOGRAPHY
Specimen typeFREEZE SUBSTITUTED INSECT FLIGHT MUSCLE
Sample support
DetailsCARBON
Vitrification
DetailsSMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
Experiment
MethodELECTRON MICROSCOPY
Electron Microscopy
Imaging
MicroscopeModel: FEI/PHILIPS CM300FEG/T
Electron gun
Electron sourceFIELD EMISSION GUN
Accelerating voltage300 kV
Illumination modeLOW DOSE-FLOOD BEAM
Lens
ModeBRIGHT FIELD
CsNominal: 2.0 mm
Specimen holder
Tilt angleMin: -72 degrees, Max: 72 degrees
Detector
TypeTIETZ TEM-CAM F224
Processing
3D reconstruction
DetailsNOTE THAT OUR LOWEST RESOLUTION DATA IS AT INVERSE 1 MICRON. NUMBER OF FOURIER COEFFICIENTS IS ALMOST A HALF MILLION. THESE COORDINATES WERE FITTED TO AVERAGED SUBVOLUMES OBTAINED FROM A DUAL AXIS TOMOGRAM. THE FITTING WAS DONE MANUALLY USING THE CRYSTALLOGRAPHIC MODEL FITTING PROGRAM O. THERE ARE 27 MODELS.
Refine
Refine idELECTRON MICROSCOPY
Ls d res high35.00 A
Refine hist
Cycle idLAST
Refine idELECTRON MICROSCOPY
D res high35.00
Total atoms8501
Protein atoms8501
Download
PDB format
Allpdb2w4v.ent.gz
pdb2w4v.ent (uncompressed file)
Header onlypdb2w4v.ent.gz
mmCIF format
mmCIF2w4v.cif.gz
XML format
All2w4v.xml.gz
No-atom2w4v-noatom.xml.gz
Ext-atom2w4v-extatom.xml.gz
Movie files
movie #1
.mp4 (H.264/MPEG-4 AVC format), 2.7 MB
.webm (WebM/VP8 format), 3.6 MB
movie #2
.mp4 (H.264/MPEG-4 AVC format), 3 MB
.webm (WebM/VP8 format), 3.6 MB
movie #3
.mp4 (H.264/MPEG-4 AVC format), 3.2 MB
.webm (WebM/VP8 format), 4.2 MB
movie #4
.mp4 (H.264/MPEG-4 AVC format), 2.6 MB
.webm (WebM/VP8 format), 3.5 MB
movie #5
.mp4 (H.264/MPEG-4 AVC format), 3.5 MB
.webm (WebM/VP8 format), 4.6 MB